Polyacrylic acid(PAA)-based binders have been demonstrated to significantly enhance the cycling stability of pure silicon(Si)anodes compared to other binder types.However,there is a notable lack of systematic and in-d...Polyacrylic acid(PAA)-based binders have been demonstrated to significantly enhance the cycling stability of pure silicon(Si)anodes compared to other binder types.However,there is a notable lack of systematic and in-depth investigation into the relationship between the molecular weight(MW)of PAA and its performance in pure Si anodes,leading to an absence of reliable theoretical guidance for designing and optimizing of PAA-based binders for these anodes.Herein,we select a series of PAA with varying MWs as binders for Si nanoparticle(SiNP)anodes to systematically identify the optimal MW of PAA for enhancing the electrochemical performance of SiNP anodes.The actual MWs of the various PAA were confirmed by gel permeation chromatography to accurately establish the relationship between MW and binder performance.Within an ultrawide weight average molecular weight(M_(w))range of 35.9-4850 kDa,we identify that the PAA binder with a M_(w)of 1250 kDa(PAA125)exhibits the strongest mechanical strength and the highest adhesion strength,attributed to its favorable molecular chain orientation and robust interchain interactions.These characteristics enable the SiNP anodes utilizing PAA125 to maintain the best interfacial chemistry and bulk mechanical structure stability,leading to optimal electrochemical performance.Notably,the enhancement in cycling stability of SiNP anode by PAA125 under practical application conditions is further validated by the 1.1 Ah LLNi_(0.8)Co_(0.1)Mn_(0.1)O_(2)/SiNP@PAA125 pouch cell.展开更多
In most kinds of animal cells, the centrosome serves as the main microtubule organizing center （MTOC） that nucleates microtubule arrays throughout the cytoplasm to maintain cell structure, cell division and intracel...In most kinds of animal cells, the centrosome serves as the main microtubule organizing center （MTOC） that nucleates microtubule arrays throughout the cytoplasm to maintain cell structure, cell division and intracellular transport. Whereas in epithelial cells, non-centrosomal MTOCs are established in the apical domain for generating asymmetric microtubule fibers and cilia in epithelial cells for the organ morphogenesis during embryonic development. However, the mechanism by which MTOCs localize to the apical domain in epithelial cells remains largely unknown. Here, we show that Mid liplb has a close interaction with γ-tubulin protein, the central component of MTOC, and modulates lumen opening of the neural tube, gut, intestine, and kidney of zebrafish. Knockdown or dominant negative effect of Mid 1ip 1 b resulted in failure of lumen formation of the organs as aforementioned. Moreover, the non-centrosomal MTOCs were unable to orientate to the apical domain in Midliplb knockdown epithelial cells, and the centrosomal MTOCs were inaccurately placed in the apical domain, resulting in defective formation of asymmetric microtubules and misplacement of cilia in the apical domain. These data uncover a molecule that controls the proper localization of MTOCs in the apical domain in epithelial cells for organ morphogenesis during embryonic development.展开更多
In worldwide,the use of chemical pesticides to protect wood has been greatly restricted.In recent years,a large number of researchers devoted to the search for natural,safe and non-polluting bioactive chemical compoun...In worldwide,the use of chemical pesticides to protect wood has been greatly restricted.In recent years,a large number of researchers devoted to the search for natural,safe and non-polluting bioactive chemical compounds from plants as an alternative to synthetic organic chemical preservative.In Chinese folk,tung oil can be used as paint for wooden furniture to protect them from pests.This study aimed to evaluate the chemical compositions of raw and heated tung oil and their activity against termite.In choice bioassays,weight loss of wood treated with 5%raw or heated tung oil after 4 weeks was significantly less than that of the control group.In no-choice bioassays,there was a significant difference in termite survival and wood weight loss on raw and heated tung oil-treated wood.When tung oil-treatment concentrations increased to 5%,wood weight loss was less than 10%.There was no significant difference in termite survival and wood weight loss between raw and heated tung oil-treated wood.Survival of termites in both tung oil wood treatments was significantly lower than that in the starvation control after 4 weeks.Raw and heated tung oil significantly improved the resistance of pine wood to termites,and have the potential for the development of natural wood preservatives.展开更多
