Clinically differentiating bipolarⅡdisorder(BD-Ⅱ)from major depressive disorder(MDD)remains a significant challenge in modern psychiatry.These two conditions share substantial clinical symptomatology,making accurate...Clinically differentiating bipolarⅡdisorder(BD-Ⅱ)from major depressive disorder(MDD)remains a significant challenge in modern psychiatry.These two conditions share substantial clinical symptomatology,making accurate diagnosis difficult in routine clinical practice.Misdiagnosis may lead to inappropriate treatment strategies,increased psychological and physical burdens,reduced quality of life,and impaired social functioning.Genetic overlap may partially explain the clinical similarities between MDD and BD-Ⅱ,and biomarkers along with neuroimaging techniques are receiving increasing attention as tools to aid in diagnosis.For example,electroencephalography has been shown to effectively distinguish between unipolar depression and bipolar depression;serum levels of glycogen synthase kinase-3 have also been investigated as a potential tool for differentiating between the two disorders.A comprehensive assessment integrating clinical characteristics,genetic basis research,and multimodal evaluations using neuroimaging and biomarkers through a multidisciplinary approach will help enhance clinicians'ability to distinguish between MDD and BD-Ⅱ.By improving diagnostic accuracy,more personalized and effective treatment strategies can be developed,ultimately improving patients'health outcomes and quality of life.展开更多
Objective This study examines the sequential mediating roles of body pain and self-reported health in the association between sleep duration and self-reported life satisfaction among elderly Chinese adults.Methods Dat...Objective This study examines the sequential mediating roles of body pain and self-reported health in the association between sleep duration and self-reported life satisfaction among elderly Chinese adults.Methods Data from the fifth wave of the China Health and Retirement Longitudinal Survey(CHARLS)were used to analyse the relationships between sleep duration and body pain,self-reported health,and life satisfaction through logistic regression and Restricted Cubic Spline(RCS)analyses.The sequential mediation effects of body pain and self-reported health status were examined via chain mediation analysis.Results Logistic regression analysis showed that sleeping fewer than 6 hours or 6-7 hours was linked to higher risks of body pain,poor health,and dissatisfaction with life compared to sleeping 7-8 hours(all P<0.05).Additionally,those sleeping more than 9 hours also had increased risks of poor health and dissatisfaction with life compared to those sleeping 7-8 hours(all P<0.05).Chain mediation analysis showed that body pain and self-reported health status sequentially mediated 46.15%of the association between sleep duration and life satisfaction.Conclusion Body pain and self-reported health may shape the relationship between sleep duration and life satisfaction in elderly Chinese adults.展开更多
Overproduction of reactive oxygen species(ROS) following ischemic injury triggers an inflammatory response,significantly impeding neurological functional recovery.Nanozymes with potent antioxidative and anti-inflammat...Overproduction of reactive oxygen species(ROS) following ischemic injury triggers an inflammatory response,significantly impeding neurological functional recovery.Nanozymes with potent antioxidative and anti-inflammatory effects thus offer great potential for ischemic stroke treatment.In this study,we developed an ischemia-homing nanozyme by combining melatonin(MT)-loaded honeycomb manganese dioxide(MnO_(2)) nanoflowers with M2-type microglia membranes to rescue the ischemic penumbra.The surface-engineered M2-type microglia membranes provided intrinsic ischemia-homing and blood-brain barrier(BBB)-crossing properties to the biomimetic nanozymes.This nanozyme can not only transforms harmfulsuperoxide anion radicals(^(·)O^(2-)) and hydrogen peroxide(H_(2)O_(2)) into harmless water and oxygen but also scavenges highly toxic hydroxyl radicals(^(·)OH),dramatically lowering intracellular ROS levels.More importantly,the biomimetic nanoparticles reduce cerebral infarct areas and provide significant neuroprotection against ischemic stroke by lowering oxidative stress,inhibiting cell apoptosis,and decreasing inflammation.This study may offer a viable approach for the use of nanozymes in treating ischemic stroke.展开更多
The performance of data restore is one of the key indicators of user experience for backup storage systems.Compared to the traditional offline restore process,online restore reduces downtime during backup restoration,...The performance of data restore is one of the key indicators of user experience for backup storage systems.Compared to the traditional offline restore process,online restore reduces downtime during backup restoration,allowing users to operate on already restored files while other files are still being restored.This approach improves availability during restoration tasks but suffers from a critical limitation:inconsistencies between the access sequence and the restore sequence.In many cases,the file a user needs to access at a given moment may not yet be restored,resulting in significant delays and poor user experience.To this end,we present Histore,which builds on the user’s historical access sequence to schedule the restore sequence,in order to reduce users’access delayed time.Histore includes three restore approaches:(i)the frequency-based approach,which restores files based on historical file access frequencies and prioritizes ensuring the availability of frequently accessed files;(ii)the graph-based approach,which preferentially restores the frequently accessed files as well as their correlated files based on historical access patterns,and(iii)the trie-based approach,which restores particular files based on both users’real-time and historical access patterns to deduce and restore the files to be accessed in the near future.We implement a prototype of Histore and evaluate its performance from multiple perspectives.Trace-driven experiments on two datasets show that Histore significantly reduces users’delay time by 4-700×with only 1.0%-14.5%additional performance overhead.展开更多
BACKGROUND Gastric precancerous lesions(GPL)precede the development of gastric cancer(GC).They are characterized by gastric mucosal intestinal metaplasia and dysplasia caused by various factors such as inflammation,ba...BACKGROUND Gastric precancerous lesions(GPL)precede the development of gastric cancer(GC).They are characterized by gastric mucosal intestinal metaplasia and dysplasia caused by various factors such as inflammation,bacterial infection,and injury.Abnormalities in autophagy and glycolysis affect GPL progression,and their effective regulation can aid in GPL treatment and GC prevention.Xiaojianzhong decoction(XJZ)is a classic compound for the treatment of digestive system diseases in ancient China which can inhibit the progression of GPL.However,its specific mechanism of action is still unclear.AIM To investigate the therapeutic effects of XJZ decoction on a rat GPL model and the mechanisms underlying its effects on autophagy and glycolysis regulation in GPLs.METHODS Wistar rats were randomly divided into six groups of five rats each and all groups except the control group were subjected to GPL model construction for 18 wk.The rats’body weight was monitored every 2 wk starting from the beginning of modeling.Gastric histopathology was examined using hematoxylin-eosin staining and Alcian blue-periodic acid-Schiff staining.Autophagy was observed using transmission