In the context of current global warming,understanding urban thermal resilience(UTR)dynamics across dif-ferent climatic zones is crucial.This study aims to examine the complex interactions among urban morphology,green...In the context of current global warming,understanding urban thermal resilience(UTR)dynamics across dif-ferent climatic zones is crucial.This study aims to examine the complex interactions among urban morphology,green-blue infrastructure,and climate factors affecting UTR.Moving beyond traditional methods that compare urban and rural thermal differences,our research innovatively measures UTR by evaluating urban disturbances caused by extreme thermal events.To improve accuracy and reliability,we utilize an AI-powered Monte Carlo Simulation framework.Our findings emphasize the critical role of blue-green spaces in boosting UTR,whereas urban morphology often has a suppressive impact.Additionally,atmospheric humidity is identified as a critical factor affecting UTR.The study interestingly finds varied climatic responses:dense urban areas enhance resilience in arid and cold regions but reduce it in tropical and temperate zones.These findings highlight the need for a balance between sustainable urban living and infrastructure development.展开更多
PM_(2.5)constitutes a complex and diversemixture that significantly impacts the environment,human health,and climate change.However,existing observation and numerical simulation techniques have limitations,such as a l...PM_(2.5)constitutes a complex and diversemixture that significantly impacts the environment,human health,and climate change.However,existing observation and numerical simulation techniques have limitations,such as a lack of data,high acquisition costs,andmultiple uncertainties.These limitations hinder the acquisition of comprehensive information on PM_(2.5)chemical composition and effectively implement refined air pollution protection and control strategies.In this study,we developed an optimal deep learning model to acquire hourly mass concentrations of key PM_(2.5)chemical components without complex chemical analysis.The model was trained using a randomly partitioned multivariate dataset arranged in chronological order,including atmospheric state indicators,which previous studies did not consider.Our results showed that the correlation coefficients of key chemical components were no less than 0.96,and the root mean square errors ranged from 0.20 to 2.11μg/m^(3)for the entire process(training and testing combined).The model accurately captured the temporal characteristics of key chemical components,outperforming typical machine-learning models,previous studies,and global reanalysis datasets(such asModern-Era Retrospective analysis for Research and Applications,Version 2(MERRA-2)and Copernicus Atmosphere Monitoring Service ReAnalysis(CAMSRA)).We also quantified the feature importance using the random forest model,which showed that PM_(2.5),PM_(1),visibility,and temperature were the most influential variables for key chemical components.In conclusion,this study presents a practical approach to accurately obtain chemical composition information that can contribute to filling missing data,improved air pollution monitoring and source identification.This approach has the potential to enhance air pollution control strategies and promote public health and environmental sustainability.展开更多
An Ms 6.4 earthquake occurred in Yangbi,Yunnan,China on May 21,2021,which has obvious foreshock activity and abundant aftershocks.Based on the seismic observation data recorded by the Yunnan Seismic Network three days...An Ms 6.4 earthquake occurred in Yangbi,Yunnan,China on May 21,2021,which has obvious foreshock activity and abundant aftershocks.Based on the seismic observation data recorded by the Yunnan Seismic Network three days before and seven days after the mainshock,a doubledifference location method was used to relocate 2133 earthquakes of the Yangbi sequence.Aftershocks are mostly distributed to the southeast of the mainshock in a unilateral rupture pattern.This sequence exhibits a SE-trending linear alignment with a length of about 25 km,and most of the focal depth is above 12 km.Integrated with the seismic distribution and focal mechanism results,we infer that the strike of the seismogenic fault is about 140°,and dipping to the SW.The fault structure revealed by the seismic sequence is complex,with the NW segment exhibiting a steep dip and relatively simple structure of strike-slip rupture and the SE segment consisting of several branching ruptures.The Yangbi Earthquake is a typical foreshock-mainshock-aftershock sequence,and the mainshock is likely triggered by the largest foreshock.This earthquake occurred in the boundary between high-and lowvelocity anomalous zone,where is susceptible to generate large earthquakes.展开更多
Lithium-sulfur(Li-S)batteries are hampered by the infamous shuttle effect and slow redox kinetics,resulting in rapid capacity decay.Herein,a bifunctional catalysis CoB/BN@rGO with integrated structure and synergy effe...Lithium-sulfur(Li-S)batteries are hampered by the infamous shuttle effect and slow redox kinetics,resulting in rapid capacity decay.Herein,a bifunctional catalysis CoB/BN@rGO with integrated structure and synergy effect between adsorption and catalysis is proposed to solve the above problems.The integrated CoB and BN are simultaneously and uniformly introduced on the rGO substrate through a one-step calcination strategy,applied to modify the cathode side of PP separator.The transition metal borides can catalyze the conversion of lithium polysulfides(Li_(2)Sn,n≥4),whereas the bond of B-S is too weak to absorb LPS.Thus BN introduced can effectively restrict the diffusion of polysulfides via strong chemisorption with LiSnLi+…N,while the rGO substrate ensures smooth electron transfer for redox reaction.Therefore,through the integrated adsorption/catalysis,the shuttle effect is suppressed,the kinetics of redox reaction is enhanced,and the capacity decay is reduced.Using CoB/BN@rGO modified PP separator,the Li-S batteries with high initial capacity(1450 mAh g^(-1)at 0.35 mA cm^(-2))and long-cycle stability(700 cycles at 1.74 mA cm^(-2)with a decay rate of 0.032%per cycle)are achieved.This work provides a novel insight for the preparation of bifunctional catalysis with integrated structure for long-life Li-S batteries.展开更多
Jiaxing created a precedent using bypass riparian marshes to purify micro-polluted water sources in China.Pond-wetland complex with constructed root channel technology becomes a paradigm which can be analogized as&quo...Jiaxing created a precedent using bypass riparian marshes to purify micro-polluted water sources in China.Pond-wetland complex with constructed root channel technology becomes a paradigm which can be analogized as"human-body wetland model"based on bionics or biomimetics.Heterogeneous plant-bed/ditch system with highly active land/water ecotone interfaces,especially meandering boundaries,breeds many biochemical reactions"living areas".Optimization of hydraulic regulation promotes redox environment alternations and wetland treatment efficiency.Here we reported a series of upgrades and performances in Guanjinggang wetland after the Shijiuyang prototype.Morphological reform of plant-bed/ditch system played a vital role.Spatially root channel zone was main force of wetland purification,and temporally the treatment effect was higher in low-temperature seasons indicating non-temperature dependent mechanisms worked.Water pollution comprehensive index improved steadily from IV to III,and comprehensive pollution load was reduced by ca.40%–60%.Comprehensive evaluation function value further showed the gradients purification effect of the upgraded wetland.Ecological wetlands ameliorated source water quality,and reduced drinking water treatment reagents,thereby bringing about economic benefits.Through wetlands operation,people can see how the micro-polluted surface water becomes clear and clean,so promoting a significant social benefit.As a viable component of urban green space,wetlands could beautify regional eco-environment,freshen the air,increase urban ecological taste,and enhance the eco-environmental protection publicity.Thus,the multifunctional service values and indirect benefits are substantial.Jiaxing ecological wetlands provide a typical paradigm for water pollution remediation in developing countries and plays a leading role in technology engineering radiation effect.展开更多
