CorelKit is developed with the built-in VBA environment of CorelDraw?,and is a new plug-in in CorelDraw for geological and geochemical drawing.CorelKit can help users quickly and easily draw geological and geochemical...CorelKit is developed with the built-in VBA environment of CorelDraw?,and is a new plug-in in CorelDraw for geological and geochemical drawing.CorelKit can help users quickly and easily draw geological and geochemical graphs,such as scatterplot,triangular scatterplot,line chart,histogram,bar chart,box plot,pie graph,etc.In order to adapt to the geological application,the above functions are strengthened.Scatterplot and triangular scatterplot provide nearly 150 common basemaps,covering rock classification,structural environment discrimination,mineral genesis,isotope geochemistry,etc.,for the convenience of the user mapping.Meanwhile,CorelKit further provides a structural joint rose diagram and sulfur isotopic composition diagram.This software also has many practical functions such as Bézier line topology,Multiple Bézier lines close fill,Single inner fill,Correction basemap,Add a compass,Add a scale,which facilitates daily geological drawing.The software has a friendly interface,in both Chinese and English versions,and is suitable for CorelDraw X4 and later versions.We introduced the Bézier line topology to CorelKit for the first time,which is similar to the GIS®topology for geological maps.According to the“GB/T 958-2015 Geological Legends Used for Regional Geological Map”,a two-color filling library of rock pattern pictures is established,which is convenient to realize the twocolor filling of sedimentary rocks,metamorphic rocks and magmatic rocks patterns.Obviously,CorelKit makes up for many shortcomings of CorelDraw in geosciences and is a very practical tool for geoscience researchers.展开更多
Background:Electroacupuncture is often used to treat insomnia.Objective:To evaluate the efficacy and safety of electroacupuncture for insomnia.Search strategy:Databases including PubMed,Cochrane Library,Embase,Web of ...Background:Electroacupuncture is often used to treat insomnia.Objective:To evaluate the efficacy and safety of electroacupuncture for insomnia.Search strategy:Databases including PubMed,Cochrane Library,Embase,Web of Science,Chinese Biomedical Literature Database,China National Knowledge Infrastructure,Wanfang Data and VIP Fulltext e-Journals Database were searched up to January 15,2023.Inclusion criteria:Randomized clinical trials were included if they compared the clinical efficacy and safety of electroacupuncture with sham acupuncture,no treatment or usual care (UC) and general acupuncture.Data extraction and analysis:The full texts of the studies were reviewed to remove ineligible literature.The extracted data included authors,publication year,diagnostic criteria,sample size,population characteristics,interventions and outcomes.The above steps were performed independently by two reviewers and the data were cross checked.Stata15.0 software was used to analyze the extracted outcome data.For continuous data (Pittsburgh Sleep Quality Index[PSQI]score and Insomnia Severity Index score),weighted mean difference (WMD) was calculated and 95% confidence interval (CI) was reported when the same scale was applied.For dichotomous variables (clinical response rate and adverse events),a meta-analysis was performed using risk ratio (RR) as the effect indicator.Results:Thirty-one trials with 2226 subjects were included.The meta-analysis suggested that electroacupuncture was more effective in improving insomnia compared with the control group (sham acupuncture,no treatment,UC and general acupuncture)(RR=1.21;95 CI:[1.16,1.27]),significantly reducing the PSQI score in insomnia patients after treatment and at follow-up (WMD=-3.23;95%CI:[-4.29,-2.17];P<0.001).There was no significant difference in the incidence of adverse events between the EA and control groups (sham acupuncture and no treatment or UC.RR=1.48;95%CI:[0.91,2.40];P=0.117).In addition,the regression results revealed that receiving electroacupuncture for seven to nine weeks provided the best efficacy (P<0.05). Conclusion:Electroacupuncture can significantly promote better sleep quality in insomnia patients and is suitable for the treatment of various types of insomnia.However,the articles included were single-center trials with small sample sizes,and some articles were of poor quality.Therefore,further research is still needed to confirm these findings.展开更多
