Tumor vaccine therapy offers significant advantages over conventional treatments,including reduced toxic side effects.However,it currently functions primarily as an adjuvant treatment modality in clinical oncology due...Tumor vaccine therapy offers significant advantages over conventional treatments,including reduced toxic side effects.However,it currently functions primarily as an adjuvant treatment modality in clinical oncology due to limitations in tumor antigen selection and delivery methods.Tumor vaccines often fail to elicit a sufficiently robust immune response against progressive tumors,thereby limiting their clinical efficacy.In this study,we developed a nanoparticle-based tumor vaccine,OVA@HA-PEI,utilizing ovalbumin(OVA)as the presenting antigen and hyaluronic acid(HA)and polyethyleneimine(PEI)as adjuvants and carriers.This formulation significantly enhanced the proliferation of immune cells and cytokines,such as CD3,CD8,interferon-,and tumor necrosis factor-,in vivo,effectively activating an immune response against B16–F10 tumors.In vivofluorescenceflow cytometry(IVFC)has already become an effective method for monitoring circulating tumor cells(CTCs)due to its direct,noninvasive,and long-term detection capabilities.Our study utilized a laboratory-constructed IVFC system to monitor the immune processes induced by the OVA@HA-PEI tumor vaccine and an anti-programmed death-1(PD-1)antibody.The results demonstrated that the combined treatment of OVA@HA-PEI and anti-PD-1 antibody significantly improved the survival time of mice compared to anti-PD-1 antibody treatment alone.Additionally,this combination therapy substantially reduced the number of CTCs in vivo,increased the clearance rate of CTCs by the immune system,and slowed tumor progression.Thesefindings greatly enhance the clinical application prospects of IVFC and tumor vaccines.展开更多
The ability to noninvasively manipulate and isolate specific cell populations in vivo is critical for advancing real-time diagnostics,precision medicine,and immunological research.Here,we present a novel and broadly a...The ability to noninvasively manipulate and isolate specific cell populations in vivo is critical for advancing real-time diagnostics,precision medicine,and immunological research.Here,we present a novel and broadly applicable optical trapping system based on a custom-designed 2×3 optical tweezer array,which enables the real-time interception and manipulation of circulating leukocytes in live animals.By utilizing intrinsic velocity differences between leukocytes and red blood cells,the system achieves stable trapping of individual leukocytes in vessels 15-20μm in diameter and decelerates multiple cells in vessels greater than 20μm.Notably,it also enables the optical blockage of lymphatic vessels exceeding 50μm,a previously unreported capability.This label-free,noninvasive approach operates without repeated blood draws and is compatible with diverse vessel geometries and flow dynamics.The system offers a generalizable solution for in vivo cell extraction and analysis,paving the way for high-precision single-cell technologies in biomedical research and clinical translation.展开更多
A concrete-filled double-skin tube(CFDST)is a new type of composite material.Experimental studies have been conducted to investigate the axial compression behavior of CFDST members for approximately 30 years.This pape...A concrete-filled double-skin tube(CFDST)is a new type of composite material.Experimental studies have been conducted to investigate the axial compression behavior of CFDST members for approximately 30 years.This paper provides a review of the status of axial compression bearing capacity tests conducted on circular CFDST stub columns as well as a summary of test data for 165 circular CFDST stub columns reported in 22 papers.A relatively complete high-quality test database is established.Based on this database,the main factors affecting the axial compression bearing capacity of the CFDST stub columns are analyzed.The prediction accuracy and robustness of an existing theoretical prediction model,which is a data-driven model,are evaluated,and a numerical simulation of the axial compression bearing capacity of the CFDST stub columns is conducted.In addition,the differences between the basic theory and experimental results of various models are compared,and the possible sources of prediction errors are analyzed.The current model for predicting the axial compression capacity of CFDST stub columns cannot simultaneously satisfy the requirements of high accuracy and confidence,and the stress independency assumption introduced in the test is not valid.The main error source in the theoretical prediction model is the non-simultaneous consideration of the effects of the void ratio and inner steel tube.展开更多
基金supported by the National Key Research and Development Program of China,Grant Number:2021YFF0502900,2019YFC1604604National Natural Science Foundation of China,Grant Number:62075013,62027824.