Averrhoa carambola is commonly known as star fruit because of its peculiar shape,and its fruit is a rich source of minerals and vitamins.It is also used in traditional medicines in countries such as India,China,the Ph...Averrhoa carambola is commonly known as star fruit because of its peculiar shape,and its fruit is a rich source of minerals and vitamins.It is also used in traditional medicines in countries such as India,China,the Philippines,and Brazil for treating various ailments,including fever,diarrhea,vomiting,and skin disease.Here,we present the first draft genome of the Oxalidaceae family,with an assembled genome size of 470.51 Mb.In total,24,726 protein-coding genes were identified,and 16,490 genes were annotated using various well-known databases.The phylogenomic analysis confirmed the evolutionary position of the Oxalidaceae family.Based on the gene functional annotations,we also identified enzymes that may be involved in important nutritional pathways in the star fruit genome.Overall,the data from this first sequenced genome in the Oxalidaceae family provide an essential resource for nutritional,medicinal,and cultivational studies of the economically important star-fruit plant.展开更多
Objective:To determine the clinical characteristics and prognosis of primary tracheobronchial tumors(PTTs)in children,and to explore the most common tumor identification methods.Methods:The medical records of children...Objective:To determine the clinical characteristics and prognosis of primary tracheobronchial tumors(PTTs)in children,and to explore the most common tumor identification methods.Methods:The medical records of children with PTTs who were hospitalized at the Children's Hospital of Chongqing Medical University from January 1995 to January 2020 were reviewed retrospectively.The clinical features,imaging,treatments,and outcomes of these patients were statistically analyzed.Machine learning techniques such as Gaussian na?ve Bayes,support vector machine(SVM)and decision tree models were used to identify mucoepidermoid carcinoma(ME).Results:A total of 16 children were hospitalized with PTTs during the study period.This included 5(31.3%)children with ME,3(18.8%)children with inflammatory myofibroblastic tumors(IMT),2 children(12.5%)with sarcomas,2(12.5%)children with papillomatosis and 1 child(6.3%)each with carcinoid carcinoma,adenoid cystic carcinoma(ACC),hemangioma,and schwannoma,respectively.ME was the most common tumor type and amongst the 3 ME recognition methods,the SVM model showed the best performance.The main clinical symptoms of PPTs were cough(81.3%),breathlessness(50%),wheezing(43.8%),progressive dyspnea(37.5%),hemoptysis(37.5%),and fever(25%).Of the 16 patients,7 were treated with surgery,8 underwent bronchoscopic tumor resection,and 1 child died.Of the 11 other children,3 experienced recurrence,and the last 8 remained disease-free.No deaths were observed during the follow-up period.Conclusion:PTT are very rare in children and the highest percentage of cases is due to ME.The SVM model was highly accurate in identifying ME.Chest CT and bronchoscopy can effectively diagnose PTTs.Surgery and bronchoscopic intervention can both achieve good clinical results and the prognosis of the 11 children that were followed up was good.展开更多
We designed a disulfide-crosslinked mini-protein with a two-helical topology consisting of L-and Damino acids,which was exceptionally stable in serum.Therefore,we further used it as a scaffold to design mini-proteins ...We designed a disulfide-crosslinked mini-protein with a two-helical topology consisting of L-and Damino acids,which was exceptionally stable in serum.Therefore,we further used it as a scaffold to design mini-proteins targeting p53 positive tumor cells.Based on bifunctional grafting,key residues from the transactivation domain of p53 and a designed unnatural amino acid were grafted into the helix constituted by L-amino acids to confer the mini-protein with MDM2 inhibitory activity.Meanwhile,ten Arg residues were introduced to improve its membrane penetrating capacity.Among the mini-proteins,UPROL-10e showed nano-molar binding affinity on MDM2 and cellular toxicity on p53 expressing HCT116cells.展开更多
BACKGROUND Pancreatic neuroendocrine microtumors(PNEMTs)are small(<5 mm),nonfunctioning,well-differentiated neuroendocrine neoplasms.Although they are rare,they are not invariably benign.PNEMTs are typically discov...BACKGROUND Pancreatic neuroendocrine microtumors(PNEMTs)are small(<5 mm),nonfunctioning,well-differentiated neuroendocrine neoplasms.Although they are rare,they are not invariably benign.PNEMTs are typically discovered incidentally during autopsy.However,data regarding the occurrence of PNEMTs in the elderly population,particularly those identified incidentally in cadaveric studies,remain limited.AIM To investigate the prevalence and histopathological characteristics of PNEMTs in elderly individuals by analyzing cadaveric pancreatic tissues.METHODS We conducted a retrospective analysis of 85 pancreatic specimens(age range:58-109 years)obtained from cadavers for anatomical education and research at the Department of Life Dentistry,Nippon Dental University.Paraffin sections of the pancreatic head,body,and tail were prepared for histological and immunohistochemical analysis.RESULTS Five cases with PNEMTs(5/85,5.9%;male,n=33;female,n=52;mean age:85.8±12.1 years)were identified.The tumors were solitary,well circumscribed,and located within the pancreatic parenchyma(body:n=4;tail:n=1),and all were<5 mm(range:0.54-2.20 mm)in size.All tumors showed strong chromogranin A and synaptophysin positivity,and were predominantly glucagon(GLU)-positive.Ki-67 immunostaining indicated minimal proliferative activity;therefore,these tumors were considered non-functioning,GLU-producing,well-differentiated grade 1 PNEMTs.CONCLUSION Small,predominantly low-grade,GLU-secreting PNEMTs were present in 5.9%of elderly individuals,highlighting the prevalence of subclinical PNEMTs and the need for careful follow-up.展开更多
Traditional studies of microbial succession under iron-carbon composite(Fe-C)amendment application have focused on the entire microbial community,with limited attention to the responses and ecological roles of abundan...Traditional studies of microbial succession under iron-carbon composite(Fe-C)amendment application have focused on the entire microbial community,with limited attention to the responses and ecological roles of abundant or rare taxa.Herein,a 90-day microcosm incubation was conducted to investigate the effects of three Fe-C amendments,including Fe_(3)O_(4)-modified biochar(FeC-B),ferrihydrite-natural humic acid(FeC-N),and ferrihydrite-synthetic humic-like acid(FeC-S),on distribution patterns,assembly processes,and ecological functions of both abundant and rare subcommunities.Our results showed that Fe-C amendments significantly affected theα-diversity of rare taxa,particularly under FeC-B treatment,with minimal impact on abundant taxa.Fe-C amendments also reshaped the community structures of both groups.Rare taxa,representing 63.9%of Operational Taxonomic Unit(OTU)richness but only 1.6%of total abundance,played a key role in community diversity and were more susceptible to Fe-C amendments.Certain rare taxa transitioned to abundant status,demonstrating their potential as a microbial seed bank.Abundant taxa were positioned more centrally within the networks,and Fe-C applications promoted cooperative interactions between abundant and rare species.Deterministic processes dominated the assembly of the rare subcommunity,while stochastic processes primarily influenced the abundant bacterial community.Fe-C amendments reduced community differentiation among rare taxa while increasing variability among abundant groups.Functional diversity of rare groups surpassed that of abundant groups,with notable enhancement in nitrogen cycling-related genes under Fe-C treatments.This study highlights the complementary roles of abundant and rare taxa in soil remediation,providing insights for optimizing remediation strategies.展开更多
