The rapid degradation of Mg alloy was reduced by incorporating titanate on a fluorine-based anodized layer.The coating shows(i)excellent biomineralization ability,(ii)improved local and periodical corrosion behavior a...The rapid degradation of Mg alloy was reduced by incorporating titanate on a fluorine-based anodized layer.The coating shows(i)excellent biomineralization ability,(ii)improved local and periodical corrosion behavior and(iii)enhanced expression of osteogenic factors(Runx2,Col 1,OCN and OPN)along with(iv)the antibacterial property.The fluoride and magnesium ions dissolution from the anodized layer is responsible for the better expression of osteogenic factors and antibacterial behavior.The preparation of the titanate incorporated anodized Mg alloy(Ti-AMg)is a facile solution to overcome the implant-associated bacterial infections with required biological functions including progression of bone ingrowth and biocompatibility.展开更多
Objective:To investigate the effects of Gymnema montanum leaf extract against endoplasmic reticulum(ER)stress-induced toxicity in endothelial cells.Methods:The immortalized endothelial hybrid cell,EA.hy926 was treated...Objective:To investigate the effects of Gymnema montanum leaf extract against endoplasmic reticulum(ER)stress-induced toxicity in endothelial cells.Methods:The immortalized endothelial hybrid cell,EA.hy926 was treated with different concentrations of Gymnema montanum leaf extract(0–100μg/mL)and the ER stress inducer,tunicamycin.The cytotoxicity was assessed by MTT as well as lactate dehydrogenase and malondialdehyde levels were determined.The levels of ER stress markers,GRP78 and CHOP were analysed by Western blot assay.The Gymnema montanum leaf extract-mediated activation of nuclear factor erythroid 2-related factor 2(Nrf2)was assessed by cell-based luciferase enzyme fragment complementation assay and antioxidant responsive element driven luciferase reporter assay.The levels of phosphoproteins of the MAPK pathway were analyzed using the Bioplex system.Results:A dose-dependent cytoprotective effect of Gymnema montanum leaf extract was observed in tunicamycin-induced toxicity.Gymnema montanum leaf extract significantly reduced lactate dehydrogenase activity and malondialdehyde levels in ER stress-induced endothelial cells.It also suppressed ER stress markers dose dependently and inhibited the phosphorylation of JNK,ERK,MEK and p38 MAPK in tunicamycin-induced endothelial cells.Moreover,Gymnema montanum leaf extract increased the expression of Nrf2 and its downstream targets in endothelial cells.Conclusions:Gymnema montanum leaf extract attenuates ER stress by increasing the expression of Nrf2 and its downstream genes.展开更多
The current scenario in tissue engineering research demands materials of requisite properties, viz., high porosity, mechanical stability, thermal stability, biocompatibility and biodegradability for clinical applicati...The current scenario in tissue engineering research demands materials of requisite properties, viz., high porosity, mechanical stability, thermal stability, biocompatibility and biodegradability for clinical applications. However, bringing these properties in single biomaterial is a challenging task, which needs intensive research on suitable cross-linking agents. In the present study, 3D scaffold was prepared with above said properties using chitosan and oxalic (O), malonic (M), succinic (S), glutaric (G), adipic (A), pimelic (P), suberic (SU), azelaic (AZ) and sebacic (SE) acid (OMS- GAP-SAS) individually as a non covalent cross-linkers as well as the solvent for chitosan. Assessment on degree of cross-linking, mechanical strength, FT-IR analysis, morphological observation, thermal stability, binding interactions (molecular docking), in vitro biocompatibility and its efficacy as a wound dressing material were performed. Results revealed the degree of cross-linking for OMSGAP-SAS engineered chitosan were in the range between ≈55% - 65% and the biomaterial demonstrated thermal stability more than 300°C and also exhibited ≥3 - 4 fold increase in mechanical strength compared to chitosan alone. The bioinformatics studies evidently proved the chemistry behind the interaction of OMSGAP-SAS with chitosan. OMSGAP-SAS played dual role to develop the chitosan biomaterial with above said properties, thus matching the requirements needed for various applications.展开更多
基金One of the authors K.Saranya is thankful to the Department of Science and Technology(DST),New Delhi,India for financial assistance under women scientist scheme(WOS-A)(Ref.No:SR/WOS-A/ET-17/2017).
