Guided bone regeneration(GBR)uses resorbable and non-resorbable membranes as biological barriers.This study compared the differences in hard tissue stability between GBR using evidence-based digital titanium mesh and ...Guided bone regeneration(GBR)uses resorbable and non-resorbable membranes as biological barriers.This study compared the differences in hard tissue stability between GBR using evidence-based digital titanium mesh and resorbable collagen membranes during implant placement.A total of 40 patients(65 implant sites)were enrolled and divided into two groups:resorbable membrane and digital titanium mesh groups.The alveolar bone was analyzed at two-and three-dimensional levels using conebeam computed tomography and by reconstructing and superimposing the hard tissues at four time points:preoperatively,postoperatively,before second-stage surgery,and 1 year after loading.The use of digital titanium mesh showed less alveolar bone resorption in vertical and horizontal directions two-dimensionally before the second-stage surgery and 1 year after loading.Regarding volumetric stability,the percentage of resorption after 6 months of healing with resorbable membrane coverage reached 37.5%.However,it was only 23.4%with titanium mesh.Although postoperative bone volume was greater at all labial sites with resorbable membrane than with digital titanium mesh,after substantial bone resorption within 1 year of loading,the labial bone thickness at the upper part of implants was thinner with resorbable membrane than with digital titanium mesh.Furthermore,digital titanium meshes made according to ideal bone arch contour reduced soft tissue irritation,and the exposure rate was only 10%.Therefore,although both resorbable membrane and digital titanium mesh in GBR were able to successfully reconstruct the bone defect,digital titanium meshes were better at maintaining the hard tissue volume in the osteogenic space.展开更多
Guided bone regeneration(GBR)is an effective and simple method for bone augmentation,which is often used to reconstruct the alveolar ridge when the bone defect occurs in the implant area.Titanium mesh has expanded the...Guided bone regeneration(GBR)is an effective and simple method for bone augmentation,which is often used to reconstruct the alveolar ridge when the bone defect occurs in the implant area.Titanium mesh has expanded the indications of GBR technology due to its excellent mechanical properties and biocompatibility,so that the GBR technology can be used to repair alveolar ridges with larger bone defects,and can obtain excellent and stable bone augmentation results.Currently,GBR with titanium mesh has various clinical applications,including different clinical procedures.Bone graft materials,titanium mesh covering methods,and titanium mesh fixing methods are also optional.Moreover,the research of GBR with titanium mesh has led to multifarious progresses in digitalization and material modification.This article reviews the properties of titanium mesh and the difference of titanium mesh with other barrier membranes;the current clinical application of titanium mesh in bone augmentation;common complications and management and prevention methods in the application of titanium mesh;and research progress of titanium mesh in digitization and material modification.Hoping to provide a reference for further improvement of titanium mesh in clinical application and related research of titanium mesh.展开更多
Osteoarthritis, a disorder characterized by articular cartilage deterioration, varying degrees of inflammation, and chondrocyte apoptosis, is the most common chronic joint disease. To slow or reverse its progression, ...Osteoarthritis, a disorder characterized by articular cartilage deterioration, varying degrees of inflammation, and chondrocyte apoptosis, is the most common chronic joint disease. To slow or reverse its progression, inflammation should be inhibited, and chondrocyte proliferation should be promoted. Tetrahedral framework nucleic acids can be internalized by chondrocytes(even inflammatory chondrocytes) and can enhance their proliferation and migration. Wogonin, a naturally occurring flavonoid,suppresses oxidative stress and inhibits inflammation. In this study, tetrahedral framework nucleic acids were successfully selfassembled and used to load wogonin. We confirmed the effective formation of tetrahedral framework nucleic acid/wogonin complexes by dynamic light scattering, zeta potential analysis, transmission electron microscopy, and fluorescence spectrophotometry. Tetrahedral framework nucleic acids, wogonin, and especially tetrahedral framework nucleic acid/wogonin complexes effectively alleviated inflammation in vitro and in vivo and prevented cartilage destruction. In addition, these materials remarkably downregulated the expression of inflammatory mediators and matrix metalloproteinases, upregulated chondrogenic markers, and promoted tissue inhibitor of metalloproteinase 1 and B-cell lymphoma 2 expression. In vivo, after treatment with tetrahedral framework nucleic acid/wogonin complexes, the bone mineral density in regenerated tissues was much higher than that found in the untreated groups. Histologically, the complexes enhanced new tissue regeneration, significantly suppressed chondrocyte apoptosis, and promoted chondrogenic marker expression. They also inhibited cell apoptosis, increased chondrogenic marker expression, and suppressed the expression of inflammatory mediators in osteoarthritis. Therefore, we believe that tetrahedral framework nucleic acid/wogonin complexes can be used as an injectable form of therapy for osteoarthritis.展开更多
