旨在利用全基因组关联分析(genome-wide association study,GWAS)挖掘与鉴定吐鲁番黑羊体重和体尺性状的相关候选基因及分子标记,为吐鲁番黑羊的选育提高及资源开发利用提供科学依据。本研究选取129只年龄在1~3岁、饲养环境一致的健康...旨在利用全基因组关联分析(genome-wide association study,GWAS)挖掘与鉴定吐鲁番黑羊体重和体尺性状的相关候选基因及分子标记,为吐鲁番黑羊的选育提高及资源开发利用提供科学依据。本研究选取129只年龄在1~3岁、饲养环境一致的健康吐鲁番黑羊公羊,测定体重、体高、体斜长、胸围、管围、胸宽、胸深、尾宽、尾长共9项体重体尺性状表型数据;随后使用的一次性采血管(添加EDTA-K2抗凝剂)采集5 mL颈静脉外周血提取DNA。对合格的DNA样本进行简化基因组测序(genotyping-by-sequencing,GBS),运用samtools、bcftools等软件对原始测序数据进行检测、过滤及功能注释;然后利用GCTA、TreeBeST、GEMMA等软件分别完成群体遗传结构分析、群体分层评估及全基因组关联分析。本研究测序得到160.88 G的Raw data,质控后Clean data为157.87 G,获得460766个SNPs位点。全基因关联分析在9个性状中共筛选到74个显著SNPs位点(P<10-5),进一步注释得到到39个候选基因。单倍型分析发现候选基因的多个显著位点均位于单倍型block区域内。本研究鉴定了与吐鲁番黑羊体重体尺性状显著关联的SNP位点及候选基因,为深入解析这些性状的遗传机制提供了重要依据,同时也为吐鲁番黑羊分子标记辅助育种提供了基础数据。展开更多
The modifiedλ-differential Lie-Yamaguti algebras are considered,in which a modifiedλ-differential Lie-Yamaguti algebra consisting of a Lie-Yamaguti algebra and a modifiedλ-differential operator.First we introduce t...The modifiedλ-differential Lie-Yamaguti algebras are considered,in which a modifiedλ-differential Lie-Yamaguti algebra consisting of a Lie-Yamaguti algebra and a modifiedλ-differential operator.First we introduce the representation of modifiedλ-differential Lie-Yamaguti algebras.Furthermore,we establish the cohomology of a modifiedλ-differential Lie-Yamaguti algebra with coefficients in a representation.Finally,we investigate the one-parameter formal deformations and Abelian extensions of modifiedλ-differential Lie-Yamaguti algebras using the second cohomology group.展开更多
Biological soil disinfestation is an effective method to control soil-borne disease by flooding and incorporating with organic amendments, but field conditions and resources sometimes limited its practical application...Biological soil disinfestation is an effective method to control soil-borne disease by flooding and incorporating with organic amendments, but field conditions and resources sometimes limited its practical application. A laboratory experiment was conducted to develop practice guidelines on controlling Fusarium wilt, a widespread banana disease caused by Fusarium oxysporum f. sp. cubense（FOC）. FOC infested soil incorporated with rice or maize straw at rates of 1.5 tons/ha and 3.0 tons/ha was incubated under flooded or water-saturated（100% water holding capacity） conditions at 30℃ for 30 days. Results showed that FOC populations in the soils incorporated with either rice or maize straw rapidly reduced more than 90% in the first 15 days and then fluctuated till the end of incubation, while flooding alone without organic amendment reduced FOC populations slightly. The rapid and dramatic decrease of redox potential（down to- 350 m V） in straw-amended treatments implied that both anaerobic condition and strongly reductive soil condition would contribute to pathogen inactivation. Water-saturation combined with straw amendments had the comparable effects on reduction of FOC, indicating that flooding was not indispensable for inactivating FOC. There was no significant difference in the reduction of FOC observed in the straw amendments at between 1.5 and 3 tons/ha. Therefore,incorporating soil with straw（rice or maize straw） at a rate of 3.0 tons/ha under 100%water holding capacity or 1.5 tons/ha under flooding, would effectively alleviate banana Fusarium wilt caused by FOC after 15-day treating under 30℃.展开更多
Molluscan shell color has received persistent attention for its distinctive diversity and complexity. In the present study, six transcriptome libraries obtained from two developmental stages, pre-pigmentation and post...Molluscan shell color has received persistent attention for its distinctive diversity and complexity. In the present study, six transcriptome libraries obtained from two developmental stages, pre-pigmentation and post-pigmentation, were used for paired-end sequencing in the bay scallop Argopecten irradians. In total, 289 839 646 paired-end reads were assembled into 70 929 transcripts. Using BLASTX and BLASTN, 30 896 unigenes were successfully annotated against the SWISS-PROT, NR, and KOG database. Gene ontology annotation and Kyoto Encyclopedia of Genes and Genomes classification identified numbers of unigenes involved in biomineralization and pigmentation. Digital gene expression analysis revealed that melanin, trace metal elements and porphyrins are potentially involved in shell coloration of A. irradians.展开更多