Early recognition is key to improving the prognosis of ischemic stroke(IS),while available imaging methods tend to target events that have already undergone ischemia.A new method to detect early IS is urgently needed,...Early recognition is key to improving the prognosis of ischemic stroke(IS),while available imaging methods tend to target events that have already undergone ischemia.A new method to detect early IS is urgently needed,as well as further study of its mechanisms.Viscosity and cysteine(Cys)levels of mitochondria have been associated with ferroptosis and IS.It is possible to identify IS and ferroptosis accurately and early by monitoring changes in mitochondrial Cys and viscosity simultaneously.In this work,a viscosity/Cys dual-responsive mitochondrial-targeted near-infrared(NIR)fluorescent probe(NVCP)was constructed for the precise tracking of IS using a two-dimensional design strategy.NVCP consists of a chromophore dyad containing diethylaminostyrene quinolinium rotor and chloro-sulfonylbenzoxadiazole(SBD-Cl)derivative with two easily distinguished emission bands(λ_(em)=592 and 670 nm).NVCP performs the way of killing two birds with one stone,that is,the probe exhibits excellent selectivity and sensitivity for detecting viscosity and Cys in living cells with excellent biocompatibility and accurate mitochondrial targeting capability by dual channel imaging mode.In addition,NVCP recognized that the viscosity increases and Cys level decreases in cells when undergoing ferroptosis and oxygen-glucose deprivation(OGD)stress by confocal imaging,flow cytometry,and Western blot experiments.Treatment of ferroptosis inhibitors(ferrostatin-1(Fer-1)and deferoxamine(DFO))could reverse the variation tendency of viscosity and Cys.This is the first time that the relationship between ferroptosis and IS was identified through an analysis of Cys and viscosity.More importantly,the ischemic area was also instantly distinguished from normal tissues through fluorescence imaging of NVCP in vivo.The developed NIR dual-responsive probe NVCP toward viscosity and Cys could serve as a sensitive and reliable tool for tracking ferroptosis-related pathological processes during IS.展开更多
BACKGROUND Acute cerebral infarction(ACI),a leading cause of death and disability,causes brain ischemia due to vessel blockage.Current time-limited interventions,such as clot removal,often fail to restore full functio...BACKGROUND Acute cerebral infarction(ACI),a leading cause of death and disability,causes brain ischemia due to vessel blockage.Current time-limited interventions,such as clot removal,often fail to restore full function.Neurorestoration is vital,but complicated.Vascular endothelial growth factor(VEGF)and basic fibroblast growth factor(bFGF)promote angiogenesis and neuroprotection.Stem cell therapy has potential to promote neurorestoration.Specifically,neural stem cells(NSC)reconstruct neural tissue,while mesenchymal stem cells(MSCs)provide support and secrete beneficial factors.Combining NSCs and MSCs in stem cell therapy may synergistically enhance ACI recovery,potentially via the regulation of VEGF and bFGF.However,the mechanisms underlying this combined approach remain unclear.AIM To investigate the therapeutic effect of combined NSC and MSC transplantation on neurological recovery and bFGF/VEGF expression in ACI patients.METHODS This study enrolled 156 patients with ACI treated from June 2022 to June 2023.Patients were randomly assigned to two groups:The control group(n=78)received conventional drug therapy,while the observation group(n=78)received conventional therapy and combined NSC and MSC transplantation.The following outcomes were compared between groups:National Institutes of Health Stroke Scale(NIHSS)score,Barthel index,cerebral perfusion and diffusion on magnetic resonance imaging,serum bFGF and VEGF levels,clinical efficacy,and adverse events.RESULTS Serum VEGF and bFGF levels negatively correlated with NIHSS scores in patients with ACI(r=-0.388,r=-0.239;P<0.05).The observation group(NSC and MSC)showed a significantly higher clinical efficacy of treatment than the controls(85.9%vs 69.2%;P<0.05).Both groups showed improved cerebral perfusion,increased Barthel index,and decreased NIHSS scores post-treatment(P<0.05),with significantly greater improvements in the observation group.Serum VEGF and bFGF levels increased significantly in both groups(P<0.05),but were higher in the observation group.Adverse events in the observation group(transient fever:4 cases;agitation:1 case;headache:2 cases)were mild and resolved with symptomatic treatment.Six-month follow-up revealed no abnormalities in magnetic resonance imaging,electrocardiogram,or blood tests.CONCLUSION NSC-MSC combination therapy enhances neurological function and cerebral perfusion in patients with ACI by upregulating VEGF and bFGF expression,demonstrating favorable clinical efficacy and safety.展开更多
Ferroptosis can serve as a potent strategy for regulating cell death via lipid peroxidation and the imbalance of the antioxidant system resulting from iron accumulation in triple-negative breast cancer(TNBC)therapy.Ho...Ferroptosis can serve as a potent strategy for regulating cell death via lipid peroxidation and the imbalance of the antioxidant system resulting from iron accumulation in triple-negative breast cancer(TNBC)therapy.However,the ferroptosis accompanied with down-regulation of glutathione peroxidase 4(GPX4)lead to CD36-mediated tumor-infiltrating CD8^(+)T cells uptaking fatty acids,resulting in the negative action on immunotherapeutic efficacy.Herein,the albumin nanoparticles,abbreviated as LHS NPs,were designed by co-assembly of hemin,linoleic acid-cystamine,and a CD36 inhibitor sulfosuccinimide oleate,to bi-directionally manipulated ferroptosis in tumor and CD8^(+)T cells for TNBC therapy.LHS NPs exerted more efficient reactive oxygen species generation,glutathione depletion and malondialdehyde production by the combinatory strategy of classical and non-classical ferroptosis modes,which amplified the positive action on ferroptosis in tumor cells.Meanwhile,LHS manipulated the negative action of ferroptosis by inhibiting the CD36 mediated-lipid peroxidation in CD8^(+)T cells,thereby activating the immunotherapeutic efficacy with the improvements on induction of immunogenic cell death,proliferation of CD4+CD8^(+)T cells and natural killer cells,alleviation immunosuppressive regulatory T cells and myeloid-derived suppressor cells,and repolarization of the M2-to M1-phenotype tumor-associated macrophages.Thus,LHS NPs demonstrated an improved antitumor efficacy in suppressing the tumor growth and lungmetastasis of 4T1-tumormice.Our work gives novel insights for the bi-directionally manipulating ferroptosis in tumor and CD8^(+)T cells on TNBC chemoimmunotherapy.展开更多