This study aimed to introduce a minimally invasive technique for maxillary sinus floor elevation using the lateral approach(lSFE)and to determine the factors that influence the stability of the grafted area in the sin...This study aimed to introduce a minimally invasive technique for maxillary sinus floor elevation using the lateral approach(lSFE)and to determine the factors that influence the stability of the grafted area in the sinus cavity.Thirty patients(30 implants)treated with lSFE using minimally invasive techniques from 2015 to 2019 were included in the study.Five aspects of the implant(central,mesial,distal,buccal,and palatal bone heights[BHs])were measured using cone-beam computed tomography(CBCT)before implant surgery,immediately after surgery(T0),6 months after surgery(T1),and at the last follow-up visit(T2).Patients’characteristics were collected.A small bone window(height,(4.40±0.74)mm;length,(6.26±1.03)mm)was prepared.No implant failed during the follow-up period(3.67±1.75)years.Three of the 30 implants exhibited perforations.Changes in BH of the five aspects of implants showed strong correlations with each other and BH decreased dramatically before second-stage surgery.Residual bone height(RBH)did not significantly influence BH changes,whereas smoking status and type of bone graft materials were the potentially influential factors.During the approximate three-year observation period,lSFE with a minimally invasive technique demonstrated high implant survival rate and limited bone reduction in grafted area.In conclusion,lSFE using minimally invasive techniques was a viable treatment option.Patients who were nonsmokers and whose sinus cavity was filled with deproteinized bovine bone mineral(DBBM)had significantly limited bone resorption in grafted area.展开更多
Synthetic antigen-encoding mRNA plays an increasingly significant role in tumor vaccine technology owing to its antigen-specific immune-activation. However, its immune efficacy is challenged by inferior delivery effic...Synthetic antigen-encoding mRNA plays an increasingly significant role in tumor vaccine technology owing to its antigen-specific immune-activation. However, its immune efficacy is challenged by inferior delivery efficiency and demand for suitable adjuvants. Here, we develop a novel mRNA nanovaccine based on a multifunctional nanocapsule, which is a dual-adjuvant formulation composed of cytosine-phosphateguanine motifs loaded tetrahedral framework nucleic acid(CpG-tFNA) and an immunopeptide murine β-defensin 2(mDF2β). This m RNA nanovaccine successfully achieves intracellular delivery, antigen expression and presentation of dendritic cells, and proliferation of antigen-specific T cells. In a tumor prophylactic vaccination model, it exerts an excellent inhibitory effect on lymphoma occurrence through cellular immunity. This mRNA nanovaccine has promising prophylactic applications in tumors and many other diseases.展开更多
Systemic immune monitoring is a crucial clinical tool for disease early diagnosis,prognosis and treatment planning by quantitative analysis of immune cells.However,conventional immune monitoring using flow cytometry f...Systemic immune monitoring is a crucial clinical tool for disease early diagnosis,prognosis and treatment planning by quantitative analysis of immune cells.However,conventional immune monitoring using flow cytometry faces huge challenges in large-scale sample testing,especially in mass health screenings,because of time-consuming,technical-sensitive and high-cost features.However,the lack of high-performance detection platforms hinders the development of high-throughput immune monitoring technology.To address this bottleneck,we constructed a generally applicable DNA framework signal amplification platform(DSAP)based on post-systematic evolution of ligands by exponential enrichment and DNA tetrahedral framework-structured probe design to achieve high-sensitive detection for diverse immune cells,including CD4+,CD8+T-lymphocytes,and monocytes(down to 1/100μl).Based on this advanced detection platform,we present a novel high-throughput immune-cell phenotyping system,DSAP,achieving 30-min one-step immune-cell phenotyping without cell washing and subset analysis and showing comparable accuracy with flow cytometry while significantly reducing detection time and cost.As a proof-of-concept,DSAP demonstrates excellent diagnostic accuracy in immunodeficiency staging for 107 HIV patients(AUC>0.97)within 30 min,which can be applied in HIV infection monitoring and screening.Therefore,we initially introduced promising DSAP to achieve high-throughput immune monitoring and open robust routes for point-of-care device development.展开更多
