The dark blue pigment(DBP)is a health ingredient from Vaccinium bracteatum Thunb.leaves used as a functional food supplement.However,the details of transepithelial absorption on the intestinal epithelial cells are bar...The dark blue pigment(DBP)is a health ingredient from Vaccinium bracteatum Thunb.leaves used as a functional food supplement.However,the details of transepithelial absorption on the intestinal epithelial cells are barely understood.This study aimed to clarify the absorption properties of DBP in the Caco-2 cell monolayer model and evaluate the effect on the endo-metabolism and barrier function of Caco-2 cells.The results showed that the DBP did not show the dose-dependent toxic effect to Caco-2 cells between 0.25 and 1.5 mg/mL,which did not cause disorder in the normal cell metabolism and absorption activity.The Caco-2 cell monolayer model could absorb DBP by passive and active transport,and the absorptive pattern was dosedependent when the concentration was more than 0.25 mg/mL.During DBP absorption,an increase in m RNA and protein expressions of glucose transporters demonstrated that the glucose transporters were the potential transporter of DBP.But the glucose transport amounts were significantly lowered after 30 min of DBP treatment,indicating that DBP owned the inhibitory effect on glucose transportation.Furthermore,DBP also owned protective effects on the barrier function of intestinal epithelial cells.展开更多
We report a systematic study on the transport properties of(Bi_(0.2)Sb_(0.8))_(2)Te_(3)and(Bi_(0.4)Sb_(0.6))_(2)Te_(3)nanoplates with a thickness of about 6 nm grown by chemical vapor deposition(CVD)on Si/SiO_(2)subst...We report a systematic study on the transport properties of(Bi_(0.2)Sb_(0.8))_(2)Te_(3)and(Bi_(0.4)Sb_(0.6))_(2)Te_(3)nanoplates with a thickness of about 6 nm grown by chemical vapor deposition(CVD)on Si/SiO_(2)substrate.We achieve a significant ambipolar field effect in the two samples with different compositions by applying back-gate voltage,successfully tuning the Fermi level across the Dirac point of surface states.It is found that the Hall resistance exhibits strong non-linear behavior and magnetic field induced sign change of the slope when the Fermi level is near the Dirac point,indicating the coexistence of n-type and p-type carriers.Moreover,this coincides with the striking crossover from weak antilocalization(WAL)to linear magnetoresistance(LMR).These gate and temperature dependent magneto-transport studies provide a deeper insight into the nature of LMR and WAL in topological materials.展开更多
Structure and composition of Earth are fundamental importance in exploring the dynamic evolution of the crust and mantle.The Qinling Orogenic Belt(QOB)is located between the North China plate and the South China Plate...Structure and composition of Earth are fundamental importance in exploring the dynamic evolution of the crust and mantle.The Qinling Orogenic Belt(QOB)is located between the North China plate and the South China Plate,and is one of the main orogenic belts in China.To explore the composition and origin of anisotropy and the low wave velocity zone of the QOB,ten rock samples(gneiss and schist)were collected from the five sites of the QOB and the P-and S-wave velocities of these samples were measured under 0.6 to 2.0 GPa and 100 to 550℃.The wave velocities increase with increasing pressure and decreasing temperature.The V_(P)and V_(S)of the schist and gneiss match the velocity of the middle and lower crust of the QOB,indicating that schist and gneiss are important component of the QOB.All the schist and gneiss samples exhibit obvious seismic anisotropy with 1.64%-17.42%for V_(S)and 2.93%-14.78%for V_(P)under conditions of crust and upper mantle.The CPO/LPO and layering distribution of mica in rock samples are the main reasons for this anisotropy.The V_(S)structures below the five sampled sites from seismic ambient noise tomography were built to explore the effect of schist and gneiss on the composition and structure of the QOB.The results indicate that orientation-arranged gneiss and schist driven by the tectonic stresses might be a new origin of the character of V_(P)/V_(S),seismic anisotropy,and the low velocity zone in the QOB.展开更多
With the continuous advancement of big data technology,its application in precision agriculture is becoming more and more extensive,which provides a new path for agricultural modernization.Based on the background of b...With the continuous advancement of big data technology,its application in precision agriculture is becoming more and more extensive,which provides a new path for agricultural modernization.Based on the background of big data technology,this paper discusses the development path of precision agriculture.The research shows that the application of big data technology in precision agriculture is mainly reflected in crop growth monitoring and prediction,soil fertility assessment and regulation,water resources management and optimization,agricultural product traceability and origin tracing,and agricultural product supply chain management and risk control.However,the application of big data technology in the agricultural field stll encounters numerous challenges,such as data collection and processing,data analysis and application,and privacy protection and security.To this end,this paper puts forward countermeasures,including strengthening infrastructure construction and establishing data sharing and management mechanisms.展开更多
“I call on all leaders worldwide to declare a State of Climate Emergency in their own countries until carbon neutrality is reached.”–António GUTERRES(United Nations Secretary General),12 December,2020 There is...“I call on all leaders worldwide to declare a State of Climate Emergency in their own countries until carbon neutrality is reached.”–António GUTERRES(United Nations Secretary General),12 December,2020 There is no shortcut to a carbon neutral society;solutions are urgently required from both energy&industrial sectors and global ecosystems.While the former is often held accountable and emphasized in terms of its emissions reduction capability,the latter(recently termed natural climate solutions)should also be assessed for potential and limitations by the scientific community,the public,and policy makers.展开更多
H9N2 subtype avian influenza virus(AIV)is an influenza A virus that is widely spread throughout Asia,where it jeopardizes the poultry industry and provides genetic material for emerging human pathogens.To better under...H9N2 subtype avian influenza virus(AIV)is an influenza A virus that is widely spread throughout Asia,where it jeopardizes the poultry industry and provides genetic material for emerging human pathogens.To better understand the epidemicity and genetics of H9 subtype AIVs,we conducted active surveillance in live poultry markets(LPMs)in Hubei Province from 2013 to 2017.A total of 4798 samples were collected from apparent healthy poultry and environment.Realtime RT-PCR revealed that the positivity rate of influenza A was 26.6%(1275/4798),of which the H9 subtype accounted for 50.3%(641/1275)of the positive samples.Of the 132 H9N2 viral strains isolated,48 representative strains were subjected to evolutionary analysis and genotyping.Phylogenetic analysis revealed that all H9N2 viral genes had 91.1%–100%nucleotide homology,clustered with genotype 57,and had high homology with human H9N2 viruses isolated from2013 to 2017 in China.Using a nucleotide divergence cutoff of 95%,we identified ten distinct H9N2 genotypes that continued to change over time.Molecular analysis demonstrated that six H9N2 isolates had additional potential glycosylation sites at position 218 in the hemagglutinin protein,and all isolates had I155 T and Q226 L mutations.Moreover,44 strains had A558 V mutations in the PB2 protein and four had E627 V mutations,along with H9N2 human infection strains A/Beijing/1/2016 and A/Beijing/1/2017.These results emphasize that the H9N2 influenza virus in Hubei continues to mutate and undergo mammalian adaptation changes,indicating the necessity of strengthening the surveillance of the AIV H9N2 subtype in LPMs.展开更多
