No-wash bioassays based on nanoparticles are used widely in biochemical procedures because of their responsive sensing and no need forwashing processes.Essential for no-wash biosensing are the interactions between nan...No-wash bioassays based on nanoparticles are used widely in biochemical procedures because of their responsive sensing and no need forwashing processes.Essential for no-wash biosensing are the interactions between nanoparticles and biomolecules,but it is challenging toachieve controlled bioconjugation of molecules on nanomaterials.Reported here is a way to actively improve nanoparticle-based no-washbioassays by enhancing the binding between biomolecules and gold nanoparticles via acoustic streaming generated by a gigahertz piezoelectricnanoelectromechanical resonator.Tunable micro-vortices are generated at the device-liquid interface,thereby accelerating the internalcirculating flow of the solution,bypassing the diffusion limitation,and thus improving the binding between the biomolecules and goldnanoparticles.Combined with fluorescence quenching,an enhanced and ultrafast no-wash biosensing assay is realized for specific proteins.The sensing method presented here is a versatile tool for different types of biomolecule detection with high efficiency and simplicity.展开更多
Contactless acoustic manipulation of micro/nanoscale particles has attracted considerable attention owing to its near independence of the physical and chemical properties of the targets,making it universally applicabl...Contactless acoustic manipulation of micro/nanoscale particles has attracted considerable attention owing to its near independence of the physical and chemical properties of the targets,making it universally applicable to almost all biological systems.Thin-film bulk acoustic wave(BAW)resonators operating at gigahertz(GHz)frequencies have been demonstrated to generate localized high-speed microvortices through acoustic streaming effects.Benefitting from the strong drag forces of the high-speed vortices,BAW-enabled GHz acoustic streaming tweezers(AST)have been applied to the trapping and enrichment of particles ranging in size from micrometers to less than 100 nm.However,the behavior of particles in such 3D microvortex systems is still largely unknown.In this work,the particle behavior(trapping,enrichment,and separation)in GHz AST is studied by theoretical analyses,3D simulations,and microparticle tracking experiments.It is found that the particle motion in the vortices is determined mainly by the balance between the acoustic streaming drag force and the acoustic radiation force.This work can provide basic design principles for AST-based lab-on-a-chip systems for a variety of applications.展开更多
Background:Chronic kidney disease(CKD)-associated anemia(CKD-anemia)is associated with poor survival,and hemoglobin targets are often not achieved with current therapies.Phase 3 trials have demonstrated the treatment ...Background:Chronic kidney disease(CKD)-associated anemia(CKD-anemia)is associated with poor survival,and hemoglobin targets are often not achieved with current therapies.Phase 3 trials have demonstrated the treatment efficacy of roxadustat for CKD-anemia.This phase 4 study aims to evaluate the long-term(52-week)safety and effectiveness of roxadustat in a broad real-world patient population with CKD-anemia with and without dialysis in China.Methods:This Phase 4 multicenter,open-label,prospective study,conducted from 24 November 2020 to 11 November 2022,evaluated the long-term safety and effectiveness of roxadustat for CKD-anemia in China.Patients aged≥18 years with CKD-anemia with or without dialysis were included.The initial oral dose was 70-120 mg(weight-based followed by dose adjustment)over 52 weeks.The primary endpoint was safety based on adverse events(AEs).The secondary endpoints were hemoglobin changes from baseline and the proportion of patients who achieved mean hemoglobin≥100 g/L.Effectiveness evaluable populations 1(EE1)and EE2 included roxadustat-naïve and previously roxadustat-treated patients,respectively.The safety analysis set(SAF)included all patients who received≥1 occasion.Results:The EE1,EE2,and SAF populations included 1804,193,and 2021 patients,respectively.In the SAF,the mean age was 50±14 years,and 1087 patients(53.8%)were male.Mean baseline hemoglobin was 96.9±14.0 g/L in EE1 and 100.3±12.9 g/L in EE2.In EE1,the mean(95%confidence interval)hemoglobin changes from baseline over weeks 24-36 and 36-52 were 14.2(13.5-14.9)g/L and 14.3(13.5-15.0)g/L,respectively.Over weeks 24-36 and 36-52,83.3%and 86.1%of patients in EE1 and 82.7%and 84.7%in EE2 achieved mean hemoglobin≥100 g/L,respectively.In the SAF,1643(81.3%)patients experienced treatment-emergent AEs(TEAEs).Overall,219(10.8%)patients experienced drug-related TEAEs.Thirty-eight(1.9%)patients died of TEAEs(unrelated to the study drug).Vascular access thrombosis was uncommon.Conclusions:Roxadustat(52 weeks)increased hemoglobin and maintained the treatment target in Chinese patients with CKD-anemia with acceptable safety,supporting its use in real-world settings.展开更多
基金the financial support received from the National Natural Science Foundation of China(Grant No.62174119)the 111 Project (Grant No.B07014)the Foundation for Talent Scientists of Nanchang Institute for Microtechnology of Tianjin University
文摘No-wash bioassays based on nanoparticles are used widely in biochemical procedures because of their responsive sensing and no need forwashing processes.Essential for no-wash biosensing are the interactions between nanoparticles and biomolecules,but it is challenging toachieve controlled bioconjugation of molecules on nanomaterials.Reported here is a way to actively improve nanoparticle-based no-washbioassays by enhancing the binding between biomolecules and gold nanoparticles via acoustic streaming generated by a gigahertz piezoelectricnanoelectromechanical resonator.Tunable micro-vortices are generated at the device-liquid interface,thereby accelerating the internalcirculating flow of the solution,bypassing the diffusion limitation,and thus improving the binding between the biomolecules and goldnanoparticles.Combined with fluorescence quenching,an enhanced and ultrafast no-wash biosensing assay is realized for specific proteins.The sensing method presented here is a versatile tool for different types of biomolecule detection with high efficiency and simplicity.
