OBJECTIVE： To investigate the role of spinal prostaglandin E2 （PGE2） in electroacupuncture （EA） analgesia and assess the theoretical basis for selection of acupoints in the treatment of neuropathic pain. METHODS...OBJECTIVE： To investigate the role of spinal prostaglandin E2 （PGE2） in electroacupuncture （EA） analgesia and assess the theoretical basis for selection of acupoints in the treatment of neuropathic pain. METHODS： A rat model of neuropathic pain was established. Rats were randomly divided into normal, model, sham, EA 1, EA 2, and EA 3 groups. In EA 1 group, the rats were needled at bilateral L5 Jiaji （EX-B2）, Dachangshu （BL 25）, Weizhong （BL 40） and Kunlun （BL 60）. In EA 2 group, the rats were needled at bilateral Weizhong （BL 40） and Kunlun （BL 60）. In EA 3 group, the rats were needled at bilateral L5 Jiaji （EX-B2） and Dachangshu （BL 25）. EA stimula- tion was administered once daily over 7 days. Motor function and thermal withdrawal latencies were evaluated at 1 day preoperatively and at 3, 5, and 7 days postoperatively. After 7 days of intervention,enzyme-linKed immunosorDnen~ assay （ELISA） Wd3 used to quantify the expression of the spinal PGE2. RESULTS： Rats in the model group exhibited evident hyperalgesia in responses to thermal with- drawal latencies compared with those in the control group （P 〈 0.01）, and EA reversed thermal withdrawal latencies （P 〈 0.01）. The expression level of the spinal PGE2 was significantly higher in the model group than that in the control group and was reversed by EA （P 〈 0.01; P 〈 0.05）. CONCLUSION： The effect of EA on neuropathic pain might alleviate the hyperalgesia state by an inhibition of local prostaglandin E2 secretion.展开更多
OBJECTIVE: To observe the effect of electroacupuncture(EA) treatment on the expression of E-prostanoid 2(EP2) in the spinal cord using a rat model of neuropathic pain, and to examine the effect of different acupunctur...OBJECTIVE: To observe the effect of electroacupuncture(EA) treatment on the expression of E-prostanoid 2(EP2) in the spinal cord using a rat model of neuropathic pain, and to examine the effect of different acupuncture prescriptions.METHODS: Rats were randomly divided into six groups: a normal group, model group, sham group,EA 1 group(distal-proximal acupoints), EA 2 group(proximal acupoints), and EA 3 group(distal acupoints). Rats in the EA groups were treated for20 min, once daily for 7 days. The mechanical withdrawal threshold was evaluated at 1 day preoperatively and at 3, 5, and 7 days postoperatively. At 7days postoperatively, hematoxylin-eosin(HE) staining was used to investigate spinal morphological changes. Spinal EP2 messenger ribonucleic acid(m RNA) was detected using reverse-transcription polymerase chain reaction.RESULTS: After the EA treatment, the mechanical withdrawal threshold was up-regulated compared with the model group(P < 0.05). The expression of EP2 m RNA was up-regulated in the spinal cord on day 7 after surgery(P < 0.05). EA stimulation tended to down-regulate EP2 m RNA expression compared with the model group(P > 0.05). The EA 1and EA 2 groups showed a larger treatment effect than the EA 3 group. The HE results showed that EA can improve spinal histomorphology induced by neuropathic pain.CONCLUSION: EA had a strong analgesic and anti-inflammatory effect. The therapeutic effect of EA on the down-regulation of spinal EP2 m RNA expression may be greater when either local acupoints or a distal-proximal combination of acupoints are used than when distal points are used.展开更多