Periodontitis is a common disease that causes periodontium defects and tooth loss.Controlling inflammation and tissue regeneration are two key strategies in the treatment of periodontitis.Tetrahedral framework nucleic...Periodontitis is a common disease that causes periodontium defects and tooth loss.Controlling inflammation and tissue regeneration are two key strategies in the treatment of periodontitis.Tetrahedral framework nucleic acids can modulate multiple biological behaviors,and thus,their biological applications have been widely explored.In this study,we investigated the effect of tFNAs on periodontium under inflammatory conditions.Lipopolysaccharide and silk ligature were used to induce inflammation in vivo and in vitro.The results displayed that tFNAs decreased the release of pro-inflammatory cytokines and levels of cellular reactive oxygen species in periodontal ligament stem cells,which promoted osteogenic differentiation.Furthermore,animal experiments showed that tFNAs ameliorated the inflammation of the periodontium and protect periodontal tissue,especially reducing alveolar bone absorption by decreasing inflammatory infiltration and inhibiting osteoclast formation.These findings suggest that tFNAs can significantly improve the therapeutic effect of periodontitis and have the great potential significance in the field of periodontal tissue regeneration.展开更多
基金This study was supported by National Key R&D Program of China(2019YFA0110600)National Natural Science Foundation of China(81970986,81771125)Exploration and R&D Project of West China Hospital of Stomatology(LCYJ2020-DC-1).
文摘Guided bone regeneration(GBR)uses resorbable and non-resorbable membranes as biological barriers.This study compared the differences in hard tissue stability between GBR using evidence-based digital titanium mesh and resorbable collagen membranes during implant placement.A total of 40 patients(65 implant sites)were enrolled and divided into two groups:resorbable membrane and digital titanium mesh groups.The alveolar bone was analyzed at two-and three-dimensional levels using conebeam computed tomography and by reconstructing and superimposing the hard tissues at four time points:preoperatively,postoperatively,before second-stage surgery,and 1 year after loading.The use of digital titanium mesh showed less alveolar bone resorption in vertical and horizontal directions two-dimensionally before the second-stage surgery and 1 year after loading.Regarding volumetric stability,the percentage of resorption after 6 months of healing with resorbable membrane coverage reached 37.5%.However,it was only 23.4%with titanium mesh.Although postoperative bone volume was greater at all labial sites with resorbable membrane than with digital titanium mesh,after substantial bone resorption within 1 year of loading,the labial bone thickness at the upper part of implants was thinner with resorbable membrane than with digital titanium mesh.Furthermore,digital titanium meshes made according to ideal bone arch contour reduced soft tissue irritation,and the exposure rate was only 10%.Therefore,although both resorbable membrane and digital titanium mesh in GBR were able to successfully reconstruct the bone defect,digital titanium meshes were better at maintaining the hard tissue volume in the osteogenic space.
基金This study was supported by National Natural Science Foundation of China(Grant Nos.81970986,81771125,X.C.and 11872135,12072055 C.W.)。
文摘Guided bone regeneration(GBR)is an effective and simple method for bone augmentation,which is often used to reconstruct the alveolar ridge when the bone defect occurs in the implant area.Titanium mesh has expanded the indications of GBR technology due to its excellent mechanical properties and biocompatibility,so that the GBR technology can be used to repair alveolar ridges with larger bone defects,and can obtain excellent and stable bone augmentation results.Currently,GBR with titanium mesh has various clinical applications,including different clinical procedures.Bone graft materials,titanium mesh covering methods,and titanium mesh fixing methods are also optional.Moreover,the research of GBR with titanium mesh has led to multifarious progresses in digitalization and material modification.This article reviews the properties of titanium mesh and the difference of titanium mesh with other barrier membranes;the current clinical application of titanium mesh in bone augmentation;common complications and management and prevention methods in the application of titanium mesh;and research progress of titanium mesh in digitization and material modification.Hoping to provide a reference for further improvement of titanium mesh in clinical application and related research of titanium mesh.
