Periodontitis is the leading cause of tooth loss in adults.Unfortunately,inflammation remains poorly controlled and prone to relapse,even after removing the initial plaque biofilm.The unique metabolic properties of mi...Periodontitis is the leading cause of tooth loss in adults.Unfortunately,inflammation remains poorly controlled and prone to relapse,even after removing the initial plaque biofilm.The unique metabolic properties of mitochondria in the periodontal microenvironment provide a promising target for novel therapeutic strategies against periodontitis.Here,we integrate meta-bolomics and network biology to elucidate the potential role of nuclear factor E2-related factor 2/mitochondrial transcription factor(Nrf2/TFAM)in regulating mitochondrial metabolism in periodontitis.Based on this discovery,it is crucial to develop an innovative nanomedicine capable of effectively modulating the mitochondrial metabolism in periodontitis.Recently,itaconate(ITA),a key metabolite linking mitochondrial metabolism and inflammation,has emerged as a powerhouse in regulating immunity through Nrf2;however,its limited permeability hinders its application in biological systems.Therefore,we synthesize ITA-based nano cocktail(INC)with cell permeability and improved biological functions.At the cellular level,INC activates Nrf2/TFAM to remodel mitochondrial metabolism and regulate macrophage immune homeostasis.In mouse models of peri-odontitis,INC successfully reprograms mitochondrial metabolism within the gingiva,leading to an improved inflammatory microenvironment.Our study elucidates the role of INC in modulating mitochondrial metabolism,thereby offering an inno-vative therapeutic strategy for the management of periodontitis and other clinical conditions resulting from mitochondrial abnormalities.展开更多
Achieving strong coupling between plasmonic oscillators can significantly modulate their intrinsic optical properties.Here,we report the direct observation of ultrafast plasmonic hot electron transfer from an Au grati...Achieving strong coupling between plasmonic oscillators can significantly modulate their intrinsic optical properties.Here,we report the direct observation of ultrafast plasmonic hot electron transfer from an Au grating array to an MoS_(2) monolayer in the strong coupling regime between localized surface plasmons(LSPs)and surface plasmon polaritons(SPPs).By means of femtosecond pump-probe spectroscopy,the measured hot electron transfer time is approximately 40 fs with a maximum external quantum yield of 1.65%.Our results suggest that strong coupling between LSPs and SPPs has synergetic effects on the generation of plasmonic hot carriers,where SPPs with a unique nonradiative feature can act as an‘energy recycle bin’to reuse the radiative energy of LSPs and contribute to hot carrier generation.Coherent energy exchange between plasmonic modes in the strong coupling regime can further enhance the vertical electric field and promote the transfer of hot electrons between the Au grating and the MoS_(2) monolayer.Our proposed plasmonic strong coupling configuration overcomes the challenge associated with utilizing hot carriers and is instructive in terms of improving the performance of plasmonic opto-electronic devices.展开更多
Histopathological diagnosis is a crucial part of surgical treatment,as it determines further treatment decisions and prognosis for patients.Due to the heterogeneity of tumors,traditional histopathological diagnosis re...Histopathological diagnosis is a crucial part of surgical treatment,as it determines further treatment decisions and prognosis for patients.Due to the heterogeneity of tumors,traditional histopathological diagnosis relies on histological and cytological morphological analysis of cryo-slides by well-trained pathologists,which is subjective,time-consuming,and lacks considerable reliability.Therefore,we engineer a series of tumor receptor-seeking dyes to automatically label and visually depict the tumor contour with antibody-free interaction.The meso-chlorine on the cyclohexyl ring of dyes can covalently react with specific thiol groups in various tumor signature proteins to form stable dye@protein complexes.These dye@protein complexes have significantly amplified fluorescence signals in tumor sites,enabling highresolution delineation of tumormargins and providing histological morphology information to potentially determine tumor staging.The orthogonal effect not only avoids errors caused by individual signature proteins,but also amplifies the fluorescence difference between tumor and normal tissues.Notably,our method is easy to operate with a minimal detection time of only 4 min.The tumor receptor-seeking dyes successfully assess tumor-positive margins from 66 resected breast tumor samples and 25 intraoperative cancer patients with 100%accuracy,providing a newgeneration technique for intraoperative pathologic diagnosis of tumor biopsy.展开更多
基金supported by the National Natural Science Foundation of China(82301131)the Jilin Province Development and Reform Commission(2023C041-3)+1 种基金the Science,Technology Project of Jilin Provincial Department of Finance(JCSZ2023481-14)the Bethune Project of Jilin University(2023B27).