基金The authors gratefully acknowledge financial support from the National Key R&D Program of China(2018YFE0118700)the Natural Science Foundation of China(NSFC No.62174119)+1 种基金Tianjin Applied Basic Research and Advanced Technology(17JCJQJC43600)the 111 Project(B07014).
文摘Contactless acoustic manipulation of micro/nanoscale particles has attracted considerable attention owing to its near independence of the physical and chemical properties of the targets,making it universally applicable to almost all biological systems.Thin-film bulk acoustic wave(BAW)resonators operating at gigahertz(GHz)frequencies have been demonstrated to generate localized high-speed microvortices through acoustic streaming effects.Benefitting from the strong drag forces of the high-speed vortices,BAW-enabled GHz acoustic streaming tweezers(AST)have been applied to the trapping and enrichment of particles ranging in size from micrometers to less than 100 nm.However,the behavior of particles in such 3D microvortex systems is still largely unknown.In this work,the particle behavior(trapping,enrichment,and separation)in GHz AST is studied by theoretical analyses,3D simulations,and microparticle tracking experiments.It is found that the particle motion in the vortices is determined mainly by the balance between the acoustic streaming drag force and the acoustic radiation force.This work can provide basic design principles for AST-based lab-on-a-chip systems for a variety of applications.
文摘Background:Chronic kidney disease(CKD)-associated anemia(CKD-anemia)is associated with poor survival,and hemoglobin targets are often not achieved with current therapies.Phase 3 trials have demonstrated the treatment efficacy of roxadustat for CKD-anemia.This phase 4 study aims to evaluate the long-term(52-week)safety and effectiveness of roxadustat in a broad real-world patient population with CKD-anemia with and without dialysis in China.Methods:This Phase 4 multicenter,open-label,prospective study,conducted from 24 November 2020 to 11 November 2022,evaluated the long-term safety and effectiveness of roxadustat for CKD-anemia in China.Patients aged≥18 years with CKD-anemia with or without dialysis were included.The initial oral dose was 70-120 mg(weight-based followed by dose adjustment)over 52 weeks.The primary endpoint was safety based on adverse events(AEs).The secondary endpoints were hemoglobin changes from baseline and the proportion of patients who achieved mean hemoglobin≥100 g/L.Effectiveness evaluable populations 1(EE1)and EE2 included roxadustat-naïve and previously roxadustat-treated patients,respectively.The safety analysis set(SAF)included all patients who received≥1 occasion.Results:The EE1,EE2,and SAF populations included 1804,193,and 2021 patients,respectively.In the SAF,the mean age was 50±14 years,and 1087 patients(53.8%)were male.Mean baseline hemoglobin was 96.9±14.0 g/L in EE1 and 100.3±12.9 g/L in EE2.In EE1,the mean(95%confidence interval)hemoglobin changes from baseline over weeks 24-36 and 36-52 were 14.2(13.5-14.9)g/L and 14.3(13.5-15.0)g/L,respectively.Over weeks 24-36 and 36-52,83.3%and 86.1%of patients in EE1 and 82.7%and 84.7%in EE2 achieved mean hemoglobin≥100 g/L,respectively.In the SAF,1643(81.3%)patients experienced treatment-emergent AEs(TEAEs).Overall,219(10.8%)patients experienced drug-related TEAEs.Thirty-eight(1.9%)patients died of TEAEs(unrelated to the study drug).Vascular access thrombosis was uncommon.Conclusions:Roxadustat(52 weeks)increased hemoglobin and maintained the treatment target in Chinese patients with CKD-anemia with acceptable safety,supporting its use in real-world settings.