基金supported by National Key R&D Program of China (2019YFA0110600)National Natural Science Foundation of China (81970986, 81771125)
文摘Osteoarthritis, a disorder characterized by articular cartilage deterioration, varying degrees of inflammation, and chondrocyte apoptosis, is the most common chronic joint disease. To slow or reverse its progression, inflammation should be inhibited, and chondrocyte proliferation should be promoted. Tetrahedral framework nucleic acids can be internalized by chondrocytes(even inflammatory chondrocytes) and can enhance their proliferation and migration. Wogonin, a naturally occurring flavonoid,suppresses oxidative stress and inhibits inflammation. In this study, tetrahedral framework nucleic acids were successfully selfassembled and used to load wogonin. We confirmed the effective formation of tetrahedral framework nucleic acid/wogonin complexes by dynamic light scattering, zeta potential analysis, transmission electron microscopy, and fluorescence spectrophotometry. Tetrahedral framework nucleic acids, wogonin, and especially tetrahedral framework nucleic acid/wogonin complexes effectively alleviated inflammation in vitro and in vivo and prevented cartilage destruction. In addition, these materials remarkably downregulated the expression of inflammatory mediators and matrix metalloproteinases, upregulated chondrogenic markers, and promoted tissue inhibitor of metalloproteinase 1 and B-cell lymphoma 2 expression. In vivo, after treatment with tetrahedral framework nucleic acid/wogonin complexes, the bone mineral density in regenerated tissues was much higher than that found in the untreated groups. Histologically, the complexes enhanced new tissue regeneration, significantly suppressed chondrocyte apoptosis, and promoted chondrogenic marker expression. They also inhibited cell apoptosis, increased chondrogenic marker expression, and suppressed the expression of inflammatory mediators in osteoarthritis. Therefore, we believe that tetrahedral framework nucleic acid/wogonin complexes can be used as an injectable form of therapy for osteoarthritis.
基金supported by National Natural Science Foundation of China(81970986,81771125)Exploration and R&D Project of West China Hospital of Stomatology(LCYJ2020-DC-1)+1 种基金Sichuan University Postdoctoral Innovation Fund(2023SCU12040)China Postdoctoral Science Foundation funded project(2022TQ0222).
文摘This study aimed to introduce a minimally invasive technique for maxillary sinus floor elevation using the lateral approach(lSFE)and to determine the factors that influence the stability of the grafted area in the sinus cavity.Thirty patients(30 implants)treated with lSFE using minimally invasive techniques from 2015 to 2019 were included in the study.Five aspects of the implant(central,mesial,distal,buccal,and palatal bone heights[BHs])were measured using cone-beam computed tomography(CBCT)before implant surgery,immediately after surgery(T0),6 months after surgery(T1),and at the last follow-up visit(T2).Patients’characteristics were collected.A small bone window(height,(4.40±0.74)mm;length,(6.26±1.03)mm)was prepared.No implant failed during the follow-up period(3.67±1.75)years.Three of the 30 implants exhibited perforations.Changes in BH of the five aspects of implants showed strong correlations with each other and BH decreased dramatically before second-stage surgery.Residual bone height(RBH)did not significantly influence BH changes,whereas smoking status and type of bone graft materials were the potentially influential factors.During the approximate three-year observation period,lSFE with a minimally invasive technique demonstrated high implant survival rate and limited bone reduction in grafted area.In conclusion,lSFE using minimally invasive techniques was a viable treatment option.Patients who were nonsmokers and whose sinus cavity was filled with deproteinized bovine bone mineral(DBBM)had significantly limited bone resorption in grafted area.
