A lesion mimic stripe mutant, designated as Ims1 （lesion mimic stripe 1）, was obtained from the M2 progeny of a ^60Co y-radiation treated japonica rice variety Jiahua 1. The Ims1 mutant displayed propagation type le...A lesion mimic stripe mutant, designated as Ims1 （lesion mimic stripe 1）, was obtained from the M2 progeny of a ^60Co y-radiation treated japonica rice variety Jiahua 1. The Ims1 mutant displayed propagation type lesions across the whole growth and developmental stages. Physiology and histochemistry analysis showed that the mutant exhibited a phenotype of white stripe when grown under high temperature （30 ℃）, and the lesion mimic caused by programmed cell death under low temperature （20 ℃）. The genetic analysis indicated that this lesion-mimic phenotype is controlled by a single locus recessive nuclear gene. Furthermore, by using simple sequence repeat markers and an F2 segregating population derived from two crosses of Ims1 ×93-11 and Ims1 ×Pei＇ai 64S, the Imsl gene was mapped between markers Indel1 and MM0112-4 with a physical distance of 400 kb on chromosome 6 in rice.展开更多
A double haploid population, derived from anther culture of F, hybrid between a typical indica and a japonica (ZYQ8/JX17), has been used to investigate the low temperature germinability (LTG) at 15℃. The low temperat...A double haploid population, derived from anther culture of F, hybrid between a typical indica and a japonica (ZYQ8/JX17), has been used to investigate the low temperature germinability (LTG) at 15℃. The low temperature germinability of two parents was significantly different. In 6-11 d, the germination percentage of ZYQ8 was higher than that of JX17. In 12-16 d, the germination percentage of JX17 was higher than that of ZYQ8. The quantitative trait loci (QTLs) of every day for low temperature germinabilityhave been mapped based on a molecular linkage map constructed from this population. In 8-11 d, qLTG-9 was identified in C397B-RZ617B on chromosome 9, the additive effect was positive, showing that, the allele from JX17 could increase low temperature germinability. In 12-16 d, qLTG-4 was mapped between RG908 and CT563 on chromosome 4, the additive effect was negative, showing that the allele from ZYQ8 could increase low temperature germinability. These two QTLs were detected at different stages,展开更多
The fragile rice mutant was isolated from an M2 population of indica variety Shuang Ke Zao (SKZ) treated with γ-rays, and designated as fp1 {fragile plant 1) because of its fragile leaves and culms. To map FP1 locus,...The fragile rice mutant was isolated from an M2 population of indica variety Shuang Ke Zao (SKZ) treated with γ-rays, and designated as fp1 {fragile plant 1) because of its fragile leaves and culms. To map FP1 locus, an F2 mapping population was derived from a cross between the fp1 and C-bao, a polymorphic japonic variety. The primary mapping result places the FP1 locus in an interval between two molecular markers, microsatellite marker RM16 (3.1 cM proximal to FP1) and STS marker G144a (9.1 cM distal to FP1) in the centromere region of chromosome 3. A CAPS marker C524a was further developed between RM16 and G144a, with 0.4 cM genetic distances to the FP1 locus, providing a practical starting point for constructing a BAC con-tig spanning the FP1 locus and cloning the fp1 gene. Allelism test demonstrated that fpl is allelic to be 1, a fragile rice mutant reported previously.展开更多
Background It is important to analyze and compare soft tissue sarcomas periodically so as to update the incidence, the clinical diagnosis, the treatment, and the ongoing research. The present study was conducted to de...Background It is important to analyze and compare soft tissue sarcomas periodically so as to update the incidence, the clinical diagnosis, the treatment, and the ongoing research. The present study was conducted to determine the relative frequency of each type of soft tissue sarcoma.Methods A total of 1118 cases of primary soft tissue sarcomas treated between January 1993 and December 2006 were evaluated in a retrospective analysis.Results According to the pathologic grouping, the diseases with the highest proportion were malignant fibrous histiocytomas （35.24%）, synovial sarcomas （17.08%）, liposarcomas （16.28%）, and rhabdomyosarcomas （12.61%）. Soft tissue sarcomas were detected in every age group and occurred in all parts of the body. The number of cases increased gradually over the years.Conclusions Malignant fibrous histiocytomas had the highest frequency among the soft tissue sarcomas. The number of cases increased gradually over the years.展开更多
基金supported by the National Basic Research Program of China (Grant No. 2009CB119000)the National Science Foundation of China (Grant Nos.31000094,31100188 and 30970246)
文摘A lesion mimic stripe mutant, designated as Ims1 （lesion mimic stripe 1）, was obtained from the M2 progeny of a ^60Co y-radiation treated japonica rice variety Jiahua 1. The Ims1 mutant displayed propagation type lesions across the whole growth and developmental stages. Physiology and histochemistry analysis showed that the mutant exhibited a phenotype of white stripe when grown under high temperature （30 ℃）, and the lesion mimic caused by programmed cell death under low temperature （20 ℃）. The genetic analysis indicated that this lesion-mimic phenotype is controlled by a single locus recessive nuclear gene. Furthermore, by using simple sequence repeat markers and an F2 segregating population derived from two crosses of Ims1 ×93-11 and Ims1 ×Pei＇ai 64S, the Imsl gene was mapped between markers Indel1 and MM0112-4 with a physical distance of 400 kb on chromosome 6 in rice.