文摘Tumor vaccine therapy offers significant advantages over conventional treatments,including reduced toxic side effects.However,it currently functions primarily as an adjuvant treatment modality in clinical oncology due to limitations in tumor antigen selection and delivery methods.Tumor vaccines often fail to elicit a sufficiently robust immune response against progressive tumors,thereby limiting their clinical efficacy.In this study,we developed a nanoparticle-based tumor vaccine,OVA@HA-PEI,utilizing ovalbumin(OVA)as the presenting antigen and hyaluronic acid(HA)and polyethyleneimine(PEI)as adjuvants and carriers.This formulation significantly enhanced the proliferation of immune cells and cytokines,such as CD3,CD8,interferon-,and tumor necrosis factor-,in vivo,effectively activating an immune response against B16–F10 tumors.In vivofluorescenceflow cytometry(IVFC)has already become an effective method for monitoring circulating tumor cells(CTCs)due to its direct,noninvasive,and long-term detection capabilities.Our study utilized a laboratory-constructed IVFC system to monitor the immune processes induced by the OVA@HA-PEI tumor vaccine and an anti-programmed death-1(PD-1)antibody.The results demonstrated that the combined treatment of OVA@HA-PEI and anti-PD-1 antibody significantly improved the survival time of mice compared to anti-PD-1 antibody treatment alone.Additionally,this combination therapy substantially reduced the number of CTCs in vivo,increased the clearance rate of CTCs by the immune system,and slowed tumor progression.Thesefindings greatly enhance the clinical application prospects of IVFC and tumor vaccines.
基金funding from the National Key Research and Development Program of China(2021YFF0502900)special fund for Research on the National Major Research Instruments of China(62027824)+2 种基金the National Natural Science Foundation of China(U24A20314)the Key Research and Development Program of Anhui Province in China(2022a05020028)the Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province.
文摘The ability to noninvasively manipulate and isolate specific cell populations in vivo is critical for advancing real-time diagnostics,precision medicine,and immunological research.Here,we present a novel and broadly applicable optical trapping system based on a custom-designed 2×3 optical tweezer array,which enables the real-time interception and manipulation of circulating leukocytes in live animals.By utilizing intrinsic velocity differences between leukocytes and red blood cells,the system achieves stable trapping of individual leukocytes in vessels 15-20μm in diameter and decelerates multiple cells in vessels greater than 20μm.Notably,it also enables the optical blockage of lymphatic vessels exceeding 50μm,a previously unreported capability.This label-free,noninvasive approach operates without repeated blood draws and is compatible with diverse vessel geometries and flow dynamics.The system offers a generalizable solution for in vivo cell extraction and analysis,paving the way for high-precision single-cell technologies in biomedical research and clinical translation.
基金funded by the National Natural Science Foundation of China(Grant No.51268044).
文摘A concrete-filled double-skin tube(CFDST)is a new type of composite material.Experimental studies have been conducted to investigate the axial compression behavior of CFDST members for approximately 30 years.This paper provides a review of the status of axial compression bearing capacity tests conducted on circular CFDST stub columns as well as a summary of test data for 165 circular CFDST stub columns reported in 22 papers.A relatively complete high-quality test database is established.Based on this database,the main factors affecting the axial compression bearing capacity of the CFDST stub columns are analyzed.The prediction accuracy and robustness of an existing theoretical prediction model,which is a data-driven model,are evaluated,and a numerical simulation of the axial compression bearing capacity of the CFDST stub columns is conducted.In addition,the differences between the basic theory and experimental results of various models are compared,and the possible sources of prediction errors are analyzed.The current model for predicting the axial compression capacity of CFDST stub columns cannot simultaneously satisfy the requirements of high accuracy and confidence,and the stress independency assumption introduced in the test is not valid.The main error source in the theoretical prediction model is the non-simultaneous consideration of the effects of the void ratio and inner steel tube.