Mammalian bone is constantly metabolized from the embryonic stage,and the maintenance of bone health depends on the dynamic balance between bone resorption and bone formation,mediated by osteoclasts and osteoblasts.It...Mammalian bone is constantly metabolized from the embryonic stage,and the maintenance of bone health depends on the dynamic balance between bone resorption and bone formation,mediated by osteoclasts and osteoblasts.It is widely recognized that circadian clock genes can regulate bone metabolism.In recent years,the regulation of bone metabolism by non-coding RNAs has become a hotspot of research.MicroRNAs can participate in bone catabolism and anabolism by targeting key factors related to bone metabolism,including circadian clock genes.However,research in this field has been conducted only in recent years and the mechanisms involved are not yet well established.Recent studies have focused on how to target circadian clock genes to treat some diseases,such as autoimmune diseases,but few have focused on the co-regulation of circadian clock genes and microRNAs in bone metabolic diseases.Therefore,in this paper we review the progress of research on the co-regulation of bone metabolism by circadian clock genes and microRNAs,aiming to provide new ideas for the prevention and treatment of bone metabolic diseases such as osteoporosis.展开更多
Little is known about the prevalence of drug-resistant mutations in HIV-1-positive individuals in Suzhou, China. To elucidate the transmitted drug resistance(TDR) and acquired drug resistance mutation(ADR) profiles, w...Little is known about the prevalence of drug-resistant mutations in HIV-1-positive individuals in Suzhou, China. To elucidate the transmitted drug resistance(TDR) and acquired drug resistance mutation(ADR) profiles, we collected blood specimens from 127 drug-naive and 117 first-line drugtreated HIV-1-infected individuals sampled from 2014 to 2016 in Suzhou. We successfully amplified po/fragments from 100 drug-naive and 20 drug-treated samples. We then determined the drugresistant mutations to protease(PR) and reverse-transcriptase(RT) inhibitors according to the Stanford drug resistance database. Overall, 11 and 13 individuals had transmitted(drug-naive group) and acquired(treated group) resistance mutations, respectively. Six transmitted drugresistant mutations were found, including two mutations(L33F and L76V) in the protease region and four(K70N/E and V179D/E) in the RT region. Only L76 V was a major mutation, and K70N/E and V179D/E are known to cause low-level resistance to RT inhibitors. All 13 treated participants who had major drug resistance mutations demonstrated intermediate to high resistance to efavirenz and nevirapine, and six had a treatment duration of less than three months. No major mutations to RT inhibitors were found, implying that the epidemic of transmitted resistance mutations was not significant in this area. Our results suggest that more frequent virus load and drug resistance mutation tests should be conducted for individuals receiving antiretroviral treatment, especially for newly treated patients. Our research provides insights into the occurrence of HIV-1 drug resistance in Suzhou and will help to optimize the treatment strategy for this population.展开更多
Titanium oxide(TiO_(2)),with excellent cycling stability and low volume expansion,is a promising anode material for lithium-ion battery(LIB),which suffers from low electrical conductivity and poor rate capability.Comb...Titanium oxide(TiO_(2)),with excellent cycling stability and low volume expansion,is a promising anode material for lithium-ion battery(LIB),which suffers from low electrical conductivity and poor rate capability.Combining nano-sized TiO_(2)with conductive materials is proved an efficient method to improve its electrochemical properties.Here,rutile TiO_(2)/carbon nanosheet was obtained by calcinating MAX(Ti_(3)AlC_(2))and Na_(2)C0_(3)together and water-bathing with HC1.The lamellar carbon atoms in MAX are converted to 2D carbon nanosheets with urchin-like rutile TiO_(2)anchored on.The unique architecture can offer plentiful active sites,shorten the ion diffusion distance and improve the conductivity.The composite exhibits a high reversible capacity of 247 mA h g^-1,excellent rate performance(38 mA h g^-1 at 50 C)and stable cycling performance(0.014%decay per cycle during 2000 cycles)for lithium storage.展开更多
Mutations in long-chain acyl-CoA synthetase 4 (ACSL4) are associated with non-syndromic X-linked intellectual disability (ID). However, the neural functions of ACSL4 and how loss of ACSL4 leads to ID remain largely un...Mutations in long-chain acyl-CoA synthetase 4 (ACSL4) are associated with non-syndromic X-linked intellectual disability (ID). However, the neural functions of ACSL4 and how loss of ACSL4 leads to ID remain largely unexplored. We report here that mutations in Acsl, the Drosophila ortholog of human ACSL3 and ACSL4, result in developmental defects of the mushroom body (MB), the center of olfactory learning and memory. Specifically, Acsl mutants show fewer MB neuroblasts (Nbs) due to reduced proliferation activity and premature differentiation. Consistently, these surviving Nbs show reduced expression of cyclin E, a key regulator of the G1-to S-phase cell cycle transition, and nuclear mislocalization of the transcriptional factor Prospero, which is known to repress self-renewal genes and activate differentiating genes. Furthermore, RNA-seq analysis reveals downregulated Nb-and cell-cyclerelated genes and upregulated neuronal differentiation genes in Acsl mutant Nbs. As Drosophila Acsl and human ACSL4 are functionally conserved, our findings provide novel insights into a critical and previously unappreciated role of Acsl in neurogenesis and the pathogenesis of ACSL4-related ID.展开更多
The potential geographic distribution and favorable climatic conditions of Machilus thunbergii under current and future predicted climates in China are predicted based on MaxEnt model and ArcGIS software. The results ...The potential geographic distribution and favorable climatic conditions of Machilus thunbergii under current and future predicted climates in China are predicted based on MaxEnt model and ArcGIS software. The results show that the AUC values in different time periods and emission seniors are more than 0.9, which indicates the prediction is excellent. Precipitation of the coldest quarter, precipitation of the driest month, annual precipitation, mean diurnal range, and temperature annual range are the most important environmental factors affecting the distribution of Machilus thunbergii. At present, the suitable areas of Machilus thunbergii are mainly concentrated in the eastern subtropics of China, with a total area of 118.47 × 104 km2. The medium-suitability area and the high-suitability area are concentrated in Wuyi Mountains, Luoxiao Mountains, Xuefeng Mountains, Nanling and east of Taiwan Mountains. With the change of climate, the suitable area increases, and the medium-suitability area and high-suitability area migrate and expand to the east, and the low-suitability area expands slightly to the west and north. So, the simulated distribution of Machilus thunbergii should be one of priorities, when instigating in-situ conservation. The research results can provide a theoretical reference for the popularization and planting of Machilus thunbergii.展开更多
