Keloid may induce severe impairment of life quality for the patients,although keloid is a cutaneous benign tumor.Collagen triple helix repeat containing protein 1 (Cthrc1) was identified as a novel gene that was origi...Keloid may induce severe impairment of life quality for the patients,although keloid is a cutaneous benign tumor.Collagen triple helix repeat containing protein 1 (Cthrc1) was identified as a novel gene that was originally found in adventitial fibroblasts after arterial injury.To address the role of Cthrcl in keloid,the expression level of Cthrcl was assessed in normal skin and keloid tissue,as well as in normal fibroblasts (NFs)and keloid fibroblasts (KFs)by using quantitative PCR,Western blotting and immunohistochemical analysis.The results showed that Cthrcl was increased in keloid tissue and KFs as compared with normal skin and NFs.Meanwhile,CCK8 and Transwell assays found the cellular proliferation and migration of KFs were increased as compared with NFs.Further,to verify the function of Cthrcl in NFs and K.Fs,we increased Cthrcl expression by transfecting lentivirns (LV) vectors LV-Cthrcl.The cellular proliferation and migration,collagen synthesis and the influence on TGF-β and YAP signaling were tested.The cellular proliferation and migration were increased in NFs-Cthrcl as compared with NFs-control.Meanwhile,YAP expression and nuclear-location was increased in NFs-Cthrcl.On the contrary,when Cthrcl was overexpressed in KFs,the cellular migration was suppressed and YAP expression was reduced and transferred to cytoplasm in KFs-Cthrcl as compared with KFs-control.But the expression level of collagen I was decreased and pSmad2/3 nucleus transfer was suppressed in both NFs-Cthrc1 and KFs-Cthrc1 by using Western blotting and immunofluorescence.Increased Cthrcl activated NFs by promoting YAP nucleus translocation,whereas suppressed KFs by inhibiting YAP nucleus translocation.Enhanced Cthrcl decreased collagen I in both NFs and KFs by inhibiting TGF-β/Smad pathway.In conclusion,Cthrcl may play a role in the pathogenesis of keloid by inhibiting collagen synthesis and fibroblasts migration via suppressing TGF-β/Smad pathway and YAP nucleus translocation.展开更多
Urinary incontinence(UI)is a disease that quietly yet seriously impacts women's health and represents a global health burden that is often neglected.This study aims to systematically assess the prevalence and dyna...Urinary incontinence(UI)is a disease that quietly yet seriously impacts women's health and represents a global health burden that is often neglected.This study aims to systematically assess the prevalence and dynamics of female UI in China,and can inform further policies and have international implications.This study used three nationwide investigations:A national cross-sectional survey in 2021;another nationwide cross-sectional survey in 2006;and data regarding the institutions and physicians providing pelvic floor rehabilitation services from 2005 to 2019.The weighted prevalence of female UI and its subtypes,including stress UI(SUI),urgency UI(UUI),and mixed UI(MUI),were estimated as primary outcomes.Knowledge,attitude and care-seeking behaviors of UI were evaluated.It was found that the weighted prevalence of female UI was 16.0%(95%CI,13.3%–19.1%)with SUI remaining the predominant subtype(7.0%)in 2021,followed by MUI(6.5%)and UUI(1.9%).The estimated absolute number of Chinese adult women with UI was 85.8 million in 2021.52.7%(95%CI,45.9%–59.4%)of women were aware that UI was a medical condition,and only 10.1%of women with UI sought health care.After 15 years of development,there were 8400 pelvic floor rehabilitation institutions and nearly 10,000 relevant physicians in ChinaDthey were found to be associated with UI prevalence.The UI prevalence in China was significantly lower in 2021 compared to that in 2006.Despite the achievement,UI remains a public health problem,especially given China's fast aging and three-child policy.More innovations,especially those that can facilitate care seeking,are needed to address this prevalent yet treatable condition.展开更多
基金This work was supported by the National Natural Science Foundation of China (No.81472886 and No. 81172588).