Dementias such as Alzheimer disease(AD)and mild cognitive impairment(MCI)lead to problems with memory,language,and daily activities resulting from damage to neurons in the brain.Given the irreversibility of this neuro...Dementias such as Alzheimer disease(AD)and mild cognitive impairment(MCI)lead to problems with memory,language,and daily activities resulting from damage to neurons in the brain.Given the irreversibility of this neuronal damage,it is crucial to find a biomarker to distinguish individuals with these diseases from healthy people.In this study,we construct a brain function network based on electroencephalography data to study changes in AD and MCI patients.Using a graph-theoretical approach,we examine connectivity features and explore their contributions to dementia recognition at edge,node,and network levels.We find that connectivity is reduced in AD and MCI patients compared with healthy controls.We also find that the edge-level features give the best performance when machine learning models are used to recognize dementia.The results of feature selection identify the top 50 ranked edge-level features constituting an optimal subset,which is mainly connected with the frontal nodes.A threshold analysis reveals that the performance of edge-level features is more sensitive to the threshold for the connection strength than that of node-and network-level features.In addition,edge-level features with a threshold of 0 provide the most effective dementia recognition.The K-nearest neighbors(KNN)machine learning model achieves the highest accuracy of 0.978 with the optimal subset when the threshold is 0.Visualization of edge-level features suggests that there are more long connections linking the frontal region with the occipital and parietal regions in AD and MCI patients compared with healthy controls.Our codes are publicly available at https://github.com/Debbie-85/eeg-connectivity.展开更多
Fluorine substitution was applied to the donor and acceptor segments of block copolymers to understand the impact of molecular structure on photovoltaic block copolymers and explore efficient materials for single-comp...Fluorine substitution was applied to the donor and acceptor segments of block copolymers to understand the impact of molecular structure on photovoltaic block copolymers and explore efficient materials for single-component organic solar cells(SCOSCs).Along this line,three fluorinated block copolymers,namely PBDB-T-b-PTYF6,PM6-b-PTY6,and PM6-bPTYF6,derived from PBDB-T-b-PTY6 were designed and synthesized.The UV-Vis absorption,energy level,and thin-film morphology of these block copolymers were systematically characterized.All fluorinated block copolymers show narrow bandgap and improved crystallinity.An enhanced open-circuit voltage was observed in the SCOSC based on PM6-b-PTY6.However,SCOSCs based on all fluorinated block copolymers exhibited low short-circuit current due to energy level mismatch and therefore had low power conversion efficiency at around 4%.By contrast,the SCOSCs based on control block copolymer PBDB-T-b-PTY6 exhibited the highest power conversion efficiency approaching 10%,with a high short-circuit current of 18.57 mA/cm~2.Our study was the first to perform fluorination on photovoltaic block copolymers and provides insight into precisely controlling the polymer structure and understanding the structure-property relationship in SCOSCs based on block copolymers.展开更多
The main causes of floods in small and medium rivers in Jiangxi Province are meteorological factors,rainstorm factors and topographic factors.Based on the data of more than 600 floods from 19 hydrological stations in ...The main causes of floods in small and medium rivers in Jiangxi Province are meteorological factors,rainstorm factors and topographic factors.Based on the data of more than 600 floods from 19 hydrological stations in small and medium rivers in Jiangxi Province,the characteristics of floods in small and medium rivers are analyzed. The results show that the floods in small and medium rivers in Jiangxi Province had characteristics of strong seasonality,high frequency and strong suddenness,and were mainly local floods;rainstorm floods during May-June lasted for a long term,and had double peaks,multi-peaks or a single peak with a long duration. During July-September,floods usually had a single peak,and the duration was relatively short.展开更多