Different stress states have a significant influence on the magnitude of the microscopic plastic strain and result in the development of the microstructure evolution.As a result,a comprehensive understanding of the di...Different stress states have a significant influence on the magnitude of the microscopic plastic strain and result in the development of the microstructure evolution.As a result,a comprehensive understanding of the different scale variation on microstructure evolution during bending deformation is essential.The advanced high strength dual-phase(DP1180)steel was investigated using multiscale microstructure-based 3D representative volume element(RVE)modelling technology with emphasis on understanding the relationship between the microstructure,localised stress-strain evolution as well as the deformation characteristics in the bending process.It is demonstrated that the localised development in bending can be more accurately described by microscopic deformation when taking into account microstructural properties.Microstructure-based 3D RVEs from each chosen bending condition generally have comparable localisation properties,whilst the magnitudes and intensities differ.In addition,the most severe localised bands are predicted to occur close to the ferrite and martensite phase boundaries where the martensite grains are close together or have a somewhat sharp edge.The numerically predicted results for the microstructure evolution,shear bands development and stress and strain distribution after 3-point bending exhibit a good agreement with the relevant experimental observations.展开更多
BACKGROUND Irreversible electroporation(IRE)represents an innovative localized technique for tumor ablation,possessing the capacity to activate the immune response of the host.However,this method alone is inadequate t...BACKGROUND Irreversible electroporation(IRE)represents an innovative localized technique for tumor ablation,possessing the capacity to activate the immune response of the host.However,this method alone is inadequate to halt cancer progression,necessitating the integration of additional strategies to achieve effective immuno-therapy.AIM To investigate the effects and underlying mechanisms of antitumor immunity derived from the synergistic application of IRE and anti-programmed cell death protein 1(PD-1)therapy within a murine model of hepatocellular carcinoma.METHODS C57BL-6 mice with tumor growth were divided into four separate cohorts:Control group;IRE group;Anti-PD-1 group;And IRE+anti-PD-1 group.The infiltration levels of T,B,and natural killer cells within the tumors,as well as the plasma concentrations of T helper type 1 cytokines(interleukin-2,interferon-γ,and tumor necrosis factor-β),were evaluated.Real-time polymerase chain reaction was utilized to quantify the expression of cluster of differentiation(CD)8(a marker indicative of CD8+T cells)in the tumor specimens of the mice at various temporal intervals.Tumor growth trajectories were charted.RESULTS The results indicated that the IRE+anti-PD-1 group exhibited significantly heightened percentages of T lymphocyte infiltration,particularly CD4+and CD8+T cells,when compared to the control cohort.Additionally,this group displayed increased infiltration of natural killer and B cells,augmented cytokine levels,and elevated CD8 messenger RNA expression.A marked decrease in tumor volume was noted in the IRE+anti-PD-1 group,indicating enhanced therapeutic efficacy.CONCLUSION The combined application of IRE and checkpoint blockade elicits an antitumor immune response,leading to a more substantial reduction in tumor volume and improved therapeutic outcomes,thereby establishing a novel avenue for the ablation and immunotherapy of hepatocellular carcinoma.展开更多
Chronic obstructive pulmonary disease(COPD)is a complex condition marked by considerable interindividual heterogeneity.Comorbidities exacerbate this variability,worsening disease severity and reducing health-related q...Chronic obstructive pulmonary disease(COPD)is a complex condition marked by considerable interindividual heterogeneity.Comorbidities exacerbate this variability,worsening disease severity and reducing health-related quality of life(HRQoL).Despite the high prevalence of COPD in China,COPD patient clusters remain poorly characterized.This study aimed to identify and validate clusters of Chinese patients with COPD,characterized primarily by comorbidity profiles,using cluster analysis.This cross-sectional,multicenter cohort study used data from the Chinese Enjoying Breathing Program(2020–2023).HRQoL was measured using the EuroQol five dimension(EQ-5D).Dimension reduction was performed via multiple correspondence analysis on 31 variables,including indicators of 27 comorbidities and four sociodemographic or health-related characteristics.Unsupervised machine learning algorithms,K-means++,and hierarchical clustering identified distinct clusters.Robustness was assessed using random forest classification.Logistic regression evaluated the association between cluster membership and EQ-5D outcomes.Among 11145 patients,59.4%had comorbidities.Four clusters emerged:young male smokers,biomass-exposed females,respiratory comorbidity,and elderly multimorbid.The last two clusters had notably lower HRQoL.Cluster analysis identified four clinically meaningful COPD patient clusters based on comorbidities and risk profiles,each with distinct HRQoL outcomes.These findings support targeted public health interventions and integrated care strategies for COPD management.展开更多
Formaldehyde(HCHO)is a high-yield product of the oxidation of volatile organic compounds(VOCs)released by anthropogenic activities,fires,and vegetations.Hence,we examined the spatiotemporal variation trends in HCHO co...Formaldehyde(HCHO)is a high-yield product of the oxidation of volatile organic compounds(VOCs)released by anthropogenic activities,fires,and vegetations.Hence,we examined the spatiotemporal variation trends in HCHO columns observed using the Ozone Monitoring Instrument(OMI)during 2005–2021 across the Fenwei Plain(FWP)and analysed the source and variability of HCHO using multi-source data,such as thermal anomalies.The spatial distribution of the annualmean HCHO in the FWP increased from northwest to southeast during 2005–2021,and the high-value aggregation areas contracted and gradually clustered,forming a belt-shaped distribution area from Xi’an to Baoji,north of the Qinling Mountains.The annual mean HCHO concentration generally showed a two-step increase over the 17 years.Fires showed a single-peak trend in March and a double-peak M-shaped trend in March and October,whereas urban thermal anomalies(UTAs)showed an inverted U-shaped trend over 17 years,with peaks occurring in May.The HCHO peaks are mainly caused by the alternating contributions of fires and UTAs.The fires and UTAs(predominantly industrial heat sources)played a role in controlling the background level of HCHO in the FWP.Precipitation and temperature were also important influencing variables for seasonal variations,and the influence of plant sources on HCHO concentrations had significant regional characteristics and contributions.In addition,the FWP has poor dispersion conditions and is an aggregated area for the long-range transport of air pollutants.展开更多
基金financed by‘Data Analysis of Thermal Environment and Low-Carbon Intelligent Optimization Design of Urban Ecological Layout’s Impact’under National Natural Science Foundation of China(Grant No.524B200113)‘Basic Theory of Sustainable Urban Planning,Construction,and Governance’under the 14th Five-Year Plan of the State Key Research and Development Program of the People’s Republic of China(Grant No.2022YFC3800205)+1 种基金‘Key Technologies for Regional Carbon Neutral Mega-City Planning and Design’under Shanghai Science and Technology Support Program for Carbon(Grant No.22DZ1207800)Shanghai Intelligent Science and Technology IV Summit Discipline‘Cross-Innovation Science and Education Integration Fund’.
