Soil thickness,intended as depth to bedrock,is a key input parameter for many environmental models.Nevertheless,it is often difficult to obtain a reliable spatially exhaustive soil thickness map in widearea applicatio...Soil thickness,intended as depth to bedrock,is a key input parameter for many environmental models.Nevertheless,it is often difficult to obtain a reliable spatially exhaustive soil thickness map in widearea applications,and existing prediction models have been extensively applied only to test sites with shallow soil depths.This study addresses this limitation by showing the results of an application to a section of Wanzhou County(Three Gorges Reservoir Area,China),where soil thickness varies from 0 to40 m.Two different approaches were used to derive soil thickness maps:a modified version of the geomorphologically indexed soil thickness(GIST)model,purposely customized to better account for the peculiar setting of the test site,and a regression performed with a machine learning algorithm,i.e.,the random forest,combined with the geomorphological parameters of GIST(GIST-RF).Additionally,the errors of the two models were quantified,and validation with geophysical data was carried out.The results showed that the GIST model could not fully contend with the high spatial variability of soil thickness in the study area:the mean absolute error was 10.68 m with the root-mean-square error(RMSE)of 12.61 m,and the frequency distribution residuals showed a tendency toward underestimation.In contrast,GIST-RF returned a better performance with the mean absolute error of 3.52 m and RMSE of 4.56 m.The derived soil thickness map could be considered a critical fundamental input parameter for further analyses.展开更多
Wood-based panels containing urea-formaldehyde resin result in the long-term release of formaldehyde and threaten human health.In this study,inorganic aluminosilicate coatings prepared by combining metakaolin,silica f...Wood-based panels containing urea-formaldehyde resin result in the long-term release of formaldehyde and threaten human health.In this study,inorganic aluminosilicate coatings prepared by combining metakaolin,silica fume,NaOH,and H_(2)O were applied to the surfaces of wood-based panels to obstruct formaldehyde release.The Si/Al,Na/Al,and H_(2)O/Na_(2)O molar ratios of the coatings were regulated to investigate their effects on the structure and formaldehyde-resistant barrier properties of coatings.Results showed that the cracks in the coatings gradually disappeared and the formaldehyde resistance rates of the barrier increased as the Si/Al molar ratio was increased from 1.6 to 2.2.This value also increased as the Na/Al molar ratio was increased from 0.9 to 1.2 because of the improvement of the degree of polymerization.As the H_(2)O/Na_(2)O molar ratio was increased from 12 to 15,the thickness of the dry film decreased gradually and led to the reduction in the formaldehyde resistance rate.When the Si/Al,Na/Al,and H_(2)O/Na_(2)O molar ratios were 2.2,1.2,and 12,respectively,the inorganic aluminosilicate coating showed good performance as a formaldehyde-resistant barrier and its formaldehyde resistance rate could reach up to 83.2%.展开更多
The safe operating voltage and low volume variation of Li_(3)VO_(4)(LVO)make it an ideal anode material for lithium(Li)-ion batteries.However,the insufficient understanding of the inner storage mechanism hinders the d...The safe operating voltage and low volume variation of Li_(3)VO_(4)(LVO)make it an ideal anode material for lithium(Li)-ion batteries.However,the insufficient understanding of the inner storage mechanism hinders the design of LVO-based electrodes.Herein,we investigate,for the first time,the Li-ion storage activity in LVO via Cl doping.Moreover,N-doped C coating was simultaneously achieved in the Cl doping process,resulting in synergistically improved reaction kinetics.As a result,the as-prepared Cl-doped Li_(3)VO_(4) coated with N-doped C(Cl-LVO@NC)electrodes deliver a discharge capacity of 884.1 mAh/g after 200 cycles at 0.2 A/g,which is the highest among all of the LVO-based electrodes.The Cl-LVO@NC electrodes also exhibit high-capacity retention of 331.1 mAh/g at 8.0 A/g and full capacity recovery after 5 periods of rate testing over 400 cycles.After 5000 cycles at 4.0 A/g,the discharge capacity can be maintained at 423.2 mAh/g,which is superior to most LVO-based electrodes.The Li-ion storage activity in LVO via Cl doping and significant improvement in the high-rate Li-ion storage reported in this work can be used as references for the design of advanced LVO-based electrodes for high-power applications.展开更多
BACKGROUND Recurrence remains the leading cause of poor prognosis in hepatocellular carcinoma(HCC),particularly among patients infected with hepatitis B virus(HBV).The telomerase reverse transcriptase(TERT)promoter is...BACKGROUND Recurrence remains the leading cause of poor prognosis in hepatocellular carcinoma(HCC),particularly among patients infected with hepatitis B virus(HBV).The telomerase reverse transcriptase(TERT)promoter is the most frequently mutated site in HBV-related HCC;however,its prognostic significance is not fully established.AIM To evaluate the prognostic impact of TERT promoter mutations and efficiency of digital polymerase chain reaction(dPCR).METHODS A total of 66 HBV-related HCC patients who underwent hepatectomy were enrolled in this study.DNA extracted from fresh tumor tissues was analyzed for TERT promoter mutations using Sanger sequencing and dPCR.The dPCR assay was optimized by adding 7-deaza-dGTP,CviQ1,and ethylenediaminetetraacetic acid to improve detection sensitivity.Concordance between methods was assessed,and nomogram survival prediction models were developed to evaluate prognostic value based on mutation status.RESULTS TERT promoter mutations were detected in 26/66(39.39%)cases by Sanger sequencing and 30/66(45.45%)by dPCR.The two methods showed high concordance(93.939%,κ=0.876),with dPCR demonstrating 100%sensitivity and 90%specificity.Patients harboring TERT promoter mutations exhibited reduced overall survival and higher recurrence risk.Nomogram models successfully distinguished mutant from non-mutant cases for both overall survival(C-index:0.7651)and disease-free survival(C-index:0.6899).CONCLUSION TERT promoter mutation predicts poor prognosis in HBV-related HCC and serves as a biomarker for risk stratification.Optimized dPCR outperforms Sanger sequencing,and nomograms with TERT status guide precision therapy.展开更多