基金This work was supported by the National Natural Science Foundation of China (Grant No. 39970471) and the Natural Science Foundation of Zhejiang Province (Grant No. 300220).
文摘A double haploid population, derived from anther culture of F, hybrid between a typical indica and a japonica (ZYQ8/JX17), has been used to investigate the low temperature germinability (LTG) at 15℃. The low temperature germinability of two parents was significantly different. In 6-11 d, the germination percentage of ZYQ8 was higher than that of JX17. In 12-16 d, the germination percentage of JX17 was higher than that of ZYQ8. The quantitative trait loci (QTLs) of every day for low temperature germinabilityhave been mapped based on a molecular linkage map constructed from this population. In 8-11 d, qLTG-9 was identified in C397B-RZ617B on chromosome 9, the additive effect was positive, showing that, the allele from JX17 could increase low temperature germinability. In 12-16 d, qLTG-4 was mapped between RG908 and CT563 on chromosome 4, the additive effect was negative, showing that the allele from ZYQ8 could increase low temperature germinability. These two QTLs were detected at different stages,
基金This work was supported by the State Key Basic Research Program (Grant No. 19990116) the National Natural Science Foundation of China (Grant Nos. 39970409 and 39970871) the Natural Science Foundation of Zhejiang Province (Grant Nos. 300220 and 300221
文摘The fragile rice mutant was isolated from an M2 population of indica variety Shuang Ke Zao (SKZ) treated with γ-rays, and designated as fp1 {fragile plant 1) because of its fragile leaves and culms. To map FP1 locus, an F2 mapping population was derived from a cross between the fp1 and C-bao, a polymorphic japonic variety. The primary mapping result places the FP1 locus in an interval between two molecular markers, microsatellite marker RM16 (3.1 cM proximal to FP1) and STS marker G144a (9.1 cM distal to FP1) in the centromere region of chromosome 3. A CAPS marker C524a was further developed between RM16 and G144a, with 0.4 cM genetic distances to the FP1 locus, providing a practical starting point for constructing a BAC con-tig spanning the FP1 locus and cloning the fp1 gene. Allelism test demonstrated that fpl is allelic to be 1, a fragile rice mutant reported previously.
文摘Background It is important to analyze and compare soft tissue sarcomas periodically so as to update the incidence, the clinical diagnosis, the treatment, and the ongoing research. The present study was conducted to determine the relative frequency of each type of soft tissue sarcoma.Methods A total of 1118 cases of primary soft tissue sarcomas treated between January 1993 and December 2006 were evaluated in a retrospective analysis.Results According to the pathologic grouping, the diseases with the highest proportion were malignant fibrous histiocytomas （35.24%）, synovial sarcomas （17.08%）, liposarcomas （16.28%）, and rhabdomyosarcomas （12.61%）. Soft tissue sarcomas were detected in every age group and occurred in all parts of the body. The number of cases increased gradually over the years.Conclusions Malignant fibrous histiocytomas had the highest frequency among the soft tissue sarcomas. The number of cases increased gradually over the years.