文摘In the context of current global warming,understanding urban thermal resilience(UTR)dynamics across dif-ferent climatic zones is crucial.This study aims to examine the complex interactions among urban morphology,green-blue infrastructure,and climate factors affecting UTR.Moving beyond traditional methods that compare urban and rural thermal differences,our research innovatively measures UTR by evaluating urban disturbances caused by extreme thermal events.To improve accuracy and reliability,we utilize an AI-powered Monte Carlo Simulation framework.Our findings emphasize the critical role of blue-green spaces in boosting UTR,whereas urban morphology often has a suppressive impact.Additionally,atmospheric humidity is identified as a critical factor affecting UTR.The study interestingly finds varied climatic responses:dense urban areas enhance resilience in arid and cold regions but reduce it in tropical and temperate zones.These findings highlight the need for a balance between sustainable urban living and infrastructure development.
基金supported by the National Key Research and Development Program for Young Scientists of China(No.2022YFC3704000)the National Natural Science Foundation of China(No.42275122)the National Key Scientific and Technological Infrastructure project“Earth System Science Numerical Simulator Facility”(EarthLab).
文摘PM_(2.5)constitutes a complex and diversemixture that significantly impacts the environment,human health,and climate change.However,existing observation and numerical simulation techniques have limitations,such as a lack of data,high acquisition costs,andmultiple uncertainties.These limitations hinder the acquisition of comprehensive information on PM_(2.5)chemical composition and effectively implement refined air pollution protection and control strategies.In this study,we developed an optimal deep learning model to acquire hourly mass concentrations of key PM_(2.5)chemical components without complex chemical analysis.The model was trained using a randomly partitioned multivariate dataset arranged in chronological order,including atmospheric state indicators,which previous studies did not consider.Our results showed that the correlation coefficients of key chemical components were no less than 0.96,and the root mean square errors ranged from 0.20 to 2.11μg/m^(3)for the entire process(training and testing combined).The model accurately captured the temporal characteristics of key chemical components,outperforming typical machine-learning models,previous studies,and global reanalysis datasets(such asModern-Era Retrospective analysis for Research and Applications,Version 2(MERRA-2)and Copernicus Atmosphere Monitoring Service ReAnalysis(CAMSRA)).We also quantified the feature importance using the random forest model,which showed that PM_(2.5),PM_(1),visibility,and temperature were the most influential variables for key chemical components.In conclusion,this study presents a practical approach to accurately obtain chemical composition information that can contribute to filling missing data,improved air pollution monitoring and source identification.This approach has the potential to enhance air pollution control strategies and promote public health and environmental sustainability.
基金financially supported by National Science Foundation of China(No.41774067)the National Key R&D Program of China(No.2018YFC1503400)+1 种基金the Special Fund of the Institute of GeophysicsChina Earthquake Administration(No.DQJB20X07)。
文摘An Ms 6.4 earthquake occurred in Yangbi,Yunnan,China on May 21,2021,which has obvious foreshock activity and abundant aftershocks.Based on the seismic observation data recorded by the Yunnan Seismic Network three days before and seven days after the mainshock,a doubledifference location method was used to relocate 2133 earthquakes of the Yangbi sequence.Aftershocks are mostly distributed to the southeast of the mainshock in a unilateral rupture pattern.This sequence exhibits a SE-trending linear alignment with a length of about 25 km,and most of the focal depth is above 12 km.Integrated with the seismic distribution and focal mechanism results,we infer that the strike of the seismogenic fault is about 140°,and dipping to the SW.The fault structure revealed by the seismic sequence is complex,with the NW segment exhibiting a steep dip and relatively simple structure of strike-slip rupture and the SE segment consisting of several branching ruptures.The Yangbi Earthquake is a typical foreshock-mainshock-aftershock sequence,and the mainshock is likely triggered by the largest foreshock.This earthquake occurred in the boundary between high-and lowvelocity anomalous zone,where is susceptible to generate large earthquakes.
基金supported by the National Natural Science Foundation of China(22078228)。
文摘Lithium-sulfur(Li-S)batteries are hampered by the infamous shuttle effect and slow redox kinetics,resulting in rapid capacity decay.Herein,a bifunctional catalysis CoB/BN@rGO with integrated structure and synergy effect between adsorption and catalysis is proposed to solve the above problems.The integrated CoB and BN are simultaneously and uniformly introduced on the rGO substrate through a one-step calcination strategy,applied to modify the cathode side of PP separator.The transition metal borides can catalyze the conversion of lithium polysulfides(Li_(2)Sn,n≥4),whereas the bond of B-S is too weak to absorb LPS.Thus BN introduced can effectively restrict the diffusion of polysulfides via strong chemisorption with LiSnLi+…N,while the rGO substrate ensures smooth electron transfer for redox reaction.Therefore,through the integrated adsorption/catalysis,the shuttle effect is suppressed,the kinetics of redox reaction is enhanced,and the capacity decay is reduced.Using CoB/BN@rGO modified PP separator,the Li-S batteries with high initial capacity(1450 mAh g^(-1)at 0.35 mA cm^(-2))and long-cycle stability(700 cycles at 1.74 mA cm^(-2)with a decay rate of 0.032%per cycle)are achieved.This work provides a novel insight for the preparation of bifunctional catalysis with integrated structure for long-life Li-S batteries.
基金supported by the National Science and Technology Major Project for Water Pollution Control and Treatment(Nos.2017ZX07201004–002,2012ZX07403–003–03,2008ZX07421–001–04)National Natural Science Foundation of China(No.51278487)。
文摘Jiaxing created a precedent using bypass riparian marshes to purify micro-polluted water sources in China.Pond-wetland complex with constructed root channel technology becomes a paradigm which can be analogized as"human-body wetland model"based on bionics or biomimetics.Heterogeneous plant-bed/ditch system with highly active land/water ecotone interfaces,especially meandering boundaries,breeds many biochemical reactions"living areas".Optimization of hydraulic regulation promotes redox environment alternations and wetland treatment efficiency.Here we reported a series of upgrades and performances in Guanjinggang wetland after the Shijiuyang prototype.Morphological reform of plant-bed/ditch system played a vital role.Spatially root channel zone was main force of wetland purification,and temporally the treatment effect was higher in low-temperature seasons indicating non-temperature dependent mechanisms worked.Water pollution comprehensive index improved steadily from IV to III,and comprehensive pollution load was reduced by ca.40%–60%.Comprehensive evaluation function value further showed the gradients purification effect of the upgraded wetland.Ecological wetlands ameliorated source water quality,and reduced drinking water treatment reagents,thereby bringing about economic benefits.Through wetlands operation,people can see how the micro-polluted surface water becomes clear and clean,so promoting a significant social benefit.As a viable component of urban green space,wetlands could beautify regional eco-environment,freshen the air,increase urban ecological taste,and enhance the eco-environmental protection publicity.Thus,the multifunctional service values and indirect benefits are substantial.Jiaxing ecological wetlands provide a typical paradigm for water pollution remediation in developing countries and plays a leading role in technology engineering radiation effect.