Since the discovery of adipose-derived mesenchymal stem cell (ADSC) in more than ten years, a great progress has been made from its basic research to clinical application. Compared with bone marrow mesenchymal stem ce...Since the discovery of adipose-derived mesenchymal stem cell (ADSC) in more than ten years, a great progress has been made from its basic research to clinical application. Compared with bone marrow mesenchymal stem cells, ADSC is more abundant in reserve, easier to obtain with fewer injuries and less complications. These cells have multiple differentiation potential and can differentiate into adipocytes, chondrocytes and osteoblasts with the influence of different inducing factors. Early studies of ADSC mainly focused on the ability of multi-directional differentiation, especially on the regeneration of bone defects and cartilage tissue. At present, the researches mainly focus on immunoregulation and paracrine function of ADSC. Although ADSC has made a great progress in clinical application, the cell preparation, use pattern, and mechanisms in clinical treatment are not clear. This paper elaborates on these issues.展开更多
Objective:To explore the burn treatment and first aid flow mode in batch patients.Methods:A retrospective analysis was performed on the treatment experience of 10 or more patients in the group burn accident admitted t...Objective:To explore the burn treatment and first aid flow mode in batch patients.Methods:A retrospective analysis was performed on the treatment experience of 10 or more patients in the group burn accident admitted to the Third Affiliated Hospital of Inner Mongolia Medical University from 2008 to 2020,in order to acquire the general information(e.g.,age,sex[male and female],injury causes and factors,total burn surface area,inhalation injury and compound injury),the time when each first-aid rescue team was in place;the reception time of the triage group;specialist rescue start time;treatment process and treatment outcome in patients with severe burns,such as the establishment time of deep venous access.Results:67 cases(n=68)of patients were cured and the curative ratio was 98.5%.The average time from the rescue order of the commander-in-chief to the placement of each medical group was 8.8 minutes,the average reception time of the triage group was 4.5 minutes,the average specialist rescue start time was 18.6 minutes,and the average establishment time of deep vein access for patients with severe burns was about 11.3 minutes.Conclusions:Scientific and reasonable pre-hospital emergency triage and treatment for burn patients can be achieved by implementing integrated treatment.展开更多
The increasing incidence of melanoma poses significant challenges for conventional treatment approaches,plagued by drug resistance and adverse side effects.Natural marine-derived compounds have gained prominence in me...The increasing incidence of melanoma poses significant challenges for conventional treatment approaches,plagued by drug resistance and adverse side effects.Natural marine-derived compounds have gained prominence in melanoma research for their unique bioactivities and diversity.This review delves into the therapeutic potential of these compounds in melanoma treatment,emphasizing their distinctive advantages such as multi-target mechanisms and immune modulation,which distinguish them from traditional therapies.Additionally,we discuss the challenges in translating these agents into clinical applications,including formulation stability,bioavailability,and regulatory hurdles.Recent advancements in preclinical models such as organoids and completed clinical trials further support the exploration of marine-derived compounds in melanoma management.By consolidating current research,this review underscores the potential of these agents to enhance treatment efficacy and foster new therapeutic strategies.展开更多
Objective:To observe the effects of platelet-rich plasma(PRP)on in vitro proliferation and migration of fibroblasts from human chronic refractory wound granulation tissue.Methods:Fibroblasts were separated from human ...Objective:To observe the effects of platelet-rich plasma(PRP)on in vitro proliferation and migration of fibroblasts from human chronic refractory wound granulation tissue.Methods:Fibroblasts were separated from human chronic refractory wound granulation tissue and then were identified.The obtained fibroblasts were divided into fetal bovine serum(FBS)group,hydrogel group and PRP group,and the three groups were cultured with culture mediums containing FBS,hydrogel and PRP respectively,in order to observe the growth of fibroblasts.The wound scratch assay was used to observe the migration of fibroblasts.Results:PRP group had more fibroblasts than FBS group and hydrogel group since Day 5 of culture,and exhibited greater fibroblast scratch migration area than FBS group on 48 h and 72 h of wound scratch assay(all p<.05).Conclusions:Compared with FBS,human fibroblasts cultured by PRP can more effectively promote the proliferation and migration of fibroblasts.展开更多