基金supported by National Key R&D Program of China (No. 2019YFA0110600)National Natural Science Foundation of China (No. 81970916)+2 种基金Sichuan Province Youth Science and Technology Innovation Team (No. 2022JDTD0021)Research Funding from West China School/Hospital of Stomatology Sichuan University (No. RCDWJS2021-20)China Postdoctoral Science Foundation (No. 2022TQ0381)。
文摘Synthetic antigen-encoding mRNA plays an increasingly significant role in tumor vaccine technology owing to its antigen-specific immune-activation. However, its immune efficacy is challenged by inferior delivery efficiency and demand for suitable adjuvants. Here, we develop a novel mRNA nanovaccine based on a multifunctional nanocapsule, which is a dual-adjuvant formulation composed of cytosine-phosphateguanine motifs loaded tetrahedral framework nucleic acid(CpG-tFNA) and an immunopeptide murine β-defensin 2(mDF2β). This m RNA nanovaccine successfully achieves intracellular delivery, antigen expression and presentation of dendritic cells, and proliferation of antigen-specific T cells. In a tumor prophylactic vaccination model, it exerts an excellent inhibitory effect on lymphoma occurrence through cellular immunity. This mRNA nanovaccine has promising prophylactic applications in tumors and many other diseases.
基金supported by the National Key R&D Program of China(2019YFA0110600)National Natural Science Foundation of China(82370929,81970916,81671031,82201027 and 81800947)+3 种基金Postdoctoral Science Foundation of China(2021M70233 and 2021TQ0224)Sichuan Science and Technology Program(2022NSFSC0002,2022JDRC0143 and 2022ZYD0074)Research Funding was also obtained from West China School/Hospital of Stomatology Sichuan University(RD03202302,RCDWJS2022-12)Sichuan Province Youth Science and Technology Innovation Team(2022JDTD0021).
文摘Systemic immune monitoring is a crucial clinical tool for disease early diagnosis,prognosis and treatment planning by quantitative analysis of immune cells.However,conventional immune monitoring using flow cytometry faces huge challenges in large-scale sample testing,especially in mass health screenings,because of time-consuming,technical-sensitive and high-cost features.However,the lack of high-performance detection platforms hinders the development of high-throughput immune monitoring technology.To address this bottleneck,we constructed a generally applicable DNA framework signal amplification platform(DSAP)based on post-systematic evolution of ligands by exponential enrichment and DNA tetrahedral framework-structured probe design to achieve high-sensitive detection for diverse immune cells,including CD4+,CD8+T-lymphocytes,and monocytes(down to 1/100μl).Based on this advanced detection platform,we present a novel high-throughput immune-cell phenotyping system,DSAP,achieving 30-min one-step immune-cell phenotyping without cell washing and subset analysis and showing comparable accuracy with flow cytometry while significantly reducing detection time and cost.As a proof-of-concept,DSAP demonstrates excellent diagnostic accuracy in immunodeficiency staging for 107 HIV patients(AUC>0.97)within 30 min,which can be applied in HIV infection monitoring and screening.Therefore,we initially introduced promising DSAP to achieve high-throughput immune monitoring and open robust routes for point-of-care device development.
基金This study was supported by National Key R&D Program of China(2019YFA0110600)National Natural Science Foundation of China(81970986,81771125).
文摘Periodontitis is a common disease that causes periodontium defects and tooth loss.Controlling inflammation and tissue regeneration are two key strategies in the treatment of periodontitis.Tetrahedral framework nucleic acids can modulate multiple biological behaviors,and thus,their biological applications have been widely explored.In this study,we investigated the effect of tFNAs on periodontium under inflammatory conditions.Lipopolysaccharide and silk ligature were used to induce inflammation in vivo and in vitro.The results displayed that tFNAs decreased the release of pro-inflammatory cytokines and levels of cellular reactive oxygen species in periodontal ligament stem cells,which promoted osteogenic differentiation.Furthermore,animal experiments showed that tFNAs ameliorated the inflammation of the periodontium and protect periodontal tissue,especially reducing alveolar bone absorption by decreasing inflammatory infiltration and inhibiting osteoclast formation.These findings suggest that tFNAs can significantly improve the therapeutic effect of periodontitis and have the great potential significance in the field of periodontal tissue regeneration.