electron microscopy.The expressions of autophagy,hypoxia,and glycolysis related proteins in gastric mucosa were detected using immunohistochemistry and immunofluorescence.The expressions of the following proteins in gastric tissues:B cell lymphoma/Leukemia-2 and adenovirus E1B19000 interacting protein 3(Bnip-3),microtubule associated protein 1 light chain 3(LC-3),moesin-like BCL2-interacting protein 1(Beclin-1),phosphatidylinositol 3-kimase(PI3K),protein kinase B(AKT),mammalian target of rapamycin(mTOR),p53,AMP-activated protein kinase(AMPK),and Unc-51 like kinase 1(ULK1)were detected using western blot.The relative expressions of autophagy,hypoxia,and glycolysis related mRNA in gastric tissues was detected using reverse transcription-polymerase chain reaction.RESULTS Treatment with XJZ increased the rats’body weight and improved GPL-related histopathological manifestations.It also decreased autophagosome and autolysosome formation in gastric tissues and reduced Bnip-3,Beclin-1,and LC-3II expressions,resulting in inhibition of autophagy.Moreover,XJZ down-regulated glycolysis-related monocarboxylate transporter(MCT1),MCT4,and CD147 expressions.XJZ prevented the increase of autophagy level by decreasing gastric mucosal hypoxia,activating the PI3K/AKT/mTOR pathway,inhibiting the p53/AMPK pathway activation and ULK1 Ser-317 and Ser-555 phosphorylation.In addition,XJZ improved abnormal gastric mucosal glucose metabolism by ameliorating gastric mucosal hypoxia and inhibiting ULK1 expression.CONCLUSION This study demonstrates that XJZ may inhibit autophagy and glycolysis in GPL gastric mucosal cells by improving gastric mucosal hypoxia and regulating PI3K/AKT/mTOR and p53/AMPK/ULK1 signaling pathways,providing a feasible strategy for the GPL treatment.展开更多
Objective:To evaluate the effects of tetrandrine citrate, a novel tetrandrine salt with high water solubility, on the growth of imatinib (IM)-resistant chronic myeloid leukemia (CML) in vitro and in vivo, and reveal a...Objective:To evaluate the effects of tetrandrine citrate, a novel tetrandrine salt with high water solubility, on the growth of imatinib (IM)-resistant chronic myeloid leukemia (CML) in vitro and in vivo, and reveal action molecular mechanisms. Methods:Cell viability in vitro was measured using methyl thiazolyl tetrazolium (MTT) assay. CML cell growth in vivo was assessed using a xenograft model in nude mice. Bcr-Abl and β-catenin protein levels were determined using Western blotting. Bcr-Abl messenger RNA (mRNA) was measured by reverse transcription polymerase chain reaction (RT-PCR). Flow cytometry (FCM) was used to determine cell cycle status. Results:Tetrandrine citrate inhibited the growth of IM-resistant K562 cells, primary leukemia cells, and primitive CD34 + leukemia cells, and their inhibition concentration that inhibited 50% of target cells (IC 50 ) ranged from 1.20 to 2.97 μg/ml. In contrast, tetrandrine citrate did not affect normal blood cells under the same conditions, and IC 50 values were about 10.12-13.11 μg/ml. Oral administration of tetrandrine citrate caused complete regression of IM-resistant K562 xeno-grafts in nude mice without overt toxicity. Western blot results revealed that treatment of IM-resistant K562 cells with tetrandrine citrate resulted in a significant decrease of both p210 Bcr-Abl and β-catenin proteins, but IM did not affect the Bcr-Abl protein levels. Proteasome inhibitor, MG132, did not prevent tetrandrine-mediated decrease of the p210 Bcr-Abl protein. RT-PCR results showed that tetrandrine treatment caused a decrease of Bcr-Abl mRNA. FCM analysis indicated that tetrandrine induced gap 1 (G 1 ) arrest in CML cells. Conclusions:Tetrandrine citrate is a novel orally active tetrandrine salt with potent anti-tumor activity against IM-resistant K562 cells and CML cells. Tetrandrine citrate-induced growth inhibition of leukemia cells may be involved in the depletion of p210 Bcr-Abl mRNA and β-catenin protein.展开更多
Hypoxia is a feature of solid tumors and it hinders the therapeutic efficacy of oxygen-dependent cancer treatment.Herein,we have developed all-organic oxygen-independent hybrid nanobullets ZPA@HA-ACVA-AZ for the“prec...Hypoxia is a feature of solid tumors and it hinders the therapeutic efficacy of oxygen-dependent cancer treatment.Herein,we have developed all-organic oxygen-independent hybrid nanobullets ZPA@HA-ACVA-AZ for the“precise strike”of hypoxic tumors through the dual-targeting effects from surface-modified hyaluronic acid(HA)and hypoxia-dependent factor carbonic anhydrase IX(CA IX)-inhibitor acetazolamide(AZ).The core of nanobullets is the special zinc(II)phthalocyanine aggregates(ZPA)which could heat the tumor tissues upon 808-nm laser irradiation for photothermal therapy(PTT),along with the alkyl chain-functionalized thermally decomposable radical initiator ACVA-HDA on the side chain of HA for providing oxygen-independent alkyl radicals for ablating hypoxic cancer cells by thermodynamic therapy(TDT).The results provide important evidence that the combination of reverse hypoxia hallmarks CA IX as targets for inhibition by AZ and synergistic PTT/TDT possess incomparable therapeutic advantages over traditional(reactive oxygen species(ROS)-mediated)cancer treatment for suppressing the growth of both hypoxic tumors and their metastasis.展开更多
Agronomic measures are the key to promote the sustainable development of ratoon rice by reducing the damage from mechanical crushing to the residual stubble of the main crop, thereby mitigating the impact on axillary ...Agronomic measures are the key to promote the sustainable development of ratoon rice by reducing the damage from mechanical crushing to the residual stubble of the main crop, thereby mitigating the impact on axillary bud sprouting and yield formation in ratoon rice. This study used widely recommended conventional rice Jiafuzhan and hybrid rice Yongyou 2640 as the test materials to conduct a four-factor block design field experiment in a greenhouse of the experimental farm of Fujian Agricultural and Forestry University, China from 2018 to 2019.The treatments included fertilization and no fertilization, alternate wetting and drying irrigation and continuous water flooding irrigation, and plots with and without artificial crushing damage on the rice stubble. At the same time, a 13C stable isotope in-situ detection technology was used to fertilize the pot experiment. The results showed significant interactions among varieties, water management, nitrogen application and stubble status.Relative to the long-term water flooding treatment, the treatment with sequential application of nitrogen fertilizer coupled with moderate field drought for root-vigor and tiller promotion before and after harvesting of the main crop, significantly improved the effective tillers from low position nodes. This in turn increased the effective panicles per plant and grains per panicle by reducing the influence of artificial crushing damage on rice stubble and achieving a high yield of the regenerated rice. Furthermore, the partitioning of 13C assimilates to the residual stubble and its axillary buds were significantly improved at the mature stage of the main crop, while the translocation rate to roots and rhizosphere soil was reduced at the later growth stage of ratooning season rice. This was triggered by the metabolism of hormones and polyamines at the stem base regulated by the interaction of water and fertilizer at this time. We therefore suggest that to achieve a high yield of ratoon rice with low stubble height under mechanized harvesting, the timely application of nitrogen fertilizer is fundamental,coupled with moderate field drying for root-vigor preservation and tiller promotion before and after the mechanical harvesting of the main crop.展开更多