Burn as a strong traumatic stimulus can induce inflammatory responses mediated by neuroendocrine,cytokines and other inflammatory mediators,and directly lead to organ dysfunction,resulting in severe MODS.Excessive inf...Burn as a strong traumatic stimulus can induce inflammatory responses mediated by neuroendocrine,cytokines and other inflammatory mediators,and directly lead to organ dysfunction,resulting in severe MODS.Excessive inflammatory responses caused by necrotic tissues in the burned wound,also play an important role in stimulating the occurrence and the development of MODS.Complete removal of necrotic tissues in the early stage of burns to prevent from invasive wound infections,can effectively prevent MODS.AKI occurred earlier in this case,combined with its admission after the onset of shock and no obstruction of the urinary system can exclude prerenal and renal AKI,speculated as renal AKI.The factors leading to renal AKI are divided into renal ischemia and nephrotoxicity.The patient had adequate urinary output during shock and AKI in the presence of adequate renal blood flow,possibly due to the nephrotoxicity of a large number of necrotic tissues of the wound after they were broken down and absorbed into the bloodstream.The patient fell asleep after being drunk and the bedside stove broiled him for a long time,on one hand,heat accumulation lead to skin damage;on the other hand,sustained heat action caused hyperemia,edema and hemodynamic disorder in the deep tissues,and then resulted in secondary necrosis.展开更多
Background:Aphasia is one of the common complications of stroke.Speech and language therapy is a conventional treatment for aphasia following stroke.However,rehabilitation often remains unsatisfactory after speech and...Background:Aphasia is one of the common complications of stroke.Speech and language therapy is a conventional treatment for aphasia following stroke.However,rehabilitation often remains unsatisfactory after speech and language therapy alone.Acupuncture may be effective for aphasia after stroke.We designed this systematic review to assess the efficacy and safety of acupuncture as an adjunct to speech and language therapy for aphasia following ischemic stroke.Methods:An extensive search will be performed in databases including Cochrane Central Register of Controlled Trials,PubMed,Web of Science,EMBASE,China National Knowledge Infrastructure,Sinomed,Wei Pu Date and Wan Fang Data.No limitation will be set in language,publication date or status of the study.The primary outcomes include scores of Western Aphasia Battery and Aphasia Quotient.The secondary outcomes include Scales of Clinical Rehabilitation Research Center Aphasia Examination,Communicative Abilities in Daily Living,Aphasia Battery of Chinese,Chinese Functional Communication Profile,adverse reactions,etc.Results:All of the data will be processed with Review Manager software.Cochrane‘risk of bias’tool will be used to assess the risk of bias.Conclusion:This study will contribute to the selection of appropriate therapies for aphasia after ischemic stroke and the development of clinical guidelines on this topic.展开更多
Inflammation is generally considered as a pathological process,and the occurrence of multi-organ inflammatory diseases is usually accompanied by an inflammatory response.Persistent inflammation can eventually lead to ...Inflammation is generally considered as a pathological process,and the occurrence of multi-organ inflammatory diseases is usually accompanied by an inflammatory response.Persistent inflammation can eventually lead to organ dysfunction.In recent years,the study of mesenchymal stem cell-derived exosomes has found its unique anti-inflammatory effect,which provides a new idea for the treatment of multi-organ inflammatory diseases.This article reviews the effect of mesenchymal stem cell exosomes on multi-organ inflammatory diseases.展开更多
Objective: To explore the effects of combined application of culture supernatant of human umbilical cord mesenchymal stem cells (hUCMSCs) and ciprofloxacin on Staphylococcus aureus (SA) in vitro. Methods: hUCMSCs were...Objective: To explore the effects of combined application of culture supernatant of human umbilical cord mesenchymal stem cells (hUCMSCs) and ciprofloxacin on Staphylococcus aureus (SA) in vitro. Methods: hUCMSCs were isolated from umbilical cord tissues of full-term healthy fetuses after cesarean section and then cultured. Cells in the third passage were chosen for the use of experiment after identification. SA strains isolated from wounds of burn patients in our burn wards were used in the following experiment. Cells were divided into 0, 10, 100 and 1,000 ng/ml lipopolysaccharide (LPS) groups by use of the random number table (similarly hereinafter). Cells were cultured with culture medium containing mesenchymal stem cells (MSCs) after being treated with medium containing corresponding mass concen-trations of LPS for 12 h. At post culture hour (PCH) 6, 12 and 24, 6 wells of culture supernatant of cells in each group were obtained to measure the content of LL-37 with enzyme-linked immunosorbent assay (ELISA). Ninety