Calycosin,Astragali Radix most prominent ingredient,has drawn more attention as a result of its ability to treat atherosclerosis(AS).However,the mechanism of action has not been fully elucidated.We investigated the ef...Calycosin,Astragali Radix most prominent ingredient,has drawn more attention as a result of its ability to treat atherosclerosis(AS).However,the mechanism of action has not been fully elucidated.We investigated the effects of calycosin on bile acid(BA)metabolism and gut microbiome in ApoE^(-/-)mice fed a high-fat diet(HFD).The data showed that the aorta of ApoE^(-/-)mice treated with HFD showed significant atheromatous plaque formation and lipid accumulation,and the levels of total cholesterol(TC),triglycerides(TG)and low-density lipoprotein cholesterol(LDL-C)were significantly increased,while the levels of high-density lipoprotein cholesterol(HDL-C)were significantly decreased.Calycosin can substantially regulate lipid levels,thereby alleviating liver lipid deposition induced by atherosclerosis.In addition,16S rRNA sequencing showed that calycosin treatment has reshaped the gut microbiota disturbed by HFD,in particular,increasing the ratio of Bacteroidetes/Firmicutes,and improving the relative abundance of Bilophila,Desulfovibrio,Bacteroides,Lactobacillus,etc.Meanwhile,targeted metabolomics analysis showed that calycosin treatment significantly modulated glycodeoxycholic acid(CDCA),taurocholic acids(TCA),lithocholic acid(LCA),deoxycholic acid(DCA),taurodeoxycholic acid(TDCA)and BA pool composition,which were associated with atherosclerotic plaque areas.In addition,calycosin treatment also down-regulated farnesoid X receptor(FXR)protein levels and up-regulated cytochrome P450 family 7 subfamily A member 1(CYP7A1)protein levels in the hepatic.At the same time,calycosin inhibits the ileum FXR/TGR5 signaling pathway,inhibits BA reabsorption,promotes BA excretion,and reduces hepatic cholesterol accumulation by enterohepatic circulation.In addition,we found that calycosin significantly promoted the expression of hepatic ATP-binding cassette transporter A1(ABCA1)and ABCG1 to mediate cholesterol efflux.Meanwhile,calycosin regulates gut microbiota,and Bacteroides,Alistipes,Desulfovibrio,Lactobacillus,Bilophila and Odoribacter are closely related to specific BAs.This enables us to further understand the relationship between BA metabolism and gut microbiota.Calycosin may reduce high-fat diet-induced hepatic cholesterol accumulation in ApoE^(-/-)mice through gut microbiota and BA metabolism,and play a role in treating AS.Finally,we confirmed that calycosin-altered gut microbiota by fecal microbiota transplantation was sufficient to alleviate atherosclerosis.Taken together,our findings provide important insights into the pharmacological mechanisms underlying the efficacy of calycosin on atherosclerosis.展开更多
Gentianine has been shown to have a protective effect on hippocampal CA1 neurons in rats subjected to recurrent febrile convulsion（FC）.The present study sought to explore the possible mechanism of gentianine by intr...Gentianine has been shown to have a protective effect on hippocampal CA1 neurons in rats subjected to recurrent febrile convulsion（FC）.The present study sought to explore the possible mechanism of gentianine by intraperitoneally injecting gentianine into rats with warm water-induced FC.The results revealed that neuronal organelle injury was slightly ameliorated in the hippocampal CA1 region.The level of glutamate was decreased,but the level of γ-aminobutyric acid was increased,as detected by ninhydrin staining.In addition,glutamate acid decarboxylase expression in hippocampal CA1 was increased,as determined by immunohistochemistry.The results demonstrated that gentianine can ameliorate FC-induced neuronal injury by enhancing glutamate acid decarboxylase activity,decreasing glutamate levels and increasing γ-aminobutyric acid levels.展开更多
Mobile edge computing has emerged as a new paradigm to enhance computing capabilities by offloading complicated tasks to nearby cloud server.To conserve energy as well as maintain quality of service,low time complexit...Mobile edge computing has emerged as a new paradigm to enhance computing capabilities by offloading complicated tasks to nearby cloud server.To conserve energy as well as maintain quality of service,low time complexity algorithm is proposed to complete task offloading and server allocation.In this paper,a multi-user with multiple tasks and single server scenario is considered for small network,taking full account of factors including data size,bandwidth,channel state information.Furthermore,we consider a multi-server scenario for bigger network,where the influence of task priority is taken into consideration.To jointly minimize delay and energy cost,we propose a distributed unsupervised learning-based offloading framework for task offloading and server allocation.We exploit a memory pool to store input data and corresponding decisions as key-value pairs for model to learn to solve optimization problems.To further reduce time cost and achieve near-optimal performance,we use convolutional neural networks to process mass data based on fully connected networks.Numerical results show that the proposed algorithm performs better than other offloading schemes,which can generate near-optimal offloading decision timely.展开更多