Electrochemical nitrate(NO_(3)^(-))reduction reaction(eNO_(3)RR)presents a promising and sustainable strategy for converting environmentally hazardous NO_(3)^(-)into value-added ammonia(NH3),thereby addressing both po...Electrochemical nitrate(NO_(3)^(-))reduction reaction(eNO_(3)RR)presents a promising and sustainable strategy for converting environmentally hazardous NO_(3)^(-)into value-added ammonia(NH3),thereby addressing both pollution mitigation and nitrogen resource recovery.However,its practical implementation remains hindered by the limited availability of electrocatalysts that simultaneously offer high activity,selectivity,and stability.In this study,we systematically investigate the catalytic potential of 300 Cu-based heteronuclear trimetallic dual-atom alloys(DAAs),featuring heterometallic dual-atom active sites embedded on a catalytically suboptimal Cu(111)surface.By combining high-throughput firstprinciples calculations with a hierarchical four-step screening strategy,we efficiently identify promising DAA catalysts for eNO_(3)RR.Among them,CrRh and MnRh catalysts stand out,exhibiting ultralow limiting potentials of-0.16 and-0.25 V,respectively,along with excellent selectivity and stability.Constantpotential simulations further reveal that both catalysts maintain high activity and selectivity across varying pH conditions and applied potentials.Geometric and electronic structure analyses indicate that the incorporation of dual-atom sites induces local lattice distortion and charge polarization,effectively tuning the electronic structure of the active centers.Notably,strong d-d orbital interactions between the embedded metal dimers give rise to new molecule-like electronic states near the Fermi level.These states facilitate effective activation of NO_(3)^(-)and NO via an electron“acceptance-donation”mechanism,driven by p-d orbital interactions between adsorbates and metal dimers.This work not only strategically designs efficient heteronuclear Cu-based DAA catalysts but also provides valuable insights into the electronic synergistic effects of dual-atom sites in modulating eNO_(3)RR performance.It establishes a promising platform for demonstrating the feasibility of constructing dual-atom active centers on pure metal surfaces for eNO_(3)RR,while broadening the potential applications of DAAs in electrocatalysis and related fields.展开更多
The following fractional Klein-Gordon-Maxwell system is studied<br /> <p> <img src="Edit_d0190fe4-48ad-4118-8c6c-c585ba971681.bmp" alt="" /> <br /> (-Δ)<sup><em>...The following fractional Klein-Gordon-Maxwell system is studied<br /> <p> <img src="Edit_d0190fe4-48ad-4118-8c6c-c585ba971681.bmp" alt="" /> <br /> (-Δ)<sup><em>p</em></sup> stands for the fractional Laplacian, <em>ω</em> > 0 is a constant, <em>V</em> is vanishing potential and <em>K</em> is a smooth function. Under some suitable conditions on <em>K</em> and <em>f</em>, we obtain a Palais-Smale sequence by using a weaker Ambrosetti-Rabinowitz condition and prove the ground state solution for this system by employing variational methods. In particular, this kind of problem is a vast range of applications and challenges. </p>展开更多
In this paper, we consider the following fourth-order equation of Kirchhoff type<br /> <p> <img src="Edit_bcc9844d-7cbc-494d-90c4-d75364de5658.bmp" alt="" /> </p> <p> ...In this paper, we consider the following fourth-order equation of Kirchhoff type<br /> <p> <img src="Edit_bcc9844d-7cbc-494d-90c4-d75364de5658.bmp" alt="" /> </p> <p> where <i>a</i>, <i>b</i> > 0 are constants, 3 < <i>p</i> < 5, <i>V</i> ∈ <i>C</i> (R<sup>3</sup>, R);Δ<sup>2</sup>: = Δ (Δ) is the biharmonic operator. By using Symmetric Mountain Pass Theorem and variational methods, we prove that the above equation admits infinitely many high energy solutions under some sufficient assumptions on <i>V</i> (<i>x</i>). We make some assumptions on the potential <i>V</i> (<i>x</i>) to solve the difficulty of lack of compactness of the Sobolev embedding. Our results improve some related results in the literature. </p>展开更多
This paper is concerned with the following variable-order fractional Laplacian equations , where N ≥ 1 and N > 2s(x,y) for (x,y) ∈ Ω × Ω, Ω is a bounded domain in R<sup>N</sup>, s(⋅)...This paper is concerned with the following variable-order fractional Laplacian equations , where N ≥ 1 and N > 2s(x,y) for (x,y) ∈ Ω × Ω, Ω is a bounded domain in R<sup>N</sup>, s(⋅) ∈ C (R<sup>N</sup> × R<sup>N</sup>, (0,1)), (-Δ)<sup>s(⋅)</sup> is the variable-order fractional Laplacian operator, λ, μ > 0 are two parameters, V: Ω → [0, ∞) is a continuous function, f ∈ C(Ω × R) and q ∈ C(Ω). Under some suitable conditions on f, we obtain two solutions for this problem by employing the mountain pass theorem and Ekeland’s variational principle. Our result generalizes the related ones in the literature.展开更多