基金supported by funding from the National Key R&D Program of China(No.2019YFC1711000)the Shenzhen Municipal Government of China(grants JCYJ20170817145512476 and JCYJ20160510141910129)+1 种基金the Guangdong Provincial Key Laboratory of Genome Read and Write(grant 2017B030301011)the NMPA Key Laboratory for the Rapid Testing Technology of Drugs.
文摘Averrhoa carambola is commonly known as star fruit because of its peculiar shape,and its fruit is a rich source of minerals and vitamins.It is also used in traditional medicines in countries such as India,China,the Philippines,and Brazil for treating various ailments,including fever,diarrhea,vomiting,and skin disease.Here,we present the first draft genome of the Oxalidaceae family,with an assembled genome size of 470.51 Mb.In total,24,726 protein-coding genes were identified,and 16,490 genes were annotated using various well-known databases.The phylogenomic analysis confirmed the evolutionary position of the Oxalidaceae family.Based on the gene functional annotations,we also identified enzymes that may be involved in important nutritional pathways in the star fruit genome.Overall,the data from this first sequenced genome in the Oxalidaceae family provide an essential resource for nutritional,medicinal,and cultivational studies of the economically important star-fruit plant.
基金supported by the Chongqing Science and Health Joint Medical Research Project(No.8187011078).
文摘Objective:To determine the clinical characteristics and prognosis of primary tracheobronchial tumors(PTTs)in children,and to explore the most common tumor identification methods.Methods:The medical records of children with PTTs who were hospitalized at the Children's Hospital of Chongqing Medical University from January 1995 to January 2020 were reviewed retrospectively.The clinical features,imaging,treatments,and outcomes of these patients were statistically analyzed.Machine learning techniques such as Gaussian na?ve Bayes,support vector machine(SVM)and decision tree models were used to identify mucoepidermoid carcinoma(ME).Results:A total of 16 children were hospitalized with PTTs during the study period.This included 5(31.3%)children with ME,3(18.8%)children with inflammatory myofibroblastic tumors(IMT),2 children(12.5%)with sarcomas,2(12.5%)children with papillomatosis and 1 child(6.3%)each with carcinoid carcinoma,adenoid cystic carcinoma(ACC),hemangioma,and schwannoma,respectively.ME was the most common tumor type and amongst the 3 ME recognition methods,the SVM model showed the best performance.The main clinical symptoms of PPTs were cough(81.3%),breathlessness(50%),wheezing(43.8%),progressive dyspnea(37.5%),hemoptysis(37.5%),and fever(25%).Of the 16 patients,7 were treated with surgery,8 underwent bronchoscopic tumor resection,and 1 child died.Of the 11 other children,3 experienced recurrence,and the last 8 remained disease-free.No deaths were observed during the follow-up period.Conclusion:PTT are very rare in children and the highest percentage of cases is due to ME.The SVM model was highly accurate in identifying ME.Chest CT and bronchoscopy can effectively diagnose PTTs.Surgery and bronchoscopic intervention can both achieve good clinical results and the prognosis of the 11 children that were followed up was good.
基金supported by the National Natural Science Foundation of China(Nos.3217110331 and 8212200560)Major new drug development in Shandong Province(No.2020CXGC010503)。
文摘We designed a disulfide-crosslinked mini-protein with a two-helical topology consisting of L-and Damino acids,which was exceptionally stable in serum.Therefore,we further used it as a scaffold to design mini-proteins targeting p53 positive tumor cells.Based on bifunctional grafting,key residues from the transactivation domain of p53 and a designed unnatural amino acid were grafted into the helix constituted by L-amino acids to confer the mini-protein with MDM2 inhibitory activity.Meanwhile,ten Arg residues were introduced to improve its membrane penetrating capacity.Among the mini-proteins,UPROL-10e showed nano-molar binding affinity on MDM2 and cellular toxicity on p53 expressing HCT116cells.
基金Supported by Grant-in-Aid for Scientific Research from the Ministry of Education,Culture,Sports,Science and Technology of Japan,No.25K10147.
文摘BACKGROUND Pancreatic neuroendocrine microtumors(PNEMTs)are small(<5 mm),nonfunctioning,well-differentiated neuroendocrine neoplasms.Although they are rare,they are not invariably benign.PNEMTs are typically discovered incidentally during autopsy.However,data regarding the occurrence of PNEMTs in the elderly population,particularly those identified incidentally in cadaveric studies,remain limited.AIM To investigate the prevalence and histopathological characteristics of PNEMTs in elderly individuals by analyzing cadaveric pancreatic tissues.METHODS We conducted a retrospective analysis of 85 pancreatic specimens(age range:58-109 years)obtained from cadavers for anatomical education and research at the Department of Life Dentistry,Nippon Dental University.Paraffin sections of the pancreatic head,body,and tail were prepared for histological and immunohistochemical analysis.RESULTS Five cases with PNEMTs(5/85,5.9%;male,n=33;female,n=52;mean age:85.8±12.1 years)were identified.The tumors were solitary,well circumscribed,and located within the pancreatic parenchyma(body:n=4;tail:n=1),and all were<5 mm(range:0.54-2.20 mm)in size.All tumors showed strong chromogranin A and synaptophysin positivity,and were predominantly glucagon(GLU)-positive.Ki-67 immunostaining indicated minimal proliferative activity;therefore,these tumors were considered non-functioning,GLU-producing,well-differentiated grade 1 PNEMTs.CONCLUSION Small,predominantly low-grade,GLU-secreting PNEMTs were present in 5.9%of elderly individuals,highlighting the prevalence of subclinical PNEMTs and the need for careful follow-up.
基金supported by the National Natural Science Foundation of China(No.42007128)the Fundamental Research Funds for the Central Universities(No.2024QNYL30)the Graduate Research and Practice Projects of Minzu University of China(No.SZKY2024034).