Hierarchical porous carbons (HPCs) are obtained via in-situ activation of interpenetrating polymer networks (IPNs) obtained from simultaneous polymerization of resorcinol/formaldehyde (R/F) and polyacrylamide (PAM). T...Hierarchical porous carbons (HPCs) are obtained via in-situ activation of interpenetrating polymer networks (IPNs) obtained from simultaneous polymerization of resorcinol/formaldehyde (R/F) and polyacrylamide (PAM). The hierarchically micro-, meso-and macroporous structure of as-prepared HPCs is attributed to the synergistic pore-forming effect of PAM and KOH, including PAM decomposition, KOH chemical activation, and a foaming process of potassium polyacrylate formed by partial hydrolysis of PAM in KOH aqueous solution. The typical HPC electrode with the highest surface area (2544 m2/g) shows a high specific capacitance of 261 F/g at 1.0 A/g and a superior rate capability of 216 F/g at 20 A/g in alkaline electrolyte. Moreover, the electrode maintains the capacitance retention of 90.8% after 10000 chargingdischarging cycles at 1.0 A/g, exhibiting long cycling life. This study highlights a new avenue towards IPNs-derived carbons with unique pore structure for promising electrochemical applications.展开更多
The pathogenesis of very-long-chain acyl-CoA dehydrogenase(VLCAD)deficiency is highly heterogeneous and still unclear.Additional novel variants have been recently detected in the population.The molecular and cellular ...The pathogenesis of very-long-chain acyl-CoA dehydrogenase(VLCAD)deficiency is highly heterogeneous and still unclear.Additional novel variants have been recently detected in the population.The molecular and cellular effects of these previously unreported variants are still poorly understood and require further characterization.To address this problem,we have evaluated the various functions and biochemical consequences of six novel missense variants that lead to mild VLCAD deficiency.Marked deficiencies in fatty acid oxidation(FAO)and other mitochondrial defects were observed in cells carrying one of these six variants(c.541 C>T,c.863 T>G,c.895 A>G,c.1238 T>C,c.1276 G>A,and c.1505 T>A),including reductions in mitochondrial respiratory-chain function and adenosine teriphosphate(ATP)production,and increased levels of mitochondrial reactive oxygen species(ROS).Intriguingly,higher apoptosis levels were found in cells carrying the mutant VLCAD under glucose-limited stress.Moreover,the stability of the mutant homodimer was disturbed,and major conformational changes in each mutant VLCAD structure were predicted by molecular dynamics(MD)simulation.The data presented here may provide valuable information for improving management of diagnosis and treatment of VLCAD deficiency and for a better understanding of the general molecular bases of disease variability.展开更多
With the introduction of software defined hardware by DARPA Electronics Resurgence Initiative,software definition will be the basic attribute of information system.Benefiting from boundary certainty and algorithm aggr...With the introduction of software defined hardware by DARPA Electronics Resurgence Initiative,software definition will be the basic attribute of information system.Benefiting from boundary certainty and algorithm aggregation of domain applications,domain-oriented computing architecture has become the technical direction that considers the high flexibility and efficiency of information system.Aiming at the characteristics of data-intensive computing in different scenarios such as Internet of Things(IoT),big data,artificial intelligence(AI),this paper presents a domain-oriented software defined computing architecture,discusses the hierarchical interconnection structure,hybrid granularity computing element and its computational kernel extraction method,finally proves the flexibility and high efficiency of this architecture by experimental comparison.展开更多
Mature pollen germinates rapidly on the stigma,extending its pollen tube to deliver sperm cells to the ovule for fertilization.The success of this process is an important factor that limits output.The flavonoid conten...Mature pollen germinates rapidly on the stigma,extending its pollen tube to deliver sperm cells to the ovule for fertilization.The success of this process is an important factor that limits output.The flavonoid content increased signi ficantly during pollen germination and pollen tube growth,which suggests it may play an important role in these processes.However,the speci fi c mechanism of this involvement has been little researched.Our previous research found that hyperoside can prolong the flowering period of Abelmoschus esculentus(okra),but its speci fic mechanism is still unclear.Therefore,in this study,we focused on the effect of hyperoside in regulating the actin-depolymerizing factor(ADF),which further affects the germination and growth of pollen.We found that hyperoside can prolong the effective pollination period of okra by 2-3-fold and promote the growth of pollen tubes in the style.Then,we used Nicotiana benthamiana cells as a research system and found that hyperoside accelerates the depolymerization of intercellular micro fi laments.Hyperoside can promote pollen germination and pollen tube elongation in vitro.Moreover,AeADFl was identi fied out of all AeADF genes as being highly expressed in pollen tubes in response to hyperoside.In addition,hyperoside promoted AeADF1-mediated micro filament dissipation according to micro filament severing experiments in vitro.In the pollen tube,the gene expression of AeADFl was reduced to 1/5 by oligonucleotide transfection.The decrease in the expression level of AeADFl partially reduced the promoting effect of hyperoside on pollen germination and pollen tube growth.This research provides new research directions for flavonoids in reproductive development.展开更多
This paper investigates an unmanned aerial vehicle(UAV)-assisted multi-object offloading scheme for blockchain-enabled Vehicle-to-Everything(V2X)systems.Due to the presence of an eavesdropper(Eve),the system’s com-mu...This paper investigates an unmanned aerial vehicle(UAV)-assisted multi-object offloading scheme for blockchain-enabled Vehicle-to-Everything(V2X)systems.Due to the presence of an eavesdropper(Eve),the system’s com-munication links may be insecure.This paper proposes deploying an intelligent reflecting surface(IRS)on the UAV to enhance the communication performance of mobile vehicles,improve system flexibility,and alleviate eavesdropping on communication links.The links for uploading task data from vehicles to a base station(BS)are protected by IRS-assisted physical layer security(PLS).Upon receiving task data,the computing resources provided by the edge computing servers(MEC)are allocated to vehicles for task execution.Existing blockchain-based computation offloading schemes typically focus on improving network performance,such as minimizing energy consumption or latency,while neglecting the Gas fees for computation offloading and the costs required for MEC computation,leading to an imbalance between service fees and resource allocation.This paper uses a utility-oriented computation offloading scheme to balance costs and resources.This paper proposes alternating phase optimization and power optimization to optimize the energy consumption,latency,and communication secrecy rate,thereby maximizing the weighted total utility of the system.Simulation results demonstrate a notable enhancement in the weighted total system utility and resource utilization,thereby corroborating the viability of our approach for practical applications.展开更多