Background and purpose Medullary infarction(MI)caused by spontaneous vertebral artery dissection(sVAD)is an important type of stroke.It is important to distinguish sVAD from other causes of stroke since the treatment ...Background and purpose Medullary infarction(MI)caused by spontaneous vertebral artery dissection(sVAD)is an important type of stroke.It is important to distinguish sVAD from other causes of stroke since the treatment strategies and prognosis were different between them.In this study,we aimed to explore the clinical and radiological features of MI in patients with acute MI caused by sVAD.Methods Patients with acute MI caused by sVAD and non-sVAD in a single tertiary hospital were enrolled from 2010 to 2020.Epidemiologic,clinical and image features were collected and analysed.MI lesions were categorised into three levels rostrocaudally and four arterial groups:anteromedial,anterolateral,lateral and posterior.Results A total of 128 patients with MI were enrolled with 47 cases of sVAD and 81 cases of non-sVAD.Patients with sVAD were younger than those with non-sVAD(med 44 years old vs 58 years old).The sVAD group was less likely to have hypertension(44.68%vs 67.90%;p=0.010)and diabetes(19.15%vs 45.69%;p=0.003),but more likely to have non-sudden onset(27.66%vs 9.87%,p=0.009),minor neck injury(19.15%vs 1.23%;p=0.001)and headache(46.81%vs 7.41%;p=0.000).Vertically,sVAD became more common in caudal medulla than in rostral medulla.Horizontally,the sVAD group was more likely to have lateral MI(91.48%vs 2.96%,p=0.000).In multivariable logistic regression analysis,age,non-sudden onset and headache were independently associated with sVAD with ORs of 0.935(95%CI 0.892 to 0.981,p=0.006),3.507(95%CI 1.060 to 11.599,p=0.040)and 5.426(95%CI 1.673 to 17.599,p=0.005).Conclusion sVAD was not uncommon in patients with MI,especially in patients with lateral MI.Young patients with headache and non-sudden onset should remind clinician the possibility of sVAD.展开更多
Background:Liver ischemia/reperfusion(I/R)injury is a complex and multifactorial pathophysiological process.It is well recognized that the membrane permeability transition pore(mPTP)opening of mitochondria plays a cru...Background:Liver ischemia/reperfusion(I/R)injury is a complex and multifactorial pathophysiological process.It is well recognized that the membrane permeability transition pore(mPTP)opening of mitochondria plays a crucial role in cell death after I/R injury.Cyclophilin D(CypD)is a critical positive regulator of mPTP.However,the effect of CypD on the pathogenesis of liver I/R injury and whether CypD is a potential therapeutic target are still unclear.Methods:We constructed liver-specific CypD knockout and AAV8-peptidyl prolyl isomerase F(PPIF)overexpression mice.Then,a 70%liver I/R injury model was established in mice,with 90 min of ischemia and 6 h of reperfusion.The liver function was detected by the level of serum glutamic pyruvic transaminase(alanine transaminase)and glutamic oxaloacetic transaminase(aspartate aminotransferase),the liver damage score and degree of necrosis were measured by hematoxylin and eosin(H&E)staining of liver tissues.Reactive oxygen species(ROS)staining,apoptosis,and autophagy-related molecules were used to detect apoptosis and autophagy during liver I/R.Results:The liver-specific knockout of CypD alleviated necrosis and dysfunction in liver I/R injury,by reducing the excessive production of ROS,and inhibiting cell apoptosis and autophagy.On the contrary,overexpression of CypD exacerbated I/R-induced liver damage.Conclusion:We found that the downregulation of CypD expression alleviated liver I/R injury by reducing apoptosis and autophagy through caspase-3/Beclin1 crosstalk;in contrast,the upregulation of CypD expression aggravated liver I/R injury.Therefore,interfering with the expression of CypD seems to be a promising treatment for liver I/R injury.展开更多