Excessive abdominal fat deposition seriously restricts the production efficiency of broilers.Several studies found that dietary supplemental manganese(Mn)could effectively reduce the abdominal fat deposition of broile...Excessive abdominal fat deposition seriously restricts the production efficiency of broilers.Several studies found that dietary supplemental manganese(Mn)could effectively reduce the abdominal fat deposition of broilers,but the underlying mechanisms remain unclear.The present study aimed to investigate the effect of dietary supplementation with the inorganic or organic Mn on abdominal fat deposition,and enzyme activity and gene expression involved in lipid metabolism in the abdominal fat of male or female broilers.A total of 4201-d-old AA broilers(half males and half females)were randomly allotted by body weight and gender to 1 of 6 treatments with 10 replicates cages of 7 chicks per cage in a completely randomized design involving a 3(dietary Mn addition)×2(gender)factorial arrangement.Male or female broilers were fed with the Mn-unsupplemented basal diets containing 17.52 mg Mn kg^(-1)(d 1-21)and 15.62 mg Mn kg^(-1)(d 22-42)by analysis or the basal diets supplemented with 110 mg Mn kg^(-1)(d 1-21)and 80 mg Mn kg^(-1)(d 22-42)as either the Mn sulfate or the Mn proteinate with moderate chelation strength(Mn-Prot M)for 42 d.The results showed that the interaction between dietary Mn addition and gender had no impact(P>0.05)on any of the measured parameters;abdominal fat percentage of broilers was decreased(P<0.003)by Mn addition;Mn addition increased(P<0.004)adipose triglyceride lipase(ATGL)activity,while Mn-Prot M decreased(P<0.002)the fatty acid synthase(FAS)activity in the abdominal fat of broilers compared to the control;Mn addition decreased(P<0.009)diacylglycerol acyltransferase 2(DGAT2)mRNA expression level and peroxisome proliferator-activated receptor γ(PPARγ)mRNA and protein expression levels,but up-regulated(P<0.05)the ATGL mRNA and protein expression levels in the abdominal fat of broilers.It was concluded that dietary supplementation with Mn inhibited the abdominal fat deposition of broilers possibly via decreasing the expression of PPARγand DGAT2 as well as increasing the expression and activity of ATGL in the abdominal fat of broilers,and Mn-Prot M was more effective in inhibiting the FAS acitivity.展开更多
Strawberry(Fragaria spp.)is a member of the Rosoideae subfamily in the family Rosaceae.The self-incompatibility(SI)of some diploid species is a key agronomic trait that acts as a basic pollination barrier;however,the ...Strawberry(Fragaria spp.)is a member of the Rosoideae subfamily in the family Rosaceae.The self-incompatibility(SI)of some diploid species is a key agronomic trait that acts as a basic pollination barrier;however,the genetic mechanism underlying SI control in strawberry remains unclear.Two candidate S-RNases(S a-and S^-RNase)identi fi ed in the transcriptome of the styles of the self-incompatible Fragaria viridis 42 were con fi rmed to be SI determinants at the S locus following genotype identi fi cation and intraspeci fi c hybridization using sel fi ng progenies.Whole-genome collinearity and RNase T2 family analysis revealed that only an S locus exists in Fragaria;however,none of the compatible species contained S-RNase.Although the results of interspeci fi c hybridization experiments showed that F.viridis(SI)styles could accept pollen from F.mandshurica(self-compatible),the reciprocal cross was incompatible.S a and S b-RNase contain large introns,and their noncoding sequences(promotors and introns)can be transcribed into long noncoding RNAs(lncRNAs).Overall,the genus Fragaria exhibits S-RNase-based gametophytic SI,and S-RNase loss occurs at the S locus of compatible germplasms.In addition,a type of SI-independent unilateral incompatibility exists between compatible and incompatible Fragaria species.Furthermore,the large introns and neighboring lncRNAs in S-RNase in Fragaria could offer clues about S-RNase expression strategies.展开更多
The most practical high-temperature proton exchange membranes(PEMs) are phosphoric acid(PA)-doped polymer electrolytes. However, due to the plasticizing effect of PA, it is a challenge to address the trade-off between...The most practical high-temperature proton exchange membranes(PEMs) are phosphoric acid(PA)-doped polymer electrolytes. However, due to the plasticizing effect of PA, it is a challenge to address the trade-off between the proton conductivity and the mechanical performance of these materials. Here,we report an effective strategy to fabricate robust high-temperature PEMs based on the in situ electrostatic crosslinking of polyoxometalates and polymers. A comb copolymer poly(ether-ether-ketone)-grafted-poly(2-ethyl-2-oxazoline)(PGE) with transformable side chains was synthesized and complexed with H_(3)PW_(12)O_(40)(PW) by electrostatic self-assembly, forming PGE/PW nanocomposite membranes with bicontinuous nanostructures. After a subsequent PA-treatment of these membranes, high-temperature PEMs of PGE/PW/PA ternary nanocomposites were obtained, in which the in situ electrostatic crosslinking effect between PW and PGE side chains was generated in the hydrophilic domains of the bicontinuous structures. The microphase separation structure and the electrostatic crosslinking feature endow the PGE/PW/PA membranes with excellent anhydrous proton conductive ability while retaining high mechanical performance. The membranes show a high proton conductivity of 42.5 m S/cm at 150 ℃ and a high tensile strength of 13 MPa. Our strategy can pave a new route based on electrostatic control to design nanostructured polymer electrolytes.展开更多
The mechanisms of oxide metallurgy include inducing the formation of intragranular acicular ferrite(IAF)using micron-sized inclusions and restricting the growth of prior austenite grains(PAGs)by nanosized particles du...The mechanisms of oxide metallurgy include inducing the formation of intragranular acicular ferrite(IAF)using micron-sized inclusions and restricting the growth of prior austenite grains(PAGs)by nanosized particles during welding.The chaotically oriented IAF and refined PAGs inhibit crack initiation and propagation in the steel,resulting in high impact toughness.This work summarizes the com-bined effect of deoxidizers and alloying elements,with the aim to provide a new perspective for the research and practice related to im-proving the impact toughness of the heat affected zone(HAZ)during the high heat input welding.Ti complex deoxidation with other strong deoxidants,such as Mg,Ca,Zr,and rare earth metals(REMs),can improve the toughness of the heat-affected zone(HAZ)by re-fining PAGs or increasing IAF contents.However,it is difficult to identify the specific phase responsible for IAF nucleation because ef-fective inclusions formed by complex deoxidation are usually multiphase.Increasing alloying elements,such as C,Si,Al,Nb,or Cr,con-tents can impair HAZ toughness.A high C content typically increases the number of coarse carbides and decreases the potency of IAF formation.Si,Cr,or Al addition leads to the formation of undesirable microstructures.Nb reduces the high-temperature stability of the precipitates.Mo,V,and B can enhance HAZ toughness.Mo-containing precipitates present good thermal stability.VN or V(C,N)is ef-fective in promoting IAF nucleation due to its good coherent crystallographic relationship with ferrite.The formation of the B-depleted zone around the inclusion promotes IAF formation.The interactions between alloying elements are complex,and the effect of adding dif-ferent alloying elements remains to be evaluated.In the future,the interactions between various alloying elements and their effects on ox-ide metallurgy,as well as the calculation of the nucleation effects of effective inclusions using first principles calculations will become the focus of oxide metallurgy.展开更多