Landslide susceptibility mapping is vital for landslide risk management and urban planning.In this study,we used three statistical models[frequency ratio,certainty factor and index of entropy(IOE)]and a machine learni...Landslide susceptibility mapping is vital for landslide risk management and urban planning.In this study,we used three statistical models[frequency ratio,certainty factor and index of entropy(IOE)]and a machine learning model[random forest(RF)]for landslide susceptibility mapping in Wanzhou County,China.First,a landslide inventory map was prepared using earlier geotechnical investigation reports,aerial images,and field surveys.Then,the redundant factors were excluded from the initial fourteen landslide causal factors via factor correlation analysis.To determine the most effective causal factors,landslide susceptibility evaluations were performed based on four cases with different combinations of factors("cases").In the analysis,465(70%)landslide locations were randomly selected for model training,and 200(30%)landslide locations were selected for verification.The results showed that case 3 produced the best performance for the statistical models and that case 2 produced the best performance for the RF model.Finally,the receiver operating characteristic(ROC)curve was used to verify the accuracy of each model's results for its respective optimal case.The ROC curve analysis showed that the machine learning model performed better than the other three models,and among the three statistical models,the IOE model with weight coefficients was superior.展开更多
We investigated the adsorption performance of five Fe-based MOFs(Fe-BTC,MIL-100(Fe),MIL-101(Fe),MIL-53(Fe)and MIL-88 C(Fe))for removal of antimonite(Sb(Ⅲ))and antimonate(Sb(Ⅴ))from water.Among these MOFs,MIL-101(Fe)...We investigated the adsorption performance of five Fe-based MOFs(Fe-BTC,MIL-100(Fe),MIL-101(Fe),MIL-53(Fe)and MIL-88 C(Fe))for removal of antimonite(Sb(Ⅲ))and antimonate(Sb(Ⅴ))from water.Among these MOFs,MIL-101(Fe)exhibited the best adsorption capacities for both Sb(Ⅲ)and Sb(Ⅴ)(151.8 and 472.8 mg/g,respectively)which were higher than those of most adsorbents previously reported.The effect of steric hindrance was evident during Sb removal using the Fe-based MOFs,and the proper diameter of the smallest cage windows/channels should be considered an important parameter during the evaluation and selection of MOFs.Additionally,the adsorption capacities of MIL-101(Fe)for Sb(Ⅴ)decreased with increasing initial p H values(from 3.0 to 8.0),while the opposite trend was observed for Sb(Ⅲ).Chloride,nitrate and sulfate ions had a negligible influence on Sb(Ⅴ)adsorption,while NO3-and SO42-improved Sb(Ⅲ)adsorption.This result implies that inner sphere complexes might form during both Sb(Ⅲ)and Sb(Ⅴ)adsorption.展开更多
BACKGROUND： Hypothalamus-pituitary-adrenal （HPA） axis dysfunction has been closely linked to anxiety. Previous studies have shown that Valeriana jatamansi Jones extract exhibits clear anxiolytic effects, but it is ...BACKGROUND： Hypothalamus-pituitary-adrenal （HPA） axis dysfunction has been closely linked to anxiety. Previous studies have shown that Valeriana jatamansi Jones extract exhibits clear anxiolytic effects, but it is unclear about the mechanism underlying regulation of the HPA axis dysfunction in these anxiolytic effects. OBJECTIVE： To observe the effects of Valeriana jatamansi Jones （Zhizhu Xiang） extract on HPA axis function in a rat model of anxiety, and to compare these effects with positive control estazolam. DESIGN, TIME AND SETTING： Randomized, controlled, animal experiment was performed at Chengdu University of Traditional Chinese Medicine, China, between February and September in 2006. MATERIALS： Estazolam was purchased from Shanghai Jiufu Pharmaceutical, China; Valeriana jatamansiJones was purchased from the Lotus Pond Market for Chinese Herbal Medicine in Chengdu. It consisted of iridoids and flavonoid components. METHODS： A total of 72 Sprague Dawley rats, aged 2 months, were randomly assigned to 6 groups low-, medium-, and high-dose Valerianajatamansi Jones groups intragastrically injected with 0.3, 0.6, and 0.9 g/kg per day Valerianajatamansi Jones extract, respectively; estazolam group intragastrically injected with 1.5 mg/kg per day estazolam; model and normal groups administered 5 mL physiological saline. Anxiety was established in all groups, except the normal group, through the use of elevated plus-maze test at 7 days following drug administration. MAIN OUTCOME MEASURES： Blood β-endorphin and corticosterone levels were determined using enzyme-linked immunosorbent assay following treatment with ValerianajatamansiJones extract. Expressions of HPA axis-related genes were measured by cDNA microarray. RESULTS： Blood β-endorphin and corticosterone levels were significantly greater in the model group than in the normal group. Compared with the model group, levels decreased with Valeriana jatamansi Jones extract or estazolam treatment, particularly in the low-dose Valeriana jatamansi Jones group （P〈 0.01）. cDNA microarray results demonstrated that corticotropin-releasing hormone and Orexin, which are associated with HPA axis function, were differentially expressed; expression increased in the model group, but decreased in rats treated with Valerianajatamansi Jones extract. CONCLUSION： Valerianajatamansi Jones extract plays a role in regulating HPA axis function in a rat model of anxiety, and this effect was superior to estazolam.展开更多