blood agar culture plates were divided into ciprofloxacin control group (CC), ciprofloxacin + supernatant group (CS), and ciprofloxacin + supernatant+ LL-37 antibody group (CSL), with 30 blood agar culture plates in each group. Blood agar culture plates in group CC were coated with 1.5 × 108 colony forming unit (CFU)/ml bacteria solution prepared with normal saline. Blood agar culture plates in group CS were coated with 1.5 × 108 CFU/ml bacteria solution prepared with normal saline and hUCMSC culture supernatant (cultured by MSC culture medium, the same below) in double volume of normal saline. Blood agar culture plates in group CSL were coated with 1.5 × 108 CFU/ml bacteria solution prepared with normal saline, hUCMSC culture supernatant in double volume of normal saline, and 2.6 μL of LL-37 antibodies at the concentration of 2 μg/ml. At PCH 12, 24 and 48, 10 blood agar culture plates were taken out from each group to observe the distribution of SA colonies on blood agar culture plates and to measure diameters of zones of inhibition of ciprofloxacin. The minimum inhibitory concentration (MIC) of ciprofloxacin against SA in each group was recorded. Fractional inhibitory concentration (FIC) indexes of ciprofloxacin in group CS and group CSL at PCH 12, 24 and 48 were calculated, with the synergistic effect evaluated. Data were processed with factorial design ANOVA, one way ANOVA, LSD-t test, Kruskal-Wallis test and Mann-Whitney U test. Results: (1) At each PCH, the content of LL-37 in cell culture supernatant in 10 ng/ml LPS group, 100 ng/ml LPS group or 1,000 ng/ml LPS group was higher than that in 0 ng/ml LPS group (with t values ranging from 11.22 to 33.36, p values all below .01);the content of LL-37 in cell culture supernatant in either 100 ng/ml LPS group or 1,000 ng/ml LPS group was higher than that in 10 ng/ml LPS group (with t values ranging from 2.24 to 18.73, p < .05 or p < .01);the content of LL-37 in cell culture supernatant in 1,000 ng/ml LPS group was higher than that in 100 ng/ml LPS group (with t values ranging from 12.46 to 14.70, p values all below .01). (2) At PCH 12, 24 and 48, the bacterial colonies in groups CC, CS and CSL became integrated over time. In CC group, diameters of zones of inhibition of ciprofloxacin at PCH 12, 24 and 48 were 26 mm, 24 mm and 23 mm respectively, with no obvious changes. At PCH 12, 24 and 48, diameters of zones of inhibition of ciprofloxacin in groups CS and CSL were 82 mm, 71 mm, 68 mm and 74 mm, 59 mm, 56 mm respectively, which were significantly larger than those in group CC. (3) At each PCH, MIC of ciprofloxacin against SA in group CC was significantly higher than that in groups CS and CSL respectively (with Z values ranging from 6.22 to 6.71, p values all below .01);MIC of ciprofloxacin against SA in group CSL was significantly higher than that in group CS (with Z values all equal to 6.72, p values all below .01). (4) FIC indexes of ciprofloxacin in groups CS and CSL at PCH 12, 24 and 48 were 0.011, 0.032, 0.032 and 0.122, 0.350, 0.350, respectively. The results indicated that hUCMSC culture supernatant had a synergistically antibacterial effect when combined with ciprofloxacin. Conclusions: hUCMSCs can secrete LL-37, and the secretion level is improved with increase of LPS concentration. The com-bination of hUCMSC culture supernatant with ciprofloxacin can decrease the dosage of ciprofloxacin in resisting SA effectively. Once LL-37 is neutralized, the synergistically antibacterial effect of hUCMSC culture supernatant is decreased thereby.展开更多
Background and Aims:Liver imaging reporting and data system(LI-RADS)provides standardized lexicon and categorization for diagnosing hepatocellular carcinoma(HCC).However,there is limited knowledge about the effect of ...Background and Aims:Liver imaging reporting and data system(LI-RADS)provides standardized lexicon and categorization for diagnosing hepatocellular carcinoma(HCC).However,there is limited knowledge about the effect of LI-RADS training.We prospectively explored whether the systematic training of LI-RADS v2018 on magnetic resonance imaging(MRI)can effectively improve the diagnostic performances of different radiologists for HCC.Methods:A total of 20 visiting radiologists and the multiparametric MRI of 70 hepatic observations in 61 patients with high risk of HCC were included in this study.The LI-RADS v2018 training procedure included three times of thematic lectures(each lasting for 2.5 h)given by a professor specialized in imaging diagnosis of liver,with an interval of a month.After each seminar,the radiologists had a month to adopt the algorithm into their daily work.The diagnostic performances and interobserver agreements of these radiologists adopting the algorithm for HCC diagnosis before and after training were compared.Results:A total of 20 radiologists(male/female,12/8;with an average age of 36.75±4.99 years)were enrolled.After training,the interobserver agreements for the LI-RADS category for all radiologists(p=0.005)were increased.The sensitivity,specificity,positive predictive value,negative predictive value,and coincidence rate of all radiologists for HCC diagnosis before and after training were 43%vs.54%,86%vs.88%,74%vs.81%,62%vs.67%,and 65%vs.71%,respectively.The diagnostic performances of all radiologists(p<0.001)showed improvement after training.Conclusions:The systematic training of LI-RADS can effectively improve the diagnostic performances of radiologists with different experiences for HCC.展开更多