Background:To compare the efficacy and safety of preoperative intravitreal injections of ranibizumab and conbercept in Chinese proliferative diabetic retinopathy(PDR)patients.Methods:This prospective randomized contro...Background:To compare the efficacy and safety of preoperative intravitreal injections of ranibizumab and conbercept in Chinese proliferative diabetic retinopathy(PDR)patients.Methods:This prospective randomized controlled trial enrolled 90 eyes of 80 patients with PDR.Forty-four eyes of 40 patients that received intravitreal ranibizumab(IVR)injections(0.5 mg/0.05 mL)before vitreous surgeries were assigned to the IVR group.Forty-six eyes of 40 patients that received intravitreal conbercept(IVC)injections(0.5 mg/0.05 mL)before vitreous surgeries were assigned to the IVC group.Intraoperative and postoperative indices were assessed for further comparison between the two groups.Results:There were no statistically significant differences in all surgery indices,including intraoperative indices(surgery time,P=0.225;intraoperative bleeding,P=0.808;endodiathermy use,P=0.693;incidence of iatrogenic retinal breaks,P=0.740;relaxing retinotomy,P=0.682;retinal reattachment,P=0.682 and silicone oil tamponade,P=0.814)and postoperative indices(postoperative vitreous hemorrhage(VH),P=0.808;neovascular glaucoma(NVG),P=0.964;recurrent retinal detachment,P=0.531;postoperative fibrovascular proliferation progression,P=0.682 and reoperation,P=0.955)between the two groups.There were no statistically significant differences in best-corrected visual acuity(BCVA)at each follow-up visit(P=0.939,0.669,0.741 and 0.717,respectively)or in central retinal thickness(CRT)(P=0.976,0.699,0.551 and 0.686,respectively).As for safety profile,both groups had no ocular or system adverse events during the observation period.Conclusions:IVR and IVC as a pretreatment of vitrectomy had similar efficacy and safety profile for Chinese PDR patients.展开更多
Single-atom site(SAS)catalysts have attracted considerable attention due to their excellent performance.However,most of the current research models of SAS catalysts are based on inorganic catalysts,where“metal and co...Single-atom site(SAS)catalysts have attracted considerable attention due to their excellent performance.However,most of the current research models of SAS catalysts are based on inorganic catalysts,where“metal and coordination atom interaction”cannot simulate the fine-tuning effect of organic ligands on metal catalytic centers in homogeneous catalysts.Therefore,certain chemical transformations in homogeneous catalysis cannot be perfectly replicated.Here,we used porous organic ligand polymers as the carrier,which effectively changes the charge regulation of nanoparticles and monoatomic metal catalysts.Drawing lessons from traditional homogeneous metal/ligand catalysis,we introduced various functional groups into the ligand polymers to adjust the electronic properties,and successfully realized the hydrosilylation of internal alkynes with high catalytic performance.The selectivity and catalytic efficiency under the Pd@POL-1 catalyst system were improved compared with previous studies.The internal alkynes with various structures can complete this reaction,and the ratio of E/Zcan reach up to 100:1.展开更多
Soft conductive films composed of a silver nanowire（AgNW） network, a neutral-pH PEDOT：PSS overcoating layer and a polydimethylsiloxane（PDMS） elastomer substrate are fabricated by large area compatible coating pro...Soft conductive films composed of a silver nanowire（AgNW） network, a neutral-pH PEDOT：PSS overcoating layer and a polydimethylsiloxane（PDMS） elastomer substrate are fabricated by large area compatible coating processes. The neutral-pH PEDOT：PSS layer is shown to be able to significantly improve the conductivity,stretchability and air stability of the conductive films. The soft conductive films are patterned using a simple maskless patterning approach to fabricate an 8 x 8 flexible pressure sensor array. It is shown that such soft conductive films can help to improve the sensitivity and reduce the signal crosstalk over the pressure sensor array.展开更多
基金funding supports of the National Natural Science Foundation of China(52402315,52172244,51874104,and 52172190)the"Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang"(2023R01007)the Zhejiang Provincial"Jianbing"and"Lingyan"R&D Programs(Grant No.2024C01262)。