文摘Periodontitis is the leading cause of tooth loss in adults.Unfortunately,inflammation remains poorly controlled and prone to relapse,even after removing the initial plaque biofilm.The unique metabolic properties of mitochondria in the periodontal microenvironment provide a promising target for novel therapeutic strategies against periodontitis.Here,we integrate meta-bolomics and network biology to elucidate the potential role of nuclear factor E2-related factor 2/mitochondrial transcription factor(Nrf2/TFAM)in regulating mitochondrial metabolism in periodontitis.Based on this discovery,it is crucial to develop an innovative nanomedicine capable of effectively modulating the mitochondrial metabolism in periodontitis.Recently,itaconate(ITA),a key metabolite linking mitochondrial metabolism and inflammation,has emerged as a powerhouse in regulating immunity through Nrf2;however,its limited permeability hinders its application in biological systems.Therefore,we synthesize ITA-based nano cocktail(INC)with cell permeability and improved biological functions.At the cellular level,INC activates Nrf2/TFAM to remodel mitochondrial metabolism and regulate macrophage immune homeostasis.In mouse models of peri-odontitis,INC successfully reprograms mitochondrial metabolism within the gingiva,leading to an improved inflammatory microenvironment.Our study elucidates the role of INC in modulating mitochondrial metabolism,thereby offering an inno-vative therapeutic strategy for the management of periodontitis and other clinical conditions resulting from mitochondrial abnormalities.
基金supported by the National Key Research and Development Program of China(Grant No.2017YFA0205700)National Basic Research Program of China(Grant Nos.2015CB932403,2017YFA0206000)+4 种基金National Science Foundation of China(Grant Nos.11674012,61422501,11374023,61521004 and 21790364)Beijing Natural Science Foundation(Grant No.L140007)Foundation for the Author of National Excellent Doctoral Dissertation of PR China(Grant No.201420)National Program for Support of Top-notch Young Professionals(Grant No.W02070003)Ministry of Education Singapore under Grant No.MOE2015-T2-2-043.
文摘Achieving strong coupling between plasmonic oscillators can significantly modulate their intrinsic optical properties.Here,we report the direct observation of ultrafast plasmonic hot electron transfer from an Au grating array to an MoS_(2) monolayer in the strong coupling regime between localized surface plasmons(LSPs)and surface plasmon polaritons(SPPs).By means of femtosecond pump-probe spectroscopy,the measured hot electron transfer time is approximately 40 fs with a maximum external quantum yield of 1.65%.Our results suggest that strong coupling between LSPs and SPPs has synergetic effects on the generation of plasmonic hot carriers,where SPPs with a unique nonradiative feature can act as an‘energy recycle bin’to reuse the radiative energy of LSPs and contribute to hot carrier generation.Coherent energy exchange between plasmonic modes in the strong coupling regime can further enhance the vertical electric field and promote the transfer of hot electrons between the Au grating and the MoS_(2) monolayer.Our proposed plasmonic strong coupling configuration overcomes the challenge associated with utilizing hot carriers and is instructive in terms of improving the performance of plasmonic opto-electronic devices.
基金the National Key Research and Development Program of China(grant no.2022YFC2601900).
文摘Histopathological diagnosis is a crucial part of surgical treatment,as it determines further treatment decisions and prognosis for patients.Due to the heterogeneity of tumors,traditional histopathological diagnosis relies on histological and cytological morphological analysis of cryo-slides by well-trained pathologists,which is subjective,time-consuming,and lacks considerable reliability.Therefore,we engineer a series of tumor receptor-seeking dyes to automatically label and visually depict the tumor contour with antibody-free interaction.The meso-chlorine on the cyclohexyl ring of dyes can covalently react with specific thiol groups in various tumor signature proteins to form stable dye@protein complexes.These dye@protein complexes have significantly amplified fluorescence signals in tumor sites,enabling highresolution delineation of tumormargins and providing histological morphology information to potentially determine tumor staging.The orthogonal effect not only avoids errors caused by individual signature proteins,but also amplifies the fluorescence difference between tumor and normal tissues.Notably,our method is easy to operate with a minimal detection time of only 4 min.The tumor receptor-seeking dyes successfully assess tumor-positive margins from 66 resected breast tumor samples and 25 intraoperative cancer patients with 100%accuracy,providing a newgeneration technique for intraoperative pathologic diagnosis of tumor biopsy.