OBJECTIVE: To study metabolic characteristics of fever in rats induced by 2, 4-dinitrophenol (DNP) and the effect of Huanglianjiedu Tang (HLJDT) on the fever. METHODS: The urine samples were analyzed by ultra-performa...OBJECTIVE: To study metabolic characteristics of fever in rats induced by 2, 4-dinitrophenol (DNP) and the effect of Huanglianjiedu Tang (HLJDT) on the fever. METHODS: The urine samples were analyzed by ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) at the positive ion mode scanning, and experimental data were analyzed by the principal component analysis. RESULTS: Eight potential biomarkers indicating the occurrence and evolvement of fever were determined according to ions in urine samples. Five of them were found increased, while the other three decreased. After HLJDD intervention, the increased five were reduced significantly in high dose group, compared with model group, while the decreased three had no obvious change. Five of the eight biomakers were identified with formyl-5-hy-droxykynurenamine, gentisic acid, aminoadipic acid, phenylacetic acid, L-phenylalanyl-L-hydroxyproline on the basis of MS/MS.These biomarkers are associated with the metabolism of 5-hydroxytryptamine, tyrosine, lysine, phenylalanine and collagen protein, respectively. CONCLUSION: HLJDT had significant effect on DNP-induced fever in rats. The effect was performed possibly by acting on 5-hydroxytryptamine in hypothalamus and some amino acid metabolism. These results suggested that HLJDT relieved fever by acting on multi-targets.展开更多
Apoptosis and viability of PC12 cells following 1-methyl-4-phenylpyridinium ion （MPP＋）-induced injury were monitored by flow cytometry, following Annexin V-propidium iodide double labeling, and 3-（4,5-Dimethylthia...Apoptosis and viability of PC12 cells following 1-methyl-4-phenylpyridinium ion （MPP＋）-induced injury were monitored by flow cytometry, following Annexin V-propidium iodide double labeling, and 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay, respectively. The release of lactate dehydrogenase, superoxide dismutase activity and levels of malondialdehyde were determined by UV spectrophotometry. The changes in mitochondrial membrane potential and the intracellular concentration of calcium were determined by flow cytometry, and the activity of caspase-3 was monitored by western blot. According to cell viability and apoptosis studies, MPP＋-induced apoptosis in PC12 cells was inhibited in the presence of 10 tJg/mL of Eleutheroside B Our results indicate that the neuroprotective effect of Eleutheroside B, following MPP＋-induced apoptosis in PC12 cells, involves increasing the anti-oxidative stress capacity of cells, maintaining the high-energy state of mitochondrial membrane potential, reducing intracellular calcium concentration and inhibiting caspase-3 activity.展开更多
With the increasing demand for energy storage technology, iron-chromium flow batteries(ICFBs) have been widely concerned because of their price advantage. However, the low electrochemical activity of Cr^(3+)/Cr^(2+)re...With the increasing demand for energy storage technology, iron-chromium flow batteries(ICFBs) have been widely concerned because of their price advantage. However, the low electrochemical activity of Cr^(3+)/Cr^(2+)redox couples and the side hydrogen evolution reaction limit the industrial application of ICFBs. A multi-dimensional Bi/carbon composite electrocatalyst(Bi@C)for ICFB is designed and prepared to improve the electrochemical activity of Cr^(3+)/Cr^(2+)redox couples. Benefiting by using the Bimetal organic framework(Bi-MOF) with solid matrix as precursors, Bi nanospheres are highly dispersed on the Bi@C electrocatalyst that effectively enhances the electrochemical activity. The special morphology of Bi@C electrocatalyst helps the transfer of electrons and ions, significantly reducing the polarization of battery. Herein, the 3D porous carbon frames accelerate mass transfer, and the 2D carbon nanobelts and carbon layer coating on 0D Bi nanospheres improve the conductivity of Bi nanospheres. Therefore, the ICFB with multi-dimensional Bi@C electrocatalyst exhibits coulombic efficiency of 98.10% and energy efficiency of 79.14% at 140 m A cm^(-2), which is higher than ICFBs with commercial graphite carbon electrocatalyst and with heat treatment carbon felt. This work provides a simple and economical method to fabricate a high-performance multidimensional Bi@C electrocatalyst for Cr^(3+)/Cr^(2+)redox couples, boosting the development of ICFBs.展开更多
