Introduction:On March 20,2025,a suspected Zika virus(ZIKV)case departed Thailand and flew to Nanning Wuxu International Airport before transiting to Jinan.Upon receiving notification,local CDCs immediately initiated c...Introduction:On March 20,2025,a suspected Zika virus(ZIKV)case departed Thailand and flew to Nanning Wuxu International Airport before transiting to Jinan.Upon receiving notification,local CDCs immediately initiated comprehensive epidemiological investigations,laboratory testing,and preventive control measures.Methods:We collected urine,sputum,and blood samples from the patient for analysis.Quantitative real-time reverse-transcription polymerase chain reaction(qRT-PCR)was employed to detect ZIKV nucleic acid.Metagenome Next-Generation Sequencing(mNGS)was performed on the urine sample to obtain complete viral genome sequences.Phylogenetic analysis was subsequently constructed using the obtained sequences to determine the origin,genotype,and mutation profile of this imported case.Results:The qRT-PCR analysis confirmed ZIKV presence in the patient’s urine,sputum,and serum samples.The mNGS successfully generated the complete ZIKV genome sequence.Phylogenetic analysis demonstrated that the ZIKV strain belonged to the Asian lineage,exhibiting 99.57%nucleotide homology with a ZIKV strain from Bangkok,Thailand(GenBank accession no.OR264645.1).Conclusion:Based on the patient’s epidemiological history,clinical presentation,and nucleic acid test results from multiple specimens,this case was confirmed as the first imported ZIKV infection documented in Shandong Province,with the infection source traced to Thailand.展开更多
As of August 15,2021,2 confirmed cases of anthrax were reported to Shandong CDC,one of which was a 14-year-old student(Patient A)and the other was a 35-year-old man(Patient B)engaging in the slaughter of cattle.Patien...As of August 15,2021,2 confirmed cases of anthrax were reported to Shandong CDC,one of which was a 14-year-old student(Patient A)and the other was a 35-year-old man(Patient B)engaging in the slaughter of cattle.Patient A died on August 6,and Patient B has been transferred to an infectious disease hospital for isolation and treatment.The Shandong CDC formed a joint investigation team to determine the source of infection and prevent any further spread.展开更多
Introduction:Rabies is a viral zoonotic disease that causes progressive and fatal inflammation in the brain.Rabies has caused more than 5,000 human deaths in Shandong Province since 1955.This study aimed to analyze th...Introduction:Rabies is a viral zoonotic disease that causes progressive and fatal inflammation in the brain.Rabies has caused more than 5,000 human deaths in Shandong Province since 1955.This study aimed to analyze the epidemiological characteristics of human rabies in Shandong Province from 2010 to 2020 and to provide a scientific basis for policy changes.Methods:The data of reported human rabies cases from 2010 to 2020 were obtained from China’s National Notifiable Disease Reporting System,and data related to exposure and post-exposure prophylaxis(PEP)of the cases were acquired through case investigation.Results:A total of 414 human rabies cases were reported in Shandong Province from 2010 to 2020.Out of the 414 total cases,87.20%were primarily farmers;83.10%were over 40 years old.70.29%(265/377)belonged to categoryⅢexposure;and 96.67%(377/390)were exposed to the virus through infected dogs.The vaccine inoculation rate of these cases after exposure was only 8.85%;1.03%(4/390)had been vaccinated with rabies immunoglobulin,developing the disease 11 to 13 days after categoryⅢexposure.Conclusions:Dogs were still the primary animal hosts.Most rabies patients died due to no or inadequate post-exposure prophylaxis.Vaccine inoculation rates for dogs should reach the target of 70%as soon as possible.Health departments should improve the accessibility and quality of PEP;and improve the health literacy of the elderly in rural areas.展开更多
What is already known about this topic?Brucellosis,mainly caused by Brucella melitensis(B.melitensis),is regarded as a significant zoonotic disease in China.In Weihai,located at the eastern end of the Shandong Peninsu...What is already known about this topic?Brucellosis,mainly caused by Brucella melitensis(B.melitensis),is regarded as a significant zoonotic disease in China.In Weihai,located at the eastern end of the Shandong Peninsula,brucellosis has been in a low epidemic phase for the past five years.What is added by this report?This was the initial report of a brucellosis outbreak in the last five years.Strains of B.melitensis bv.3 from Weihai and other cities showed a close genetic relationship,suggesting a potential common ancestry.What are the implications for public health practice?Epidemiological investigations depend on standardized and effective molecular typing methods and analysis tools for public health laboratories to identify and trace outbreaks.Understanding the circulation patterns of livestock in free-range households in heavily affected areas is essential for controlling the spread of brucellosis.展开更多