Lung cancer is the most common malignancy in the world, with a high mortality rate. Nevertheless,therapies to act effectively against lung cancer remain elusive. So far, chemotherapy is still the frontline treatment o...Lung cancer is the most common malignancy in the world, with a high mortality rate. Nevertheless,therapies to act effectively against lung cancer remain elusive. So far, chemotherapy is still the frontline treatment of lung cancer. Doxorubicin(DOX) is a broad-spectrum anti-tumor drug. However, DOX often has serious side effects and causes multi-drug resistance, which greatly limits its clinical application.In this work, biodegradable methoxy poly(ethylene glycol)-poly(lactic acid)(MPEG-PLA) and cyclo(ArgGly-Asp-D-Phe-Lys)(c RGD) polypeptide modified PEG-PLA(c RGD-PEG-PLA) copolymers were used for the co-delivery of curcumin(CUR) and DOX(CUR-DOX/c RGD-M). The particle size of the self-assembled drugloaded nanomicelle approximately was 27.4 nm and the zeta potential was -2.7mV. Interestingly, CUR can enhance the uptake of DOX by Lewis lung carcinoma(LL/2) cells. The experimental results in vivo and in vitro showed that CUR-DOX/c RGD-M combination therapy could promote apoptosis of lung cancer cells, and conspicuously inhibit the tumor growth. Our data indicate that CUR-DOX/c RGD-M will be biodegradable and sustainable, which may have potential clinical application value in the treatment of lung cancer.展开更多
An N-heterocyclic carbene(NHC)-catalyzed carbonyl nucleophilic substitution reaction between 1-cyclopropylcarbaldehydes and N-sulfonyl imines is developed for access to linearβ-aminoenone products.Theβ-aminoenones c...An N-heterocyclic carbene(NHC)-catalyzed carbonyl nucleophilic substitution reaction between 1-cyclopropylcarbaldehydes and N-sulfonyl imines is developed for access to linearβ-aminoenone products.Theβ-aminoenones containing cyclopropyl fragments can be afforded in moderate to excellent yields under mild conditions.The reaction features excellent trans-diastereoselectivities and the desired aminoenone products are all afforded as Z-isomers.展开更多
This paper considers the finite difference(FD)approximations of diffusion operators and the boundary treatments for different boundary conditions.The proposed schemes have the compact form and could achieve arbitrary ...This paper considers the finite difference(FD)approximations of diffusion operators and the boundary treatments for different boundary conditions.The proposed schemes have the compact form and could achieve arbitrary even order of accuracy.The main idea is to make use of the lower order compact schemes recursively,so as to obtain the high order compact schemes formally.Moreover,the schemes can be implemented efficiently by solving a series of tridiagonal systems recursively or the fast Fourier transform(FFT).With mathematical induction,the eigenvalues of the proposed differencing operators are shown to be bounded away from zero,which indicates the positive definiteness of the operators.To obtain numerical boundary conditions for the high order schemes,the simplified inverse Lax-Wendroff(SILW)procedure is adopted and the stability analysis is performed by the Godunov-Ryabenkii method and the eigenvalue spectrum visualization method.Various numerical experiments are provided to demonstrate the effectiveness and robustness of our algorithms.展开更多
Objective Mitochondrial reactive oxygen species(mtROS)could cause damage to pancreaticβ-cells,rendering them susceptible to oxidative damage.Hence,investigating the potential of the mitochondriatargeted antioxidant(M...Objective Mitochondrial reactive oxygen species(mtROS)could cause damage to pancreaticβ-cells,rendering them susceptible to oxidative damage.Hence,investigating the potential of the mitochondriatargeted antioxidant(Mito-TEMPO)to protect pancreaticβ-cells from ferroptosis by mitigating lipid peroxidation becomes crucial.Methods MIN6 cells were cultured in vitro with 100μmol/L sodium palmitate(SP)to simulate diabetes.FerroOrange was utilized for the detection of Fe2+fluorescence staining,BODIPY581/591C11 for lipid reactive oxygen species,and MitoSox-Red for mtROS.Alterations in mitophagy levels were assessed through the co-localization of lysosomal and mitochondrial fluorescence.Western blotting was employed to quantify protein levels of Acsl4,GPX4,FSP1,FE,PINK1,Parkin,TOMM20,P62,and LC3.Subsequently,interventions were implemented using Mito-TEMPO and Carbonyl cyanide 3-chlorophenylhydrazone(CCCP)to observe changes in ferroptosis and mitophagy within MIN6 cells.Results We found that SP induced a dose-dependent increase in Fe2+and lipid ROS in MIN6 cells while decreasing the expression levels of GPX4 and FSP1 proteins.Through bioinformatics analysis,it has been uncovered that mitophagy assumes a crucial role within the ferroptosis pathway associated with diabetes.Additionally,SP decreased the expression of mitophagy-related proteins PINK1 and Parkin,leading to mtROS overproduction.Conversely,Mito-TEMPO effectively eliminated mtROS while activating the mitophagy pathways involving PINK1 and Parkin,thereby reducing the occurrence of ferroptosis in MIN6 cells.CCCP also demonstrated efficacy in reducing ferroptosis in MIN6 cells.Conclusion In summary,Mito-TEMPO proved effective in attenuating mtROS production and initiating mitophagy pathways mediated by PINK1 and Parkin in MIN6 cells.Consequently,this decreased iron overload and lipid peroxidation,ultimately safeguarding the cells from ferroptosis.展开更多
A ternary complex combining dual-phase perovskites-Cs4PbBr6/CsPbBr3(DP-CPB) with ZnSe micropshere s(ZnSe-DP-CPB) was succes s fully prepared using supersaturated recrystallization technique at room temperature.It was ...A ternary complex combining dual-phase perovskites-Cs4PbBr6/CsPbBr3(DP-CPB) with ZnSe micropshere s(ZnSe-DP-CPB) was succes s fully prepared using supersaturated recrystallization technique at room temperature.It was showed that the DP-CPB composites were partially embedded in ZnSe microsphere composed with ZnSe NCs.The light absorption range of ZnSe-DP-CPB composites was extended from visible to near infrared light.Highly enhanced luminescence from ZnSe-DP-CPB composite was observed and the excitation power-dependent photoluminescence showed that the recombination involves excitons.The recombination lifetimes of the ternary composites increased compared with DP-CPB composite,indicating that the non-radiative combination was suppressed which maybe possibly due to the decrease of both bulk and surface defects,owing to the passivation of ZnSe,as well as the suitable band alignments of these three components.The ternary complex also showed improved stability of photoluminescence(PL),which opens a newavenue for enhancing the stability of PL and optoelectronic applications for semiconductor-perovskite composites.展开更多
基金supported by the China Postdoctoral Science Foundation(2021M701462)the National Natural Science Foundation of China(32072254)+2 种基金the Postdoctoral Research Funding Program of Jiangsu Province(2021K097A)the Fundamental Research Funds for the Central Universities(JUSRP121106)the“Qing Lan Project”of Jiangsu Province。
文摘The dark blue pigment(DBP)is a health ingredient from Vaccinium bracteatum Thunb.leaves used as a functional food supplement.However,the details of transepithelial absorption on the intestinal epithelial cells are barely understood.This study aimed to clarify the absorption properties of DBP in the Caco-2 cell monolayer model and evaluate the effect on the endo-metabolism and barrier function of Caco-2 cells.The results showed that the DBP did not show the dose-dependent toxic effect to Caco-2 cells between 0.25 and 1.5 mg/mL,which did not cause disorder in the normal cell metabolism and absorption activity.The Caco-2 cell monolayer model could absorb DBP by passive and active transport,and the absorptive pattern was dosedependent when the concentration was more than 0.25 mg/mL.During DBP absorption,an increase in m RNA and protein expressions of glucose transporters demonstrated that the glucose transporters were the potential transporter of DBP.But the glucose transport amounts were significantly lowered after 30 min of DBP treatment,indicating that DBP owned the inhibitory effect on glucose transportation.Furthermore,DBP also owned protective effects on the barrier function of intestinal epithelial cells.
