Objective:To investigate the effect of miR-483-5p on hepatocellular carcinoma(HCC)cells proliferation and stemness,as well as the attenuating effect of Pien Tze Huang(PZH).Methods:Differentially expressed miRNA betwee...Objective:To investigate the effect of miR-483-5p on hepatocellular carcinoma(HCC)cells proliferation and stemness,as well as the attenuating effect of Pien Tze Huang(PZH).Methods:Differentially expressed miRNA between HepG2 cells and hepatic cancer stem-like cells(HCSCs)were identified by a miRNA microarray assay.miR-483-5p mimics were transfected into HepG2 cells to explore the effects of miR-483-5p on cell proliferation and stemness.HepG2 cells and HCSCs were treated with PZH(0,0.25,0.50 and 0.75 mg/mL)to explore the effects of PzH on the proliferation and stemness,both in non-induced state and the state induced by miR-483-5p mimics.Results:miR-483-5p was significantly up-regulated in HCSCs and its overexpression increased cell proliferation and stemness in HepG2 cells(P<0.05).PZH not only significantly inhibited proliferation in HepG2 cells,but also significantly suppressed the cell proliferation and self-renewal of HCSCs(P<0.05).The effects of miR-483-5p mimics on proliferation and stemness of HepG2 cells were partially abolished by PZH.Conclusions:miR-483-5p promotes proliferation and enhances stemness of HepG2 cells,which were attenuated by PZH,demonstrating that miR-483-5p is a potential molecular target for the treatment of HCC and provide experimental evidence to support clinical use of PZH for patients with HCC.展开更多
基金Supported by the National Natural Science Foundation of China(No.81673721)Natural Science Foundation of Fujian Province(No.2018J01352)Natural Science Foundation of Fujian University of Traditional Chinese Medicine(No.X2023016)。
文摘Objective:To investigate the effect of miR-483-5p on hepatocellular carcinoma(HCC)cells proliferation and stemness,as well as the attenuating effect of Pien Tze Huang(PZH).Methods:Differentially expressed miRNA between HepG2 cells and hepatic cancer stem-like cells(HCSCs)were identified by a miRNA microarray assay.miR-483-5p mimics were transfected into HepG2 cells to explore the effects of miR-483-5p on cell proliferation and stemness.HepG2 cells and HCSCs were treated with PZH(0,0.25,0.50 and 0.75 mg/mL)to explore the effects of PzH on the proliferation and stemness,both in non-induced state and the state induced by miR-483-5p mimics.Results:miR-483-5p was significantly up-regulated in HCSCs and its overexpression increased cell proliferation and stemness in HepG2 cells(P<0.05).PZH not only significantly inhibited proliferation in HepG2 cells,but also significantly suppressed the cell proliferation and self-renewal of HCSCs(P<0.05).The effects of miR-483-5p mimics on proliferation and stemness of HepG2 cells were partially abolished by PZH.Conclusions:miR-483-5p promotes proliferation and enhances stemness of HepG2 cells,which were attenuated by PZH,demonstrating that miR-483-5p is a potential molecular target for the treatment of HCC and provide experimental evidence to support clinical use of PZH for patients with HCC.