基金financially supported by the National Natural Science Foundation of China(Nos.91962218,42273061,41873034)the National Key Research and Development Plan(No.2022YFC2903503)the Fundamental Research Funds for the Central Universities of China(No.PA2019GDZC0093)。
文摘CorelKit is developed with the built-in VBA environment of CorelDraw?,and is a new plug-in in CorelDraw for geological and geochemical drawing.CorelKit can help users quickly and easily draw geological and geochemical graphs,such as scatterplot,triangular scatterplot,line chart,histogram,bar chart,box plot,pie graph,etc.In order to adapt to the geological application,the above functions are strengthened.Scatterplot and triangular scatterplot provide nearly 150 common basemaps,covering rock classification,structural environment discrimination,mineral genesis,isotope geochemistry,etc.,for the convenience of the user mapping.Meanwhile,CorelKit further provides a structural joint rose diagram and sulfur isotopic composition diagram.This software also has many practical functions such as Bézier line topology,Multiple Bézier lines close fill,Single inner fill,Correction basemap,Add a compass,Add a scale,which facilitates daily geological drawing.The software has a friendly interface,in both Chinese and English versions,and is suitable for CorelDraw X4 and later versions.We introduced the Bézier line topology to CorelKit for the first time,which is similar to the GIS®topology for geological maps.According to the“GB/T 958-2015 Geological Legends Used for Regional Geological Map”,a two-color filling library of rock pattern pictures is established,which is convenient to realize the twocolor filling of sedimentary rocks,metamorphic rocks and magmatic rocks patterns.Obviously,CorelKit makes up for many shortcomings of CorelDraw in geosciences and is a very practical tool for geoscience researchers.
文摘Background:Electroacupuncture is often used to treat insomnia.Objective:To evaluate the efficacy and safety of electroacupuncture for insomnia.Search strategy:Databases including PubMed,Cochrane Library,Embase,Web of Science,Chinese Biomedical Literature Database,China National Knowledge Infrastructure,Wanfang Data and VIP Fulltext e-Journals Database were searched up to January 15,2023.Inclusion criteria:Randomized clinical trials were included if they compared the clinical efficacy and safety of electroacupuncture with sham acupuncture,no treatment or usual care (UC) and general acupuncture.Data extraction and analysis:The full texts of the studies were reviewed to remove ineligible literature.The extracted data included authors,publication year,diagnostic criteria,sample size,population characteristics,interventions and outcomes.The above steps were performed independently by two reviewers and the data were cross checked.Stata15.0 software was used to analyze the extracted outcome data.For continuous data (Pittsburgh Sleep Quality Index[PSQI]score and Insomnia Severity Index score),weighted mean difference (WMD) was calculated and 95% confidence interval (CI) was reported when the same scale was applied.For dichotomous variables (clinical response rate and adverse events),a meta-analysis was performed using risk ratio (RR) as the effect indicator.Results:Thirty-one trials with 2226 subjects were included.The meta-analysis suggested that electroacupuncture was more effective in improving insomnia compared with the control group (sham acupuncture,no treatment,UC and general acupuncture)(RR=1.21;95 CI:[1.16,1.27]),significantly reducing the PSQI score in insomnia patients after treatment and at follow-up (WMD=-3.23;95%CI:[-4.29,-2.17];P<0.001).There was no significant difference in the incidence of adverse events between the EA and control groups (sham acupuncture and no treatment or UC.RR=1.48;95%CI:[0.91,2.40];P=0.117).In addition,the regression results revealed that receiving electroacupuncture for seven to nine weeks provided the best efficacy (P<0.05). Conclusion:Electroacupuncture can significantly promote better sleep quality in insomnia patients and is suitable for the treatment of various types of insomnia.However,the articles included were single-center trials with small sample sizes,and some articles were of poor quality.Therefore,further research is still needed to confirm these findings.
