Objective:To investigate antimicrobial effects of ethanolic extract of Zingiber zerumbet(Z.zerumbet)(L.)Smith and its chloroform and petroleum ether soluble fractions against pathogenic bacteria and fungi.Methods:The ...Objective:To investigate antimicrobial effects of ethanolic extract of Zingiber zerumbet(Z.zerumbet)(L.)Smith and its chloroform and petroleum ether soluble fractions against pathogenic bacteria and fungi.Methods:The fresh rhizomes of Zingiber zerumbet were extracted in cold with ethanol(4.0 L)after concentration.The crude ethanol extract was fractionated by petroleum ether and chloroform to form a suspension of ethanol extract(15.0 g),petroleum ether fraction(6.6 g)and chloroform soluble fraction(5.0 g).The crude ethanol extract and its petroleum ether and chloroform fractions were evaluated for antibacterial and antifungal activity against thirteen pathogenic bacteria and three fungi by the disc diffusion method.Commercially available kanamycin(30μg/disc)was used as standard disc and blank discs impregnated with the respective solvents were used as negative control.Results:At a concentration of 400μg/disc,all the samples showed mild to moderate antibacterial and antifungal activity and produced the zone of inhibition ranging from 6 mm to 10 mm.Among the tested samples,the crude ethanol extract showed the highest activity against Vibrio parahemolyticus(V.parahemolyticus).The minimum inhibitory concentration(MIC)of the crude ethanol extract and its fractions were within the value of 128-256μg/mL against two Gram positive and four Gram negative bacteria and all the samples showed the lowest MIC value against V.parahemolyticus(128μg/mL).Conclusions:It can be concluded that,potent antibacterial and antifungal phytochemicals are present in ethanol extract of Z.zerumbet(L.).展开更多
Forty different medicinal plants were investigated for arbuscular mycorrhizal association in the Rajshahi University Campus in Bangladesh. The results indicated that 35 different plants were infected by AM (arbuscular...Forty different medicinal plants were investigated for arbuscular mycorrhizal association in the Rajshahi University Campus in Bangladesh. The results indicated that 35 different plants were infected by AM (arbuscular mycorrhizal) fungi as found by trypan blue staining procedure. The percentage of root colonization by AM fungi varied from 13.3% to 100%. Mangifera indica and Morus indica have maximum percentage of colonization (100%). The intensity of root colonization were abundant in the plants belonging to the families Anacardiaceae, Asclepiadaceae, Moraceae, Leguminosae and Apocynaceae whereas the intensity of colonization of crop roots were moderate and poor belonging to Gramineae and Leguminosae. The presence of greater number of spore in soil was always associated with the incidence of abundant mycelia. In plant roots the formation of spore and mycelia was restricted by low pH. Number of mycorrhizal fungus spores ranged between 35 to100 per 100g air dried soil in different family respective soils. The frequency of mycorrhizal fungus infection showed positive correlation with soil pH, moisture, water holding capacity, texture, total nitrogen, organic carbon, phosphorus, calcium, potassium, and magnesium. Especially phosphorus and nitrogen in the soil greatly influenced the plant root infection by AM fungi.展开更多
Objective:To explore cytotoxic activity of ethanol extract of Alpinia calcarata Rose(EEAC) rhizome against Ehiiich ascites carcinoma(EAC) tumor bearing Swiss Albino mice.Methods: In the present study,its anti-neoplast...Objective:To explore cytotoxic activity of ethanol extract of Alpinia calcarata Rose(EEAC) rhizome against Ehiiich ascites carcinoma(EAC) tumor bearing Swiss Albino mice.Methods: In the present study,its anti-neoplastic activity has been studied by monitoring parameters like tumor weight measurement,survival time,tumor cell growth inhibition,haematological characteristics etc.Results:It was found that EEAC at dose 8 mg/kg/day(i.p.) significantly decreased tumor weight(62.0%;P【0.01),increased life span(70.25%;P 【0.01) and reduced tumor cell growth rate(85.7%;P【0.01) in comparison to those of EAC bearing mice.The plant extract also improved the depleted haematological parameters like RBC WBC,Hb%,differential counts(e.g.lymphocytes,neutrophils,monocytes etc) of EAC bearing mice towards normal.The host toxic effects were not very high and recovered gradually towards normal within a few days after treatment.ConchiskHis:EEAC exhibits potent in vivo cytotoxic activity against EAC tumor bearing Swiss Albino mice.So,the plant can be considered as a probable new source of antitumor agents.展开更多
Objective:To investigate the cytotoxicity of the crude ethanol extract of the rhizome of Zingiber zerumbel(Z.zerumbet)(L)Smith,and Curcuma zedoaria(C.zedoaria)Rosc,against Artemia salina Leach.Methods:Fresh rhizomes o...Objective:To investigate the cytotoxicity of the crude ethanol extract of the rhizome of Zingiber zerumbel(Z.zerumbet)(L)Smith,and Curcuma zedoaria(C.zedoaria)Rosc,against Artemia salina Leach.Methods:Fresh rhizomes of Z.zerumbet(L)Smith,and C.zedoaria Rose,were extracted separately in cold with ethanol(2.5 L)and after concentration a brownish syrupy suspension of ethanol extracts of Z.zerumbel(L)Smith,and C.zedoaria Rose,was obtained.The cytotoxic effect of the crude ethanol extracts of both plants was determined by brine shrimp lethality bioassay.Results:Crude ethanol extracts of the rhizome of Z.zerumbel(L)Smith,showed the highest cytotoxicity(LC_(50)was 1.24μg/mL)against brine shrimp nauplii as compared with C.zedoaria Rose.(LC_(50)was 33.593μg/mL)after 24 h of exposure.Conclusions:It can be concluded that the rhizome of Z.zerumbet(|L)Smith,and C.zedoaria Rose,can be used as a source of cytotoxic agent.展开更多