Stromal cell-derived factor-1 and its receptor CXCR4 are essential regulators of the neurogenesis that occurs in the adult hippocampal dentate gyrus.However,the effects of CXCR7,a new atypical receptor of stromal cell...Stromal cell-derived factor-1 and its receptor CXCR4 are essential regulators of the neurogenesis that occurs in the adult hippocampal dentate gyrus.However,the effects of CXCR7,a new atypical receptor of stromal cell-derived factor-1,on hippocampal neurogenesis after a stroke remain largely unknown.Our study is the first to investigate the effect of a CXCR7-neutralizing antibody on neurogenesis in the dentate gyrus and the associated recovery of cognitive function of rats in the chronic stage of cerebral ischemia.The rats were randomly divided into sham,sham+anti-CXCR7,ischemia and ischemia+anti-CXCR7 groups.Endothelin-1 was injected in the ipsilateral motor cortex and striatum to induce focal cerebral ischemia.Sham group rats were injected with saline instead of endothelin-1 via intracranial injection.Both sham and ischemic rats were treated with intraventricular infusions of CXCR7-neutralizing antibodies for 6 days 1 week after surgery.Immunofluorescence staining with doublecortin,a marker for neuronal precursors,was performed to assess the neurogenesis in the dentate gyrus.We found that anti-CXCR7 antibody infusion enhanced the proliferation and dendritic development of doublecortin-labeled cells in the dentate gyrus in both ischemic and sham-operated rats.Spatial learning and memory functions were assessed by Morris water maze tests 30-32 days after ischemia.CXCR7-neutralizing antibody treatment significantly reduced the escape latency of the spatial navigation trial and increased the time spent in the target quadrant of spatial probe trial in animals that received ischemic insult,but not in sham operated rats.These results suggest that CXCR7-neutralizing antibody enhances the neurogenesis in the dentate gyrus and improves the cognitive function after cerebral ischemia in rats.All animal experimental protocols and procedures were approved by the Institutional Animal Care and Use Committee of China Medical University(CMU16089 R)on December 8,2016.展开更多
BACKGROUND As a critical early event in hepatocellular carcinogenesis,telomerase activation might be a promising and critical biomarker for hepatocellular carcinoma(HCC)patients,and its function in the genesis and tre...BACKGROUND As a critical early event in hepatocellular carcinogenesis,telomerase activation might be a promising and critical biomarker for hepatocellular carcinoma(HCC)patients,and its function in the genesis and treatment of HCC has gained much attention over the past two decades.AIM To perform a bibliometric analysis to systematically assess the current state of research on HCC-related telomerase.METHODS The Web of Science Core Collection and PubMed were systematically searched to retrieve publications pertaining to HCC/telomerase limited to“articles”and“reviews”published in English.A total of 873 relevant publications related to HCC and telomerase were identified.We employed the Bibliometrix package in R to extract and analyze the fundamental information of the publications,such as the trends in the publications,citation counts,most prolific or influential writers,and most popular journals;to screen for keywords occurring at high frequency;and to draw collaboration and cluster analysis charts on the basis of coauthorship and co-occurrences.VOSviewer was utilized to compile and visualize the bibliometric data.RESULTS A surge of 51 publications on HCC/telomerase research occurred in 2016,the most productive year from 1996 to 2023,accompanied by the peak citation count recorded in 2016.Up to December 2023,35226 citations were made to all publications,an average of 46.6 citations to each paper.The United States received the most citations(n=13531),followed by China(n=7427)and Japan(n=5754).In terms of national cooperation,China presented the highest centrality,its strongest bonds being to the United States and Japan.Among the 20 academic institutions with the most publications,ten came from China and the rest of Asia,though the University of Paris Cité,Public Assistance-Hospitals of Paris,and the National Institute of Health and Medical Research(INSERM)were the most prolific.As for individual contributions,Hisatomi H,Kaneko S,and Ide T were the three most prolific authors.Kaneko S ranked first by H-index,G-index,and overall publication count,while Zucman-Rossi J ranked first in citation count.The five most popular journals were the World Journal of Gastroenterology,Hepatology,Journal of Hepatology,Oncotarget,and Oncogene,while Nature Genetics,Hepatology,and Nature Reviews Disease Primers had the most citations.We extracted 2293 keywords from the publications,120 of which appeared more than ten times.The most frequent were HCC,telomerase and human telomerase reverse transcriptase(hTERT).Keywords such as mutational landscape,TERT promoter mutations,landscape,risk,and prognosis were among the most common issues in this field in the last three years and may be topics for research in the coming years.CONCLUSION Our bibliometric analysis provides a comprehensive overview of HCC/telomerase research and insights into promising upcoming research.展开更多
To improve the corrosion resistance and surface electrical conductivity of AISI 430 stainless steel （430 SS） as bipolar plates for proton exchange membrane fuel cells （PEMFCs） used in marine environment, a tungste...To improve the corrosion resistance and surface electrical conductivity of AISI 430 stainless steel （430 SS） as bipolar plates for proton exchange membrane fuel cells （PEMFCs） used in marine environment, a tungsten alloying layer has been successfully prepared on 430 SS substrate via the plasma surface diffusion alloying technique. The tungsten- modified （W-modified） 430 SS displays a 7-8 Ixm tungsten alloying layer with a body-centered-cubic structure. The W-modified surface also shows a better hydrophobicity with contact angle of 93.5~ and a lower interfacial contact resistance compared with the untreated 430 SS. The potentiodynamic and potentiostatic polarization and electrochemical impedance spectroscopy measurements show that the corrosion resistance of 430 SS is obviously improved in simulated PEMFC environment （0.05 M H2SO4 ＋ 2 ppm HF ＋ 0.01 M NaC1 solution at 70℃）, after the plasma surface diffusion alloying process.展开更多