文摘Traditional studies of microbial succession under iron-carbon composite(Fe-C)amendment application have focused on the entire microbial community,with limited attention to the responses and ecological roles of abundant or rare taxa.Herein,a 90-day microcosm incubation was conducted to investigate the effects of three Fe-C amendments,including Fe_(3)O_(4)-modified biochar(FeC-B),ferrihydrite-natural humic acid(FeC-N),and ferrihydrite-synthetic humic-like acid(FeC-S),on distribution patterns,assembly processes,and ecological functions of both abundant and rare subcommunities.Our results showed that Fe-C amendments significantly affected theα-diversity of rare taxa,particularly under FeC-B treatment,with minimal impact on abundant taxa.Fe-C amendments also reshaped the community structures of both groups.Rare taxa,representing 63.9%of Operational Taxonomic Unit(OTU)richness but only 1.6%of total abundance,played a key role in community diversity and were more susceptible to Fe-C amendments.Certain rare taxa transitioned to abundant status,demonstrating their potential as a microbial seed bank.Abundant taxa were positioned more centrally within the networks,and Fe-C applications promoted cooperative interactions between abundant and rare species.Deterministic processes dominated the assembly of the rare subcommunity,while stochastic processes primarily influenced the abundant bacterial community.Fe-C amendments reduced community differentiation among rare taxa while increasing variability among abundant groups.Functional diversity of rare groups surpassed that of abundant groups,with notable enhancement in nitrogen cycling-related genes under Fe-C treatments.This study highlights the complementary roles of abundant and rare taxa in soil remediation,providing insights for optimizing remediation strategies.
基金supported by The Natural Science Foundation of Hunan Province(Nos.2022JJ30484,2022JJ10042,2023JJ50157)The Start-up funds of University of South China(Nos.201RGC012,20XQD030)+3 种基金The Research Foundation of Education Bureau of Hunan Province(No.22B0418)Education Ministry’s Collaborative Education Program with Industry of China(Nos.220902102225257,230902999244208)Ministry of Education of China:“Chunhui Plan”(No.HZKY20220359)The Open Fund of State Key Laboratory of Chemo/Biosensing and Chemometrics(Nos.2021013,2021015).
文摘Early recognition is key to improving the prognosis of ischemic stroke(IS),while available imaging methods tend to target events that have already undergone ischemia.A new method to detect early IS is urgently needed,as well as further study of its mechanisms.Viscosity and cysteine(Cys)levels of mitochondria have been associated with ferroptosis and IS.It is possible to identify IS and ferroptosis accurately and early by monitoring changes in mitochondrial Cys and viscosity simultaneously.In this work,a viscosity/Cys dual-responsive mitochondrial-targeted near-infrared(NIR)fluorescent probe(NVCP)was constructed for the precise tracking of IS using a two-dimensional design strategy.NVCP consists of a chromophore dyad containing diethylaminostyrene quinolinium rotor and chloro-sulfonylbenzoxadiazole(SBD-Cl)derivative with two easily distinguished emission bands(λ_(em)=592 and 670 nm).NVCP performs the way of killing two birds with one stone,that is,the probe exhibits excellent selectivity and sensitivity for detecting viscosity and Cys in living cells with excellent biocompatibility and accurate mitochondrial targeting capability by dual channel imaging mode.In addition,NVCP recognized that the viscosity increases and Cys level decreases in cells when undergoing ferroptosis and oxygen-glucose deprivation(OGD)stress by confocal imaging,flow cytometry,and Western blot experiments.Treatment of ferroptosis inhibitors(ferrostatin-1(Fer-1)and deferoxamine(DFO))could reverse the variation tendency of viscosity and Cys.This is the first time that the relationship between ferroptosis and IS was identified through an analysis of Cys and viscosity.More importantly,the ischemic area was also instantly distinguished from normal tissues through fluorescence imaging of NVCP in vivo.The developed NIR dual-responsive probe NVCP toward viscosity and Cys could serve as a sensitive and reliable tool for tracking ferroptosis-related pathological processes during IS.
文摘BACKGROUND Acute cerebral infarction(ACI),a leading cause of death and disability,causes brain ischemia due to vessel blockage.Current time-limited interventions,such as clot removal,often fail to restore full function.Neurorestoration is vital,but complicated.Vascular endothelial growth factor(VEGF)and basic fibroblast growth factor(bFGF)promote angiogenesis and neuroprotection.Stem cell therapy has potential to promote neurorestoration.Specifically,neural stem cells(NSC)reconstruct neural tissue,while mesenchymal stem cells(MSCs)provide support and secrete beneficial factors.Combining NSCs and MSCs in stem cell therapy may synergistically enhance ACI recovery,potentially via the regulation of VEGF and bFGF.However,the mechanisms underlying this combined approach remain unclear.AIM To investigate the therapeutic effect of combined NSC and MSC transplantation on neurological recovery and bFGF/VEGF expression in ACI patients.METHODS This study enrolled 156 patients with ACI treated from June 2022 to June 2023.Patients were randomly assigned to two groups:The control group(n=78)received conventional drug therapy,while the observation group(n=78)received conventional therapy and combined NSC and MSC transplantation.The following outcomes were compared between groups:National Institutes of Health Stroke Scale(NIHSS)score,Barthel index,cerebral perfusion and diffusion on magnetic resonance imaging,serum bFGF and VEGF levels,clinical efficacy,and adverse events.RESULTS Serum VEGF and bFGF levels negatively correlated with NIHSS scores in patients with ACI(r=-0.388,r=-0.239;P<0.05).The observation group(NSC and MSC)showed a significantly higher clinical efficacy of treatment than the controls(85.9%vs 69.2%;P<0.05).Both groups showed improved cerebral perfusion,increased Barthel index,and decreased NIHSS scores post-treatment(P<0.05),with significantly greater improvements in the observation group.Serum VEGF and bFGF levels increased significantly in both groups(P<0.05),but were higher in the observation group.Adverse events in the observation group(transient fever:4 cases;agitation:1 case;headache:2 cases)were mild and resolved with symptomatic treatment.Six-month follow-up revealed no abnormalities in magnetic resonance imaging,electrocardiogram,or blood tests.CONCLUSION NSC-MSC combination therapy enhances neurological function and cerebral perfusion in patients with ACI by upregulating VEGF and bFGF expression,demonstrating favorable clinical efficacy and safety.
基金supported by the National Nature Science Foundation of China(NO.82260699)the Science and Technology Leading Talents of Ningxia(NO.2022GKLRLX011)the West Light Foundation of The Chinese Academy of Sciences(the Science and Technology Department of Ningxia,Department of Science and Technology Cooperation[2021]NO.2).
文摘Ferroptosis can serve as a potent strategy for regulating cell death via lipid peroxidation and the imbalance of the antioxidant system resulting from iron accumulation in triple-negative breast cancer(TNBC)therapy.However,the ferroptosis accompanied with down-regulation of glutathione peroxidase 4(GPX4)lead to CD36-mediated tumor-infiltrating CD8^(+)T cells uptaking fatty acids,resulting in the negative action on immunotherapeutic efficacy.Herein,the albumin nanoparticles,abbreviated as LHS NPs,were designed by co-assembly of hemin,linoleic acid-cystamine,and a CD36 inhibitor sulfosuccinimide oleate,to bi-directionally manipulated ferroptosis in tumor and CD8^(+)T cells for TNBC therapy.LHS NPs exerted more efficient reactive oxygen species generation,glutathione depletion and malondialdehyde production by the combinatory strategy of classical and non-classical ferroptosis modes,which amplified the positive action on ferroptosis in tumor cells.Meanwhile,LHS manipulated the negative action of ferroptosis by inhibiting the CD36 mediated-lipid peroxidation in CD8^(+)T cells,thereby activating the immunotherapeutic efficacy with the improvements on induction of immunogenic cell death,proliferation of CD4+CD8^(+)T cells and natural killer cells,alleviation immunosuppressive regulatory T cells and myeloid-derived suppressor cells,and repolarization of the M2-to M1-phenotype tumor-associated macrophages.Thus,LHS NPs demonstrated an improved antitumor efficacy in suppressing the tumor growth and lungmetastasis of 4T1-tumormice.Our work gives novel insights for the bi-directionally manipulating ferroptosis in tumor and CD8^(+)T cells on TNBC chemoimmunotherapy.