基金financially supported by the National Natural Science Foundation of China(Nos.52201233,52371204,and 52031014).
文摘We report a systematic study on the transport properties of(Bi_(0.2)Sb_(0.8))_(2)Te_(3)and(Bi_(0.4)Sb_(0.6))_(2)Te_(3)nanoplates with a thickness of about 6 nm grown by chemical vapor deposition(CVD)on Si/SiO_(2)substrate.We achieve a significant ambipolar field effect in the two samples with different compositions by applying back-gate voltage,successfully tuning the Fermi level across the Dirac point of surface states.It is found that the Hall resistance exhibits strong non-linear behavior and magnetic field induced sign change of the slope when the Fermi level is near the Dirac point,indicating the coexistence of n-type and p-type carriers.Moreover,this coincides with the striking crossover from weak antilocalization(WAL)to linear magnetoresistance(LMR).These gate and temperature dependent magneto-transport studies provide a deeper insight into the nature of LMR and WAL in topological materials.
基金supported by the National Natural Science Foundation of China(42174115 and 42330311)the Special Fund of the Institute of Earthquake Forecasting,China Earthquake Administration(CEAIEF20230301)the State key laboratory of earthquake dynamics(LED2021B02).
文摘Structure and composition of Earth are fundamental importance in exploring the dynamic evolution of the crust and mantle.The Qinling Orogenic Belt(QOB)is located between the North China plate and the South China Plate,and is one of the main orogenic belts in China.To explore the composition and origin of anisotropy and the low wave velocity zone of the QOB,ten rock samples(gneiss and schist)were collected from the five sites of the QOB and the P-and S-wave velocities of these samples were measured under 0.6 to 2.0 GPa and 100 to 550℃.The wave velocities increase with increasing pressure and decreasing temperature.The V_(P)and V_(S)of the schist and gneiss match the velocity of the middle and lower crust of the QOB,indicating that schist and gneiss are important component of the QOB.All the schist and gneiss samples exhibit obvious seismic anisotropy with 1.64%-17.42%for V_(S)and 2.93%-14.78%for V_(P)under conditions of crust and upper mantle.The CPO/LPO and layering distribution of mica in rock samples are the main reasons for this anisotropy.The V_(S)structures below the five sampled sites from seismic ambient noise tomography were built to explore the effect of schist and gneiss on the composition and structure of the QOB.The results indicate that orientation-arranged gneiss and schist driven by the tectonic stresses might be a new origin of the character of V_(P)/V_(S),seismic anisotropy,and the low velocity zone in the QOB.
基金2025 College Students'Innovation and Entrepreneurship Training Plan Project:“Yinong Zhixing-Smart Agriculture Platform Helps Rural Revitalization”innovative research2024 College Students'Innovation and Entrepreneurship Training Plan Project:The Implementation of the Digital Economy in Promoting Rural Revitalization(Project number:202413207025)。
文摘With the continuous advancement of big data technology,its application in precision agriculture is becoming more and more extensive,which provides a new path for agricultural modernization.Based on the background of big data technology,this paper discusses the development path of precision agriculture.The research shows that the application of big data technology in precision agriculture is mainly reflected in crop growth monitoring and prediction,soil fertility assessment and regulation,water resources management and optimization,agricultural product traceability and origin tracing,and agricultural product supply chain management and risk control.However,the application of big data technology in the agricultural field stll encounters numerous challenges,such as data collection and processing,data analysis and application,and privacy protection and security.To this end,this paper puts forward countermeasures,including strengthening infrastructure construction and establishing data sharing and management mechanisms.
基金This work was jointly supported by the National Basic Research Program of China(2016YFA0602701)the National Natural Science Foundation of China(41975113)+1 种基金the Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies(2020B1212060025)the Guangdong Provincial Department of Science and Technology(2019ZT08G090)。
文摘“I call on all leaders worldwide to declare a State of Climate Emergency in their own countries until carbon neutrality is reached.”–António GUTERRES(United Nations Secretary General),12 December,2020 There is no shortcut to a carbon neutral society;solutions are urgently required from both energy&industrial sectors and global ecosystems.While the former is often held accountable and emphasized in terms of its emissions reduction capability,the latter(recently termed natural climate solutions)should also be assessed for potential and limitations by the scientific community,the public,and policy makers.
基金supported by the National Key Research and Development Program of China(Grant No.2016YFD0500800 to ZL)。
文摘H9N2 subtype avian influenza virus(AIV)is an influenza A virus that is widely spread throughout Asia,where it jeopardizes the poultry industry and provides genetic material for emerging human pathogens.To better understand the epidemicity and genetics of H9 subtype AIVs,we conducted active surveillance in live poultry markets(LPMs)in Hubei Province from 2013 to 2017.A total of 4798 samples were collected from apparent healthy poultry and environment.Realtime RT-PCR revealed that the positivity rate of influenza A was 26.6%(1275/4798),of which the H9 subtype accounted for 50.3%(641/1275)of the positive samples.Of the 132 H9N2 viral strains isolated,48 representative strains were subjected to evolutionary analysis and genotyping.Phylogenetic analysis revealed that all H9N2 viral genes had 91.1%–100%nucleotide homology,clustered with genotype 57,and had high homology with human H9N2 viruses isolated from2013 to 2017 in China.Using a nucleotide divergence cutoff of 95%,we identified ten distinct H9N2 genotypes that continued to change over time.Molecular analysis demonstrated that six H9N2 isolates had additional potential glycosylation sites at position 218 in the hemagglutinin protein,and all isolates had I155 T and Q226 L mutations.Moreover,44 strains had A558 V mutations in the PB2 protein and four had E627 V mutations,along with H9N2 human infection strains A/Beijing/1/2016 and A/Beijing/1/2017.These results emphasize that the H9N2 influenza virus in Hubei continues to mutate and undergo mammalian adaptation changes,indicating the necessity of strengthening the surveillance of the AIV H9N2 subtype in LPMs.
