对棉铃虫Helicoverpa ar migera核型多角体病毒HearSNPV的ORF33基因(ha33)进行克隆和原核表达,hass在E.coli中表达不完全,表达产物的大小为17kDa,小于预测的分子量28.4kDa。用纯化的原核表达产物免疫家兔,制备了多克隆抗体,应用多克隆...对棉铃虫Helicoverpa ar migera核型多角体病毒HearSNPV的ORF33基因(ha33)进行克隆和原核表达,hass在E.coli中表达不完全,表达产物的大小为17kDa,小于预测的分子量28.4kDa。用纯化的原核表达产物免疫家兔,制备了多克隆抗体,应用多克隆抗体检测了HearSNPV感染的宿主细胞(HzAMI)中ORF33基因的表达,表达产物的分子量为31kDa。并通过共聚焦荧光显微镜方法,用多克隆抗体检测编码的蛋白在宿主细胞(HzAM1)中的亚细胞定位,发现ha33编码的蛋白存在于宿主细胞的细胞质中,并持续到感染后期。展开更多
The study of radiation damage of high- molecular weight substances due to MeV ion interactions is of interest for engineering and scientific applications. In the present study polystyrene (PS) was irradiated with 107A...The study of radiation damage of high- molecular weight substances due to MeV ion interactions is of interest for engineering and scientific applications. In the present study polystyrene (PS) was irradiated with 107Ag ions of three different charge states (q) 11+, 14+ and 25+ and of 130 MeV energy. The emission of hydrogen from PS was monitored as a function of the incident ion fluence. The experimental results showed that the hydrogen depletion per incident ion from PS varies as qn, where n was found to be 2.1 as compared to the value 2.7 to 3.0 reported in the literature. Radii of the nanometric damaged zones or ion tracks formed were analyzed from the slope of the hydrogen depletion versus ion fluence curves as a function of charge state of incident ion. These have values between 3.2 - 6.8 nm. These radii were found to depend upon the charge state of the incident ion and vary as qm, where m has the value 0.9.展开更多
This work was aimed to investigate the changes brought about in the polymer polycarbonate irradiated to different doses of γ-radiation. Yellowing of the samples with the increase of γ-absorbed dose was observed. The...This work was aimed to investigate the changes brought about in the polymer polycarbonate irradiated to different doses of γ-radiation. Yellowing of the samples with the increase of γ-absorbed dose was observed. The changes in the optical properties were studied by recording UV-Visible absorbance spectra of the pristine and irradiated polycarbonate films. A simultaneous coexistence of direct and indirect band gaps was observed. The indirect band gap values were found lower in comparison to the corresponding values of direct band gap in the pristine and γ-irradiated poly-carbonate. Both types of the optical band gap energies had decreasing tendency with the increasing γ-radiation dose. Urbach energy was also determined from the tail of absorption edge which was found to have increasing tendency with progressive γ-radiation dose. Increase in carbon cluster size with the increasing γ absorbed dose was also shown. This increase in the number of carbon atoms (N) in a cluster can be correlated to the optical energy band gap (Eg). Moreover, the FTIR spectra of pristine and irradiated PC samples suggest chain scissoring with apparently the elimination of carbon di/monoxide.展开更多
The CSR External-target Experiment(CEE)[1]will be the first large scale experiment in nuclear physics independently developed in China covering the GeV energy regime.With〜1 m^3 volume,the time projection chamber(TPC)o...The CSR External-target Experiment(CEE)[1]will be the first large scale experiment in nuclear physics independently developed in China covering the GeV energy regime.With〜1 m^3 volume,the time projection chamber(TPC)of CEE will be the largest TPC constructed and used in China.展开更多
文摘对棉铃虫Helicoverpa ar migera核型多角体病毒HearSNPV的ORF33基因(ha33)进行克隆和原核表达,hass在E.coli中表达不完全,表达产物的大小为17kDa,小于预测的分子量28.4kDa。用纯化的原核表达产物免疫家兔,制备了多克隆抗体,应用多克隆抗体检测了HearSNPV感染的宿主细胞(HzAMI)中ORF33基因的表达,表达产物的分子量为31kDa。并通过共聚焦荧光显微镜方法,用多克隆抗体检测编码的蛋白在宿主细胞(HzAM1)中的亚细胞定位,发现ha33编码的蛋白存在于宿主细胞的细胞质中,并持续到感染后期。
文摘The study of radiation damage of high- molecular weight substances due to MeV ion interactions is of interest for engineering and scientific applications. In the present study polystyrene (PS) was irradiated with 107Ag ions of three different charge states (q) 11+, 14+ and 25+ and of 130 MeV energy. The emission of hydrogen from PS was monitored as a function of the incident ion fluence. The experimental results showed that the hydrogen depletion per incident ion from PS varies as qn, where n was found to be 2.1 as compared to the value 2.7 to 3.0 reported in the literature. Radii of the nanometric damaged zones or ion tracks formed were analyzed from the slope of the hydrogen depletion versus ion fluence curves as a function of charge state of incident ion. These have values between 3.2 - 6.8 nm. These radii were found to depend upon the charge state of the incident ion and vary as qm, where m has the value 0.9.
文摘This work was aimed to investigate the changes brought about in the polymer polycarbonate irradiated to different doses of γ-radiation. Yellowing of the samples with the increase of γ-absorbed dose was observed. The changes in the optical properties were studied by recording UV-Visible absorbance spectra of the pristine and irradiated polycarbonate films. A simultaneous coexistence of direct and indirect band gaps was observed. The indirect band gap values were found lower in comparison to the corresponding values of direct band gap in the pristine and γ-irradiated poly-carbonate. Both types of the optical band gap energies had decreasing tendency with the increasing γ-radiation dose. Urbach energy was also determined from the tail of absorption edge which was found to have increasing tendency with progressive γ-radiation dose. Increase in carbon cluster size with the increasing γ absorbed dose was also shown. This increase in the number of carbon atoms (N) in a cluster can be correlated to the optical energy band gap (Eg). Moreover, the FTIR spectra of pristine and irradiated PC samples suggest chain scissoring with apparently the elimination of carbon di/monoxide.
基金National Natural Science Foundation of China(11927901)。
文摘The CSR External-target Experiment(CEE)[1]will be the first large scale experiment in nuclear physics independently developed in China covering the GeV energy regime.With〜1 m^3 volume,the time projection chamber(TPC)of CEE will be the largest TPC constructed and used in China.