Dysregulation of microRNAs(miRNAs)in adipocytes plays a critical role in the pathogenesis of obesity.However,the signaling mechanisms regulating miRNAs production in adipose tissue remain largely unclear.Here,we show ...Dysregulation of microRNAs(miRNAs)in adipocytes plays a critical role in the pathogenesis of obesity.However,the signaling mechanisms regulating miRNAs production in adipose tissue remain largely unclear.Here,we show that adipose tissue-specific knockout of Ras homolog enriched in brain(Rheb),a direct upstream activator of mTOR,increases miR-182-5p level in mouse subcutaneous white adipose tissues.Interestingly,the inhibition of mTOR signaling by rapamycin has no effect on miR-182-5p level in primary subcutaneous white adipocytes,suggesting the presence of a mTOR-independent mechanism regulating Rheb-mediated miR-182-5p expression.Consistent with this view,Rheb-ablation activates the cAMP/PPARγsignaling pathway.In addition,treatment of white adipocytes with pioglitazone,a PPARγagonist,dramatically upregulates miR-182-5p levels.Our study reveals a unique mechanism by which Rheb regulates miR-182-5p in adipocytes.Given that increasing miR-182-5p in adipose tissue promotes beige fat development,our study also suggests a unique mechanism by which Rheb promotes thermogenesis and energy expenditure.展开更多
基金support for this work:National Natural Science Foundation of China(Grant Nos.41877525,61971037 and 31727901)Chongqing Key Laboratory of Geological Environment Monitoring and Disaster Early-warning in Three Gorges Reservoir Area(No.MP2020B0301)。
文摘Soil thickness,intended as depth to bedrock,is a key input parameter for many environmental models.Nevertheless,it is often difficult to obtain a reliable spatially exhaustive soil thickness map in widearea applications,and existing prediction models have been extensively applied only to test sites with shallow soil depths.This study addresses this limitation by showing the results of an application to a section of Wanzhou County(Three Gorges Reservoir Area,China),where soil thickness varies from 0 to40 m.Two different approaches were used to derive soil thickness maps:a modified version of the geomorphologically indexed soil thickness(GIST)model,purposely customized to better account for the peculiar setting of the test site,and a regression performed with a machine learning algorithm,i.e.,the random forest,combined with the geomorphological parameters of GIST(GIST-RF).Additionally,the errors of the two models were quantified,and validation with geophysical data was carried out.The results showed that the GIST model could not fully contend with the high spatial variability of soil thickness in the study area:the mean absolute error was 10.68 m with the root-mean-square error(RMSE)of 12.61 m,and the frequency distribution residuals showed a tendency toward underestimation.In contrast,GIST-RF returned a better performance with the mean absolute error of 3.52 m and RMSE of 4.56 m.The derived soil thickness map could be considered a critical fundamental input parameter for further analyses.
基金financially supported by the National Key Research and Development Program of China(Nos.2016 YFC0700607,2017YFB0304305,and 2016YFC0700901)the Fundamental Research Funds for the Central Universities of China(No.FRF-TP-20-006A3).
文摘Wood-based panels containing urea-formaldehyde resin result in the long-term release of formaldehyde and threaten human health.In this study,inorganic aluminosilicate coatings prepared by combining metakaolin,silica fume,NaOH,and H_(2)O were applied to the surfaces of wood-based panels to obstruct formaldehyde release.The Si/Al,Na/Al,and H_(2)O/Na_(2)O molar ratios of the coatings were regulated to investigate their effects on the structure and formaldehyde-resistant barrier properties of coatings.Results showed that the cracks in the coatings gradually disappeared and the formaldehyde resistance rates of the barrier increased as the Si/Al molar ratio was increased from 1.6 to 2.2.This value also increased as the Na/Al molar ratio was increased from 0.9 to 1.2 because of the improvement of the degree of polymerization.As the H_(2)O/Na_(2)O molar ratio was increased from 12 to 15,the thickness of the dry film decreased gradually and led to the reduction in the formaldehyde resistance rate.When the Si/Al,Na/Al,and H_(2)O/Na_(2)O molar ratios were 2.2,1.2,and 12,respectively,the inorganic aluminosilicate coating showed good performance as a formaldehyde-resistant barrier and its formaldehyde resistance rate could reach up to 83.2%.
基金supported by the National Natural Science Foundation of China(No.52101262)Distinguished Youth Foundation of Hubei Province(2019CFA084)+1 种基金Educational offi ce of Hubei Province(Q20201201)the 111 project(D20015).
文摘The safe operating voltage and low volume variation of Li_(3)VO_(4)(LVO)make it an ideal anode material for lithium(Li)-ion batteries.However,the insufficient understanding of the inner storage mechanism hinders the design of LVO-based electrodes.Herein,we investigate,for the first time,the Li-ion storage activity in LVO via Cl doping.Moreover,N-doped C coating was simultaneously achieved in the Cl doping process,resulting in synergistically improved reaction kinetics.As a result,the as-prepared Cl-doped Li_(3)VO_(4) coated with N-doped C(Cl-LVO@NC)electrodes deliver a discharge capacity of 884.1 mAh/g after 200 cycles at 0.2 A/g,which is the highest among all of the LVO-based electrodes.The Cl-LVO@NC electrodes also exhibit high-capacity retention of 331.1 mAh/g at 8.0 A/g and full capacity recovery after 5 periods of rate testing over 400 cycles.After 5000 cycles at 4.0 A/g,the discharge capacity can be maintained at 423.2 mAh/g,which is superior to most LVO-based electrodes.The Li-ion storage activity in LVO via Cl doping and significant improvement in the high-rate Li-ion storage reported in this work can be used as references for the design of advanced LVO-based electrodes for high-power applications.
基金Supported by National Key Research and Development Program of China,No.2023YFF0613304Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences,No.2023-I2M-2-004,No.2024-I2M-C&T-B-069,and No.2025-I2M-C&T-B-057.