文摘Keloid may induce severe impairment of life quality for the patients,although keloid is a cutaneous benign tumor.Collagen triple helix repeat containing protein 1 (Cthrc1) was identified as a novel gene that was originally found in adventitial fibroblasts after arterial injury.To address the role of Cthrcl in keloid,the expression level of Cthrcl was assessed in normal skin and keloid tissue,as well as in normal fibroblasts (NFs)and keloid fibroblasts (KFs)by using quantitative PCR,Western blotting and immunohistochemical analysis.The results showed that Cthrcl was increased in keloid tissue and KFs as compared with normal skin and NFs.Meanwhile,CCK8 and Transwell assays found the cellular proliferation and migration of KFs were increased as compared with NFs.Further,to verify the function of Cthrcl in NFs and K.Fs,we increased Cthrcl expression by transfecting lentivirns (LV) vectors LV-Cthrcl.The cellular proliferation and migration,collagen synthesis and the influence on TGF-β and YAP signaling were tested.The cellular proliferation and migration were increased in NFs-Cthrcl as compared with NFs-control.Meanwhile,YAP expression and nuclear-location was increased in NFs-Cthrcl.On the contrary,when Cthrcl was overexpressed in KFs,the cellular migration was suppressed and YAP expression was reduced and transferred to cytoplasm in KFs-Cthrcl as compared with KFs-control.But the expression level of collagen I was decreased and pSmad2/3 nucleus transfer was suppressed in both NFs-Cthrc1 and KFs-Cthrc1 by using Western blotting and immunofluorescence.Increased Cthrcl activated NFs by promoting YAP nucleus translocation,whereas suppressed KFs by inhibiting YAP nucleus translocation.Enhanced Cthrcl decreased collagen I in both NFs and KFs by inhibiting TGF-β/Smad pathway.In conclusion,Cthrcl may play a role in the pathogenesis of keloid by inhibiting collagen synthesis and fibroblasts migration via suppressing TGF-β/Smad pathway and YAP nucleus translocation.
基金supported by the National Key Research and Development Program of China(2021YFC2701300,2023YFC2706000 and 2018YFC2002201)the National Natural Science Foundation of China(72104247)the National High Level Hospital Clinical Research Funding(2022-PUMCH-B-087,2022-PUMCH-A-023)。
文摘Urinary incontinence(UI)is a disease that quietly yet seriously impacts women's health and represents a global health burden that is often neglected.This study aims to systematically assess the prevalence and dynamics of female UI in China,and can inform further policies and have international implications.This study used three nationwide investigations:A national cross-sectional survey in 2021;another nationwide cross-sectional survey in 2006;and data regarding the institutions and physicians providing pelvic floor rehabilitation services from 2005 to 2019.The weighted prevalence of female UI and its subtypes,including stress UI(SUI),urgency UI(UUI),and mixed UI(MUI),were estimated as primary outcomes.Knowledge,attitude and care-seeking behaviors of UI were evaluated.It was found that the weighted prevalence of female UI was 16.0%(95%CI,13.3%–19.1%)with SUI remaining the predominant subtype(7.0%)in 2021,followed by MUI(6.5%)and UUI(1.9%).The estimated absolute number of Chinese adult women with UI was 85.8 million in 2021.52.7%(95%CI,45.9%–59.4%)of women were aware that UI was a medical condition,and only 10.1%of women with UI sought health care.After 15 years of development,there were 8400 pelvic floor rehabilitation institutions and nearly 10,000 relevant physicians in ChinaDthey were found to be associated with UI prevalence.The UI prevalence in China was significantly lower in 2021 compared to that in 2006.Despite the achievement,UI remains a public health problem,especially given China's fast aging and three-child policy.More innovations,especially those that can facilitate care seeking,are needed to address this prevalent yet treatable condition.