文摘Polyacrylic acid(PAA)-based binders have been demonstrated to significantly enhance the cycling stability of pure silicon(Si)anodes compared to other binder types.However,there is a notable lack of systematic and in-depth investigation into the relationship between the molecular weight(MW)of PAA and its performance in pure Si anodes,leading to an absence of reliable theoretical guidance for designing and optimizing of PAA-based binders for these anodes.Herein,we select a series of PAA with varying MWs as binders for Si nanoparticle(SiNP)anodes to systematically identify the optimal MW of PAA for enhancing the electrochemical performance of SiNP anodes.The actual MWs of the various PAA were confirmed by gel permeation chromatography to accurately establish the relationship between MW and binder performance.Within an ultrawide weight average molecular weight(M_(w))range of 35.9-4850 kDa,we identify that the PAA binder with a M_(w)of 1250 kDa(PAA125)exhibits the strongest mechanical strength and the highest adhesion strength,attributed to its favorable molecular chain orientation and robust interchain interactions.These characteristics enable the SiNP anodes utilizing PAA125 to maintain the best interfacial chemistry and bulk mechanical structure stability,leading to optimal electrochemical performance.Notably,the enhancement in cycling stability of SiNP anode by PAA125 under practical application conditions is further validated by the 1.1 Ah LLNi_(0.8)Co_(0.1)Mn_(0.1)O_(2)/SiNP@PAA125 pouch cell.
基金supported by the National Basic Research Program of China (to X.M.,2015CB942800)the Nature Science Foundation of China (to H. X. 31671502)
文摘In most kinds of animal cells, the centrosome serves as the main microtubule organizing center （MTOC） that nucleates microtubule arrays throughout the cytoplasm to maintain cell structure, cell division and intracellular transport. Whereas in epithelial cells, non-centrosomal MTOCs are established in the apical domain for generating asymmetric microtubule fibers and cilia in epithelial cells for the organ morphogenesis during embryonic development. However, the mechanism by which MTOCs localize to the apical domain in epithelial cells remains largely unknown. Here, we show that Mid liplb has a close interaction with γ-tubulin protein, the central component of MTOC, and modulates lumen opening of the neural tube, gut, intestine, and kidney of zebrafish. Knockdown or dominant negative effect of Mid 1ip 1 b resulted in failure of lumen formation of the organs as aforementioned. Moreover, the non-centrosomal MTOCs were unable to orientate to the apical domain in Midliplb knockdown epithelial cells, and the centrosomal MTOCs were inaccurately placed in the apical domain, resulting in defective formation of asymmetric microtubules and misplacement of cilia in the apical domain. These data uncover a molecule that controls the proper localization of MTOCs in the apical domain in epithelial cells for organ morphogenesis during embryonic development.
基金This study was funded by College Students’Scientific Research Training Program of Zhejiang A&F University(No.113-2013200148)ZAFU-Institute of Termite Control of Yuhang cooperative project(2045200485,2045200529).
文摘In worldwide,the use of chemical pesticides to protect wood has been greatly restricted.In recent years,a large number of researchers devoted to the search for natural,safe and non-polluting bioactive chemical compounds from plants as an alternative to synthetic organic chemical preservative.In Chinese folk,tung oil can be used as paint for wooden furniture to protect them from pests.This study aimed to evaluate the chemical compositions of raw and heated tung oil and their activity against termite.In choice bioassays,weight loss of wood treated with 5%raw or heated tung oil after 4 weeks was significantly less than that of the control group.In no-choice bioassays,there was a significant difference in termite survival and wood weight loss on raw and heated tung oil-treated wood.When tung oil-treatment concentrations increased to 5%,wood weight loss was less than 10%.There was no significant difference in termite survival and wood weight loss between raw and heated tung oil-treated wood.Survival of termites in both tung oil wood treatments was significantly lower than that in the starvation control after 4 weeks.Raw and heated tung oil significantly improved the resistance of pine wood to termites,and have the potential for the development of natural wood preservatives.
