This study developed a food-grade Pickering emulsions delivery system stabilized by soybean protein isolate(SPI)-dextran conjugate to enhance the anti-inflammatory effects of hesperidin(HES)on ulcerative colitis.The P...This study developed a food-grade Pickering emulsions delivery system stabilized by soybean protein isolate(SPI)-dextran conjugate to enhance the anti-inflammatory effects of hesperidin(HES)on ulcerative colitis.The Pickering emulsions achieved a high encapsulation efficiency for HES(82.19%±1.47%).The encapsulated HES exhibited significantly enhanced antioxidant capacity,with an approximately 1.5-fold increase in DPPH scav-enging and a 7.2-fold increase in hydroxyl radical scavenging.Furthermore,they provided substantial protective effects,significantly improving HES retention during storage and increasing its in vitro bioaccessibility by 45.84%.In vivo mouse trial revealed that HES-loaded Pickering emulsions and their fortified soymilk effectively mitigated the inflammatory response of ulcerative colitis via protecting the normal morphological function of mouse colon,decreasing the level of pro-inflammatory factors,and improving the stability of intestinal micro-biota.Collectively,SPI-dextran conjugate-stabilized Pickering emulsions and fortified soymilk enhanced the anti-inflammatory effect of HES,highlighting a great application potential in functional foods.展开更多
基金supported by the Opening Foundation of Key Laboratory of Coarse Cereal Processing,Ministry of Agriculture and Rural Affairs(No.2025CC003).
文摘This study developed a food-grade Pickering emulsions delivery system stabilized by soybean protein isolate(SPI)-dextran conjugate to enhance the anti-inflammatory effects of hesperidin(HES)on ulcerative colitis.The Pickering emulsions achieved a high encapsulation efficiency for HES(82.19%±1.47%).The encapsulated HES exhibited significantly enhanced antioxidant capacity,with an approximately 1.5-fold increase in DPPH scav-enging and a 7.2-fold increase in hydroxyl radical scavenging.Furthermore,they provided substantial protective effects,significantly improving HES retention during storage and increasing its in vitro bioaccessibility by 45.84%.In vivo mouse trial revealed that HES-loaded Pickering emulsions and their fortified soymilk effectively mitigated the inflammatory response of ulcerative colitis via protecting the normal morphological function of mouse colon,decreasing the level of pro-inflammatory factors,and improving the stability of intestinal micro-biota.Collectively,SPI-dextran conjugate-stabilized Pickering emulsions and fortified soymilk enhanced the anti-inflammatory effect of HES,highlighting a great application potential in functional foods.