文摘Since the discovery of adipose-derived mesenchymal stem cell (ADSC) in more than ten years, a great progress has been made from its basic research to clinical application. Compared with bone marrow mesenchymal stem cells, ADSC is more abundant in reserve, easier to obtain with fewer injuries and less complications. These cells have multiple differentiation potential and can differentiate into adipocytes, chondrocytes and osteoblasts with the influence of different inducing factors. Early studies of ADSC mainly focused on the ability of multi-directional differentiation, especially on the regeneration of bone defects and cartilage tissue. At present, the researches mainly focus on immunoregulation and paracrine function of ADSC. Although ADSC has made a great progress in clinical application, the cell preparation, use pattern, and mechanisms in clinical treatment are not clear. This paper elaborates on these issues.
基金Health Research Project of Metallurgical Safety and Health Branch of The Chinese Society for Metals(JKWS201832)Natural Science Foundation of Inner Mongolia Autonomous Region(2020MS03022)+1 种基金Health Science and Technology Project of Inner Mongolia Autonomous Region(202201523)Joint Project of Inner Mongolia Medical University(YKD2021LH057).
文摘Objective:To explore the burn treatment and first aid flow mode in batch patients.Methods:A retrospective analysis was performed on the treatment experience of 10 or more patients in the group burn accident admitted to the Third Affiliated Hospital of Inner Mongolia Medical University from 2008 to 2020,in order to acquire the general information(e.g.,age,sex[male and female],injury causes and factors,total burn surface area,inhalation injury and compound injury),the time when each first-aid rescue team was in place;the reception time of the triage group;specialist rescue start time;treatment process and treatment outcome in patients with severe burns,such as the establishment time of deep venous access.Results:67 cases(n=68)of patients were cured and the curative ratio was 98.5%.The average time from the rescue order of the commander-in-chief to the placement of each medical group was 8.8 minutes,the average reception time of the triage group was 4.5 minutes,the average specialist rescue start time was 18.6 minutes,and the average establishment time of deep vein access for patients with severe burns was about 11.3 minutes.Conclusions:Scientific and reasonable pre-hospital emergency triage and treatment for burn patients can be achieved by implementing integrated treatment.
文摘The increasing incidence of melanoma poses significant challenges for conventional treatment approaches,plagued by drug resistance and adverse side effects.Natural marine-derived compounds have gained prominence in melanoma research for their unique bioactivities and diversity.This review delves into the therapeutic potential of these compounds in melanoma treatment,emphasizing their distinctive advantages such as multi-target mechanisms and immune modulation,which distinguish them from traditional therapies.Additionally,we discuss the challenges in translating these agents into clinical applications,including formulation stability,bioavailability,and regulatory hurdles.Recent advancements in preclinical models such as organoids and completed clinical trials further support the exploration of marine-derived compounds in melanoma management.By consolidating current research,this review underscores the potential of these agents to enhance treatment efficacy and foster new therapeutic strategies.
文摘Objective:To observe the effects of platelet-rich plasma(PRP)on in vitro proliferation and migration of fibroblasts from human chronic refractory wound granulation tissue.Methods:Fibroblasts were separated from human chronic refractory wound granulation tissue and then were identified.The obtained fibroblasts were divided into fetal bovine serum(FBS)group,hydrogel group and PRP group,and the three groups were cultured with culture mediums containing FBS,hydrogel and PRP respectively,in order to observe the growth of fibroblasts.The wound scratch assay was used to observe the migration of fibroblasts.Results:PRP group had more fibroblasts than FBS group and hydrogel group since Day 5 of culture,and exhibited greater fibroblast scratch migration area than FBS group on 48 h and 72 h of wound scratch assay(all p<.05).Conclusions:Compared with FBS,human fibroblasts cultured by PRP can more effectively promote the proliferation and migration of fibroblasts.
文摘Burn as a strong traumatic stimulus can induce inflammatory responses mediated by neuroendocrine,cytokines and other inflammatory mediators,and directly lead to organ dysfunction,resulting in severe MODS.Excessive inflammatory responses caused by necrotic tissues in the burned wound,also play an important role in stimulating the occurrence and the development of MODS.Complete removal of necrotic tissues in the early stage of burns to prevent from invasive wound infections,can effectively prevent MODS.AKI occurred earlier in this case,combined with its admission after the onset of shock and no obstruction of the urinary system can exclude prerenal and renal AKI,speculated as renal AKI.The factors leading to renal AKI are divided into renal ischemia and nephrotoxicity.The patient had adequate urinary output during shock and AKI in the presence of adequate renal blood flow,possibly due to the nephrotoxicity of a large number of necrotic tissues of the wound after they were broken down and absorbed into the bloodstream.The patient fell asleep after being drunk and the bedside stove broiled him for a long time,on one hand,heat accumulation lead to skin damage;on the other hand,sustained heat action caused hyperemia,edema and hemodynamic disorder in the deep tissues,and then resulted in secondary necrosis.
