The COVID-19 pandemic highlighted the urgent need to control airborne virus transmission,particularly in indoor environments with limited ventilation.This study evaluates the effectiveness of UVA and UVC irradiation,a...The COVID-19 pandemic highlighted the urgent need to control airborne virus transmission,particularly in indoor environments with limited ventilation.This study evaluates the effectiveness of UVA and UVC irradiation,along with hydrogen peroxide(H_(2)O_(2)),in inactivating aerosolized viruses.A 19 m^(3)virus aerosol simulation chamber,replicating indoor conditions,was used to simulate human respiratory emissions by aerosolizing Escherichia phage T4(T4 phages)embedded in a pig mucin medium that mimics respiratory aerosols.Results showed a clear,dose-dependent reduction in viral genome copies with UVC exposure,where a dose of 129.9 mJ/cm^(2)reduced over 99%of the viral genome copies.Although less efficient,UVA still contributed to virus inactivation,reducing detectable phages to 20%at 513.30 J/cm^(2).Mucin provided a protective effect,making virus removal more challenging.Hydrogen peroxide enhanced disinfection,with 1.6 ppm reducing viral genome copies by 78%,and higher concentrations(up to 16 ppm)achieving over 99%reduction in the dark condition.The combination of UVA/UVC with H_(2)O_(2)further enhanced disinfection,eliminating detectable virus genome copies entirely.These findings underscore the potential for using combined UV light and chemical treatments to effectively mitigate airborne viral transmission in enclosed spaces.展开更多
基金supported by the Federal Ministry of Education and Research(BMBF)under project number 13GW0597E(BeCoLe main project,with subprojects KAPAVIR and MikroVirasol)the Deutsche Forschungsgemeinschaft(DFG,German Research Foundation)under project number 468717405(AEROVIR)supported by European Regional Development Funds(EFRE)and the Helmholtz Association for using its microanalytical facilities.
文摘The COVID-19 pandemic highlighted the urgent need to control airborne virus transmission,particularly in indoor environments with limited ventilation.This study evaluates the effectiveness of UVA and UVC irradiation,along with hydrogen peroxide(H_(2)O_(2)),in inactivating aerosolized viruses.A 19 m^(3)virus aerosol simulation chamber,replicating indoor conditions,was used to simulate human respiratory emissions by aerosolizing Escherichia phage T4(T4 phages)embedded in a pig mucin medium that mimics respiratory aerosols.Results showed a clear,dose-dependent reduction in viral genome copies with UVC exposure,where a dose of 129.9 mJ/cm^(2)reduced over 99%of the viral genome copies.Although less efficient,UVA still contributed to virus inactivation,reducing detectable phages to 20%at 513.30 J/cm^(2).Mucin provided a protective effect,making virus removal more challenging.Hydrogen peroxide enhanced disinfection,with 1.6 ppm reducing viral genome copies by 78%,and higher concentrations(up to 16 ppm)achieving over 99%reduction in the dark condition.The combination of UVA/UVC with H_(2)O_(2)further enhanced disinfection,eliminating detectable virus genome copies entirely.These findings underscore the potential for using combined UV light and chemical treatments to effectively mitigate airborne viral transmission in enclosed spaces.