基金Supported by Innovation Technology,Research and Development Project,Ministry of Science.Information and Communication Technology.Republic of Bangladesh Government(No.12)
文摘Objective:To investigate antimicrobial effects of ethanolic extract of Zingiber zerumbet(Z.zerumbet)(L.)Smith and its chloroform and petroleum ether soluble fractions against pathogenic bacteria and fungi.Methods:The fresh rhizomes of Zingiber zerumbet were extracted in cold with ethanol(4.0 L)after concentration.The crude ethanol extract was fractionated by petroleum ether and chloroform to form a suspension of ethanol extract(15.0 g),petroleum ether fraction(6.6 g)and chloroform soluble fraction(5.0 g).The crude ethanol extract and its petroleum ether and chloroform fractions were evaluated for antibacterial and antifungal activity against thirteen pathogenic bacteria and three fungi by the disc diffusion method.Commercially available kanamycin(30μg/disc)was used as standard disc and blank discs impregnated with the respective solvents were used as negative control.Results:At a concentration of 400μg/disc,all the samples showed mild to moderate antibacterial and antifungal activity and produced the zone of inhibition ranging from 6 mm to 10 mm.Among the tested samples,the crude ethanol extract showed the highest activity against Vibrio parahemolyticus(V.parahemolyticus).The minimum inhibitory concentration(MIC)of the crude ethanol extract and its fractions were within the value of 128-256μg/mL against two Gram positive and four Gram negative bacteria and all the samples showed the lowest MIC value against V.parahemolyticus(128μg/mL).Conclusions:It can be concluded that,potent antibacterial and antifungal phytochemicals are present in ethanol extract of Z.zerumbet(L.).
文摘Forty different medicinal plants were investigated for arbuscular mycorrhizal association in the Rajshahi University Campus in Bangladesh. The results indicated that 35 different plants were infected by AM (arbuscular mycorrhizal) fungi as found by trypan blue staining procedure. The percentage of root colonization by AM fungi varied from 13.3% to 100%. Mangifera indica and Morus indica have maximum percentage of colonization (100%). The intensity of root colonization were abundant in the plants belonging to the families Anacardiaceae, Asclepiadaceae, Moraceae, Leguminosae and Apocynaceae whereas the intensity of colonization of crop roots were moderate and poor belonging to Gramineae and Leguminosae. The presence of greater number of spore in soil was always associated with the incidence of abundant mycelia. In plant roots the formation of spore and mycelia was restricted by low pH. Number of mycorrhizal fungus spores ranged between 35 to100 per 100g air dried soil in different family respective soils. The frequency of mycorrhizal fungus infection showed positive correlation with soil pH, moisture, water holding capacity, texture, total nitrogen, organic carbon, phosphorus, calcium, potassium, and magnesium. Especially phosphorus and nitrogen in the soil greatly influenced the plant root infection by AM fungi.
文摘Objective:To explore cytotoxic activity of ethanol extract of Alpinia calcarata Rose(EEAC) rhizome against Ehiiich ascites carcinoma(EAC) tumor bearing Swiss Albino mice.Methods: In the present study,its anti-neoplastic activity has been studied by monitoring parameters like tumor weight measurement,survival time,tumor cell growth inhibition,haematological characteristics etc.Results:It was found that EEAC at dose 8 mg/kg/day(i.p.) significantly decreased tumor weight(62.0%;P【0.01),increased life span(70.25%;P 【0.01) and reduced tumor cell growth rate(85.7%;P【0.01) in comparison to those of EAC bearing mice.The plant extract also improved the depleted haematological parameters like RBC WBC,Hb%,differential counts(e.g.lymphocytes,neutrophils,monocytes etc) of EAC bearing mice towards normal.The host toxic effects were not very high and recovered gradually towards normal within a few days after treatment.ConchiskHis:EEAC exhibits potent in vivo cytotoxic activity against EAC tumor bearing Swiss Albino mice.So,the plant can be considered as a probable new source of antitumor agents.
基金supported by Ministry of Science,Information and Communication Technology,Bangladesh(grant No.12/2008-2009)
文摘Objective:To investigate the cytotoxicity of the crude ethanol extract of the rhizome of Zingiber zerumbel(Z.zerumbet)(L)Smith,and Curcuma zedoaria(C.zedoaria)Rosc,against Artemia salina Leach.Methods:Fresh rhizomes of Z.zerumbet(L)Smith,and C.zedoaria Rose,were extracted separately in cold with ethanol(2.5 L)and after concentration a brownish syrupy suspension of ethanol extracts of Z.zerumbel(L)Smith,and C.zedoaria Rose,was obtained.The cytotoxic effect of the crude ethanol extracts of both plants was determined by brine shrimp lethality bioassay.Results:Crude ethanol extracts of the rhizome of Z.zerumbel(L)Smith,showed the highest cytotoxicity(LC_(50)was 1.24μg/mL)against brine shrimp nauplii as compared with C.zedoaria Rose.(LC_(50)was 33.593μg/mL)after 24 h of exposure.Conclusions:It can be concluded that the rhizome of Z.zerumbet(|L)Smith,and C.zedoaria Rose,can be used as a source of cytotoxic agent.