Highly dispersed Pt-based single-atom alloys have been extensively studied in heterogeneous catalysis,particularly for propane dehydrogenation(PDH).However,Pt-based single-atom alloys still suffer from sintering and c...Highly dispersed Pt-based single-atom alloys have been extensively studied in heterogeneous catalysis,particularly for propane dehydrogenation(PDH).However,Pt-based single-atom alloys still suffer from sintering and coke deposition in high-temperature dehydrogenation reactions.Additionally,the atomic structure of the active sites of Pt-based single-atom alloys for PDH remains elusive,and the solid chemistry occurring on the catalyst surface is still under debate.In this work,we discovered that the coordination environment of the single Pt atom for Pt_(1)Cu_(30)cluster catalysts,encapsulated with a carbon layer,can be regulated by sequential heat treatment under air and H_(2)atmosphere.The Pt_(1)Cu_(30)cluster catalyst,with a single Pt atom coordinated by 9 Cu atoms,is similar to the surface structure of Pt_(1)Cu_(3)(111)and exhibits excellent catalytic activity and stability at high temperatures,maintaining propane conversion of 43.5%and propylene selectivity of 98.2%,with a deactivation constant(Kd)of 0.01 h^(−1),even after 32 h of testing at 600℃.The combination of structural characterizations and temperature programmed analysis reveal that the single Pt atom coordinated by around 9 Cu atoms of the Pt_(1)Cu_(30)cluster catalyst,serves as the vital active sites for C-H cleavage of propane and propylene desorption due to their electron-rich and geometrically isolated Pt atom structure.Furthermore,the thin carbon layer coated on the surface of Pt_(1)Cu_(30)clusters can effectively reduce the desorption energy of propylene,thereby avoiding further dehydrogenation and improving the propylene yield and catalytic stability.展开更多
基金supported by Heilongjiang Postdoctoral Program(ZHY2022-114)General program of Heilongjiang Province(LBHZ22251)Young Elite Scientists Sponsorship Program(2022-QNRC1-27)。
文摘Calycosin,Astragali Radix most prominent ingredient,has drawn more attention as a result of its ability to treat atherosclerosis(AS).However,the mechanism of action has not been fully elucidated.We investigated the effects of calycosin on bile acid(BA)metabolism and gut microbiome in ApoE^(-/-)mice fed a high-fat diet(HFD).The data showed that the aorta of ApoE^(-/-)mice treated with HFD showed significant atheromatous plaque formation and lipid accumulation,and the levels of total cholesterol(TC),triglycerides(TG)and low-density lipoprotein cholesterol(LDL-C)were significantly increased,while the levels of high-density lipoprotein cholesterol(HDL-C)were significantly decreased.Calycosin can substantially regulate lipid levels,thereby alleviating liver lipid deposition induced by atherosclerosis.In addition,16S rRNA sequencing showed that calycosin treatment has reshaped the gut microbiota disturbed by HFD,in particular,increasing the ratio of Bacteroidetes/Firmicutes,and improving the relative abundance of Bilophila,Desulfovibrio,Bacteroides,Lactobacillus,etc.Meanwhile,targeted metabolomics analysis showed that calycosin treatment significantly modulated glycodeoxycholic acid(CDCA),taurocholic acids(TCA),lithocholic acid(LCA),deoxycholic acid(DCA),taurodeoxycholic acid(TDCA)and BA pool composition,which were associated with atherosclerotic plaque areas.In addition,calycosin treatment also down-regulated farnesoid X receptor(FXR)protein levels and up-regulated cytochrome P450 family 7 subfamily A member 1(CYP7A1)protein levels in the hepatic.At the same time,calycosin inhibits the ileum FXR/TGR5 signaling pathway,inhibits BA reabsorption,promotes BA excretion,and reduces hepatic cholesterol accumulation by enterohepatic circulation.In addition,we found that calycosin significantly promoted the expression of hepatic ATP-binding cassette transporter A1(ABCA1)and ABCG1 to mediate cholesterol efflux.Meanwhile,calycosin regulates gut microbiota,and Bacteroides,Alistipes,Desulfovibrio,Lactobacillus,Bilophila and Odoribacter are closely related to specific BAs.This enables us to further understand the relationship between BA metabolism and gut microbiota.Calycosin may reduce high-fat diet-induced hepatic cholesterol accumulation in ApoE^(-/-)mice through gut microbiota and BA metabolism,and play a role in treating AS.Finally,we confirmed that calycosin-altered gut microbiota by fecal microbiota transplantation was sufficient to alleviate atherosclerosis.Taken together,our findings provide important insights into the pharmacological mechanisms underlying the efficacy of calycosin on atherosclerosis.