Recognized as a pivotal facet in Beyond Fifth-Generation(B5G)and the upcoming Sixth-Generation(6G)wireless networks,Unmanned Aerial Vehicle(UAV)communications pose challenges due to limited capabilities when serving a...Recognized as a pivotal facet in Beyond Fifth-Generation(B5G)and the upcoming Sixth-Generation(6G)wireless networks,Unmanned Aerial Vehicle(UAV)communications pose challenges due to limited capabilities when serving as mobile base stations,leading to suboptimal service for edge users.To address this,the collaborative formation of Coordinated Multi-Point(CoMP)networks proves instrumental in alleviating the issue of the poor Quality of Service(QoS)at edge users in the network periphery.This paper introduces a groundbreaking solution,the Hybrid Uplink-Downlink Non-Orthogonal Multiple Access(HUD-NOMA)scheme for UAV-aided CoMP networks.Leveraging network coding and NOMA technology,our proposed HUD-NOMA effectively enhances transmission rates for edge users,notwithstanding a minor reduction in signal reception reliability for strong signals.Importantly,the system’s overall sum rate is elevated.The proposed HUD-NOMA demonstrates resilience against eavesdroppers by effectively managing intended interferences without the need for additional artificial noise injection.The study employs a stochastic geometry approach to derive the Secrecy Outage Probability(SOP)for the transmissions in the CoMP network,revealing superior performance in transmission rates and lower SOP compared to existing methods through numerical verification.Furthermore,guided by the theoretical SOP derivation,this paper proposes a power allocation strategy to further reduce the system’s SOP.展开更多
Background Intramuscular fat is an important factor in evaluating pork quality and varies widely among different pig breeds.However,the regulatory mechanism of circular RNAs(circRNAs)in lipid metabolism remains largel...Background Intramuscular fat is an important factor in evaluating pork quality and varies widely among different pig breeds.However,the regulatory mechanism of circular RNAs(circRNAs)in lipid metabolism remains largely unexplored.Results We combined circRNA-seq and Ribo-seq data to screen a total of 18 circRNA candidates with coding potential,and circANKRD17 was found to be significantly elevated in the longissimus dorsi muscle of Lantang piglets,with a length of 1,844 nucleotides.Using single-cell sequencing,we identified 477 differentially expressed genes in IMF cells between Lantang and Landrace piglets,with enrichment in the PPAR signaling pathway.These genes included FABP4,FABP5,CPT1A,and UBC,consistent with the high levels of acylcarnitines observed in the longissimus dorsi muscles of the Lantang breed,as determined by lipidomic analysis.Further in vitro and in vivo experiments indicated that circANKRD17 can regulate lipid metabolism through various mechanisms involving the PPAR pathway,including promoting adipocyte differentiation,fatty acid transport and metabolism,triglyceride synthesis,and lipid droplet formation and maturation.In addition,we discovered that circANKRD17 has an open reading frame and can be translated into a novel 571-amino-acid protein that promotes lipid metabolism.Conclusions Our research provides new insights into the role of protein-coding circANKRD17,especially concerning the metabolic characteristics of pig breeds with higher intramuscular fat content.展开更多
BACKGROUND Owing to the absence of specific symptoms in early-stage gastric cancer,most patients are diagnosed at intermediate or advanced stages.As a result,treatment often shifts from surgery to other therapies,with...BACKGROUND Owing to the absence of specific symptoms in early-stage gastric cancer,most patients are diagnosed at intermediate or advanced stages.As a result,treatment often shifts from surgery to other therapies,with chemotherapy and targeted therapies being the primary options for advanced gastric cancer treatment.A total of 116 patients with advanced gastric cancer,admitted from January 2021 to December 2023,were selected and divided into two groups of 58 each using the random number table method.The control group received FOLFOX4 chemothe-rapy(oxaliplatin+calcium+folinate+5-fluorouracil)combined with intravenous sindilizumab.The observation group received the same treatment as the control group,supplemented by oral administration of Senqi Shiyiwei granules.Both groups underwent treatment cycles of 3 weeks,with a minimum of two cycles.The therapeutic efficacy,immune mechanisms,and treatment-related toxicity and side effects were compared between the groups.The objective remission rate in the observation group(55.17%)was higher than that of the control group(36.21%)(P<0.05).After two treatment cycle,CD3+,CD4+,and CD4+/CD8+levels were higher in the observation group compared to the control group,while CD8+,regulatory T cells,and natural killer cells were lower(P<0.05).Additionally,the incidence of leukopenia,nausea,and vomiting was lower in observed group(P<0.05).No significant differences were observed in the incidence of other adverse reactions(P>0.05).CONCLUSION Adjuvant therapy with Shenqixian granules may enhance the efficacy of simudizumab combined with FOLFOX4 chemotherapy in advanced gastric cancer and the immune function by increasing immune cell counts,making it a valuable option in clinical treatment.展开更多
目的探讨单侧上尿路梗阻解除前后肾皮质厚度(RCT)与肾小球滤过率(GFR)的关系。方法选取2015年7月-2016年6月该院泌尿外科收治的63例上泌尿道梗阻患者为研究对象。其中,男34例,女29例,年龄22~73(52.21±11.56)岁;手术方式:输尿管镜碎...目的探讨单侧上尿路梗阻解除前后肾皮质厚度(RCT)与肾小球滤过率(GFR)的关系。方法选取2015年7月-2016年6月该院泌尿外科收治的63例上泌尿道梗阻患者为研究对象。其中,男34例,女29例,年龄22~73(52.21±11.56)岁;手术方式:输尿管镜碎石+取石术24例,一期经皮肾造瘘+二期经皮肾镜取石术(PCNL)21例,一期PCNL 18例。采用腹部增强CT、单光子发射计算机断层(SPECT)扫描分别测量RCT和GFR,根据术前患肾GFR情况分为3组:A组(中度肾损害,15.0 ml/min≤GFR<30.0 ml/min)、B组(重度肾损害,7.5 ml/min≤GFR<15.0 ml/min)、C组(极重度肾损害,GFR<7.5 ml/min),比较3组的手术前后的GFR变化。采用简单线性相关分析A和B组患者术前RCT与各指标的相关性。结果 9例患者因肾积水严重未测量RCT,患者的术前RCT>10 mm 20例,5~10 mm 23例,<5 mm 11例,平均值为(10.75±4.91)mm。A组术前RCT明显高于B组,差异有统计学意义(P<0.05)。3组患者术后1和9个月的GFR均明显高于同组术前,差异均有统计学意义(P<0.05)。A组术后1和9个月的GFR均明显高于另外两组,B组术后9个月的GFR明显高于C组,差异均有统计学意义(P<0.05),但术后1个月B组和C组的GFR差异无统计学意义(P>0.05)。B组术后1个月的GFR恢复值明显低于另外两组,差异有统计学意义(P<0.05),3组术后9个月的GFR恢复值比较差异无统计学意义(P>0.05)。术前RCT与术前GFR(r=0.613)、术后1个月GFR(r=0.697)及恢复值(r=0.552)、术后9个月GFR(r=0.589)及恢复值(r=0.488)均呈显著正相关,差异有统计学意义(P<0.05)。结论解除上泌尿道梗阻后患者的肾功能得到不同程度的恢复,即使是极重度肾功能损伤者,多数均能挽回患肾。术前RCT能有助于判断围手术期的肾功能,尤其是术后肾功能恢复情况。展开更多
文摘Clinically differentiating bipolarⅡdisorder(BD-Ⅱ)from major depressive disorder(MDD)remains a significant challenge in modern psychiatry.These two conditions share substantial clinical symptomatology,making accurate diagnosis difficult in routine clinical practice.Misdiagnosis may lead to inappropriate treatment strategies,increased psychological and physical burdens,reduced quality of life,and impaired social functioning.Genetic overlap may partially explain the clinical similarities between MDD and BD-Ⅱ,and biomarkers along with neuroimaging techniques are receiving increasing attention as tools to aid in diagnosis.For example,electroencephalography has been shown to effectively distinguish between unipolar depression and bipolar depression;serum levels of glycogen synthase kinase-3 have also been investigated as a potential tool for differentiating between the two disorders.A comprehensive assessment integrating clinical characteristics,genetic basis research,and multimodal evaluations using neuroimaging and biomarkers through a multidisciplinary approach will help enhance clinicians'ability to distinguish between MDD and BD-Ⅱ.By improving diagnostic accuracy,more personalized and effective treatment strategies can be developed,ultimately improving patients'health outcomes and quality of life.
