Although fungal communities in the gastrointestinal tract have a significant role in animal health and performance,their dynamics within the tract are not well known.Thus,this study investigated fungal community dynam...Although fungal communities in the gastrointestinal tract have a significant role in animal health and performance,their dynamics within the tract are not well known.Thus,this study investigated fungal community dynamics in the rumen and rectum of lambs from birth to 4 mon of age by using IT1S rDNA sequencing technology together with the RandomForest approach to determine age-related changes in the fungal ecology.The results indicated that gastrointestinal fungal community composition,diversity,and abundance altered(P<0.05)with the increasing age of the lambs.Two phyla,Ascomycota and Basidiomycota,dominated the samples.Similarity within age groups of the rumen fungi increased sharply after 45 days of age,while the similarity increased(P<0.05)significantly after 60 days of age in the rectum.The age-related genera,Acremonium,Microascus,Valsonectria,Myrmecridium,Scopulariopsis,Myrothecium,Saccharomyces,and Stephanonectria,were presented in both ruminal and rectal communities,and their changes in relative abundance were consistent at both sites.The principal coordinates analysis showed significant differences(P<0.05)between the fungal communities in the rumen and rectum.Our findings demonstrate that both the age of lambs and the gastrointestinal tract region can affect the composition of these fungal communities,and this provides new insight and directions for future studies in this research area.展开更多
Early bacterial colonization and succession within the gastrointestinal tract have been suggested to be crucial in the development of host immunity.In this study,we have investigated the changes in live weight and con...Early bacterial colonization and succession within the gastrointestinal tract have been suggested to be crucial in the development of host immunity.In this study,we have investigated the changes in live weight and concentrations of selected serum parameters in relation to their fecal bacterial communities as determined by high throughput sequencing of the 16S r RNA gene over the same period in lambs.The results showed that lambs’growth performance,the serum parameters,fecal bacterial community and fecal bacterial functions were all affected(P<0.05)by age of the lambs.Similarity within age groups of fecal microbiota was lower in the preweaning period and increased sharply(P<0.05)after weaning at 60 days.The similarity between the samples collected from birth to 90 days of age and those collected at 120 days of age,increased(P<0.05)sharply after 30 days of age.Some age-associated changes in microbial genera were correlated with the changes in concentrations of immune indicators,including negative(P<0.05)correlations between the relative abundance of Lachnospiraceae UCG-010,Eubacterium coprostanoligenes group,Ruminococcaceae UCG-005,Ruminococcaceae UCG-009,Ruminococcaceae UCG-013,Ruminiclostridium 6,Ruminococcaceae UCG-008,and Oscillibacter with serum concentrations of lipopolysaccharide(LPS),D-lactate dehydrogenase(DLA),immunoglobulin(IgA,IgM,and IgG),and cytokines(interleukin-1β(IL-1β),IL-6,IL-12,and IL-17),tumor necrosis factor-α(TNF-α),and the relative abundance of these genera increased from 45 days of age.In conclusion,these results suggested that the age-related abundances of particular genera were correlated with serum markers of immunity in lambs,and there might be a critical window in the period from birth to 45 days of age which provide an opportunity for potential manipulation of the fecal microbial ecosystems to enhance immune function.展开更多
基金supported by the China Agriculture Research System of MARA and MOF(CARS-38)the National Key R&D Program of China(2018YFD0502100)+1 种基金the Precision Animal Husbandry Discipline Group Construction Project of Hebei Agricultural University,China(1090064)the Scientific Research Foundation of Hebei Agricultural University,China(YJ201825)。
文摘Although fungal communities in the gastrointestinal tract have a significant role in animal health and performance,their dynamics within the tract are not well known.Thus,this study investigated fungal community dynamics in the rumen and rectum of lambs from birth to 4 mon of age by using IT1S rDNA sequencing technology together with the RandomForest approach to determine age-related changes in the fungal ecology.The results indicated that gastrointestinal fungal community composition,diversity,and abundance altered(P<0.05)with the increasing age of the lambs.Two phyla,Ascomycota and Basidiomycota,dominated the samples.Similarity within age groups of the rumen fungi increased sharply after 45 days of age,while the similarity increased(P<0.05)significantly after 60 days of age in the rectum.The age-related genera,Acremonium,Microascus,Valsonectria,Myrmecridium,Scopulariopsis,Myrothecium,Saccharomyces,and Stephanonectria,were presented in both ruminal and rectal communities,and their changes in relative abundance were consistent at both sites.The principal coordinates analysis showed significant differences(P<0.05)between the fungal communities in the rumen and rectum.Our findings demonstrate that both the age of lambs and the gastrointestinal tract region can affect the composition of these fungal communities,and this provides new insight and directions for future studies in this research area.
基金supported by the China Agriculture Research System(CARS-38)the National Key Research&Development Program of China(2018YFD0502100)the Scientific Research Foundation of Hebei Agricultural University,China(YJ201825)。
文摘Early bacterial colonization and succession within the gastrointestinal tract have been suggested to be crucial in the development of host immunity.In this study,we have investigated the changes in live weight and concentrations of selected serum parameters in relation to their fecal bacterial communities as determined by high throughput sequencing of the 16S r RNA gene over the same period in lambs.The results showed that lambs’growth performance,the serum parameters,fecal bacterial community and fecal bacterial functions were all affected(P<0.05)by age of the lambs.Similarity within age groups of fecal microbiota was lower in the preweaning period and increased sharply(P<0.05)after weaning at 60 days.The similarity between the samples collected from birth to 90 days of age and those collected at 120 days of age,increased(P<0.05)sharply after 30 days of age.Some age-associated changes in microbial genera were correlated with the changes in concentrations of immune indicators,including negative(P<0.05)correlations between the relative abundance of Lachnospiraceae UCG-010,Eubacterium coprostanoligenes group,Ruminococcaceae UCG-005,Ruminococcaceae UCG-009,Ruminococcaceae UCG-013,Ruminiclostridium 6,Ruminococcaceae UCG-008,and Oscillibacter with serum concentrations of lipopolysaccharide(LPS),D-lactate dehydrogenase(DLA),immunoglobulin(IgA,IgM,and IgG),and cytokines(interleukin-1β(IL-1β),IL-6,IL-12,and IL-17),tumor necrosis factor-α(TNF-α),and the relative abundance of these genera increased from 45 days of age.In conclusion,these results suggested that the age-related abundances of particular genera were correlated with serum markers of immunity in lambs,and there might be a critical window in the period from birth to 45 days of age which provide an opportunity for potential manipulation of the fecal microbial ecosystems to enhance immune function.