基金the Key Project of Technology of Ministry of Education, No. 2007029
文摘Gentianine has been shown to have a protective effect on hippocampal CA1 neurons in rats subjected to recurrent febrile convulsion（FC）.The present study sought to explore the possible mechanism of gentianine by intraperitoneally injecting gentianine into rats with warm water-induced FC.The results revealed that neuronal organelle injury was slightly ameliorated in the hippocampal CA1 region.The level of glutamate was decreased,but the level of γ-aminobutyric acid was increased,as detected by ninhydrin staining.In addition,glutamate acid decarboxylase expression in hippocampal CA1 was increased,as determined by immunohistochemistry.The results demonstrated that gentianine can ameliorate FC-induced neuronal injury by enhancing glutamate acid decarboxylase activity,decreasing glutamate levels and increasing γ-aminobutyric acid levels.
基金presented in part at the EAI CHINACOM 2020supported in part by Natural Science Foundation of Jiangxi Province (Grant No.20202BAB212003)+1 种基金Projects of Humanities and Social Sciences of universities in Jiangxi (JC18224)Science and technology project of Jiangxi Provincial Department of Education(GJJ210817, GJJ210854)
文摘Mobile edge computing has emerged as a new paradigm to enhance computing capabilities by offloading complicated tasks to nearby cloud server.To conserve energy as well as maintain quality of service,low time complexity algorithm is proposed to complete task offloading and server allocation.In this paper,a multi-user with multiple tasks and single server scenario is considered for small network,taking full account of factors including data size,bandwidth,channel state information.Furthermore,we consider a multi-server scenario for bigger network,where the influence of task priority is taken into consideration.To jointly minimize delay and energy cost,we propose a distributed unsupervised learning-based offloading framework for task offloading and server allocation.We exploit a memory pool to store input data and corresponding decisions as key-value pairs for model to learn to solve optimization problems.To further reduce time cost and achieve near-optimal performance,we use convolutional neural networks to process mass data based on fully connected networks.Numerical results show that the proposed algorithm performs better than other offloading schemes,which can generate near-optimal offloading decision timely.
基金Supported by the National Basic Research Program of China(No.2007CB512608)
文摘OBJECTIVE: To study metabolic characteristics of fever in rats induced by 2, 4-dinitrophenol (DNP) and the effect of Huanglianjiedu Tang (HLJDT) on the fever. METHODS: The urine samples were analyzed by ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry (UPLC/ESI-Q-TOF-MS) at the positive ion mode scanning, and experimental data were analyzed by the principal component analysis. RESULTS: Eight potential biomarkers indicating the occurrence and evolvement of fever were determined according to ions in urine samples. Five of them were found increased, while the other three decreased. After HLJDD intervention, the increased five were reduced significantly in high dose group, compared with model group, while the decreased three had no obvious change. Five of the eight biomakers were identified with formyl-5-hy-droxykynurenamine, gentisic acid, aminoadipic acid, phenylacetic acid, L-phenylalanyl-L-hydroxyproline on the basis of MS/MS.These biomarkers are associated with the metabolism of 5-hydroxytryptamine, tyrosine, lysine, phenylalanine and collagen protein, respectively. CONCLUSION: HLJDT had significant effect on DNP-induced fever in rats. The effect was performed possibly by acting on 5-hydroxytryptamine in hypothalamus and some amino acid metabolism. These results suggested that HLJDT relieved fever by acting on multi-targets.
基金the Major Projects of National Science and Technology, No.2009ZX09103-329the National Natural Science Foundation for Distinguished Young Scholars of China, No.30901974+1 种基金Outstanding Youth Science Fund Program of Heilongjiang Province, No.JC200705"Spring Sunshine" Plan of Ministry of Education, No.2006
文摘Apoptosis and viability of PC12 cells following 1-methyl-4-phenylpyridinium ion （MPP＋）-induced injury were monitored by flow cytometry, following Annexin V-propidium iodide double labeling, and 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay, respectively. The release of lactate dehydrogenase, superoxide dismutase activity and levels of malondialdehyde were determined by UV spectrophotometry. The changes in mitochondrial membrane potential and the intracellular concentration of calcium were determined by flow cytometry, and the activity of caspase-3 was monitored by western blot. According to cell viability and apoptosis studies, MPP＋-induced apoptosis in PC12 cells was inhibited in the presence of 10 tJg/mL of Eleutheroside B Our results indicate that the neuroprotective effect of Eleutheroside B, following MPP＋-induced apoptosis in PC12 cells, involves increasing the anti-oxidative stress capacity of cells, maintaining the high-energy state of mitochondrial membrane potential, reducing intracellular calcium concentration and inhibiting caspase-3 activity.
