The prefrontal cortex(PFC)plays a pivotal role in orchestrating higher-order emotional and cognitive processes,a function that depends on the precise modulation of synaptic activity.Although pharmacological studies ha...The prefrontal cortex(PFC)plays a pivotal role in orchestrating higher-order emotional and cognitive processes,a function that depends on the precise modulation of synaptic activity.Although pharmacological studies have demonstrated that dopamine signaling through dopamine D1 receptor(DRD1)in the PFC is essential for these functions,the cell-type-specific and molecular mechanisms underlying the neuromodulatory effects remain elusive.Using cell-type-specific knockout mice and patch-clamp recordings,we investigated the regulatory role of DRD1 on neurons and astrocytes in synaptic transmission and plasticity.Furthermore,we explored the mechanisms by which DRD1 on astrocytes regulate synaptic transmission and plasticity at the cellular level,as well as emotional and cognitive functions at the behavioral level,through two-photon imaging,microdialysis,high-performance liquid chromatography,transcriptome sequencing,and behavioral testing.We found that conditional knockout of the Drd1 in astrocytes(CKO^(AST))increased glutamatergic synaptic transmission and longterm potentiation(LTP)in the medial prefrontal cortex(mPFC),whereas Drd1 deletion in pyramidal neurons did not affect synaptic transmission.The elevated level of D-serine in the mPFC of CKO^(AST)mice increased glutamatergic transmission and LTP through NMDA receptors.In addition,CKO^(AST)mice exhibited abnormal emotional and cognitive function.Notably,these behavioral changes in CKO^(AST)mice could be reversed through the administration of D-serine degrease to the mPFC.These results highlight the critical role of the astrocytic DRD1 in modulating mPFC synaptic transmission and plasticity,as well as higher brain functions through D-serine,and may shed light on the treatment of mental disorders.展开更多
基金supported by grants from the National Key R&D Program of China(2022ZD0204700,2021ZD0202700,2022ZD0214300)National Natural Science Foundation of China(T2394535,82090032,32300968,and 32271014)+1 种基金Guangdong-Hong Kong Joint Laboratory for Psychiatric Disorders(2023B1212120004,China)the Guangdong Basic and Applied Basic Research Foundation(2020A1515110565,China).
文摘The prefrontal cortex(PFC)plays a pivotal role in orchestrating higher-order emotional and cognitive processes,a function that depends on the precise modulation of synaptic activity.Although pharmacological studies have demonstrated that dopamine signaling through dopamine D1 receptor(DRD1)in the PFC is essential for these functions,the cell-type-specific and molecular mechanisms underlying the neuromodulatory effects remain elusive.Using cell-type-specific knockout mice and patch-clamp recordings,we investigated the regulatory role of DRD1 on neurons and astrocytes in synaptic transmission and plasticity.Furthermore,we explored the mechanisms by which DRD1 on astrocytes regulate synaptic transmission and plasticity at the cellular level,as well as emotional and cognitive functions at the behavioral level,through two-photon imaging,microdialysis,high-performance liquid chromatography,transcriptome sequencing,and behavioral testing.We found that conditional knockout of the Drd1 in astrocytes(CKO^(AST))increased glutamatergic synaptic transmission and longterm potentiation(LTP)in the medial prefrontal cortex(mPFC),whereas Drd1 deletion in pyramidal neurons did not affect synaptic transmission.The elevated level of D-serine in the mPFC of CKO^(AST)mice increased glutamatergic transmission and LTP through NMDA receptors.In addition,CKO^(AST)mice exhibited abnormal emotional and cognitive function.Notably,these behavioral changes in CKO^(AST)mice could be reversed through the administration of D-serine degrease to the mPFC.These results highlight the critical role of the astrocytic DRD1 in modulating mPFC synaptic transmission and plasticity,as well as higher brain functions through D-serine,and may shed light on the treatment of mental disorders.
