Bioreactors are used to dynamically condition engineered tissues to achieve the required degree of maturation before in vivo implantation.Integrating sensors and imaging capabilities into bioreactors can help us under...Bioreactors are used to dynamically condition engineered tissues to achieve the required degree of maturation before in vivo implantation.Integrating sensors and imaging capabilities into bioreactors can help us understand how the culture environment influences tissue maturation and growth.Additionally,this enables the monitoring of tissue constructs and provides critical information for quality control.This study aimed to develop a standardized,self-contained,uniaxial bioreactor module for the clinical manufacturing of tissue constructs;this system would benefit from unidirectional mechanical or electrical stimulation,or both.We achieved this goal by integrating stimulation and sensing components that provide an optimal culture environment and monitoring capabilities to improve tissue manufacturing.The uniaxial bioreactor module included integrated,user-friendly mechanical and electrical stimulations with force measurement to enhance the preconditioning of the engineered tissues.Also,a sensor loop and media exchange system were integrated to monitor the culture environment and cellular metabolites over time,and the camera system above the tissue construct enabled the macroscopic visualization of tissue maturation.Furthermore,the onboard media exchange system was programmed into the module to maintain aseptic culture conditions in the long term.Subsequently,using native skeletal muscle tissue and tissue-engineered skeletal muscle constructs,the performance of the uniaxial bioreactor module was validated for its application in preconditioning and enhancing tissue maturation.展开更多
The fields of regenerative medicine and tissue engineering offer new therapeutic options to restore,maintain or improve tissue function following disease or injury.To maximize the biological function of a tissue-engin...The fields of regenerative medicine and tissue engineering offer new therapeutic options to restore,maintain or improve tissue function following disease or injury.To maximize the biological function of a tissue-engineered clinical product,specific conditions must be maintained within a bioreactor to allow the maturation of the product in preparation for implantation.Specifically,the bioreactor should be designed to mimic the mechanical,electrochemical and biochemical environment that the product will be exposed to in vivo.Real-time monitoring of the functional capacity of tissue-engineered products during manufacturing is a critical component of the quality management process.The present review provides a brief overview of bioreactor engineering considerations.In addition,strategies for bioreactor automation,in-line product monitoring and quality assurance are discussed.展开更多
基金possible by the US Army Medical Research and Development Command through the Medical Technology Enterprise Consortium under Contract#W81XWH-15-9-0001.
文摘Bioreactors are used to dynamically condition engineered tissues to achieve the required degree of maturation before in vivo implantation.Integrating sensors and imaging capabilities into bioreactors can help us understand how the culture environment influences tissue maturation and growth.Additionally,this enables the monitoring of tissue constructs and provides critical information for quality control.This study aimed to develop a standardized,self-contained,uniaxial bioreactor module for the clinical manufacturing of tissue constructs;this system would benefit from unidirectional mechanical or electrical stimulation,or both.We achieved this goal by integrating stimulation and sensing components that provide an optimal culture environment and monitoring capabilities to improve tissue manufacturing.The uniaxial bioreactor module included integrated,user-friendly mechanical and electrical stimulations with force measurement to enhance the preconditioning of the engineered tissues.Also,a sensor loop and media exchange system were integrated to monitor the culture environment and cellular metabolites over time,and the camera system above the tissue construct enabled the macroscopic visualization of tissue maturation.Furthermore,the onboard media exchange system was programmed into the module to maintain aseptic culture conditions in the long term.Subsequently,using native skeletal muscle tissue and tissue-engineered skeletal muscle constructs,the performance of the uniaxial bioreactor module was validated for its application in preconditioning and enhancing tissue maturation.
基金US Army Medical Research and Development Command through the Medical Technology Enterprise Consortium under Contract#W81XWH-15-9-0001.
文摘The fields of regenerative medicine and tissue engineering offer new therapeutic options to restore,maintain or improve tissue function following disease or injury.To maximize the biological function of a tissue-engineered clinical product,specific conditions must be maintained within a bioreactor to allow the maturation of the product in preparation for implantation.Specifically,the bioreactor should be designed to mimic the mechanical,electrochemical and biochemical environment that the product will be exposed to in vivo.Real-time monitoring of the functional capacity of tissue-engineered products during manufacturing is a critical component of the quality management process.The present review provides a brief overview of bioreactor engineering considerations.In addition,strategies for bioreactor automation,in-line product monitoring and quality assurance are discussed.