目的:基于CiteSpace知识图谱可视化分析探讨针刺治疗肛肠病术后疼痛领域的研究热点和趋势,以期为本病临床防治和研究提供依据。方法:以中国知网、万方数据、维普网及Web of Science(科学网,WOS)收录的数据库建库至2022年12月31日期间针...目的:基于CiteSpace知识图谱可视化分析探讨针刺治疗肛肠病术后疼痛领域的研究热点和趋势,以期为本病临床防治和研究提供依据。方法:以中国知网、万方数据、维普网及Web of Science(科学网,WOS)收录的数据库建库至2022年12月31日期间针刺治疗肛肠病术后疼痛研究相关文献为研究对象,采用CiteSpace进行文献计量学分析。结果:共纳入符合标准的中文文献326篇、英文文献4篇;中文发文量最多的作者为何剑平、张锡滔和张敏3位作者,WOS中共发文4篇,其作者分别为Wu JY、Zhao Y、Qin D和Yeh ML;中文发文最多的研究机构为中国中医科学院广安门医院;WOS发文量最多的国家为中国,中心性最高的为中国;中文文献高频关键词有“疼痛”“术后疼痛”“混合痔”“穴位贴敷”“穴位埋线”等;WOS高频关键词有“Hemorrhoid(痔疮)”“Electroacupuncture(电针)”“Complementary therapy(补充疗法)”“Analgesia(镇痛)”“Acupuncture(针刺)”等;中文关键词形成10个聚类;中文文献得到14个突显词,主要是“痔瘘术后”“手术后”“肛肠病”“穴位按压”“白环俞”等。结论:在针刺治疗肛肠病术后疼痛的临床研究方面,研究热点起初以缓解疼痛为主,随后探寻手术方式以减少肛肠病术后疼痛,近年来针刺治疗肛肠病术后疼痛走向主流,同时本领域研究出现了更多高质量的对照试验以及临床试验,但仍缺少相关机制的探索及基础研究,亦是未来的研究热点之一。展开更多
目的对医院脑卒中的急救医疗服务（emergency medical service，EMS）流程进行调查，以提高脑卒中EMS的质量，为完善脑卒中EMS提供依据。方法2013年6月一2015年7月由石河子大学医学院第一附属医院急救中心调查研究人员亲自参与EMS，并...目的对医院脑卒中的急救医疗服务（emergency medical service，EMS）流程进行调查，以提高脑卒中EMS的质量，为完善脑卒中EMS提供依据。方法2013年6月一2015年7月由石河子大学医学院第一附属医院急救中心调查研究人员亲自参与EMS，并且将医院的EMS细分成4个阶段：EMS启动、反应、现场救治、转运。使用脑血管病防治指南和美国缺血性脑卒中自制调查表和早期处理指南进行调查，研究对象包括脑卒中患者和家属，对EMS救治人员的反应能力、反应速度和现场处理措施进行评价。结果脑卒中EMS中120急救电话分别是由患者、旁观者和家属拨打。从患者开始发病到拨打电话的过程，时间在3min以内的占7．33％，在30min以内的占34．67％，超30min的占58％。在拨打急救电话后，有84％的急救车能够及时出发并到达。EMS人员未使用院外卒中工具进行筛选，对60．12％的患者未进行相关的处理和鉴别。结论在一般情况下，同相关的指南作比较，EMS人员询问大多数患者的既往史，为患者通知相关医院，并且进行相关治疗，体现了急救医疗的重要性。展开更多
基金Supported by the Grants from the Project of Beijing University of Chinese Medicine:Studies on Effect of Electroacupuncture at Different Acupoint Modules in the Rat Model of Prolapse of Lumbar Intervertebral disc by COX2-PGE2 Pathway and Mechanisms of Acupoints Compatibility(No.JYB22-JS022)
文摘OBJECTIVE： To investigate the role of spinal prostaglandin E2 （PGE2） in electroacupuncture （EA） analgesia and assess the theoretical basis for selection of acupoints in the treatment of neuropathic pain. METHODS： A rat model of neuropathic pain was established. Rats were randomly divided into normal, model, sham, EA 1, EA 2, and EA 3 groups. In EA 1 group, the rats were needled at bilateral L5 Jiaji （EX-B2）, Dachangshu （BL 25）, Weizhong （BL 40） and Kunlun （BL 60）. In EA 2 group, the rats were needled at bilateral Weizhong （BL 40） and Kunlun （BL 60）. In EA 3 group, the rats were needled at bilateral L5 Jiaji （EX-B2） and Dachangshu （BL 25）. EA stimula- tion was administered once daily over 7 days. Motor function and thermal withdrawal latencies were evaluated at 1 day preoperatively and at 3, 5, and 7 days postoperatively. After 7 days of intervention,enzyme-linKed immunosorDnen~ assay （ELISA） Wd3 used to quantify the expression of the spinal PGE2. RESULTS： Rats in the model group exhibited evident hyperalgesia in responses to thermal with- drawal latencies compared with those in the control group （P 〈 0.01）, and EA reversed thermal withdrawal latencies （P 〈 0.01）. The expression level of the spinal PGE2 was significantly higher in the model group than that in the control group and was reversed by EA （P 〈 0.01; P 〈 0.05）. CONCLUSION： The effect of EA on neuropathic pain might alleviate the hyperalgesia state by an inhibition of local prostaglandin E2 secretion.
