The AF9(protein AF9)transcription factor,encoded by MLLT3(mixed-lineage leukemia translocated to 3)on chromosome 9,functions as a chromatin reader.Through its N-terminal YEATS(Yaf9,ENL,AF9,Taf14,and Sas5)protein domai...The AF9(protein AF9)transcription factor,encoded by MLLT3(mixed-lineage leukemia translocated to 3)on chromosome 9,functions as a chromatin reader.Through its N-terminal YEATS(Yaf9,ENL,AF9,Taf14,and Sas5)protein domain,it interacts with acetylated[1]or crotonylated[2]histone H3,as well as with the PAF1(RNA polymerase II-associated factor 1 homolog)and P-TEFb(positive transcription elongation factor b)components of the super elongation complex(SEC).AF9 also interacts through its poly-serine domain(Poly-Ser)with the TFIID(Transcription factor II D)subunit of the RNA polymerase II(RNApol II)complex.In addition,its C-terminal transactivation domain,AHD(nuclear anchorage protein1 homology domain),binds other SEC components,such as AFF1 and AFF4(ALF transcription elongation factor 1 or 4),aswell as transcription regulators CBX8(chromobox 8),DOT1L(disruptor of telomeric silencing 1 like),and BCOR(B cell lymphoma 6 corepressor),as reviewed by Kabra&Bushweller[3](Figure 1A).Thus,MLLT3 is an integral part of the SEC,which is essential for optimizing the catalytic activity of RNApol II transcription at specific genome loci.展开更多
基金funded by INSERM,Institut Universitaire du Cancer-Toulouse(IUCT),Labex Toucan,Fondation Leucémie Espoir,Ligue Régionale Contre le Cancer,Fondation ARC,Association Laurette Fugain,Agence Nationale de la Recherche(ANR-18-CE45-0020 Transipedia)and(ANR-22-CE45-0007 full-RNA)ChloéBessière was supported by Fondation de France,Ahmed Zamani and Raissa Silva Da Silva by Ligue Nationale Contre le Cancer,Romain Pfeifer by Ministère de l’Enseignement Supérieur et de la Recherche and Fondation ARC,Sandr Dailhau by Ministère de la Santéand Institut National du Cancer(INCA,PRT-K-2022-184CircOma).
文摘The AF9(protein AF9)transcription factor,encoded by MLLT3(mixed-lineage leukemia translocated to 3)on chromosome 9,functions as a chromatin reader.Through its N-terminal YEATS(Yaf9,ENL,AF9,Taf14,and Sas5)protein domain,it interacts with acetylated[1]or crotonylated[2]histone H3,as well as with the PAF1(RNA polymerase II-associated factor 1 homolog)and P-TEFb(positive transcription elongation factor b)components of the super elongation complex(SEC).AF9 also interacts through its poly-serine domain(Poly-Ser)with the TFIID(Transcription factor II D)subunit of the RNA polymerase II(RNApol II)complex.In addition,its C-terminal transactivation domain,AHD(nuclear anchorage protein1 homology domain),binds other SEC components,such as AFF1 and AFF4(ALF transcription elongation factor 1 or 4),aswell as transcription regulators CBX8(chromobox 8),DOT1L(disruptor of telomeric silencing 1 like),and BCOR(B cell lymphoma 6 corepressor),as reviewed by Kabra&Bushweller[3](Figure 1A).Thus,MLLT3 is an integral part of the SEC,which is essential for optimizing the catalytic activity of RNApol II transcription at specific genome loci.