文摘The rapid degradation of Mg alloy was reduced by incorporating titanate on a fluorine-based anodized layer.The coating shows(i)excellent biomineralization ability,(ii)improved local and periodical corrosion behavior and(iii)enhanced expression of osteogenic factors(Runx2,Col 1,OCN and OPN)along with(iv)the antibacterial property.The fluoride and magnesium ions dissolution from the anodized layer is responsible for the better expression of osteogenic factors and antibacterial behavior.The preparation of the titanate incorporated anodized Mg alloy(Ti-AMg)is a facile solution to overcome the implant-associated bacterial infections with required biological functions including progression of bone ingrowth and biocompatibility.
基金supported by the Indian Council of Medical Research(grant no.59/57/2011/BMS/TRM),Government of India
文摘Objective:To investigate the effects of Gymnema montanum leaf extract against endoplasmic reticulum(ER)stress-induced toxicity in endothelial cells.Methods:The immortalized endothelial hybrid cell,EA.hy926 was treated with different concentrations of Gymnema montanum leaf extract(0–100μg/mL)and the ER stress inducer,tunicamycin.The cytotoxicity was assessed by MTT as well as lactate dehydrogenase and malondialdehyde levels were determined.The levels of ER stress markers,GRP78 and CHOP were analysed by Western blot assay.The Gymnema montanum leaf extract-mediated activation of nuclear factor erythroid 2-related factor 2(Nrf2)was assessed by cell-based luciferase enzyme fragment complementation assay and antioxidant responsive element driven luciferase reporter assay.The levels of phosphoproteins of the MAPK pathway were analyzed using the Bioplex system.Results:A dose-dependent cytoprotective effect of Gymnema montanum leaf extract was observed in tunicamycin-induced toxicity.Gymnema montanum leaf extract significantly reduced lactate dehydrogenase activity and malondialdehyde levels in ER stress-induced endothelial cells.It also suppressed ER stress markers dose dependently and inhibited the phosphorylation of JNK,ERK,MEK and p38 MAPK in tunicamycin-induced endothelial cells.Moreover,Gymnema montanum leaf extract increased the expression of Nrf2 and its downstream targets in endothelial cells.Conclusions:Gymnema montanum leaf extract attenuates ER stress by increasing the expression of Nrf2 and its downstream genes.
文摘The current scenario in tissue engineering research demands materials of requisite properties, viz., high porosity, mechanical stability, thermal stability, biocompatibility and biodegradability for clinical applications. However, bringing these properties in single biomaterial is a challenging task, which needs intensive research on suitable cross-linking agents. In the present study, 3D scaffold was prepared with above said properties using chitosan and oxalic (O), malonic (M), succinic (S), glutaric (G), adipic (A), pimelic (P), suberic (SU), azelaic (AZ) and sebacic (SE) acid (OMS- GAP-SAS) individually as a non covalent cross-linkers as well as the solvent for chitosan. Assessment on degree of cross-linking, mechanical strength, FT-IR analysis, morphological observation, thermal stability, binding interactions (molecular docking), in vitro biocompatibility and its efficacy as a wound dressing material were performed. Results revealed the degree of cross-linking for OMSGAP-SAS engineered chitosan were in the range between ≈55% - 65% and the biomaterial demonstrated thermal stability more than 300°C and also exhibited ≥3 - 4 fold increase in mechanical strength compared to chitosan alone. The bioinformatics studies evidently proved the chemistry behind the interaction of OMSGAP-SAS with chitosan. OMSGAP-SAS played dual role to develop the chitosan biomaterial with above said properties, thus matching the requirements needed for various applications.