基金National Natural Science Foundation of China(12161013)Research Projects of Guizhou University of Commerce in 2024。
文摘The modifiedλ-differential Lie-Yamaguti algebras are considered,in which a modifiedλ-differential Lie-Yamaguti algebra consisting of a Lie-Yamaguti algebra and a modifiedλ-differential operator.First we introduce the representation of modifiedλ-differential Lie-Yamaguti algebras.Furthermore,we establish the cohomology of a modifiedλ-differential Lie-Yamaguti algebra with coefficients in a representation.Finally,we investigate the one-parameter formal deformations and Abelian extensions of modifiedλ-differential Lie-Yamaguti algebras using the second cohomology group.
基金supported by the National Natural Science Foundation of China (Nos. 41222005, 41330744, 413301335)the Natural Science Foundation of Jiangsu Province (Nos. BK2010611, SBK201220477)+1 种基金Research Fund of State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences (Y412201404)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Biological soil disinfestation is an effective method to control soil-borne disease by flooding and incorporating with organic amendments, but field conditions and resources sometimes limited its practical application. A laboratory experiment was conducted to develop practice guidelines on controlling Fusarium wilt, a widespread banana disease caused by Fusarium oxysporum f. sp. cubense（FOC）. FOC infested soil incorporated with rice or maize straw at rates of 1.5 tons/ha and 3.0 tons/ha was incubated under flooded or water-saturated（100% water holding capacity） conditions at 30℃ for 30 days. Results showed that FOC populations in the soils incorporated with either rice or maize straw rapidly reduced more than 90% in the first 15 days and then fluctuated till the end of incubation, while flooding alone without organic amendment reduced FOC populations slightly. The rapid and dramatic decrease of redox potential（down to- 350 m V） in straw-amended treatments implied that both anaerobic condition and strongly reductive soil condition would contribute to pathogen inactivation. Water-saturation combined with straw amendments had the comparable effects on reduction of FOC, indicating that flooding was not indispensable for inactivating FOC. There was no significant difference in the reduction of FOC observed in the straw amendments at between 1.5 and 3 tons/ha. Therefore,incorporating soil with straw（rice or maize straw） at a rate of 3.0 tons/ha under 100%water holding capacity or 1.5 tons/ha under flooding, would effectively alleviate banana Fusarium wilt caused by FOC after 15-day treating under 30℃.
基金Supported by the China Agriculture Research System(No.CARS-49)the Science and Technology Service Network Initiative of Chinese Academy of Sciences
文摘Molluscan shell color has received persistent attention for its distinctive diversity and complexity. In the present study, six transcriptome libraries obtained from two developmental stages, pre-pigmentation and post-pigmentation, were used for paired-end sequencing in the bay scallop Argopecten irradians. In total, 289 839 646 paired-end reads were assembled into 70 929 transcripts. Using BLASTX and BLASTN, 30 896 unigenes were successfully annotated against the SWISS-PROT, NR, and KOG database. Gene ontology annotation and Kyoto Encyclopedia of Genes and Genomes classification identified numbers of unigenes involved in biomineralization and pigmentation. Digital gene expression analysis revealed that melanin, trace metal elements and porphyrins are potentially involved in shell coloration of A. irradians.