基金supported by the Industry-University-Research Cooperation project of Sinopec Dalian Petrochemical Research Institute (323038)the Fundamental Research Funds for the Central Universities (3132024625)the Young Technology Talents Fund of Dalian City (2022RQ027)。
文摘With the increasing demand for energy storage technology, iron-chromium flow batteries(ICFBs) have been widely concerned because of their price advantage. However, the low electrochemical activity of Cr^(3+)/Cr^(2+)redox couples and the side hydrogen evolution reaction limit the industrial application of ICFBs. A multi-dimensional Bi/carbon composite electrocatalyst(Bi@C)for ICFB is designed and prepared to improve the electrochemical activity of Cr^(3+)/Cr^(2+)redox couples. Benefiting by using the Bimetal organic framework(Bi-MOF) with solid matrix as precursors, Bi nanospheres are highly dispersed on the Bi@C electrocatalyst that effectively enhances the electrochemical activity. The special morphology of Bi@C electrocatalyst helps the transfer of electrons and ions, significantly reducing the polarization of battery. Herein, the 3D porous carbon frames accelerate mass transfer, and the 2D carbon nanobelts and carbon layer coating on 0D Bi nanospheres improve the conductivity of Bi nanospheres. Therefore, the ICFB with multi-dimensional Bi@C electrocatalyst exhibits coulombic efficiency of 98.10% and energy efficiency of 79.14% at 140 m A cm^(-2), which is higher than ICFBs with commercial graphite carbon electrocatalyst and with heat treatment carbon felt. This work provides a simple and economical method to fabricate a high-performance multidimensional Bi@C electrocatalyst for Cr^(3+)/Cr^(2+)redox couples, boosting the development of ICFBs.
基金the National Key Research and Development Program of China(No.2024YFC3908700)the National Natural Science Foundation of China(Nos.21971070 and 22176063)+5 种基金Guangdong Innovative and Entrepreneurial Research Team Program(No.2019ZT08L075)Guangdong Pearl River Talent Program(No.2019QN01L159)the Natural Science Foundation of Guangdong Province(No.2022A1515012047)Foshan Innovative and Entrepreneurial Research Team Program(No.2018IT100031)Yunnan Fundamental Research Projects(No.202401AU070180)Guangdong Basic and Applied Basic Research Foundation(No.2021A1515110427).
文摘Highly dispersed Pt-based single-atom alloys have been extensively studied in heterogeneous catalysis,particularly for propane dehydrogenation(PDH).However,Pt-based single-atom alloys still suffer from sintering and coke deposition in high-temperature dehydrogenation reactions.Additionally,the atomic structure of the active sites of Pt-based single-atom alloys for PDH remains elusive,and the solid chemistry occurring on the catalyst surface is still under debate.In this work,we discovered that the coordination environment of the single Pt atom for Pt_(1)Cu_(30)cluster catalysts,encapsulated with a carbon layer,can be regulated by sequential heat treatment under air and H_(2)atmosphere.The Pt_(1)Cu_(30)cluster catalyst,with a single Pt atom coordinated by 9 Cu atoms,is similar to the surface structure of Pt_(1)Cu_(3)(111)and exhibits excellent catalytic activity and stability at high temperatures,maintaining propane conversion of 43.5%and propylene selectivity of 98.2%,with a deactivation constant(Kd)of 0.01 h^(−1),even after 32 h of testing at 600℃.The combination of structural characterizations and temperature programmed analysis reveal that the single Pt atom coordinated by around 9 Cu atoms of the Pt_(1)Cu_(30)cluster catalyst,serves as the vital active sites for C-H cleavage of propane and propylene desorption due to their electron-rich and geometrically isolated Pt atom structure.Furthermore,the thin carbon layer coated on the surface of Pt_(1)Cu_(30)clusters can effectively reduce the desorption energy of propylene,thereby avoiding further dehydrogenation and improving the propylene yield and catalytic stability.