基金supported by the National Natural Science Foundation of China(Grant Nos.62071451,62331025,and U21A20447)the National Key Research and Development Project(Grant No.2021YFC3002204)the CAMS Innovation Fund for Medical Sciences(Grant No.2019-I2M-5-019).
文摘Dementias such as Alzheimer disease(AD)and mild cognitive impairment(MCI)lead to problems with memory,language,and daily activities resulting from damage to neurons in the brain.Given the irreversibility of this neuronal damage,it is crucial to find a biomarker to distinguish individuals with these diseases from healthy people.In this study,we construct a brain function network based on electroencephalography data to study changes in AD and MCI patients.Using a graph-theoretical approach,we examine connectivity features and explore their contributions to dementia recognition at edge,node,and network levels.We find that connectivity is reduced in AD and MCI patients compared with healthy controls.We also find that the edge-level features give the best performance when machine learning models are used to recognize dementia.The results of feature selection identify the top 50 ranked edge-level features constituting an optimal subset,which is mainly connected with the frontal nodes.A threshold analysis reveals that the performance of edge-level features is more sensitive to the threshold for the connection strength than that of node-and network-level features.In addition,edge-level features with a threshold of 0 provide the most effective dementia recognition.The K-nearest neighbors(KNN)machine learning model achieves the highest accuracy of 0.978 with the optimal subset when the threshold is 0.Visualization of edge-level features suggests that there are more long connections linking the frontal region with the occipital and parietal regions in AD and MCI patients compared with healthy controls.Our codes are publicly available at https://github.com/Debbie-85/eeg-connectivity.
基金supported by the National Natural Science Foundation of China(No.52073198)Science Foundation of Jiangsu Province(BK20211598)+1 种基金“111”project,the China Postdoctoral Science Foundation(No.2021T140495),Collaborative Innovation Center of Suzhou Nano Science and Technology,Soochow Universitythe Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
文摘Fluorine substitution was applied to the donor and acceptor segments of block copolymers to understand the impact of molecular structure on photovoltaic block copolymers and explore efficient materials for single-component organic solar cells(SCOSCs).Along this line,three fluorinated block copolymers,namely PBDB-T-b-PTYF6,PM6-b-PTY6,and PM6-bPTYF6,derived from PBDB-T-b-PTY6 were designed and synthesized.The UV-Vis absorption,energy level,and thin-film morphology of these block copolymers were systematically characterized.All fluorinated block copolymers show narrow bandgap and improved crystallinity.An enhanced open-circuit voltage was observed in the SCOSC based on PM6-b-PTY6.However,SCOSCs based on all fluorinated block copolymers exhibited low short-circuit current due to energy level mismatch and therefore had low power conversion efficiency at around 4%.By contrast,the SCOSCs based on control block copolymer PBDB-T-b-PTY6 exhibited the highest power conversion efficiency approaching 10%,with a high short-circuit current of 18.57 mA/cm~2.Our study was the first to perform fluorination on photovoltaic block copolymers and provides insight into precisely controlling the polymer structure and understanding the structure-property relationship in SCOSCs based on block copolymers.
文摘The main causes of floods in small and medium rivers in Jiangxi Province are meteorological factors,rainstorm factors and topographic factors.Based on the data of more than 600 floods from 19 hydrological stations in small and medium rivers in Jiangxi Province,the characteristics of floods in small and medium rivers are analyzed. The results show that the floods in small and medium rivers in Jiangxi Province had characteristics of strong seasonality,high frequency and strong suddenness,and were mainly local floods;rainstorm floods during May-June lasted for a long term,and had double peaks,multi-peaks or a single peak with a long duration. During July-September,floods usually had a single peak,and the duration was relatively short.
基金This work was supported by the Shanghai Shenkang Hospital Development Center(SHDC2020CR3067B)Shanghai Municipal Commission of Health and Family Planning(2018ZHYL0219)Milstein medical Asian American partnership foundation(no award/grant number).