基金supported by This work was supported by the Ministry of Science and Technology of the People’s Republic of China(STI2030-Major Projects2021ZD0201900)。
文摘Objective This study examines the sequential mediating roles of body pain and self-reported health in the association between sleep duration and self-reported life satisfaction among elderly Chinese adults.Methods Data from the fifth wave of the China Health and Retirement Longitudinal Survey(CHARLS)were used to analyse the relationships between sleep duration and body pain,self-reported health,and life satisfaction through logistic regression and Restricted Cubic Spline(RCS)analyses.The sequential mediation effects of body pain and self-reported health status were examined via chain mediation analysis.Results Logistic regression analysis showed that sleeping fewer than 6 hours or 6-7 hours was linked to higher risks of body pain,poor health,and dissatisfaction with life compared to sleeping 7-8 hours(all P<0.05).Additionally,those sleeping more than 9 hours also had increased risks of poor health and dissatisfaction with life compared to those sleeping 7-8 hours(all P<0.05).Chain mediation analysis showed that body pain and self-reported health status sequentially mediated 46.15%of the association between sleep duration and life satisfaction.Conclusion Body pain and self-reported health may shape the relationship between sleep duration and life satisfaction in elderly Chinese adults.
基金supported by National Key R&D Program of China (No.2022YFC3501700)National Natural Science Foundation of China (No.82274059)+3 种基金Naval Military Medical University,Far East Talent Project (No.SL-33)Talent Project established by Chinese Pharmaceutical Association Hospital Pharmacy department (No.CPA-Z05-ZC-2024-003)Shanghai Oriental Talent Plan Youth Program (formerly Shanghai Young Top-Notch Talent) (2023)the Baoshan District Medical Key Science (Specialty) and Specialty Brand Construction Project (No.BSZK-2023-A12)。
文摘Overproduction of reactive oxygen species(ROS) following ischemic injury triggers an inflammatory response,significantly impeding neurological functional recovery.Nanozymes with potent antioxidative and anti-inflammatory effects thus offer great potential for ischemic stroke treatment.In this study,we developed an ischemia-homing nanozyme by combining melatonin(MT)-loaded honeycomb manganese dioxide(MnO_(2)) nanoflowers with M2-type microglia membranes to rescue the ischemic penumbra.The surface-engineered M2-type microglia membranes provided intrinsic ischemia-homing and blood-brain barrier(BBB)-crossing properties to the biomimetic nanozymes.This nanozyme can not only transforms harmfulsuperoxide anion radicals(^(·)O^(2-)) and hydrogen peroxide(H_(2)O_(2)) into harmless water and oxygen but also scavenges highly toxic hydroxyl radicals(^(·)OH),dramatically lowering intracellular ROS levels.More importantly,the biomimetic nanoparticles reduce cerebral infarct areas and provide significant neuroprotection against ischemic stroke by lowering oxidative stress,inhibiting cell apoptosis,and decreasing inflammation.This study may offer a viable approach for the use of nanozymes in treating ischemic stroke.
基金supported in part by National Key R&D Program of China(2022YFB4501200),National Natural Science Foundation of China(62332018)Science and Technology Program(2024NSFTD0031,2024YFHZ0339 and 2025ZNSFSC0497).
文摘The performance of data restore is one of the key indicators of user experience for backup storage systems.Compared to the traditional offline restore process,online restore reduces downtime during backup restoration,allowing users to operate on already restored files while other files are still being restored.This approach improves availability during restoration tasks but suffers from a critical limitation:inconsistencies between the access sequence and the restore sequence.In many cases,the file a user needs to access at a given moment may not yet be restored,resulting in significant delays and poor user experience.To this end,we present Histore,which builds on the user’s historical access sequence to schedule the restore sequence,in order to reduce users’access delayed time.Histore includes three restore approaches:(i)the frequency-based approach,which restores files based on historical file access frequencies and prioritizes ensuring the availability of frequently accessed files;(ii)the graph-based approach,which preferentially restores the frequently accessed files as well as their correlated files based on historical access patterns,and(iii)the trie-based approach,which restores particular files based on both users’real-time and historical access patterns to deduce and restore the files to be accessed in the near future.We implement a prototype of Histore and evaluate its performance from multiple perspectives.Trace-driven experiments on two datasets show that Histore significantly reduces users’delay time by 4-700×with only 1.0%-14.5%additional performance overhead.
基金Supported by the Shaanxi Science and Technology overall Planning and Innovation Project,No.2016KTTSSF01-05Key R&D projects in Shaanxi Province,No.2022ZDLSF05-10Shaanxi University of Chinese Medicine Discipline Innovation Team Construction Project,No.2019-YL-05.
文摘BACKGROUND Gastric precancerous lesions(GPL)precede the development of gastric cancer(GC).They are characterized by gastric mucosal intestinal metaplasia and dysplasia caused by various factors such as inflammation,bacterial infection,and injury.Abnormalities in autophagy and glycolysis affect GPL progression,and their effective regulation can aid in GPL treatment and GC prevention.Xiaojianzhong decoction(XJZ)is a classic compound for the treatment of digestive system diseases in ancient China which can inhibit the progression of GPL.However,its specific mechanism of action is still unclear.AIM To investigate the therapeutic effects of XJZ decoction on a rat GPL model and the mechanisms underlying its effects on autophagy and glycolysis regulation in GPLs.METHODS Wistar rats were randomly divided into six groups of five rats each and all groups except the control group were subjected to GPL model construction for 18 wk.The rats’body weight was monitored every 2 wk starting from the beginning of modeling.Gastric histopathology was examined using hematoxylin-eosin staining and Alcian blue-periodic acid-Schiff staining.Autophagy was observed using transmission electron microscopy.The expressions of autophagy,hypoxia,and glycolysis related proteins in gastric mucosa were detected using immunohistochemistry and immunofluorescence.The expressions of the following proteins in gastric tissues:B cell lymphoma/Leukemia-2 and adenovirus E1B19000 interacting protein 3(Bnip-3),microtubule associated protein 1 light chain 3(LC-3),moesin-like BCL2-interacting protein 1(Beclin-1),phosphatidylinositol 3-kimase(PI3K),protein kinase B(AKT),mammalian target of rapamycin(mTOR),p53,AMP-activated protein kinase(AMPK),and Unc-51 like kinase 1(ULK1)were detected using western blot.The relative expressions of autophagy,hypoxia,and glycolysis related mRNA in gastric tissues was detected using reverse transcription-polymerase chain reaction.RESULTS Treatment with XJZ increased the rats’body weight and improved GPL-related histopathological manifestations.It also decreased autophagosome and autolysosome formation in gastric tissues and reduced Bnip-3,Beclin-1,and LC-3II expressions,resulting in inhibition of autophagy.Moreover,XJZ down-regulated glycolysis-related monocarboxylate transporter(MCT1),MCT4,and CD147 expressions.XJZ prevented the increase of autophagy level by decreasing gastric mucosal hypoxia,activating the PI3K/AKT/mTOR pathway,inhibiting the p53/AMPK pathway activation and ULK1 Ser-317 and Ser-555 phosphorylation.In addition,XJZ improved abnormal gastric mucosal glucose metabolism by ameliorating gastric mucosal hypoxia and inhibiting ULK1 expression.CONCLUSION This study demonstrates that XJZ may inhibit autophagy and glycolysis in GPL gastric mucosal cells by improving gastric mucosal hypoxia and regulating PI3K/AKT/mTOR and p53/AMPK/ULK1 signaling pathways,providing a feasible strategy for the GPL treatment.
