Pore architecture in bioceramic scaffolds plays an important role in facilitating vascularization efficiency during bone repair or orbital reconstruction.Many investigations have explored this relationship but lack in...Pore architecture in bioceramic scaffolds plays an important role in facilitating vascularization efficiency during bone repair or orbital reconstruction.Many investigations have explored this relationship but lack integrating pore architectural features in a scaffold,hindering optimization of architectural parameters(geometry,size and curvature)to improve vascularization and consequently clinical outcomes.To address this challenge,we have developed an integrating design strategy to fabricate different pore architectures(cube,gyroid and hexagon)with different pore dimensions(-350,500 and 650 lm)in the silicate-based bioceramic scaffolds via digital light processing technique.The sintered scaffolds maintained high-fidelity pore architectures similar to the printing model.The hexagon-and gyroid-pore scaffolds exhibited the highest and lowest compressive strength(from 15 to 55MPa),respectively,but the cube-pore scaffolds showed appreciable elastic modulus.Moreover,the gyroid-pore architecture contributed on a faster ion dissolution and mass decay in vitro.It is interesting that bothμCT and histological analyses indicate vascularization efficiency was challenged even in the 650-μm pore region of hexagon-pore scaffolds within 2weeks in rabbit models,but the gyroid-pore constructs indicated appreciable blood vessel networks even in the 350-μm pore region at 2weeks and high-density blood vessels were uniformly invaded in the 500-and 650-μm pore at 4weeks.Angiogenesis was facilitated in the cube-pore scaffolds in comparison with the hexagon-pore ones within 4weeks.These studies demonstrate that the continuous pore wall curvature feature in gyroid-pore architecture is an important implication for biodegradation,vascular cell migration and vessel ingrowth in porous bioceramic scaffolds.展开更多
基金financial support from the National Key Research and Development Program of China(2017YFE0117700)the National Natural Science Foundation of China(81871775,81902225,81772311)+1 种基金Zhejiang Provincial Natural Science Foundation of China(LBY21H060001,LGF21H060002,Z22E029971)the Medical and Health Research Project of Zhejiang Province(2020KY929,2020RC115).
文摘Pore architecture in bioceramic scaffolds plays an important role in facilitating vascularization efficiency during bone repair or orbital reconstruction.Many investigations have explored this relationship but lack integrating pore architectural features in a scaffold,hindering optimization of architectural parameters(geometry,size and curvature)to improve vascularization and consequently clinical outcomes.To address this challenge,we have developed an integrating design strategy to fabricate different pore architectures(cube,gyroid and hexagon)with different pore dimensions(-350,500 and 650 lm)in the silicate-based bioceramic scaffolds via digital light processing technique.The sintered scaffolds maintained high-fidelity pore architectures similar to the printing model.The hexagon-and gyroid-pore scaffolds exhibited the highest and lowest compressive strength(from 15 to 55MPa),respectively,but the cube-pore scaffolds showed appreciable elastic modulus.Moreover,the gyroid-pore architecture contributed on a faster ion dissolution and mass decay in vitro.It is interesting that bothμCT and histological analyses indicate vascularization efficiency was challenged even in the 650-μm pore region of hexagon-pore scaffolds within 2weeks in rabbit models,but the gyroid-pore constructs indicated appreciable blood vessel networks even in the 350-μm pore region at 2weeks and high-density blood vessels were uniformly invaded in the 500-and 650-μm pore at 4weeks.Angiogenesis was facilitated in the cube-pore scaffolds in comparison with the hexagon-pore ones within 4weeks.These studies demonstrate that the continuous pore wall curvature feature in gyroid-pore architecture is an important implication for biodegradation,vascular cell migration and vessel ingrowth in porous bioceramic scaffolds.