文摘BACKGROUND Recurrence remains the leading cause of poor prognosis in hepatocellular carcinoma(HCC),particularly among patients infected with hepatitis B virus(HBV).The telomerase reverse transcriptase(TERT)promoter is the most frequently mutated site in HBV-related HCC;however,its prognostic significance is not fully established.AIM To evaluate the prognostic impact of TERT promoter mutations and efficiency of digital polymerase chain reaction(dPCR).METHODS A total of 66 HBV-related HCC patients who underwent hepatectomy were enrolled in this study.DNA extracted from fresh tumor tissues was analyzed for TERT promoter mutations using Sanger sequencing and dPCR.The dPCR assay was optimized by adding 7-deaza-dGTP,CviQ1,and ethylenediaminetetraacetic acid to improve detection sensitivity.Concordance between methods was assessed,and nomogram survival prediction models were developed to evaluate prognostic value based on mutation status.RESULTS TERT promoter mutations were detected in 26/66(39.39%)cases by Sanger sequencing and 30/66(45.45%)by dPCR.The two methods showed high concordance(93.939%,κ=0.876),with dPCR demonstrating 100%sensitivity and 90%specificity.Patients harboring TERT promoter mutations exhibited reduced overall survival and higher recurrence risk.Nomogram models successfully distinguished mutant from non-mutant cases for both overall survival(C-index:0.7651)and disease-free survival(C-index:0.6899).CONCLUSION TERT promoter mutation predicts poor prognosis in HBV-related HCC and serves as a biomarker for risk stratification.Optimized dPCR outperforms Sanger sequencing,and nomograms with TERT status guide precision therapy.
基金supported by the Water Conservancy Science and Technology Project of Jiangsu Province,China(No.2024005,2025012)。
文摘Electrochemical nitrate(NO_(3)^(-))reduction reaction(eNO_(3)RR)presents a promising and sustainable strategy for converting environmentally hazardous NO_(3)^(-)into value-added ammonia(NH3),thereby addressing both pollution mitigation and nitrogen resource recovery.However,its practical implementation remains hindered by the limited availability of electrocatalysts that simultaneously offer high activity,selectivity,and stability.In this study,we systematically investigate the catalytic potential of 300 Cu-based heteronuclear trimetallic dual-atom alloys(DAAs),featuring heterometallic dual-atom active sites embedded on a catalytically suboptimal Cu(111)surface.By combining high-throughput firstprinciples calculations with a hierarchical four-step screening strategy,we efficiently identify promising DAA catalysts for eNO_(3)RR.Among them,CrRh and MnRh catalysts stand out,exhibiting ultralow limiting potentials of-0.16 and-0.25 V,respectively,along with excellent selectivity and stability.Constantpotential simulations further reveal that both catalysts maintain high activity and selectivity across varying pH conditions and applied potentials.Geometric and electronic structure analyses indicate that the incorporation of dual-atom sites induces local lattice distortion and charge polarization,effectively tuning the electronic structure of the active centers.Notably,strong d-d orbital interactions between the embedded metal dimers give rise to new molecule-like electronic states near the Fermi level.These states facilitate effective activation of NO_(3)^(-)and NO via an electron“acceptance-donation”mechanism,driven by p-d orbital interactions between adsorbates and metal dimers.This work not only strategically designs efficient heteronuclear Cu-based DAA catalysts but also provides valuable insights into the electronic synergistic effects of dual-atom sites in modulating eNO_(3)RR performance.It establishes a promising platform for demonstrating the feasibility of constructing dual-atom active centers on pure metal surfaces for eNO_(3)RR,while broadening the potential applications of DAAs in electrocatalysis and related fields.
文摘The following fractional Klein-Gordon-Maxwell system is studied<br /> <p> <img src="Edit_d0190fe4-48ad-4118-8c6c-c585ba971681.bmp" alt="" /> <br /> (-Δ)<sup><em>p</em></sup> stands for the fractional Laplacian, <em>ω</em> > 0 is a constant, <em>V</em> is vanishing potential and <em>K</em> is a smooth function. Under some suitable conditions on <em>K</em> and <em>f</em>, we obtain a Palais-Smale sequence by using a weaker Ambrosetti-Rabinowitz condition and prove the ground state solution for this system by employing variational methods. In particular, this kind of problem is a vast range of applications and challenges. </p>
文摘In this paper, we consider the following fourth-order equation of Kirchhoff type<br /> <p> <img src="Edit_bcc9844d-7cbc-494d-90c4-d75364de5658.bmp" alt="" /> </p> <p> where <i>a</i>, <i>b</i> > 0 are constants, 3 < <i>p</i> < 5, <i>V</i> ∈ <i>C</i> (R<sup>3</sup>, R);Δ<sup>2</sup>: = Δ (Δ) is the biharmonic operator. By using Symmetric Mountain Pass Theorem and variational methods, we prove that the above equation admits infinitely many high energy solutions under some sufficient assumptions on <i>V</i> (<i>x</i>). We make some assumptions on the potential <i>V</i> (<i>x</i>) to solve the difficulty of lack of compactness of the Sobolev embedding. Our results improve some related results in the literature. </p>
文摘This paper is concerned with the following variable-order fractional Laplacian equations , where N ≥ 1 and N > 2s(x,y) for (x,y) ∈ Ω × Ω, Ω is a bounded domain in R<sup>N</sup>, s(⋅) ∈ C (R<sup>N</sup> × R<sup>N</sup>, (0,1)), (-Δ)<sup>s(⋅)</sup> is the variable-order fractional Laplacian operator, λ, μ > 0 are two parameters, V: Ω → [0, ∞) is a continuous function, f ∈ C(Ω × R) and q ∈ C(Ω). Under some suitable conditions on f, we obtain two solutions for this problem by employing the mountain pass theorem and Ekeland’s variational principle. Our result generalizes the related ones in the literature.
基金the projects ‘‘The risk assessment of geological hazards induced by reservoir water level fluctuation in Chongqing, Three-Gorges Reservoir, China.’’ (No. 2016065135)‘‘The study of mechanism and forecast criterion of the gentle-dip landslides in The Three Gorges Reservoir Region, China’’ (No. 41572292) funded by the National Natural Science Foundation of China
文摘Landslide susceptibility mapping is vital for landslide risk management and urban planning.In this study,we used three statistical models[frequency ratio,certainty factor and index of entropy(IOE)]and a machine learning model[random forest(RF)]for landslide susceptibility mapping in Wanzhou County,China.First,a landslide inventory map was prepared using earlier geotechnical investigation reports,aerial images,and field surveys.Then,the redundant factors were excluded from the initial fourteen landslide causal factors via factor correlation analysis.To determine the most effective causal factors,landslide susceptibility evaluations were performed based on four cases with different combinations of factors("cases").In the analysis,465(70%)landslide locations were randomly selected for model training,and 200(30%)landslide locations were selected for verification.The results showed that case 3 produced the best performance for the statistical models and that case 2 produced the best performance for the RF model.Finally,the receiver operating characteristic(ROC)curve was used to verify the accuracy of each model's results for its respective optimal case.The ROC curve analysis showed that the machine learning model performed better than the other three models,and among the three statistical models,the IOE model with weight coefficients was superior.