基金This work was supported by the National Natural Science Foundation of China(Nos.81901430 and 81871835)the Guangdong Provincial Natural Science Foundation of China(No.2022A1515010379)+1 种基金the Innovation Project from Department of Education of Guangdong Province(No.2021KTSCX 055)the Shanghai Frontiers Science Research Base of Exercise and Metabolic Health,and the Shanghai Key Laboratory for Human Athletic Ability Development and Support(Shanghai University of Sport)(No.11DZ2261100),China.
文摘Mammalian bone is constantly metabolized from the embryonic stage,and the maintenance of bone health depends on the dynamic balance between bone resorption and bone formation,mediated by osteoclasts and osteoblasts.It is widely recognized that circadian clock genes can regulate bone metabolism.In recent years,the regulation of bone metabolism by non-coding RNAs has become a hotspot of research.MicroRNAs can participate in bone catabolism and anabolism by targeting key factors related to bone metabolism,including circadian clock genes.However,research in this field has been conducted only in recent years and the mechanisms involved are not yet well established.Recent studies have focused on how to target circadian clock genes to treat some diseases,such as autoimmune diseases,but few have focused on the co-regulation of circadian clock genes and microRNAs in bone metabolic diseases.Therefore,in this paper we review the progress of research on the co-regulation of bone metabolism by circadian clock genes and microRNAs,aiming to provide new ideas for the prevention and treatment of bone metabolic diseases such as osteoporosis.
基金supported by grants from the Natural Science Foundation of Jiangsu Province (BL2013017)the Suzhou Science and Technology Bureau (SYS201156) to Dr. Feng Qian+1 种基金the Suzhou Health and Family Planning Commission (LCZX201413) to Ming Lithe Key National Science and Technology Program in the Thirteen Five-Year Plan Period of China (2017ZX10201102-007-002)
文摘Little is known about the prevalence of drug-resistant mutations in HIV-1-positive individuals in Suzhou, China. To elucidate the transmitted drug resistance(TDR) and acquired drug resistance mutation(ADR) profiles, we collected blood specimens from 127 drug-naive and 117 first-line drugtreated HIV-1-infected individuals sampled from 2014 to 2016 in Suzhou. We successfully amplified po/fragments from 100 drug-naive and 20 drug-treated samples. We then determined the drugresistant mutations to protease(PR) and reverse-transcriptase(RT) inhibitors according to the Stanford drug resistance database. Overall, 11 and 13 individuals had transmitted(drug-naive group) and acquired(treated group) resistance mutations, respectively. Six transmitted drugresistant mutations were found, including two mutations(L33F and L76V) in the protease region and four(K70N/E and V179D/E) in the RT region. Only L76 V was a major mutation, and K70N/E and V179D/E are known to cause low-level resistance to RT inhibitors. All 13 treated participants who had major drug resistance mutations demonstrated intermediate to high resistance to efavirenz and nevirapine, and six had a treatment duration of less than three months. No major mutations to RT inhibitors were found, implying that the epidemic of transmitted resistance mutations was not significant in this area. Our results suggest that more frequent virus load and drug resistance mutation tests should be conducted for individuals receiving antiretroviral treatment, especially for newly treated patients. Our research provides insights into the occurrence of HIV-1 drug resistance in Suzhou and will help to optimize the treatment strategy for this population.
基金supported by the National Natural Science Foundation of China(NSFC,No.51572011)the National Key Research and Development Program of China(No.2017YFB0102004)the Fundamental Research Funds for the Central Universities(No.ZY1802)
文摘Titanium oxide(TiO_(2)),with excellent cycling stability and low volume expansion,is a promising anode material for lithium-ion battery(LIB),which suffers from low electrical conductivity and poor rate capability.Combining nano-sized TiO_(2)with conductive materials is proved an efficient method to improve its electrochemical properties.Here,rutile TiO_(2)/carbon nanosheet was obtained by calcinating MAX(Ti_(3)AlC_(2))and Na_(2)C0_(3)together and water-bathing with HC1.The lamellar carbon atoms in MAX are converted to 2D carbon nanosheets with urchin-like rutile TiO_(2)anchored on.The unique architecture can offer plentiful active sites,shorten the ion diffusion distance and improve the conductivity.The composite exhibits a high reversible capacity of 247 mA h g^-1,excellent rate performance(38 mA h g^-1 at 50 C)and stable cycling performance(0.014%decay per cycle during 2000 cycles)for lithium storage.
基金supported by the grants from the Ministry of Science and Technology (2016YFA0501000)the National Science Foundation of China to YQZ (31490592) and AY (31271121)
文摘Mutations in long-chain acyl-CoA synthetase 4 (ACSL4) are associated with non-syndromic X-linked intellectual disability (ID). However, the neural functions of ACSL4 and how loss of ACSL4 leads to ID remain largely unexplored. We report here that mutations in Acsl, the Drosophila ortholog of human ACSL3 and ACSL4, result in developmental defects of the mushroom body (MB), the center of olfactory learning and memory. Specifically, Acsl mutants show fewer MB neuroblasts (Nbs) due to reduced proliferation activity and premature differentiation. Consistently, these surviving Nbs show reduced expression of cyclin E, a key regulator of the G1-to S-phase cell cycle transition, and nuclear mislocalization of the transcriptional factor Prospero, which is known to repress self-renewal genes and activate differentiating genes. Furthermore, RNA-seq analysis reveals downregulated Nb-and cell-cyclerelated genes and upregulated neuronal differentiation genes in Acsl mutant Nbs. As Drosophila Acsl and human ACSL4 are functionally conserved, our findings provide novel insights into a critical and previously unappreciated role of Acsl in neurogenesis and the pathogenesis of ACSL4-related ID.
文摘The potential geographic distribution and favorable climatic conditions of Machilus thunbergii under current and future predicted climates in China are predicted based on MaxEnt model and ArcGIS software. The results show that the AUC values in different time periods and emission seniors are more than 0.9, which indicates the prediction is excellent. Precipitation of the coldest quarter, precipitation of the driest month, annual precipitation, mean diurnal range, and temperature annual range are the most important environmental factors affecting the distribution of Machilus thunbergii. At present, the suitable areas of Machilus thunbergii are mainly concentrated in the eastern subtropics of China, with a total area of 118.47 × 104 km2. The medium-suitability area and the high-suitability area are concentrated in Wuyi Mountains, Luoxiao Mountains, Xuefeng Mountains, Nanling and east of Taiwan Mountains. With the change of climate, the suitable area increases, and the medium-suitability area and high-suitability area migrate and expand to the east, and the low-suitability area expands slightly to the west and north. So, the simulated distribution of Machilus thunbergii should be one of priorities, when instigating in-situ conservation. The research results can provide a theoretical reference for the popularization and planting of Machilus thunbergii.
