Introduction:This study aimed to establish a robust method for monitoring measles vaccine-induced immunity and assessing population-level serostatus.Methods:This study constructed a vesicular stomatitis virus(VSV)-bas...Introduction:This study aimed to establish a robust method for monitoring measles vaccine-induced immunity and assessing population-level serostatus.Methods:This study constructed a vesicular stomatitis virus(VSV)-based pseudotyped virus system expressing envelope proteins from seven major circulating measles genotypes(H1,B3,D4,D8,D9,D11,G3)and the Schwarz vaccine strain(genotype A),thereby enabling a high-throughput neutralization assay for antibody detection.Results:Vaccination induced a substantial increase in neutralizing antibody geometric mean titers(GMT)post-immunization(4,808 after the first dose;5,326 after the second dose),with antibody levels remaining elevated in 4-year-old children(GMT:3,834).Crossneutralization activity against different genotypes varied by less than 6.4-fold,demonstrating broad protective immunity.However,12%of adult sera tested were seronegative,revealing the presence of susceptible populations.Conclusions:This study confirms the robust immunogenicity of the current measles vaccine and establishes a valuable tool for serosurveillance and longterm immunity assessment.展开更多
基金Supported by the Major Project of Guangzhou National Laboratory,Grant No.GZNL2024A01019.
文摘Introduction:This study aimed to establish a robust method for monitoring measles vaccine-induced immunity and assessing population-level serostatus.Methods:This study constructed a vesicular stomatitis virus(VSV)-based pseudotyped virus system expressing envelope proteins from seven major circulating measles genotypes(H1,B3,D4,D8,D9,D11,G3)and the Schwarz vaccine strain(genotype A),thereby enabling a high-throughput neutralization assay for antibody detection.Results:Vaccination induced a substantial increase in neutralizing antibody geometric mean titers(GMT)post-immunization(4,808 after the first dose;5,326 after the second dose),with antibody levels remaining elevated in 4-year-old children(GMT:3,834).Crossneutralization activity against different genotypes varied by less than 6.4-fold,demonstrating broad protective immunity.However,12%of adult sera tested were seronegative,revealing the presence of susceptible populations.Conclusions:This study confirms the robust immunogenicity of the current measles vaccine and establishes a valuable tool for serosurveillance and longterm immunity assessment.
