Since stem cell therapy is the most effective treatment in the field of tissue reparation and reconstitution,the present study aimed to explore the different sources of mesenchymal stem cells(MSCs)on the different eff...Since stem cell therapy is the most effective treatment in the field of tissue reparation and reconstitution,the present study aimed to explore the different sources of mesenchymal stem cells(MSCs)on the different effects of pulmonary fibrosis-related cytokines in C57BL/6 mice.For reaching this goal,we isolated MSCs from umbilical cord blood and placenta and used for stem cell therapy in a mouse model of pulmonary fibrosis model.The pulmonary fibrosis model was done by injecting bleomycin into the trachea of C57BL/6 mice.Then we assessed the degree of pulmonary fibrosis in each mouse lung tissue at weeks 1,2,3,and 4.In addition,flow cytometry was used to evaluate the frequency of CD73,CD90,CD106,CD34,CD45,CD14 cells at the mononuclear cell level;and western blotting assays revealed the expression of IκB-α.Our results showed that stem cell therapy by placenta-derived MSC had a lower level of CD34,CD45,CD14 cells at the mononuclear cell level,and that improved pulmonary fibrosis at both molecular and pathological levels.In addition,western blotting assays revealed that the expression of IκB-αwas down-regulated in MSC-treated animals.In addition,placenta-derived MSC was the most effective in improving pulmonary fibrosis in comparison to other sources.This study suggests that MSC might be a novel therapeutic approach in pulmonary fibrosis due to an enhanced anti-inflammatory effect.Also,MSC modification by gene editing could enhance their therapeutic effect in mouse pulmonary fibrosis.展开更多
Kiwifruit is an economically and nutritionally important horticultural fruit crop worldwide.The genomic data of several kiwifruit species have been released,providing an unprecedented opportunity for pan-genome analys...Kiwifruit is an economically and nutritionally important horticultural fruit crop worldwide.The genomic data of several kiwifruit species have been released,providing an unprecedented opportunity for pan-genome analysis to comprehensively investigate the inter-and intra-species genetic diversity and facilitate utilization for kiwifruit breeding.Here,we generated a kiwifruit super pan-genome using 15 high-quality assemblies of eight Actinidia species.For genebased pan-genome,a total of 61,465 gene families were identified,and the softcore and dispensable genes were enriched in biological processes like response to endogenous stimulus,response to hormone and cell wall organization or biogenesis.Then,structural variations(SVs)against A.chinensis‘Donghong’were identified and then used to construct a graph-based genome.Further population-scale SVs based on resequencing data from 112 individuals of 20 species revealed extensive SVs which probably contributed to the phenotypic diversity among the Actinidia species.SV hotspot regions were found contributed to environmental adaptation.Furthermore,we systematically identified resistance gene analogs(RGAs)in the 15 assemblies and generated a pan-RGA dataset to reveal the diversity of genes potentially involved in disease resistance in Actinidia.The pan-genomic data obtained here is useful for evolutionary and functional genomic studies in Actinidia,and facilitates breeding design.展开更多
In this study, based on the classical Archard adhesion wear theory, a three-dimensional finite element model was established, with the aim of simulating the failure process of self-lubricating spherical plain bearings...In this study, based on the classical Archard adhesion wear theory, a three-dimensional finite element model was established, with the aim of simulating the failure process of self-lubricating spherical plain bearings in the swinging wear condition. The results show that the self-lubricating spherical plain bearings go through two different stages during the wear process, namely, initial wear stage and stable wear stage. Because the large contact points wear out during the initial wear stage, the maximum contact pressure decreases as the test period increases. The relatively larger wear depth region shows elliptical distribution, and the maximum distribution appears in the central contact area. The wear depth reaches 0.974 mm after swinging 25,000 times. PTFE fibers, which possess a good friction performance but poor abrasion resistance, abundantly exist on the friction surfaces of the fabric liner. Consequently, the friction torque during the initial wear stage is slightly smaller than the friction torque during the stable wear stage; however, the wear rate during the initial wear stage is high. The reliability and effectiveness of the finite element model are verified by experiment. The developed finite element model can be used for the analysis of the wear mechanisms of bearings and the prediction of the service life of bearings.展开更多
Erratum to Friction 10(6):803–841(2022)Yahong Xue,Jigang Chen,Sumin Guo,Qingliang Meng&Junting Luo.Finite element simulation and experimental test of the wear behavior for self-lubricating spherical plain bearing...Erratum to Friction 10(6):803–841(2022)Yahong Xue,Jigang Chen,Sumin Guo,Qingliang Meng&Junting Luo.Finite element simulation and experimental test of the wear behavior for self-lubricating spherical plain bearings.Friction 6(3):297–306(2018).https://doi.org/10.1007/s40544-018-0206-x The authors regret that there was an inadvertent error in Fig.10(c)after checking the paper.It is important to note that the change does not impact the main conclusion of the article.Figure 10(c)on page 304 was selected as the incorrect picture,and the accurate version of Fig.10(c)is provided as below.展开更多
基金This work was financially supported by the National Natural Science Foundation of China(31001638&30870602)This project was supported by the key medical research plan of the Hebei province of China(No.ZD2013063)+1 种基金Tsing-hua University(2009THZ02122)National Heart,Lung,and Blood Institute Research Grant HL 104402 from the National Institutes of Health of the US Public Health Service.