基金supported by the National Natural Science Foundation of China (Nos. 30672381, 30873095, and 81070420)the Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health Talentsthe Zhejiang Provincial Natural Science Foundation of China (Nos. Y206238, Y2080570, and Y2080210)
文摘Objective:To evaluate the effects of tetrandrine citrate, a novel tetrandrine salt with high water solubility, on the growth of imatinib (IM)-resistant chronic myeloid leukemia (CML) in vitro and in vivo, and reveal action molecular mechanisms. Methods:Cell viability in vitro was measured using methyl thiazolyl tetrazolium (MTT) assay. CML cell growth in vivo was assessed using a xenograft model in nude mice. Bcr-Abl and β-catenin protein levels were determined using Western blotting. Bcr-Abl messenger RNA (mRNA) was measured by reverse transcription polymerase chain reaction (RT-PCR). Flow cytometry (FCM) was used to determine cell cycle status. Results:Tetrandrine citrate inhibited the growth of IM-resistant K562 cells, primary leukemia cells, and primitive CD34 + leukemia cells, and their inhibition concentration that inhibited 50% of target cells (IC 50 ) ranged from 1.20 to 2.97 μg/ml. In contrast, tetrandrine citrate did not affect normal blood cells under the same conditions, and IC 50 values were about 10.12-13.11 μg/ml. Oral administration of tetrandrine citrate caused complete regression of IM-resistant K562 xeno-grafts in nude mice without overt toxicity. Western blot results revealed that treatment of IM-resistant K562 cells with tetrandrine citrate resulted in a significant decrease of both p210 Bcr-Abl and β-catenin proteins, but IM did not affect the Bcr-Abl protein levels. Proteasome inhibitor, MG132, did not prevent tetrandrine-mediated decrease of the p210 Bcr-Abl protein. RT-PCR results showed that tetrandrine treatment caused a decrease of Bcr-Abl mRNA. FCM analysis indicated that tetrandrine induced gap 1 (G 1 ) arrest in CML cells. Conclusions:Tetrandrine citrate is a novel orally active tetrandrine salt with potent anti-tumor activity against IM-resistant K562 cells and CML cells. Tetrandrine citrate-induced growth inhibition of leukemia cells may be involved in the depletion of p210 Bcr-Abl mRNA and β-catenin protein.
基金the National Natural Science Foundation of China(51903203,51703178,81770728)the China Postdoctoral Science Foundation(2019M653661,2019M663742)+3 种基金the Natural Science Foundation of Shaanxi Province(2020JQ-046)Natural Science Foundation of Zhejiang Province(LWY20H180002)the Natural Science Foundation of Guangxi Zhuang Autonomous Region(2017GXNSFBA198028).Y.H.,M.G.,Y.S.Z.,G.H.and X.Z.were not supported by any of these fundsinstead,support by MIT,Harvard,Stanford University and the Brigham Research Institute are acknowledged.
文摘Hypoxia is a feature of solid tumors and it hinders the therapeutic efficacy of oxygen-dependent cancer treatment.Herein,we have developed all-organic oxygen-independent hybrid nanobullets ZPA@HA-ACVA-AZ for the“precise strike”of hypoxic tumors through the dual-targeting effects from surface-modified hyaluronic acid(HA)and hypoxia-dependent factor carbonic anhydrase IX(CA IX)-inhibitor acetazolamide(AZ).The core of nanobullets is the special zinc(II)phthalocyanine aggregates(ZPA)which could heat the tumor tissues upon 808-nm laser irradiation for photothermal therapy(PTT),along with the alkyl chain-functionalized thermally decomposable radical initiator ACVA-HDA on the side chain of HA for providing oxygen-independent alkyl radicals for ablating hypoxic cancer cells by thermodynamic therapy(TDT).The results provide important evidence that the combination of reverse hypoxia hallmarks CA IX as targets for inhibition by AZ and synergistic PTT/TDT possess incomparable therapeutic advantages over traditional(reactive oxygen species(ROS)-mediated)cancer treatment for suppressing the growth of both hypoxic tumors and their metastasis.
基金supported by the National Nature Science Foundation of China,the National Key Research and Development Program of China(302001109,2016YFD0300508,2017YFD0301602,2018YFD0301105)the Fujian and Taiwan Cultivation Resources Development and Green Cultivation Coordination Innovation Center,China(Fujian 2011 Project,2015-75)the Natural Science Foundation of Fujian Province,China(2022J01142)。
文摘Agronomic measures are the key to promote the sustainable development of ratoon rice by reducing the damage from mechanical crushing to the residual stubble of the main crop, thereby mitigating the impact on axillary bud sprouting and yield formation in ratoon rice. This study used widely recommended conventional rice Jiafuzhan and hybrid rice Yongyou 2640 as the test materials to conduct a four-factor block design field experiment in a greenhouse of the experimental farm of Fujian Agricultural and Forestry University, China from 2018 to 2019.The treatments included fertilization and no fertilization, alternate wetting and drying irrigation and continuous water flooding irrigation, and plots with and without artificial crushing damage on the rice stubble. At the same time, a 13C stable isotope in-situ detection technology was used to fertilize the pot experiment. The results showed significant interactions among varieties, water management, nitrogen application and stubble status.Relative to the long-term water flooding treatment, the treatment with sequential application of nitrogen fertilizer coupled with moderate field drought for root-vigor and tiller promotion before and after harvesting of the main crop, significantly improved the effective tillers from low position nodes. This in turn increased the effective panicles per plant and grains per panicle by reducing the influence of artificial crushing damage on rice stubble and achieving a high yield of the regenerated rice. Furthermore, the partitioning of 13C assimilates to the residual stubble and its axillary buds were significantly improved at the mature stage of the main crop, while the translocation rate to roots and rhizosphere soil was reduced at the later growth stage of ratooning season rice. This was triggered by the metabolism of hormones and polyamines at the stem base regulated by the interaction of water and fertilizer at this time. We therefore suggest that to achieve a high yield of ratoon rice with low stubble height under mechanized harvesting, the timely application of nitrogen fertilizer is fundamental,coupled with moderate field drying for root-vigor preservation and tiller promotion before and after the mechanical harvesting of the main crop.