文摘Background and purpose Medullary infarction(MI)caused by spontaneous vertebral artery dissection(sVAD)is an important type of stroke.It is important to distinguish sVAD from other causes of stroke since the treatment strategies and prognosis were different between them.In this study,we aimed to explore the clinical and radiological features of MI in patients with acute MI caused by sVAD.Methods Patients with acute MI caused by sVAD and non-sVAD in a single tertiary hospital were enrolled from 2010 to 2020.Epidemiologic,clinical and image features were collected and analysed.MI lesions were categorised into three levels rostrocaudally and four arterial groups:anteromedial,anterolateral,lateral and posterior.Results A total of 128 patients with MI were enrolled with 47 cases of sVAD and 81 cases of non-sVAD.Patients with sVAD were younger than those with non-sVAD(med 44 years old vs 58 years old).The sVAD group was less likely to have hypertension(44.68%vs 67.90%;p=0.010)and diabetes(19.15%vs 45.69%;p=0.003),but more likely to have non-sudden onset(27.66%vs 9.87%,p=0.009),minor neck injury(19.15%vs 1.23%;p=0.001)and headache(46.81%vs 7.41%;p=0.000).Vertically,sVAD became more common in caudal medulla than in rostral medulla.Horizontally,the sVAD group was more likely to have lateral MI(91.48%vs 2.96%,p=0.000).In multivariable logistic regression analysis,age,non-sudden onset and headache were independently associated with sVAD with ORs of 0.935(95%CI 0.892 to 0.981,p=0.006),3.507(95%CI 1.060 to 11.599,p=0.040)and 5.426(95%CI 1.673 to 17.599,p=0.005).Conclusion sVAD was not uncommon in patients with MI,especially in patients with lateral MI.Young patients with headache and non-sudden onset should remind clinician the possibility of sVAD.
基金Independently cultivates innovation team program of Jinan,China,Grant/Award Number:2021GXRC048Shandong Provincial Natural Science Foundation,Grant/Award Number:ZR2020ZD14+1 种基金National Natural Science Foundation of China,Grant/Award Number:82270922National Key Research and Development Program of China,Grant/Award Number:2022YFA0806100。
文摘Background:Liver ischemia/reperfusion(I/R)injury is a complex and multifactorial pathophysiological process.It is well recognized that the membrane permeability transition pore(mPTP)opening of mitochondria plays a crucial role in cell death after I/R injury.Cyclophilin D(CypD)is a critical positive regulator of mPTP.However,the effect of CypD on the pathogenesis of liver I/R injury and whether CypD is a potential therapeutic target are still unclear.Methods:We constructed liver-specific CypD knockout and AAV8-peptidyl prolyl isomerase F(PPIF)overexpression mice.Then,a 70%liver I/R injury model was established in mice,with 90 min of ischemia and 6 h of reperfusion.The liver function was detected by the level of serum glutamic pyruvic transaminase(alanine transaminase)and glutamic oxaloacetic transaminase(aspartate aminotransferase),the liver damage score and degree of necrosis were measured by hematoxylin and eosin(H&E)staining of liver tissues.Reactive oxygen species(ROS)staining,apoptosis,and autophagy-related molecules were used to detect apoptosis and autophagy during liver I/R.Results:The liver-specific knockout of CypD alleviated necrosis and dysfunction in liver I/R injury,by reducing the excessive production of ROS,and inhibiting cell apoptosis and autophagy.On the contrary,overexpression of CypD exacerbated I/R-induced liver damage.Conclusion:We found that the downregulation of CypD expression alleviated liver I/R injury by reducing apoptosis and autophagy through caspase-3/Beclin1 crosstalk;in contrast,the upregulation of CypD expression aggravated liver I/R injury.Therefore,interfering with the expression of CypD seems to be a promising treatment for liver I/R injury.