基金This work was supported by the National Key Research and Development Program of China(No.2018YFC1706001).
文摘Background:Aphasia is one of the common complications of stroke.Speech and language therapy is a conventional treatment for aphasia following stroke.However,rehabilitation often remains unsatisfactory after speech and language therapy alone.Acupuncture may be effective for aphasia after stroke.We designed this systematic review to assess the efficacy and safety of acupuncture as an adjunct to speech and language therapy for aphasia following ischemic stroke.Methods:An extensive search will be performed in databases including Cochrane Central Register of Controlled Trials,PubMed,Web of Science,EMBASE,China National Knowledge Infrastructure,Sinomed,Wei Pu Date and Wan Fang Data.No limitation will be set in language,publication date or status of the study.The primary outcomes include scores of Western Aphasia Battery and Aphasia Quotient.The secondary outcomes include Scales of Clinical Rehabilitation Research Center Aphasia Examination,Communicative Abilities in Daily Living,Aphasia Battery of Chinese,Chinese Functional Communication Profile,adverse reactions,etc.Results:All of the data will be processed with Review Manager software.Cochrane‘risk of bias’tool will be used to assess the risk of bias.Conclusion:This study will contribute to the selection of appropriate therapies for aphasia after ischemic stroke and the development of clinical guidelines on this topic.
文摘Inflammation is generally considered as a pathological process,and the occurrence of multi-organ inflammatory diseases is usually accompanied by an inflammatory response.Persistent inflammation can eventually lead to organ dysfunction.In recent years,the study of mesenchymal stem cell-derived exosomes has found its unique anti-inflammatory effect,which provides a new idea for the treatment of multi-organ inflammatory diseases.This article reviews the effect of mesenchymal stem cell exosomes on multi-organ inflammatory diseases.
文摘Objective: To explore the effects of combined application of culture supernatant of human umbilical cord mesenchymal stem cells (hUCMSCs) and ciprofloxacin on Staphylococcus aureus (SA) in vitro. Methods: hUCMSCs were isolated from umbilical cord tissues of full-term healthy fetuses after cesarean section and then cultured. Cells in the third passage were chosen for the use of experiment after identification. SA strains isolated from wounds of burn patients in our burn wards were used in the following experiment. Cells were divided into 0, 10, 100 and 1,000 ng/ml lipopolysaccharide (LPS) groups by use of the random number table (similarly hereinafter). Cells were cultured with culture medium containing mesenchymal stem cells (MSCs) after being treated with medium containing corresponding mass concen-trations of LPS for 12 h. At post culture hour (PCH) 6, 12 and 24, 6 wells of culture supernatant of cells in each group were obtained to measure the content of LL-37 with enzyme-linked immunosorbent assay (ELISA). Ninety blood agar culture plates were divided into ciprofloxacin control group (CC), ciprofloxacin + supernatant group (CS), and ciprofloxacin + supernatant+ LL-37 antibody group (CSL), with 30 blood agar culture plates in each group. Blood agar culture plates in group CC were coated with 1.5 × 108 colony forming unit (CFU)/ml bacteria solution prepared with normal saline. Blood agar culture plates in group CS were coated with 1.5 × 108 CFU/ml bacteria solution prepared with normal saline and hUCMSC culture supernatant (cultured by MSC culture medium, the same below) in double volume of normal saline. Blood agar culture plates in group CSL were coated with 1.5 × 108 CFU/ml bacteria solution prepared with normal saline, hUCMSC culture supernatant in double volume of normal saline, and 2.6 μL of LL-37 antibodies at the concentration of 2 μg/ml. At PCH 12, 24 and 48, 10 blood agar culture plates were taken out from each group to observe the distribution of SA colonies on blood agar culture plates and to measure diameters of zones of inhibition of ciprofloxacin. The minimum inhibitory concentration (MIC) of ciprofloxacin against SA in each group was recorded. Fractional inhibitory concentration (FIC) indexes of ciprofloxacin in group CS and group CSL at PCH 12, 24 and 48 were