Photodynamic diagnosis based on 5-aminolevulinic acidinduced protoporphyrin IX has been clinically applied in many fields based upon its evidenced efficacy and adequate safety. In order to establish a personalized med...Photodynamic diagnosis based on 5-aminolevulinic acidinduced protoporphyrin IX has been clinically applied in many fields based upon its evidenced efficacy and adequate safety. In order to establish a personalized medicine approach for treating gastric cancer patients, rapid intraoperative detection of malignant lesions has become important. Feasibility of photodynamic diagnosis using 5-aminolevulinic acid for gastric cancer patients has been investigated, especially for the detection of peritoneal dissemination and lymph node metastasis. This method enables intraoperative realtime fluorescence detection of peritoneal dissemination, exhibiting higher sensitivity than white light observation without histopathological examination. The method also enables detection of metastatic foci within excised lymph nodes, exhibiting a diagnostic accuracy comparable to that of a current molecular diagnostics technique. Although several complicating issues still need to be resolved, such as the effect of tissue autofluorescence and the insufficient depth penetration of excitation light, this simple and rapid method has the potential to become a useful diagnostic tool for gastric cancer, as well as urinary bladder cancer and glioma.展开更多
We demonstrate long-range enhancement of fluorescence and Raman scattering using a dense random array of Ag nanoislands(AgNIs)coated with column-structured silica(CSS)overlayer of over 100 nm thickness,namely,remote p...We demonstrate long-range enhancement of fluorescence and Raman scattering using a dense random array of Ag nanoislands(AgNIs)coated with column-structured silica(CSS)overlayer of over 100 nm thickness,namely,remote plasmonic-like enhancement(RPE).The CSS layer provides physical and chemical protection,reducing the impact between analyte molecules and metal nanostructures.RPE plates are fabricated with high productivity using sputtering and chemical immersion in gold(I)/halide solution.The RPE plate significantly enhances Raman scattering and fluorescence,even without proximity between analyte molecules and metal nanostructures.The maximum enhancement factors are 10^(7)-fold for Raman scattering and 102-fold for fluorescence.RPE is successfully applied to enhance fluorescence biosensing of intracellular signalling dynamics in HeLa cells and Raman histological imaging of oesophagus tissues.Our findings present an interesting deviation from the conventional near-field enhancement theory,as they cannot be readily explained within its framework.However,based on the phenomenological aspects we have demonstrated,the observed enhancement is likely associated with the remote resonant coupling between the localised surface plasmon of AgNIs and the molecular transition dipole of the analyte,facilitated through the CSS structure.Although further investigation is warranted to fully understand the underlying mechanisms,the RPE plate offers practical advantages,such as high productivity and biocompatibility,making it a valuable tool for biosensing and biomolecular analysis in chemistry,biology,and medicine.We anticipate that RPE will advance as a versatile analytical tool for enhanced biosensing using Raman and fluorescence analysis in various biological contexts.展开更多
文摘Photodynamic diagnosis based on 5-aminolevulinic acidinduced protoporphyrin IX has been clinically applied in many fields based upon its evidenced efficacy and adequate safety. In order to establish a personalized medicine approach for treating gastric cancer patients, rapid intraoperative detection of malignant lesions has become important. Feasibility of photodynamic diagnosis using 5-aminolevulinic acid for gastric cancer patients has been investigated, especially for the detection of peritoneal dissemination and lymph node metastasis. This method enables intraoperative realtime fluorescence detection of peritoneal dissemination, exhibiting higher sensitivity than white light observation without histopathological examination. The method also enables detection of metastatic foci within excised lymph nodes, exhibiting a diagnostic accuracy comparable to that of a current molecular diagnostics technique. Although several complicating issues still need to be resolved, such as the effect of tissue autofluorescence and the insufficient depth penetration of excitation light, this simple and rapid method has the potential to become a useful diagnostic tool for gastric cancer, as well as urinary bladder cancer and glioma.
基金supported by the PRESTO program(JPMJPR17PC)of Japan Science and Technology Agency(JST),Japan,JSPS KAKENHI(15K12519,19K22969,21H01847,22H00303)from the Japan Society for the Promotion of Science(JSPS),Japan,a research grant from the Research Clusters program of Tokushima University(1802003)support from Mr K.Hayashi(Kyoto University),Mr A.Aiba,Mr T.Saito,Ms H.Miura(Ushio Inc.),and Mr S.Sugano(Tokushima University)。
文摘We demonstrate long-range enhancement of fluorescence and Raman scattering using a dense random array of Ag nanoislands(AgNIs)coated with column-structured silica(CSS)overlayer of over 100 nm thickness,namely,remote plasmonic-like enhancement(RPE).The CSS layer provides physical and chemical protection,reducing the impact between analyte molecules and metal nanostructures.RPE plates are fabricated with high productivity using sputtering and chemical immersion in gold(I)/halide solution.The RPE plate significantly enhances Raman scattering and fluorescence,even without proximity between analyte molecules and metal nanostructures.The maximum enhancement factors are 10^(7)-fold for Raman scattering and 102-fold for fluorescence.RPE is successfully applied to enhance fluorescence biosensing of intracellular signalling dynamics in HeLa cells and Raman histological imaging of oesophagus tissues.Our findings present an interesting deviation from the conventional near-field enhancement theory,as they cannot be readily explained within its framework.However,based on the phenomenological aspects we have demonstrated,the observed enhancement is likely associated with the remote resonant coupling between the localised surface plasmon of AgNIs and the molecular transition dipole of the analyte,facilitated through the CSS structure.Although further investigation is warranted to fully understand the underlying mechanisms,the RPE plate offers practical advantages,such as high productivity and biocompatibility,making it a valuable tool for biosensing and biomolecular analysis in chemistry,biology,and medicine.We anticipate that RPE will advance as a versatile analytical tool for enhanced biosensing using Raman and fluorescence analysis in various biological contexts.