文摘Introduction:On March 20,2025,a suspected Zika virus(ZIKV)case departed Thailand and flew to Nanning Wuxu International Airport before transiting to Jinan.Upon receiving notification,local CDCs immediately initiated comprehensive epidemiological investigations,laboratory testing,and preventive control measures.Methods:We collected urine,sputum,and blood samples from the patient for analysis.Quantitative real-time reverse-transcription polymerase chain reaction(qRT-PCR)was employed to detect ZIKV nucleic acid.Metagenome Next-Generation Sequencing(mNGS)was performed on the urine sample to obtain complete viral genome sequences.Phylogenetic analysis was subsequently constructed using the obtained sequences to determine the origin,genotype,and mutation profile of this imported case.Results:The qRT-PCR analysis confirmed ZIKV presence in the patient’s urine,sputum,and serum samples.The mNGS successfully generated the complete ZIKV genome sequence.Phylogenetic analysis demonstrated that the ZIKV strain belonged to the Asian lineage,exhibiting 99.57%nucleotide homology with a ZIKV strain from Bangkok,Thailand(GenBank accession no.OR264645.1).Conclusion:Based on the patient’s epidemiological history,clinical presentation,and nucleic acid test results from multiple specimens,this case was confirmed as the first imported ZIKV infection documented in Shandong Province,with the infection source traced to Thailand.
文摘As of August 15,2021,2 confirmed cases of anthrax were reported to Shandong CDC,one of which was a 14-year-old student(Patient A)and the other was a 35-year-old man(Patient B)engaging in the slaughter of cattle.Patient A died on August 6,and Patient B has been transferred to an infectious disease hospital for isolation and treatment.The Shandong CDC formed a joint investigation team to determine the source of infection and prevent any further spread.
文摘Introduction:Rabies is a viral zoonotic disease that causes progressive and fatal inflammation in the brain.Rabies has caused more than 5,000 human deaths in Shandong Province since 1955.This study aimed to analyze the epidemiological characteristics of human rabies in Shandong Province from 2010 to 2020 and to provide a scientific basis for policy changes.Methods:The data of reported human rabies cases from 2010 to 2020 were obtained from China’s National Notifiable Disease Reporting System,and data related to exposure and post-exposure prophylaxis(PEP)of the cases were acquired through case investigation.Results:A total of 414 human rabies cases were reported in Shandong Province from 2010 to 2020.Out of the 414 total cases,87.20%were primarily farmers;83.10%were over 40 years old.70.29%(265/377)belonged to categoryⅢexposure;and 96.67%(377/390)were exposed to the virus through infected dogs.The vaccine inoculation rate of these cases after exposure was only 8.85%;1.03%(4/390)had been vaccinated with rabies immunoglobulin,developing the disease 11 to 13 days after categoryⅢexposure.Conclusions:Dogs were still the primary animal hosts.Most rabies patients died due to no or inadequate post-exposure prophylaxis.Vaccine inoculation rates for dogs should reach the target of 70%as soon as possible.Health departments should improve the accessibility and quality of PEP;and improve the health literacy of the elderly in rural areas.
基金Supported by Medical and Health Development Plan of Shandong(2015WS0272)TCM science and technology project of Shandong(Q-2022091).
文摘What is already known about this topic?Brucellosis,mainly caused by Brucella melitensis(B.melitensis),is regarded as a significant zoonotic disease in China.In Weihai,located at the eastern end of the Shandong Peninsula,brucellosis has been in a low epidemic phase for the past five years.What is added by this report?This was the initial report of a brucellosis outbreak in the last five years.Strains of B.melitensis bv.3 from Weihai and other cities showed a close genetic relationship,suggesting a potential common ancestry.What are the implications for public health practice?Epidemiological investigations depend on standardized and effective molecular typing methods and analysis tools for public health laboratories to identify and trace outbreaks.Understanding the circulation patterns of livestock in free-range households in heavily affected areas is essential for controlling the spread of brucellosis.
