[Objectives]To further explore the mechanism of quercetin regulating the activity of Sune-1 cells.[Methods]High-throughput mRNA-miRNA transcriptome sequencing technology was used to screen miRNA in Sune-1 cells treate...[Objectives]To further explore the mechanism of quercetin regulating the activity of Sune-1 cells.[Methods]High-throughput mRNA-miRNA transcriptome sequencing technology was used to screen miRNA in Sune-1 cells treated with quercetin.[Results]Statistical analysis showed that 1264 miRNAs were differentially expressed in Sune-1 cells treated with quercetin,of which 716 were significantly up-regulated and 548 were significantly down-regulated;191 miRNAs were differentially expressed in Sune-1 cells treated with quercetin,of which 129 were significantly up-regulated and 62 were significantly down-regulated.By comparing the expression differences of these mRNAs and miRNAs in different samples,six different expression patterns were clustered.The expression of the above miRNAs was verified by real-time quantitative PCR(qPCR),and the results were highly consistent with the transcriptome sequencing data.In addition,Gene Ontology annotation and functional enrichment analysis of miRNA target genes showed that CTGF,VHL and H19,which are related to the regulation of cell proliferation signal transduction,were predicted to be new targets of differential miRNAs such as miR494-3p and miR675-3p and may play an important regulatory role in the process of Quercetin inhibiting the proliferation of Sune-1 cells.[Conclusions]This study provides a basis for the rational use of anti-tumor functional components of traditional Chinese medicine,and also provides a theoretical basis for the targeted therapy of nasopharyngeal carcinoma.展开更多
Biosensors based on acetylcholinesterase(AChE)are crucial for early diagnosis,less invasive treatment,and drug evaluation of Alzheimer’s disease(AD).However,existing technologies often suffer from enzyme conformation...Biosensors based on acetylcholinesterase(AChE)are crucial for early diagnosis,less invasive treatment,and drug evaluation of Alzheimer’s disease(AD).However,existing technologies often suffer from enzyme conformational changes,leading to altered activity and loss and reduced sensor efficacy.To address this challenge,we developed a novel right-side-out-oriented red blood cell membrane-coated electrochemical biosensors(ROCMCBs)to evaluate AChE inhibitors from traditional Chinese medicines(TCMs)as potential anti-AD agents.The developed right-side-out-oriented coating based on immunoaf-finity not only fully exposed the binding sites of AChE on the cell membrane but also ensured its confor-mation and stability as a peripheral membrane-anchoring protein,which was conducive to maintaining its biological activity and producing optimal interaction with drugs.At the same time,the biosensors exhib-ited a satisfactory sensitivity(limit of detection�0.41 pmol/L).Ultimately,six potentially active com-pounds against AD(baicalin,geniposide,gastrodin,berberine,rhynchophylline,and senkyunolide A)were rapidly identified and evaluated from TCMs.This project provides a promising strategy for devel-oping cell membrane-coated electrochemical biosensors.The application of cell membrane-coated elec-trochemical biosensors with well-defined cell membrane orientation further expands new perspectives and methods for AChE-targeted anti-AD research.展开更多
基金Supported by Educational Research Project for Young and Middle-aged Teachers in Fujian Province(Science and Technology Category,JAT210477)。
文摘[Objectives]To further explore the mechanism of quercetin regulating the activity of Sune-1 cells.[Methods]High-throughput mRNA-miRNA transcriptome sequencing technology was used to screen miRNA in Sune-1 cells treated with quercetin.[Results]Statistical analysis showed that 1264 miRNAs were differentially expressed in Sune-1 cells treated with quercetin,of which 716 were significantly up-regulated and 548 were significantly down-regulated;191 miRNAs were differentially expressed in Sune-1 cells treated with quercetin,of which 129 were significantly up-regulated and 62 were significantly down-regulated.By comparing the expression differences of these mRNAs and miRNAs in different samples,six different expression patterns were clustered.The expression of the above miRNAs was verified by real-time quantitative PCR(qPCR),and the results were highly consistent with the transcriptome sequencing data.In addition,Gene Ontology annotation and functional enrichment analysis of miRNA target genes showed that CTGF,VHL and H19,which are related to the regulation of cell proliferation signal transduction,were predicted to be new targets of differential miRNAs such as miR494-3p and miR675-3p and may play an important regulatory role in the process of Quercetin inhibiting the proliferation of Sune-1 cells.[Conclusions]This study provides a basis for the rational use of anti-tumor functional components of traditional Chinese medicine,and also provides a theoretical basis for the targeted therapy of nasopharyngeal carcinoma.
基金the National Natural Science Foun-dation of China(Nos.82104125 and 82373832)China Post-doctoral Science Foundation(No.2021M702632)Key Research and Development Projects of Shaanxi Province(Nos.2023-YBSF-145,2024SF-YBXM-469,2024JC-YBMS-651).
文摘Biosensors based on acetylcholinesterase(AChE)are crucial for early diagnosis,less invasive treatment,and drug evaluation of Alzheimer’s disease(AD).However,existing technologies often suffer from enzyme conformational changes,leading to altered activity and loss and reduced sensor efficacy.To address this challenge,we developed a novel right-side-out-oriented red blood cell membrane-coated electrochemical biosensors(ROCMCBs)to evaluate AChE inhibitors from traditional Chinese medicines(TCMs)as potential anti-AD agents.The developed right-side-out-oriented coating based on immunoaf-finity not only fully exposed the binding sites of AChE on the cell membrane but also ensured its confor-mation and stability as a peripheral membrane-anchoring protein,which was conducive to maintaining its biological activity and producing optimal interaction with drugs.At the same time,the biosensors exhib-ited a satisfactory sensitivity(limit of detection�0.41 pmol/L).Ultimately,six potentially active com-pounds against AD(baicalin,geniposide,gastrodin,berberine,rhynchophylline,and senkyunolide A)were rapidly identified and evaluated from TCMs.This project provides a promising strategy for devel-oping cell membrane-coated electrochemical biosensors.The application of cell membrane-coated elec-trochemical biosensors with well-defined cell membrane orientation further expands new perspectives and methods for AChE-targeted anti-AD research.