基金supported by HBIS Group under the Grant No.IRIS 200506003.
文摘Different stress states have a significant influence on the magnitude of the microscopic plastic strain and result in the development of the microstructure evolution.As a result,a comprehensive understanding of the different scale variation on microstructure evolution during bending deformation is essential.The advanced high strength dual-phase(DP1180)steel was investigated using multiscale microstructure-based 3D representative volume element(RVE)modelling technology with emphasis on understanding the relationship between the microstructure,localised stress-strain evolution as well as the deformation characteristics in the bending process.It is demonstrated that the localised development in bending can be more accurately described by microscopic deformation when taking into account microstructural properties.Microstructure-based 3D RVEs from each chosen bending condition generally have comparable localisation properties,whilst the magnitudes and intensities differ.In addition,the most severe localised bands are predicted to occur close to the ferrite and martensite phase boundaries where the martensite grains are close together or have a somewhat sharp edge.The numerically predicted results for the microstructure evolution,shear bands development and stress and strain distribution after 3-point bending exhibit a good agreement with the relevant experimental observations.
基金Supported by the Science and Technology Program of Guangzhou,No.202201020024.
文摘BACKGROUND Irreversible electroporation(IRE)represents an innovative localized technique for tumor ablation,possessing the capacity to activate the immune response of the host.However,this method alone is inadequate to halt cancer progression,necessitating the integration of additional strategies to achieve effective immuno-therapy.AIM To investigate the effects and underlying mechanisms of antitumor immunity derived from the synergistic application of IRE and anti-programmed cell death protein 1(PD-1)therapy within a murine model of hepatocellular carcinoma.METHODS C57BL-6 mice with tumor growth were divided into four separate cohorts:Control group;IRE group;Anti-PD-1 group;And IRE+anti-PD-1 group.The infiltration levels of T,B,and natural killer cells within the tumors,as well as the plasma concentrations of T helper type 1 cytokines(interleukin-2,interferon-γ,and tumor necrosis factor-β),were evaluated.Real-time polymerase chain reaction was utilized to quantify the expression of cluster of differentiation(CD)8(a marker indicative of CD8+T cells)in the tumor specimens of the mice at various temporal intervals.Tumor growth trajectories were charted.RESULTS The results indicated that the IRE+anti-PD-1 group exhibited significantly heightened percentages of T lymphocyte infiltration,particularly CD4+and CD8+T cells,when compared to the control cohort.Additionally,this group displayed increased infiltration of natural killer and B cells,augmented cytokine levels,and elevated CD8 messenger RNA expression.A marked decrease in tumor volume was noted in the IRE+anti-PD-1 group,indicating enhanced therapeutic efficacy.CONCLUSION The combined application of IRE and checkpoint blockade elicits an antitumor immune response,leading to a more substantial reduction in tumor volume and improved therapeutic outcomes,thereby establishing a novel avenue for the ablation and immunotherapy of hepatocellular carcinoma.
基金supported by the Ministry of Science and Tech-nology of the People’s Republic of China(2023ZD0506000)the CAMS Innovation Fund for Medical Sciences(ClFMS,2023-I2M-2-001)the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences(2022-ZHCH330-01).The statements made and views expressed are solely the responsibility of the authors.
文摘Chronic obstructive pulmonary disease(COPD)is a complex condition marked by considerable interindividual heterogeneity.Comorbidities exacerbate this variability,worsening disease severity and reducing health-related quality of life(HRQoL).Despite the high prevalence of COPD in China,COPD patient clusters remain poorly characterized.This study aimed to identify and validate clusters of Chinese patients with COPD,characterized primarily by comorbidity profiles,using cluster analysis.This cross-sectional,multicenter cohort study used data from the Chinese Enjoying Breathing Program(2020–2023).HRQoL was measured using the EuroQol five dimension(EQ-5D).Dimension reduction was performed via multiple correspondence analysis on 31 variables,including indicators of 27 comorbidities and four sociodemographic or health-related characteristics.Unsupervised machine learning algorithms,K-means++,and hierarchical clustering identified distinct clusters.Robustness was assessed using random forest classification.Logistic regression evaluated the association between cluster membership and EQ-5D outcomes.Among 11145 patients,59.4%had comorbidities.Four clusters emerged:young male smokers,biomass-exposed females,respiratory comorbidity,and elderly multimorbid.The last two clusters had notably lower HRQoL.Cluster analysis identified four clinically meaningful COPD patient clusters based on comorbidities and risk profiles,each with distinct HRQoL outcomes.These findings support targeted public health interventions and integrated care strategies for COPD management.
基金supported by the National Natural Science Foundation of China(No.41571062)the Fundamental Research Funds for the Central Universities(No.2021TS014)the Natural Science Basic Research Plan in Shaanxi Province of China(No.2023-JC-YB-259).
文摘Formaldehyde(HCHO)is a high-yield product of the oxidation of volatile organic compounds(VOCs)released by anthropogenic activities,fires,and vegetations.Hence,we examined the spatiotemporal variation trends in HCHO columns observed using the Ozone Monitoring Instrument(OMI)during 2005–2021 across the Fenwei Plain(FWP)and analysed the source and variability of HCHO using multi-source data,such as thermal anomalies.The spatial distribution of the annualmean HCHO in the FWP increased from northwest to southeast during 2005–2021,and the high-value aggregation areas contracted and gradually clustered,forming a belt-shaped distribution area from Xi’an to Baoji,north of the Qinling Mountains.The annual mean HCHO concentration generally showed a two-step increase over the 17 years.Fires showed a single-peak trend in March and a double-peak M-shaped trend in March and October,whereas urban thermal anomalies(UTAs)showed an inverted U-shaped trend over 17 years,with peaks occurring in May.The HCHO peaks are mainly caused by the alternating contributions of fires and UTAs.The fires and UTAs(predominantly industrial heat sources)played a role in controlling the background level of HCHO in the FWP.Precipitation and temperature were also important influencing variables for seasonal variations,and the influence of plant sources on HCHO concentrations had significant regional characteristics and contributions.In addition,the FWP has poor dispersion conditions and is an aggregated area for the long-range transport of air pollutants.