基金financially supported by the National Natural Science Foundation of China(32102559)the Jiangsu Shuang Chuang Tuan Dui Program,China(JSSCTD202147)the Jiangsu Shuang Chuang Ren Cai Program,China(JSSCRC2021541)。
文摘Excessive abdominal fat deposition seriously restricts the production efficiency of broilers.Several studies found that dietary supplemental manganese(Mn)could effectively reduce the abdominal fat deposition of broilers,but the underlying mechanisms remain unclear.The present study aimed to investigate the effect of dietary supplementation with the inorganic or organic Mn on abdominal fat deposition,and enzyme activity and gene expression involved in lipid metabolism in the abdominal fat of male or female broilers.A total of 4201-d-old AA broilers(half males and half females)were randomly allotted by body weight and gender to 1 of 6 treatments with 10 replicates cages of 7 chicks per cage in a completely randomized design involving a 3(dietary Mn addition)×2(gender)factorial arrangement.Male or female broilers were fed with the Mn-unsupplemented basal diets containing 17.52 mg Mn kg^(-1)(d 1-21)and 15.62 mg Mn kg^(-1)(d 22-42)by analysis or the basal diets supplemented with 110 mg Mn kg^(-1)(d 1-21)and 80 mg Mn kg^(-1)(d 22-42)as either the Mn sulfate or the Mn proteinate with moderate chelation strength(Mn-Prot M)for 42 d.The results showed that the interaction between dietary Mn addition and gender had no impact(P>0.05)on any of the measured parameters;abdominal fat percentage of broilers was decreased(P<0.003)by Mn addition;Mn addition increased(P<0.004)adipose triglyceride lipase(ATGL)activity,while Mn-Prot M decreased(P<0.002)the fatty acid synthase(FAS)activity in the abdominal fat of broilers compared to the control;Mn addition decreased(P<0.009)diacylglycerol acyltransferase 2(DGAT2)mRNA expression level and peroxisome proliferator-activated receptor γ(PPARγ)mRNA and protein expression levels,but up-regulated(P<0.05)the ATGL mRNA and protein expression levels in the abdominal fat of broilers.It was concluded that dietary supplementation with Mn inhibited the abdominal fat deposition of broilers possibly via decreasing the expression of PPARγand DGAT2 as well as increasing the expression and activity of ATGL in the abdominal fat of broilers,and Mn-Prot M was more effective in inhibiting the FAS acitivity.
基金This work was supported by the National Natural Science Foundation of Chi na(Gr ant no.32072540,31872056)the Fun dame ntal Research Fun ds for the Central Universities(Grant no.KYZZ2021002)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘Strawberry(Fragaria spp.)is a member of the Rosoideae subfamily in the family Rosaceae.The self-incompatibility(SI)of some diploid species is a key agronomic trait that acts as a basic pollination barrier;however,the genetic mechanism underlying SI control in strawberry remains unclear.Two candidate S-RNases(S a-and S^-RNase)identi fi ed in the transcriptome of the styles of the self-incompatible Fragaria viridis 42 were con fi rmed to be SI determinants at the S locus following genotype identi fi cation and intraspeci fi c hybridization using sel fi ng progenies.Whole-genome collinearity and RNase T2 family analysis revealed that only an S locus exists in Fragaria;however,none of the compatible species contained S-RNase.Although the results of interspeci fi c hybridization experiments showed that F.viridis(SI)styles could accept pollen from F.mandshurica(self-compatible),the reciprocal cross was incompatible.S a and S b-RNase contain large introns,and their noncoding sequences(promotors and introns)can be transcribed into long noncoding RNAs(lncRNAs).Overall,the genus Fragaria exhibits S-RNase-based gametophytic SI,and S-RNase loss occurs at the S locus of compatible germplasms.In addition,a type of SI-independent unilateral incompatibility exists between compatible and incompatible Fragaria species.Furthermore,the large introns and neighboring lncRNAs in S-RNase in Fragaria could offer clues about S-RNase expression strategies.
基金financial support from the National Natural Science Foundation of China (No. 22075097)the Program for JLU Science and Technology Innovative Research Team (No. 2017TD-10)the Open Research Fund of State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences (No. 2020–09)。
文摘The most practical high-temperature proton exchange membranes(PEMs) are phosphoric acid(PA)-doped polymer electrolytes. However, due to the plasticizing effect of PA, it is a challenge to address the trade-off between the proton conductivity and the mechanical performance of these materials. Here,we report an effective strategy to fabricate robust high-temperature PEMs based on the in situ electrostatic crosslinking of polyoxometalates and polymers. A comb copolymer poly(ether-ether-ketone)-grafted-poly(2-ethyl-2-oxazoline)(PGE) with transformable side chains was synthesized and complexed with H_(3)PW_(12)O_(40)(PW) by electrostatic self-assembly, forming PGE/PW nanocomposite membranes with bicontinuous nanostructures. After a subsequent PA-treatment of these membranes, high-temperature PEMs of PGE/PW/PA ternary nanocomposites were obtained, in which the in situ electrostatic crosslinking effect between PW and PGE side chains was generated in the hydrophilic domains of the bicontinuous structures. The microphase separation structure and the electrostatic crosslinking feature endow the PGE/PW/PA membranes with excellent anhydrous proton conductive ability while retaining high mechanical performance. The membranes show a high proton conductivity of 42.5 m S/cm at 150 ℃ and a high tensile strength of 13 MPa. Our strategy can pave a new route based on electrostatic control to design nanostructured polymer electrolytes.
基金supported by the National Natural Science Foundation of China(No.U1960202).
文摘The mechanisms of oxide metallurgy include inducing the formation of intragranular acicular ferrite(IAF)using micron-sized inclusions and restricting the growth of prior austenite grains(PAGs)by nanosized particles during welding.The chaotically oriented IAF and refined PAGs inhibit crack initiation and propagation in the steel,resulting in high impact toughness.This work summarizes the com-bined effect of deoxidizers and alloying elements,with the aim to provide a new perspective for the research and practice related to im-proving the impact toughness of the heat affected zone(HAZ)during the high heat input welding.Ti complex deoxidation with other strong deoxidants,such as Mg,Ca,Zr,and rare earth metals(REMs),can improve the toughness of the heat-affected zone(HAZ)by re-fining PAGs or increasing IAF contents.However,it is difficult to identify the specific phase responsible for IAF nucleation because ef-fective inclusions formed by complex deoxidation are usually multiphase.Increasing alloying elements,such as C,Si,Al,Nb,or Cr,con-tents can impair HAZ toughness.A high C content typically increases the number of coarse carbides and decreases the potency of IAF formation.Si,Cr,or Al addition leads to the formation of undesirable microstructures.Nb reduces the high-temperature stability of the precipitates.Mo,V,and B can enhance HAZ toughness.Mo-containing precipitates present good thermal stability.VN or V(C,N)is ef-fective in promoting IAF nucleation due to its good coherent crystallographic relationship with ferrite.The formation of the B-depleted zone around the inclusion promotes IAF formation.The interactions between alloying elements are complex,and the effect of adding dif-ferent alloying elements remains to be evaluated.In the future,the interactions between various alloying elements and their effects on ox-ide metallurgy,as well as the calculation of the nucleation effects of effective inclusions using first principles calculations will become the focus of oxide metallurgy.
基金supported by the National Natural Science Foundation of China(No.82172630)the Key R&D Projects of the Science and Technology Department of Sichuan Province(Nos.2021YFS0237,2020YFS0213)the 1·3·5 Project for Disci-plines of Excellence,West China Hospital,Sichuan University(No.ZYJC21022).