基金funded by Capital Clinical Diagnosis and Treatment Technology Research and Demonstration Application Project of China(Grant No.Z191100006619029)National Key R&D Program of China(Grant No.2020YFC2008200).
文摘Background:To compare the efficacy and safety of preoperative intravitreal injections of ranibizumab and conbercept in Chinese proliferative diabetic retinopathy(PDR)patients.Methods:This prospective randomized controlled trial enrolled 90 eyes of 80 patients with PDR.Forty-four eyes of 40 patients that received intravitreal ranibizumab(IVR)injections(0.5 mg/0.05 mL)before vitreous surgeries were assigned to the IVR group.Forty-six eyes of 40 patients that received intravitreal conbercept(IVC)injections(0.5 mg/0.05 mL)before vitreous surgeries were assigned to the IVC group.Intraoperative and postoperative indices were assessed for further comparison between the two groups.Results:There were no statistically significant differences in all surgery indices,including intraoperative indices(surgery time,P=0.225;intraoperative bleeding,P=0.808;endodiathermy use,P=0.693;incidence of iatrogenic retinal breaks,P=0.740;relaxing retinotomy,P=0.682;retinal reattachment,P=0.682 and silicone oil tamponade,P=0.814)and postoperative indices(postoperative vitreous hemorrhage(VH),P=0.808;neovascular glaucoma(NVG),P=0.964;recurrent retinal detachment,P=0.531;postoperative fibrovascular proliferation progression,P=0.682 and reoperation,P=0.955)between the two groups.There were no statistically significant differences in best-corrected visual acuity(BCVA)at each follow-up visit(P=0.939,0.669,0.741 and 0.717,respectively)or in central retinal thickness(CRT)(P=0.976,0.699,0.551 and 0.686,respectively).As for safety profile,both groups had no ocular or system adverse events during the observation period.Conclusions:IVR and IVC as a pretreatment of vitrectomy had similar efficacy and safety profile for Chinese PDR patients.
基金the National Natural Science Foundation of China(Nos.22061003 and 21861006)Guangxi Natural Science Foundation of China(No.2019GXNSFAA245027)+2 种基金Guangxi Key R&D Program(No.AB18221005)Science and Technology Major Project of Guangxi(No.AA17204058-21)Guangxi Science and Technology Base,and Special Talents(No.AD 19110027)for financial support.
文摘Single-atom site(SAS)catalysts have attracted considerable attention due to their excellent performance.However,most of the current research models of SAS catalysts are based on inorganic catalysts,where“metal and coordination atom interaction”cannot simulate the fine-tuning effect of organic ligands on metal catalytic centers in homogeneous catalysts.Therefore,certain chemical transformations in homogeneous catalysis cannot be perfectly replicated.Here,we used porous organic ligand polymers as the carrier,which effectively changes the charge regulation of nanoparticles and monoatomic metal catalysts.Drawing lessons from traditional homogeneous metal/ligand catalysis,we introduced various functional groups into the ligand polymers to adjust the electronic properties,and successfully realized the hydrosilylation of internal alkynes with high catalytic performance.The selectivity and catalytic efficiency under the Pd@POL-1 catalyst system were improved compared with previous studies.The internal alkynes with various structures can complete this reaction,and the ratio of E/Zcan reach up to 100:1.
基金supported by the Science and Technology Commission of Shanghai Municipality(No.16JC1400603)
文摘Soft conductive films composed of a silver nanowire（AgNW） network, a neutral-pH PEDOT：PSS overcoating layer and a polydimethylsiloxane（PDMS） elastomer substrate are fabricated by large area compatible coating processes. The neutral-pH PEDOT：PSS layer is shown to be able to significantly improve the conductivity,stretchability and air stability of the conductive films. The soft conductive films are patterned using a simple maskless patterning approach to fabricate an 8 x 8 flexible pressure sensor array. It is shown that such soft conductive films can help to improve the sensitivity and reduce the signal crosstalk over the pressure sensor array.
