OBJECTIVE:To investigate the pathway through which Calculus Bovis Sativus (CBS) up-regulates hepatic multidrug resistance-associated protein 2 (Mrp2) and Mrp4 in 17α-ethynylestradiol (EE)-induced cholestasis.METHODS:...OBJECTIVE:To investigate the pathway through which Calculus Bovis Sativus (CBS) up-regulates hepatic multidrug resistance-associated protein 2 (Mrp2) and Mrp4 in 17α-ethynylestradiol (EE)-induced cholestasis.METHODS:Five groups of rats were designed:control group,EE+ICI182780 group,EE group,EE+CBS 50 mg/kg group and EE + CBS 150 mg/kg group.CBS (50 and 150 mg.kg-1· d-1) was orally given to rats by gavage for five consecutive days in coadministration with EE.The levels of cholestasis biomarkers,alanine aminotransferase (ALT),aspartate aminotransferase (AST),alkaline phosphatase (ALP) and total bilirubin (TBIL) were determined by biochemical methods.The bile flow was measured.The histopathology of the liver tissue was evaluated.The expression of Mrp2,Mrp3,Mrp4,estrogen receptor α(ERα) and ERβ was determined by Western blotting.RESULTS:CBS markedly improved EE-induced cholestasis.EE exposure significantly reduced hepatic Mrp2 and Mrp4 expression compared with the control group.EE also dramatically up-regulated the expression of Mrp3.Compared to the EE group,CBS notably up-regulated hepatic Mrp2 and Mrp4 but failed to influence the Mrp3 level significantly.ICI182780,an ER antagonist,showed similar beneficial effects as CBS.Decreased expression of Mrp2 and Mrp4 caused by EE was also restored by IC1182780.Additionally,EE significantly induced hepatic ERα expression,which was reversed by ICI182780 or CBS (150 mg/kg) treatment,suggesting that CB5 exerted a moderate regulatory effect on ER signaling.CONCLUSION:CBS up-regulated hepatic Mrp2 and Mrp4 expression in EE-induced cholestasis,which might be associated with its regulation of ER signaling.展开更多
美国放射学会(American College of Radiology,ACR)编写的《乳腺影像报告和数据系统》(Breast Imaging Reporting and Data System,BI-RADS)[1]旨在规范乳腺影像检查的描述术语、报告格式、检查技术和诊断程序,以促进医生之间的沟通,并...美国放射学会(American College of Radiology,ACR)编写的《乳腺影像报告和数据系统》(Breast Imaging Reporting and Data System,BI-RADS)[1]旨在规范乳腺影像检查的描述术语、报告格式、检查技术和诊断程序,以促进医生之间的沟通,并指导临床实践,目前BI-RADS已被广泛应用于我国乳腺超声临床工作中。2003年第4版BI-RADS首次引入超声部分,2013年第5版BI-RADS在此基础上进一步完善。近十年来,无论是筛查还是诊断,超声在乳腺成像中的重要性均日益凸显,即将正式发布的第6版BI-RADS将基于最新的文献资料和专家共识,对先前版本进行修正和补充,力求提升乳腺成像方法之间表述的一致性。展开更多
基金Supported by the National Natural Science Foundation of China(Study of the Role and Mechanism of PDZK1 Protein in the Downregulation of Mrp2 by Estrogen in Cholestasis,No.81503146 and Study of the Choleretic Effect of Calculus Bovis Sativus Based on PDZK1 Protein Interaction Networks and Bile Acid Metabolic Profile,No.81573788)Science Foundations of Health and Family Planning Commission of Wuhan Municipality(No.WZ15Z02)
文摘OBJECTIVE:To investigate the pathway through which Calculus Bovis Sativus (CBS) up-regulates hepatic multidrug resistance-associated protein 2 (Mrp2) and Mrp4 in 17α-ethynylestradiol (EE)-induced cholestasis.METHODS:Five groups of rats were designed:control group,EE+ICI182780 group,EE group,EE+CBS 50 mg/kg group and EE + CBS 150 mg/kg group.CBS (50 and 150 mg.kg-1· d-1) was orally given to rats by gavage for five consecutive days in coadministration with EE.The levels of cholestasis biomarkers,alanine aminotransferase (ALT),aspartate aminotransferase (AST),alkaline phosphatase (ALP) and total bilirubin (TBIL) were determined by biochemical methods.The bile flow was measured.The histopathology of the liver tissue was evaluated.The expression of Mrp2,Mrp3,Mrp4,estrogen receptor α(ERα) and ERβ was determined by Western blotting.RESULTS:CBS markedly improved EE-induced cholestasis.EE exposure significantly reduced hepatic Mrp2 and Mrp4 expression compared with the control group.EE also dramatically up-regulated the expression of Mrp3.Compared to the EE group,CBS notably up-regulated hepatic Mrp2 and Mrp4 but failed to influence the Mrp3 level significantly.ICI182780,an ER antagonist,showed similar beneficial effects as CBS.Decreased expression of Mrp2 and Mrp4 caused by EE was also restored by IC1182780.Additionally,EE significantly induced hepatic ERα expression,which was reversed by ICI182780 or CBS (150 mg/kg) treatment,suggesting that CB5 exerted a moderate regulatory effect on ER signaling.CONCLUSION:CBS up-regulated hepatic Mrp2 and Mrp4 expression in EE-induced cholestasis,which might be associated with its regulation of ER signaling.
文摘美国放射学会(American College of Radiology,ACR)编写的《乳腺影像报告和数据系统》(Breast Imaging Reporting and Data System,BI-RADS)[1]旨在规范乳腺影像检查的描述术语、报告格式、检查技术和诊断程序,以促进医生之间的沟通,并指导临床实践,目前BI-RADS已被广泛应用于我国乳腺超声临床工作中。2003年第4版BI-RADS首次引入超声部分,2013年第5版BI-RADS在此基础上进一步完善。近十年来,无论是筛查还是诊断,超声在乳腺成像中的重要性均日益凸显,即将正式发布的第6版BI-RADS将基于最新的文献资料和专家共识,对先前版本进行修正和补充,力求提升乳腺成像方法之间表述的一致性。