calculated, with the synergistic effect evaluated. Data were processed with factorial design ANOVA, one way ANOVA, LSD-t test, Kruskal-Wallis test and Mann-Whitney U test. Results: (1) At each PCH, the content of LL-37 in cell culture supernatant in 10 ng/ml LPS group, 100 ng/ml LPS group or 1,000 ng/ml LPS group was higher than that in 0 ng/ml LPS group (with t values ranging from 11.22 to 33.36, p values all below .01);the content of LL-37 in cell culture supernatant in either 100 ng/ml LPS group or 1,000 ng/ml LPS group was higher than that in 10 ng/ml LPS group (with t values ranging from 2.24 to 18.73, p < .05 or p < .01);the content of LL-37 in cell culture supernatant in 1,000 ng/ml LPS group was higher than that in 100 ng/ml LPS group (with t values ranging from 12.46 to 14.70, p values all below .01). (2) At PCH 12, 24 and 48, the bacterial colonies in groups CC, CS and CSL became integrated over time. In CC group, diameters of zones of inhibition of ciprofloxacin at PCH 12, 24 and 48 were 26 mm, 24 mm and 23 mm respectively, with no obvious changes. At PCH 12, 24 and 48, diameters of zones of inhibition of ciprofloxacin in groups CS and CSL were 82 mm, 71 mm, 68 mm and 74 mm, 59 mm, 56 mm respectively, which were significantly larger than those in group CC. (3) At each PCH, MIC of ciprofloxacin against SA in group CC was significantly higher than that in groups CS and CSL respectively (with Z values ranging from 6.22 to 6.71, p values all below .01);MIC of ciprofloxacin against SA in group CSL was significantly higher than that in group CS (with Z values all equal to 6.72, p values all below .01). (4) FIC indexes of ciprofloxacin in groups CS and CSL at PCH 12, 24 and 48 were 0.011, 0.032, 0.032 and 0.122, 0.350, 0.350, respectively. The results indicated that hUCMSC culture supernatant had a synergistically antibacterial effect when combined with ciprofloxacin. Conclusions: hUCMSCs can secrete LL-37, and the secretion level is improved with increase of LPS concentration. The com-bination of hUCMSC culture supernatant with ciprofloxacin can decrease the dosage of ciprofloxacin in resisting SA effectively. Once LL-37 is neutralized, the synergistically antibacterial effect of hUCMSC culture supernatant is decreased thereby.
基金This work was supported by funds from the National Natural Science Foundation of China(Nos.61871276 and 82071876)Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support(No.ZYLX202101)+1 种基金Research Foundation of Beijing Friendship Hospital(No.yyqdkt2019-30)Cultivation Scientific Research Foundation of Capital Medical University(No.1210020247).
文摘Background and Aims:Liver imaging reporting and data system(LI-RADS)provides standardized lexicon and categorization for diagnosing hepatocellular carcinoma(HCC).However,there is limited knowledge about the effect of LI-RADS training.We prospectively explored whether the systematic training of LI-RADS v2018 on magnetic resonance imaging(MRI)can effectively improve the diagnostic performances of different radiologists for HCC.Methods:A total of 20 visiting radiologists and the multiparametric MRI of 70 hepatic observations in 61 patients with high risk of HCC were included in this study.The LI-RADS v2018 training procedure included three times of thematic lectures(each lasting for 2.5 h)given by a professor specialized in imaging diagnosis of liver,with an interval of a month.After each seminar,the radiologists had a month to adopt the algorithm into their daily work.The diagnostic performances and interobserver agreements of these radiologists adopting the algorithm for HCC diagnosis before and after training were compared.Results:A total of 20 radiologists(male/female,12/8;with an average age of 36.75±4.99 years)were enrolled.After training,the interobserver agreements for the LI-RADS category for all radiologists(p=0.005)were increased.The sensitivity,specificity,positive predictive value,negative predictive value,and coincidence rate of all radiologists for HCC diagnosis before and after training were 43%vs.54%,86%vs.88%,74%vs.81%,62%vs.67%,and 65%vs.71%,respectively.The diagnostic performances of all radiologists(p<0.001)showed improvement after training.Conclusions:The systematic training of LI-RADS can effectively improve the diagnostic performances of radiologists with different experiences for HCC.