基金financially supported by the National Natural Science Foundation of China(Nos. 51772216, 21703161 and 21875165)the Science and Technology Commission of Shanghai Municipality, China (No. 14DZ2261100)the Fundamental Research Funds for the Central Universities
文摘Hierarchical porous carbons (HPCs) are obtained via in-situ activation of interpenetrating polymer networks (IPNs) obtained from simultaneous polymerization of resorcinol/formaldehyde (R/F) and polyacrylamide (PAM). The hierarchically micro-, meso-and macroporous structure of as-prepared HPCs is attributed to the synergistic pore-forming effect of PAM and KOH, including PAM decomposition, KOH chemical activation, and a foaming process of potassium polyacrylate formed by partial hydrolysis of PAM in KOH aqueous solution. The typical HPC electrode with the highest surface area (2544 m2/g) shows a high specific capacitance of 261 F/g at 1.0 A/g and a superior rate capability of 216 F/g at 20 A/g in alkaline electrolyte. Moreover, the electrode maintains the capacitance retention of 90.8% after 10000 chargingdischarging cycles at 1.0 A/g, exhibiting long cycling life. This study highlights a new avenue towards IPNs-derived carbons with unique pore structure for promising electrochemical applications.
基金the National Natural Science Foundation of China(No.81741090)the Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talentsthe National KePy R&D Program of China(Nos.2017YFC1001703 and 2018YFC1002700)。
文摘The pathogenesis of very-long-chain acyl-CoA dehydrogenase(VLCAD)deficiency is highly heterogeneous and still unclear.Additional novel variants have been recently detected in the population.The molecular and cellular effects of these previously unreported variants are still poorly understood and require further characterization.To address this problem,we have evaluated the various functions and biochemical consequences of six novel missense variants that lead to mild VLCAD deficiency.Marked deficiencies in fatty acid oxidation(FAO)and other mitochondrial defects were observed in cells carrying one of these six variants(c.541 C>T,c.863 T>G,c.895 A>G,c.1238 T>C,c.1276 G>A,and c.1505 T>A),including reductions in mitochondrial respiratory-chain function and adenosine teriphosphate(ATP)production,and increased levels of mitochondrial reactive oxygen species(ROS).Intriguingly,higher apoptosis levels were found in cells carrying the mutant VLCAD under glucose-limited stress.Moreover,the stability of the mutant homodimer was disturbed,and major conformational changes in each mutant VLCAD structure were predicted by molecular dynamics(MD)simulation.The data presented here may provide valuable information for improving management of diagnosis and treatment of VLCAD deficiency and for a better understanding of the general molecular bases of disease variability.
基金supported by National Science and Technology Major Project granted No.2016ZX01012101
文摘With the introduction of software defined hardware by DARPA Electronics Resurgence Initiative,software definition will be the basic attribute of information system.Benefiting from boundary certainty and algorithm aggregation of domain applications,domain-oriented computing architecture has become the technical direction that considers the high flexibility and efficiency of information system.Aiming at the characteristics of data-intensive computing in different scenarios such as Internet of Things(IoT),big data,artificial intelligence(AI),this paper presents a domain-oriented software defined computing architecture,discusses the hierarchical interconnection structure,hybrid granularity computing element and its computational kernel extraction method,finally proves the flexibility and high efficiency of this architecture by experimental comparison.
基金The auth ors gratefully ack no wledge the fi nan cial supp ort of the"Nati onal Key R&D Program of China"(2019YFD1000605-1)"The National Natural Science Foun dati on of Ch ina"(31922058)+3 种基金"Outsta nding Young Tale nt Fund in Beij ing Forestry University"(2019JQ03009)"The National Natural Science Foundation of China"(31800509),(31901281),(31930076)"The 111 Project"(B20088)"Heilongjiang Touyan Innovation Team Program"(Tree Genetics and Breeding Innovation Team).
文摘Mature pollen germinates rapidly on the stigma,extending its pollen tube to deliver sperm cells to the ovule for fertilization.The success of this process is an important factor that limits output.The flavonoid content increased signi ficantly during pollen germination and pollen tube growth,which suggests it may play an important role in these processes.However,the speci fi c mechanism of this involvement has been little researched.Our previous research found that hyperoside can prolong the flowering period of Abelmoschus esculentus(okra),but its speci fic mechanism is still unclear.Therefore,in this study,we focused on the effect of hyperoside in regulating the actin-depolymerizing factor(ADF),which further affects the germination and growth of pollen.We found that hyperoside can prolong the effective pollination period of okra by 2-3-fold and promote the growth of pollen tubes in the style.Then,we used Nicotiana benthamiana cells as a research system and found that hyperoside accelerates the depolymerization of intercellular micro fi laments.Hyperoside can promote pollen germination and pollen tube elongation in vitro.Moreover,AeADFl was identi fied out of all AeADF genes as being highly expressed in pollen tubes in response to hyperoside.In addition,hyperoside promoted AeADF1-mediated micro filament dissipation according to micro filament severing experiments in vitro.In the pollen tube,the gene expression of AeADFl was reduced to 1/5 by oligonucleotide transfection.The decrease in the expression level of AeADFl partially reduced the promoting effect of hyperoside on pollen germination and pollen tube growth.This research provides new research directions for flavonoids in reproductive development.
基金supported in part by the National Key R&D Program of China under Grant 2022YFB3104503in part by the China Postdoctoral Science Foundation under Grant 2024M750199+1 种基金in part by the National Natural Science Foundation of China under Grant 62202054,Grant 62002022 and Grant 62472251in part by the Fundamental Research Funds for the Central Universities under Grant BLX202360.
文摘This paper investigates an unmanned aerial vehicle(UAV)-assisted multi-object offloading scheme for blockchain-enabled Vehicle-to-Everything(V2X)systems.Due to the presence of an eavesdropper(Eve),the system’s com-munication links may be insecure.This paper proposes deploying an intelligent reflecting surface(IRS)on the UAV to enhance the communication performance of mobile vehicles,improve system flexibility,and alleviate eavesdropping on communication links.The links for uploading task data from vehicles to a base station(BS)are protected by IRS-assisted physical layer security(PLS).Upon receiving task data,the computing resources provided by the edge computing servers(MEC)are allocated to vehicles for task execution.Existing blockchain-based computation offloading schemes typically focus on improving network performance,such as minimizing energy consumption or latency,while neglecting the Gas fees for computation offloading and the costs required for MEC computation,leading to an imbalance between service fees and resource allocation.This paper uses a utility-oriented computation offloading scheme to balance costs and resources.This paper proposes alternating phase optimization and power optimization to optimize the energy consumption,latency,and communication secrecy rate,thereby maximizing the weighted total utility of the system.Simulation results demonstrate a notable enhancement in the weighted total system utility and resource utilization,thereby corroborating the viability of our approach for practical applications.