基金supported by the National Natural Science Foundation of China (No. 41201302)the Natural Science Foundation of Shanghai (No. 17ZR1407000)the Fundamental Research Funds for the Central Universities (No. 222201514337)
文摘We investigated the adsorption performance of five Fe-based MOFs(Fe-BTC,MIL-100(Fe),MIL-101(Fe),MIL-53(Fe)and MIL-88 C(Fe))for removal of antimonite(Sb(Ⅲ))and antimonate(Sb(Ⅴ))from water.Among these MOFs,MIL-101(Fe)exhibited the best adsorption capacities for both Sb(Ⅲ)and Sb(Ⅴ)(151.8 and 472.8 mg/g,respectively)which were higher than those of most adsorbents previously reported.The effect of steric hindrance was evident during Sb removal using the Fe-based MOFs,and the proper diameter of the smallest cage windows/channels should be considered an important parameter during the evaluation and selection of MOFs.Additionally,the adsorption capacities of MIL-101(Fe)for Sb(Ⅴ)decreased with increasing initial p H values(from 3.0 to 8.0),while the opposite trend was observed for Sb(Ⅲ).Chloride,nitrate and sulfate ions had a negligible influence on Sb(Ⅴ)adsorption,while NO3-and SO42-improved Sb(Ⅲ)adsorption.This result implies that inner sphere complexes might form during both Sb(Ⅲ)and Sb(Ⅴ)adsorption.
基金Project of Sichuan Provincial Traditional Chinese Medicine Administration,No.200674Science Foundation of Southwest Jiaotong University,No.2006A10+1 种基金"Key New Drug Innovation" National Science and Technology Major Projects During Eleventh Five-Year Plan,No.2009ZX09103-370Chengdu Science and Technology Major Projects During Eleventh Five-Year Plan,No.09GGZD060SF-012
文摘BACKGROUND： Hypothalamus-pituitary-adrenal （HPA） axis dysfunction has been closely linked to anxiety. Previous studies have shown that Valeriana jatamansi Jones extract exhibits clear anxiolytic effects, but it is unclear about the mechanism underlying regulation of the HPA axis dysfunction in these anxiolytic effects. OBJECTIVE： To observe the effects of Valeriana jatamansi Jones （Zhizhu Xiang） extract on HPA axis function in a rat model of anxiety, and to compare these effects with positive control estazolam. DESIGN, TIME AND SETTING： Randomized, controlled, animal experiment was performed at Chengdu University of Traditional Chinese Medicine, China, between February and September in 2006. MATERIALS： Estazolam was purchased from Shanghai Jiufu Pharmaceutical, China; Valeriana jatamansiJones was purchased from the Lotus Pond Market for Chinese Herbal Medicine in Chengdu. It consisted of iridoids and flavonoid components. METHODS： A total of 72 Sprague Dawley rats, aged 2 months, were randomly assigned to 6 groups low-, medium-, and high-dose Valerianajatamansi Jones groups intragastrically injected with 0.3, 0.6, and 0.9 g/kg per day Valerianajatamansi Jones extract, respectively; estazolam group intragastrically injected with 1.5 mg/kg per day estazolam; model and normal groups administered 5 mL physiological saline. Anxiety was established in all groups, except the normal group, through the use of elevated plus-maze test at 7 days following drug administration. MAIN OUTCOME MEASURES： Blood β-endorphin and corticosterone levels were determined using enzyme-linked immunosorbent assay following treatment with ValerianajatamansiJones extract. Expressions of HPA axis-related genes were measured by cDNA microarray. RESULTS： Blood β-endorphin and corticosterone levels were significantly greater in the model group than in the normal group. Compared with the model group, levels decreased with Valeriana jatamansi Jones extract or estazolam treatment, particularly in the low-dose Valeriana jatamansi Jones group （P〈 0.01）. cDNA microarray results demonstrated that corticotropin-releasing hormone and Orexin, which are associated with HPA axis function, were differentially expressed; expression increased in the model group, but decreased in rats treated with Valerianajatamansi Jones extract. CONCLUSION： Valerianajatamansi Jones extract plays a role in regulating HPA axis function in a rat model of anxiety, and this effect was superior to estazolam.
基金supported by the National Natural Science Foundation of China,No.81401002(to SSZ)
文摘Stromal cell-derived factor-1 and its receptor CXCR4 are essential regulators of the neurogenesis that occurs in the adult hippocampal dentate gyrus.However,the effects of CXCR7,a new atypical receptor of stromal cell-derived factor-1,on hippocampal neurogenesis after a stroke remain largely unknown.Our study is the first to investigate the effect of a CXCR7-neutralizing antibody on neurogenesis in the dentate gyrus and the associated recovery of cognitive function of rats in the chronic stage of cerebral ischemia.The rats were randomly divided into sham,sham+anti-CXCR7,ischemia and ischemia+anti-CXCR7 groups.Endothelin-1 was injected in the ipsilateral motor cortex and striatum to induce focal cerebral ischemia.Sham group rats were injected with saline instead of endothelin-1 via intracranial injection.Both sham and ischemic rats were treated with intraventricular infusions of CXCR7-neutralizing antibodies for 6 days 1 week after surgery.Immunofluorescence staining with doublecortin,a marker for neuronal precursors,was performed to assess the neurogenesis in the dentate gyrus.We found that anti-CXCR7 antibody infusion enhanced the proliferation and dendritic development of doublecortin-labeled cells in the dentate gyrus in both ischemic and sham-operated rats.Spatial learning and memory functions were assessed by Morris water maze tests 30-32 days after ischemia.CXCR7-neutralizing antibody treatment significantly reduced the escape latency of the spatial navigation trial and increased the time spent in the target quadrant of spatial probe trial in animals that received ischemic insult,but not in sham operated rats.These results suggest that CXCR7-neutralizing antibody enhances the neurogenesis in the dentate gyrus and improves the cognitive function after cerebral ischemia in rats.All animal experimental protocols and procedures were approved by the Institutional Animal Care and Use Committee of China Medical University(CMU16089 R)on December 8,2016.