文摘Lung cancer is the most common malignancy in the world, with a high mortality rate. Nevertheless,therapies to act effectively against lung cancer remain elusive. So far, chemotherapy is still the frontline treatment of lung cancer. Doxorubicin(DOX) is a broad-spectrum anti-tumor drug. However, DOX often has serious side effects and causes multi-drug resistance, which greatly limits its clinical application.In this work, biodegradable methoxy poly(ethylene glycol)-poly(lactic acid)(MPEG-PLA) and cyclo(ArgGly-Asp-D-Phe-Lys)(c RGD) polypeptide modified PEG-PLA(c RGD-PEG-PLA) copolymers were used for the co-delivery of curcumin(CUR) and DOX(CUR-DOX/c RGD-M). The particle size of the self-assembled drugloaded nanomicelle approximately was 27.4 nm and the zeta potential was -2.7mV. Interestingly, CUR can enhance the uptake of DOX by Lewis lung carcinoma(LL/2) cells. The experimental results in vivo and in vitro showed that CUR-DOX/c RGD-M combination therapy could promote apoptosis of lung cancer cells, and conspicuously inhibit the tumor growth. Our data indicate that CUR-DOX/c RGD-M will be biodegradable and sustainable, which may have potential clinical application value in the treatment of lung cancer.
基金the financial support from the National Natural Science Foundation of China(Nos.21772029,32172459,21961006,22001173)The 10 Talent Plan(Shicengci)of Guizhou Province(No.[2016]5649)+4 种基金the Science and Technology Department of Guizhou Province(Nos.[2019]1020,Qiankehejichu-ZK[2021]Key033)the Program of Introducing Talents of Discipline to Universities of China(111 Program,No.D20023)at Guizhou UniversityFrontiers Science Center for Asymmetric Synthesis and Medicinal Molecules,Department of Education,Guizhou Province[No.Qianjiaohe KY(2020)004]the Guizhou Province First-Class Disciplines Project(No.GNYL(2017)008)Guizhou University(China)。
文摘An N-heterocyclic carbene(NHC)-catalyzed carbonyl nucleophilic substitution reaction between 1-cyclopropylcarbaldehydes and N-sulfonyl imines is developed for access to linearβ-aminoenone products.Theβ-aminoenones containing cyclopropyl fragments can be afforded in moderate to excellent yields under mild conditions.The reaction features excellent trans-diastereoselectivities and the desired aminoenone products are all afforded as Z-isomers.
基金supported by the NSFC grant 11801143J.Lu’s research is partially supported by the NSFC grant 11901213+3 种基金the National Key Research and Development Program of China grant 2021YFA1002900supported by the NSFC grant 11801140,12171177the Young Elite Scientists Sponsorship Program by Henan Association for Science and Technology of China grant 2022HYTP0009the Program for Young Key Teacher of Henan Province of China grant 2021GGJS067.
文摘This paper considers the finite difference(FD)approximations of diffusion operators and the boundary treatments for different boundary conditions.The proposed schemes have the compact form and could achieve arbitrary even order of accuracy.The main idea is to make use of the lower order compact schemes recursively,so as to obtain the high order compact schemes formally.Moreover,the schemes can be implemented efficiently by solving a series of tridiagonal systems recursively or the fast Fourier transform(FFT).With mathematical induction,the eigenvalues of the proposed differencing operators are shown to be bounded away from zero,which indicates the positive definiteness of the operators.To obtain numerical boundary conditions for the high order schemes,the simplified inverse Lax-Wendroff(SILW)procedure is adopted and the stability analysis is performed by the Godunov-Ryabenkii method and the eigenvalue spectrum visualization method.Various numerical experiments are provided to demonstrate the effectiveness and robustness of our algorithms.
基金supported by a grant from the Science and Technology Tackling Programme Project of Xinjiang Production and Construction Corps(2021AB030).
文摘Objective Mitochondrial reactive oxygen species(mtROS)could cause damage to pancreaticβ-cells,rendering them susceptible to oxidative damage.Hence,investigating the potential of the mitochondriatargeted antioxidant(Mito-TEMPO)to protect pancreaticβ-cells from ferroptosis by mitigating lipid peroxidation becomes crucial.Methods MIN6 cells were cultured in vitro with 100μmol/L sodium palmitate(SP)to simulate diabetes.FerroOrange was utilized for the detection of Fe2+fluorescence staining,BODIPY581/591C11 for lipid reactive oxygen species,and MitoSox-Red for mtROS.Alterations in mitophagy levels were assessed through the co-localization of lysosomal and mitochondrial fluorescence.Western blotting was employed to quantify protein levels of Acsl4,GPX4,FSP1,FE,PINK1,Parkin,TOMM20,P62,and LC3.Subsequently,interventions were implemented using Mito-TEMPO and Carbonyl cyanide 3-chlorophenylhydrazone(CCCP)to observe changes in ferroptosis and mitophagy within MIN6 cells.Results We found that SP induced a dose-dependent increase in Fe2+and lipid ROS in MIN6 cells while decreasing the expression levels of GPX4 and FSP1 proteins.Through bioinformatics analysis,it has been uncovered that mitophagy assumes a crucial role within the ferroptosis pathway associated with diabetes.Additionally,SP decreased the expression of mitophagy-related proteins PINK1 and Parkin,leading to mtROS overproduction.Conversely,Mito-TEMPO effectively eliminated mtROS while activating the mitophagy pathways involving PINK1 and Parkin,thereby reducing the occurrence of ferroptosis in MIN6 cells.CCCP also demonstrated efficacy in reducing ferroptosis in MIN6 cells.Conclusion In summary,Mito-TEMPO proved effective in attenuating mtROS production and initiating mitophagy pathways mediated by PINK1 and Parkin in MIN6 cells.Consequently,this decreased iron overload and lipid peroxidation,ultimately safeguarding the cells from ferroptosis.
基金supported by National Natural Science Foundation of China(Nos.61927806,61474067)the State Key Laboratory of Transducer Technology of China(No.SKT1806)。
文摘A ternary complex combining dual-phase perovskites-Cs4PbBr6/CsPbBr3(DP-CPB) with ZnSe micropshere s(ZnSe-DP-CPB) was succes s fully prepared using supersaturated recrystallization technique at room temperature.It was showed that the DP-CPB composites were partially embedded in ZnSe microsphere composed with ZnSe NCs.The light absorption range of ZnSe-DP-CPB composites was extended from visible to near infrared light.Highly enhanced luminescence from ZnSe-DP-CPB composite was observed and the excitation power-dependent photoluminescence showed that the recombination involves excitons.The recombination lifetimes of the ternary composites increased compared with DP-CPB composite,indicating that the non-radiative combination was suppressed which maybe possibly due to the decrease of both bulk and surface defects,owing to the passivation of ZnSe,as well as the suitable band alignments of these three components.The ternary complex also showed improved stability of photoluminescence(PL),which opens a newavenue for enhancing the stability of PL and optoelectronic applications for semiconductor-perovskite composites.