基金Supported by Grants from the Project of Beijing University of Chinese Medicine in China:Studies on Effect of Electroacupuncture at Different Acupoint Modules in the Rat Model Lumbar Disc Herniation By COX2-PGE2 Pathway and Mechanisms of Acupoints Compatibility(No.JYB22-JS022)
文摘OBJECTIVE: To observe the effect of electroacupuncture(EA) treatment on the expression of E-prostanoid 2(EP2) in the spinal cord using a rat model of neuropathic pain, and to examine the effect of different acupuncture prescriptions.METHODS: Rats were randomly divided into six groups: a normal group, model group, sham group,EA 1 group(distal-proximal acupoints), EA 2 group(proximal acupoints), and EA 3 group(distal acupoints). Rats in the EA groups were treated for20 min, once daily for 7 days. The mechanical withdrawal threshold was evaluated at 1 day preoperatively and at 3, 5, and 7 days postoperatively. At 7days postoperatively, hematoxylin-eosin(HE) staining was used to investigate spinal morphological changes. Spinal EP2 messenger ribonucleic acid(m RNA) was detected using reverse-transcription polymerase chain reaction.RESULTS: After the EA treatment, the mechanical withdrawal threshold was up-regulated compared with the model group(P < 0.05). The expression of EP2 m RNA was up-regulated in the spinal cord on day 7 after surgery(P < 0.05). EA stimulation tended to down-regulate EP2 m RNA expression compared with the model group(P > 0.05). The EA 1and EA 2 groups showed a larger treatment effect than the EA 3 group. The HE results showed that EA can improve spinal histomorphology induced by neuropathic pain.CONCLUSION: EA had a strong analgesic and anti-inflammatory effect. The therapeutic effect of EA on the down-regulation of spinal EP2 m RNA expression may be greater when either local acupoints or a distal-proximal combination of acupoints are used than when distal points are used.
文摘目的对医院脑卒中的急救医疗服务（emergency medical service，EMS）流程进行调查，以提高脑卒中EMS的质量，为完善脑卒中EMS提供依据。方法2013年6月一2015年7月由石河子大学医学院第一附属医院急救中心调查研究人员亲自参与EMS，并且将医院的EMS细分成4个阶段：EMS启动、反应、现场救治、转运。使用脑血管病防治指南和美国缺血性脑卒中自制调查表和早期处理指南进行调查，研究对象包括脑卒中患者和家属，对EMS救治人员的反应能力、反应速度和现场处理措施进行评价。结果脑卒中EMS中120急救电话分别是由患者、旁观者和家属拨打。从患者开始发病到拨打电话的过程，时间在3min以内的占7．33％，在30min以内的占34．67％，超30min的占58％。在拨打急救电话后，有84％的急救车能够及时出发并到达。EMS人员未使用院外卒中工具进行筛选，对60．12％的患者未进行相关的处理和鉴别。结论在一般情况下，同相关的指南作比较，EMS人员询问大多数患者的既往史，为患者通知相关医院，并且进行相关治疗，体现了急救医疗的重要性。