基金the Beijing Hope Run Special Fund of Cancer Foundation of China,No.LC2020L05.
文摘BACKGROUND As a critical early event in hepatocellular carcinogenesis,telomerase activation might be a promising and critical biomarker for hepatocellular carcinoma(HCC)patients,and its function in the genesis and treatment of HCC has gained much attention over the past two decades.AIM To perform a bibliometric analysis to systematically assess the current state of research on HCC-related telomerase.METHODS The Web of Science Core Collection and PubMed were systematically searched to retrieve publications pertaining to HCC/telomerase limited to“articles”and“reviews”published in English.A total of 873 relevant publications related to HCC and telomerase were identified.We employed the Bibliometrix package in R to extract and analyze the fundamental information of the publications,such as the trends in the publications,citation counts,most prolific or influential writers,and most popular journals;to screen for keywords occurring at high frequency;and to draw collaboration and cluster analysis charts on the basis of coauthorship and co-occurrences.VOSviewer was utilized to compile and visualize the bibliometric data.RESULTS A surge of 51 publications on HCC/telomerase research occurred in 2016,the most productive year from 1996 to 2023,accompanied by the peak citation count recorded in 2016.Up to December 2023,35226 citations were made to all publications,an average of 46.6 citations to each paper.The United States received the most citations(n=13531),followed by China(n=7427)and Japan(n=5754).In terms of national cooperation,China presented the highest centrality,its strongest bonds being to the United States and Japan.Among the 20 academic institutions with the most publications,ten came from China and the rest of Asia,though the University of Paris Cité,Public Assistance-Hospitals of Paris,and the National Institute of Health and Medical Research(INSERM)were the most prolific.As for individual contributions,Hisatomi H,Kaneko S,and Ide T were the three most prolific authors.Kaneko S ranked first by H-index,G-index,and overall publication count,while Zucman-Rossi J ranked first in citation count.The five most popular journals were the World Journal of Gastroenterology,Hepatology,Journal of Hepatology,Oncotarget,and Oncogene,while Nature Genetics,Hepatology,and Nature Reviews Disease Primers had the most citations.We extracted 2293 keywords from the publications,120 of which appeared more than ten times.The most frequent were HCC,telomerase and human telomerase reverse transcriptase(hTERT).Keywords such as mutational landscape,TERT promoter mutations,landscape,risk,and prognosis were among the most common issues in this field in the last three years and may be topics for research in the coming years.CONCLUSION Our bibliometric analysis provides a comprehensive overview of HCC/telomerase research and insights into promising upcoming research.
基金financially supported by the National Natural Science Foundation of China (Nos. 51479019 and 21476035)Fundamental Research Funds for Central Universities (No. 3132014323)
文摘To improve the corrosion resistance and surface electrical conductivity of AISI 430 stainless steel （430 SS） as bipolar plates for proton exchange membrane fuel cells （PEMFCs） used in marine environment, a tungsten alloying layer has been successfully prepared on 430 SS substrate via the plasma surface diffusion alloying technique. The tungsten- modified （W-modified） 430 SS displays a 7-8 Ixm tungsten alloying layer with a body-centered-cubic structure. The W-modified surface also shows a better hydrophobicity with contact angle of 93.5~ and a lower interfacial contact resistance compared with the untreated 430 SS. The potentiodynamic and potentiostatic polarization and electrochemical impedance spectroscopy measurements show that the corrosion resistance of 430 SS is obviously improved in simulated PEMFC environment （0.05 M H2SO4 ＋ 2 ppm HF ＋ 0.01 M NaC1 solution at 70℃）, after the plasma surface diffusion alloying process.
基金supported by grants 82170886 and 81800758 from the National Nature Science Foundation of Chinapartially supported by the Fundamental Research Funds for the Central Universities of Central South University(2021zzts1048)。
文摘Dysregulation of microRNAs(miRNAs)in adipocytes plays a critical role in the pathogenesis of obesity.However,the signaling mechanisms regulating miRNAs production in adipose tissue remain largely unclear.Here,we show that adipose tissue-specific knockout of Ras homolog enriched in brain(Rheb),a direct upstream activator of mTOR,increases miR-182-5p level in mouse subcutaneous white adipose tissues.Interestingly,the inhibition of mTOR signaling by rapamycin has no effect on miR-182-5p level in primary subcutaneous white adipocytes,suggesting the presence of a mTOR-independent mechanism regulating Rheb-mediated miR-182-5p expression.Consistent with this view,Rheb-ablation activates the cAMP/PPARγsignaling pathway.In addition,treatment of white adipocytes with pioglitazone,a PPARγagonist,dramatically upregulates miR-182-5p levels.Our study reveals a unique mechanism by which Rheb regulates miR-182-5p in adipocytes.Given that increasing miR-182-5p in adipose tissue promotes beige fat development,our study also suggests a unique mechanism by which Rheb promotes thermogenesis and energy expenditure.
