Positional information plays a crucial role in embryonic pattern formation,yet its role in tooth development remains unexplored.In this study,we investigated the regional specification of lingual and buccal dental mes...Positional information plays a crucial role in embryonic pattern formation,yet its role in tooth development remains unexplored.In this study,we investigated the regional specification of lingual and buccal dental mesenchyme during tooth development.Tooth germs at the cap stage were dissected from mouse mandibles,and their lingual and buccal mesenchymal regions were separated for bulk RNA sequencing.Gene ontology analysis revealed that odontogenesis,pattern specification,and proliferation-related genes were enriched in the lingual mesenchyme,whereas stem cell development,mesenchymal differentiation,neural crest differentiation,and regeneration-related genes were predominant in the buccal mesenchyme.Reaggregation experiments using Wnt1^(cre ERT/+);R26R^(td T/+)and WT mouse models demonstrated that lingual mesenchyme contributes to tooth formation,while buccal mesenchyme primarily supports surrounding tissues.Furthermore,only the lingual part of tooth germs exhibited odontogenic potential when cultured in vitro and transplanted under the kidney capsule.Bulk RNA transcriptomic analysis further validated the regional specification of the lingual and buccal mesenchyme.These findings provide novel insights into the molecular basis of positional information in tooth development and pattern formation.展开更多
Photosynthesis, enzyme activities and metabolite pools associated with primary carbon metabolism in leaves were studied in O. grandiglumis and O. alta （wild relatives of rice which produce high biomass） versus O. sa...Photosynthesis, enzyme activities and metabolite pools associated with primary carbon metabolism in leaves were studied in O. grandiglumis and O. alta （wild relatives of rice which produce high biomass） versus O. sativa （a japonica cultivar and a indica-japonica hybrid） to assess their potential for identifying traits which might be utilized to enhance rice productivity. The wild relatives had higher rates of photosynthesis on a fresh weight basis, and higher water use efficiency than the O. sativa lines. There were no striking differences in activities of a number of key enzymes in carbon and nitrogen metabolism between the wild relatives and cultivated rice lines. Along with higher rates of photosynthesis on a fresh weight basis, the leaves of the two wild species had higher nitrate content, higher levels of starch, glucose and fructose, and higher levels of organic acids （malate, succinate and acetate）, compared to the O. sativa lines. The results suggested that O. grandiglumis and O. alta have differences in physiology and primary metabolism which might be exploited to improve growth and productivity of cultivated rice.展开更多
基金The National Research Foundation of Korea(NRF)Grant funded by the Korea Government(MSIP)(RS-2024-00459728,RS-2025-00553972)supported this work。
文摘Positional information plays a crucial role in embryonic pattern formation,yet its role in tooth development remains unexplored.In this study,we investigated the regional specification of lingual and buccal dental mesenchyme during tooth development.Tooth germs at the cap stage were dissected from mouse mandibles,and their lingual and buccal mesenchymal regions were separated for bulk RNA sequencing.Gene ontology analysis revealed that odontogenesis,pattern specification,and proliferation-related genes were enriched in the lingual mesenchyme,whereas stem cell development,mesenchymal differentiation,neural crest differentiation,and regeneration-related genes were predominant in the buccal mesenchyme.Reaggregation experiments using Wnt1^(cre ERT/+);R26R^(td T/+)and WT mouse models demonstrated that lingual mesenchyme contributes to tooth formation,while buccal mesenchyme primarily supports surrounding tissues.Furthermore,only the lingual part of tooth germs exhibited odontogenic potential when cultured in vitro and transplanted under the kidney capsule.Bulk RNA transcriptomic analysis further validated the regional specification of the lingual and buccal mesenchyme.These findings provide novel insights into the molecular basis of positional information in tooth development and pattern formation.
基金support of Cooperative Research Program for Agriculture Science&Technology Development(Grant No.PJ010899),Rural Development Administration,Republic of Korea
文摘Photosynthesis, enzyme activities and metabolite pools associated with primary carbon metabolism in leaves were studied in O. grandiglumis and O. alta （wild relatives of rice which produce high biomass） versus O. sativa （a japonica cultivar and a indica-japonica hybrid） to assess their potential for identifying traits which might be utilized to enhance rice productivity. The wild relatives had higher rates of photosynthesis on a fresh weight basis, and higher water use efficiency than the O. sativa lines. There were no striking differences in activities of a number of key enzymes in carbon and nitrogen metabolism between the wild relatives and cultivated rice lines. Along with higher rates of photosynthesis on a fresh weight basis, the leaves of the two wild species had higher nitrate content, higher levels of starch, glucose and fructose, and higher levels of organic acids （malate, succinate and acetate）, compared to the O. sativa lines. The results suggested that O. grandiglumis and O. alta have differences in physiology and primary metabolism which might be exploited to improve growth and productivity of cultivated rice.