基金supported in part by the National Key R&D Program of China under Grant 2022YFB3104503in part by the National Natural Science Foundation of China under Grant 62202054,and Grant 61931001+2 种基金in part by the National Natural Science Foundation of China No.62202054the Young Elite Scientists Sponsorship Program of the China Association for Science and Technology under Grant 2023QNRC001in part by the U.S.National Science Foundation under Grant 2136202.
文摘Recognized as a pivotal facet in Beyond Fifth-Generation(B5G)and the upcoming Sixth-Generation(6G)wireless networks,Unmanned Aerial Vehicle(UAV)communications pose challenges due to limited capabilities when serving as mobile base stations,leading to suboptimal service for edge users.To address this,the collaborative formation of Coordinated Multi-Point(CoMP)networks proves instrumental in alleviating the issue of the poor Quality of Service(QoS)at edge users in the network periphery.This paper introduces a groundbreaking solution,the Hybrid Uplink-Downlink Non-Orthogonal Multiple Access(HUD-NOMA)scheme for UAV-aided CoMP networks.Leveraging network coding and NOMA technology,our proposed HUD-NOMA effectively enhances transmission rates for edge users,notwithstanding a minor reduction in signal reception reliability for strong signals.Importantly,the system’s overall sum rate is elevated.The proposed HUD-NOMA demonstrates resilience against eavesdroppers by effectively managing intended interferences without the need for additional artificial noise injection.The study employs a stochastic geometry approach to derive the Secrecy Outage Probability(SOP)for the transmissions in the CoMP network,revealing superior performance in transmission rates and lower SOP compared to existing methods through numerical verification.Furthermore,guided by the theoretical SOP derivation,this paper proposes a power allocation strategy to further reduce the system’s SOP.
基金supported by the Biological Breeding-Major Projects in National Science and Technology[grant numbers 2023ZD04044]the Natural Science Foundation of China Program[grant numbers 32072714].
文摘Background Intramuscular fat is an important factor in evaluating pork quality and varies widely among different pig breeds.However,the regulatory mechanism of circular RNAs(circRNAs)in lipid metabolism remains largely unexplored.Results We combined circRNA-seq and Ribo-seq data to screen a total of 18 circRNA candidates with coding potential,and circANKRD17 was found to be significantly elevated in the longissimus dorsi muscle of Lantang piglets,with a length of 1,844 nucleotides.Using single-cell sequencing,we identified 477 differentially expressed genes in IMF cells between Lantang and Landrace piglets,with enrichment in the PPAR signaling pathway.These genes included FABP4,FABP5,CPT1A,and UBC,consistent with the high levels of acylcarnitines observed in the longissimus dorsi muscles of the Lantang breed,as determined by lipidomic analysis.Further in vitro and in vivo experiments indicated that circANKRD17 can regulate lipid metabolism through various mechanisms involving the PPAR pathway,including promoting adipocyte differentiation,fatty acid transport and metabolism,triglyceride synthesis,and lipid droplet formation and maturation.In addition,we discovered that circANKRD17 has an open reading frame and can be translated into a novel 571-amino-acid protein that promotes lipid metabolism.Conclusions Our research provides new insights into the role of protein-coding circANKRD17,especially concerning the metabolic characteristics of pig breeds with higher intramuscular fat content.
文摘BACKGROUND Owing to the absence of specific symptoms in early-stage gastric cancer,most patients are diagnosed at intermediate or advanced stages.As a result,treatment often shifts from surgery to other therapies,with chemotherapy and targeted therapies being the primary options for advanced gastric cancer treatment.A total of 116 patients with advanced gastric cancer,admitted from January 2021 to December 2023,were selected and divided into two groups of 58 each using the random number table method.The control group received FOLFOX4 chemothe-rapy(oxaliplatin+calcium+folinate+5-fluorouracil)combined with intravenous sindilizumab.The observation group received the same treatment as the control group,supplemented by oral administration of Senqi Shiyiwei granules.Both groups underwent treatment cycles of 3 weeks,with a minimum of two cycles.The therapeutic efficacy,immune mechanisms,and treatment-related toxicity and side effects were compared between the groups.The objective remission rate in the observation group(55.17%)was higher than that of the control group(36.21%)(P<0.05).After two treatment cycle,CD3+,CD4+,and CD4+/CD8+levels were higher in the observation group compared to the control group,while CD8+,regulatory T cells,and natural killer cells were lower(P<0.05).Additionally,the incidence of leukopenia,nausea,and vomiting was lower in observed group(P<0.05).No significant differences were observed in the incidence of other adverse reactions(P>0.05).CONCLUSION Adjuvant therapy with Shenqixian granules may enhance the efficacy of simudizumab combined with FOLFOX4 chemotherapy in advanced gastric cancer and the immune function by increasing immune cell counts,making it a valuable option in clinical treatment.
文摘目的探讨单侧上尿路梗阻解除前后肾皮质厚度(RCT)与肾小球滤过率(GFR)的关系。方法选取2015年7月-2016年6月该院泌尿外科收治的63例上泌尿道梗阻患者为研究对象。其中,男34例,女29例,年龄22~73(52.21±11.56)岁;手术方式:输尿管镜碎石+取石术24例,一期经皮肾造瘘+二期经皮肾镜取石术(PCNL)21例,一期PCNL 18例。采用腹部增强CT、单光子发射计算机断层(SPECT)扫描分别测量RCT和GFR,根据术前患肾GFR情况分为3组:A组(中度肾损害,15.0 ml/min≤GFR<30.0 ml/min)、B组(重度肾损害,7.5 ml/min≤GFR<15.0 ml/min)、C组(极重度肾损害,GFR<7.5 ml/min),比较3组的手术前后的GFR变化。采用简单线性相关分析A和B组患者术前RCT与各指标的相关性。结果 9例患者因肾积水严重未测量RCT,患者的术前RCT>10 mm 20例,5~10 mm 23例,<5 mm 11例,平均值为(10.75±4.91)mm。A组术前RCT明显高于B组,差异有统计学意义(P<0.05)。3组患者术后1和9个月的GFR均明显高于同组术前,差异均有统计学意义(P<0.05)。A组术后1和9个月的GFR均明显高于另外两组,B组术后9个月的GFR明显高于C组,差异均有统计学意义(P<0.05),但术后1个月B组和C组的GFR差异无统计学意义(P>0.05)。B组术后1个月的GFR恢复值明显低于另外两组,差异有统计学意义(P<0.05),3组术后9个月的GFR恢复值比较差异无统计学意义(P>0.05)。术前RCT与术前GFR(r=0.613)、术后1个月GFR(r=0.697)及恢复值(r=0.552)、术后9个月GFR(r=0.589)及恢复值(r=0.488)均呈显著正相关,差异有统计学意义(P<0.05)。结论解除上泌尿道梗阻后患者的肾功能得到不同程度的恢复,即使是极重度肾功能损伤者,多数均能挽回患肾。术前RCT能有助于判断围手术期的肾功能,尤其是术后肾功能恢复情况。