文摘Since stem cell therapy is the most effective treatment in the field of tissue reparation and reconstitution,the present study aimed to explore the different sources of mesenchymal stem cells(MSCs)on the different effects of pulmonary fibrosis-related cytokines in C57BL/6 mice.For reaching this goal,we isolated MSCs from umbilical cord blood and placenta and used for stem cell therapy in a mouse model of pulmonary fibrosis model.The pulmonary fibrosis model was done by injecting bleomycin into the trachea of C57BL/6 mice.Then we assessed the degree of pulmonary fibrosis in each mouse lung tissue at weeks 1,2,3,and 4.In addition,flow cytometry was used to evaluate the frequency of CD73,CD90,CD106,CD34,CD45,CD14 cells at the mononuclear cell level;and western blotting assays revealed the expression of IκB-α.Our results showed that stem cell therapy by placenta-derived MSC had a lower level of CD34,CD45,CD14 cells at the mononuclear cell level,and that improved pulmonary fibrosis at both molecular and pathological levels.In addition,western blotting assays revealed that the expression of IκB-αwas down-regulated in MSC-treated animals.In addition,placenta-derived MSC was the most effective in improving pulmonary fibrosis in comparison to other sources.This study suggests that MSC might be a novel therapeutic approach in pulmonary fibrosis due to an enhanced anti-inflammatory effect.Also,MSC modification by gene editing could enhance their therapeutic effect in mouse pulmonary fibrosis.
基金supported by grants from the Hubei Provincial Natural Science Foundation of China(2024AFA035)the National Natural Science Foundation of China(32170395 and 32070377)the Foundation of Hubei Hongshan Laboratory(2021hszd017).
文摘Kiwifruit is an economically and nutritionally important horticultural fruit crop worldwide.The genomic data of several kiwifruit species have been released,providing an unprecedented opportunity for pan-genome analysis to comprehensively investigate the inter-and intra-species genetic diversity and facilitate utilization for kiwifruit breeding.Here,we generated a kiwifruit super pan-genome using 15 high-quality assemblies of eight Actinidia species.For genebased pan-genome,a total of 61,465 gene families were identified,and the softcore and dispensable genes were enriched in biological processes like response to endogenous stimulus,response to hormone and cell wall organization or biogenesis.Then,structural variations(SVs)against A.chinensis‘Donghong’were identified and then used to construct a graph-based genome.Further population-scale SVs based on resequencing data from 112 individuals of 20 species revealed extensive SVs which probably contributed to the phenotypic diversity among the Actinidia species.SV hotspot regions were found contributed to environmental adaptation.Furthermore,we systematically identified resistance gene analogs(RGAs)in the 15 assemblies and generated a pan-RGA dataset to reveal the diversity of genes potentially involved in disease resistance in Actinidia.The pan-genomic data obtained here is useful for evolutionary and functional genomic studies in Actinidia,and facilitates breeding design.
文摘In this study, based on the classical Archard adhesion wear theory, a three-dimensional finite element model was established, with the aim of simulating the failure process of self-lubricating spherical plain bearings in the swinging wear condition. The results show that the self-lubricating spherical plain bearings go through two different stages during the wear process, namely, initial wear stage and stable wear stage. Because the large contact points wear out during the initial wear stage, the maximum contact pressure decreases as the test period increases. The relatively larger wear depth region shows elliptical distribution, and the maximum distribution appears in the central contact area. The wear depth reaches 0.974 mm after swinging 25,000 times. PTFE fibers, which possess a good friction performance but poor abrasion resistance, abundantly exist on the friction surfaces of the fabric liner. Consequently, the friction torque during the initial wear stage is slightly smaller than the friction torque during the stable wear stage; however, the wear rate during the initial wear stage is high. The reliability and effectiveness of the finite element model are verified by experiment. The developed finite element model can be used for the analysis of the wear mechanisms of bearings and the prediction of the service life of bearings.
文摘Erratum to Friction 10(6):803–841(2022)Yahong Xue,Jigang Chen,Sumin Guo,Qingliang Meng&Junting Luo.Finite element simulation and experimental test of the wear behavior for self-lubricating spherical plain bearings.Friction 6(3):297–306(2018).https://doi.org/10.1007/s40544-018-0206-x The authors regret that there was an inadvertent error in Fig.10(c)after checking the paper.It is important to note that the change does not impact the main conclusion of the article.Figure 10(c)on page 304 was selected as the incorrect picture,and the accurate version of Fig.10(c)is provided as below.
