In the wave of the“Internet+AI”era,information technology is comprehensively reshaping the landscape of college English reading education.Traditional teaching models struggle to meet the demands of talent cultivatio...In the wave of the“Internet+AI”era,information technology is comprehensively reshaping the landscape of college English reading education.Traditional teaching models struggle to meet the demands of talent cultivation in the new era.The integration of“Internet+AI”technologies brings revolutionary opportunities to reading instruction,significantly enriching teaching resources,enabling personalized teaching,enhancing interactivity,and optimizing evaluation systems.Guided by principles such as student-centeredness and integrated innovation,this study proposes multiple strategies for advancing teaching practices.Using the Understanding Contemporary China:English Reading and Writing Tutorial(Foreign Language Teaching and Research Press)as a case study,this paper explores practical pathways for reforming college English reading instruction,aiming to improve teaching quality and students’comprehensive English reading literacy.展开更多
Varicella-zoster virus(VZV)belongs to a neurotropic Alphaherpesvirinae and is the causative pathogen of both the varicella and herpes zoster.Currently,there are three different vaccines for the prevention,low does liv...Varicella-zoster virus(VZV)belongs to a neurotropic Alphaherpesvirinae and is the causative pathogen of both the varicella and herpes zoster.Currently,there are three different vaccines for the prevention,low does live-attenuated Oka(v Oka)against varicella,high does v Oka and glycoprotein E with the AS01B adjuvant system against zoster.Varicella vaccine containing v Oka effectively prevents varicella,but latency of v Oka can be detected in the inoculated population and its reactivation causes zoster.展开更多
Bacterial infection of wounds is an escalating medical problem,issuing threats to both global public health and personal health.Photothermal antibacterial technology as a novel sterilization strategy has outstanding s...Bacterial infection of wounds is an escalating medical problem,issuing threats to both global public health and personal health.Photothermal antibacterial technology as a novel sterilization strategy has outstanding sterilization efficiency,high safety and low risk of emergence of drug-resistant bacteria.By combining inherent antibacterial activity and light-assisted antibacterial treatment,developing novel multifunctional dressings with synergistic high-efficiency antibacterial effects and also promoting wound healing possesses attractive advantages in the field of treating bacterial wound infections in clinical care.Herein,a multifunctional hydrogel formed by in situ photo-cross linking was designed and prepared by first grafting methacrylic anhydride as a photosensitizer onto chitosan,and then introducing oxidatively synthesized polydopamine(PDA).The physicochemical characterizations of the synthesized hydrogels demonstrated their tunability certainly associated with PDA concentration,including pore size,water swelling,rheological properties and in vitro degradability.In addition,the composite hydrogels exhibited good adhesion,anti-oxidation and photothermal properties due to the existence of PDA.Within 10min upon exposure to 808 nm near-infrared(NIR)light irradiation,this hydrogel system displayed outstanding antibacterial activity against Staphylococcus aureus with almost 100%killing efficiency,of which rapid efficient sterilization plays a significant role in wound healing.Moreover,the hydrogel is capable of cytocompatibility and has low toxicity to murine fibroblasts(L929 and NIH/3T3).In the full-thickness wound defect infection model in mice,the wound closure ratio,inflammatory response,fibroblasts,neovascularization and epithelialization were measured.Animal experiments also reveal that the hydrogel assisted with NIR laser irradiation can inhibit effectively infection at an early stage and accelerate the wound healing process.In summary,this novel multifunctional injectable hydrogel exhibits excellent swelling capacity,bio-adhesion,antioxidant property,photothermal activity,efficient antibacterial property and facilitates skin healing,which has great promising application as a medical dressing biomaterial in infected wound carefields.展开更多
MicroRNAs( miRNAs) are reported to be associated with various diseases. The identification of disease-related miRNAs would be beneficial to the disease diagnosis and prognosis. However,in contrast with the widely avai...MicroRNAs( miRNAs) are reported to be associated with various diseases. The identification of disease-related miRNAs would be beneficial to the disease diagnosis and prognosis. However,in contrast with the widely available expression profiling, the limited knowledge of molecular function restrict the development of previous methods based on network similarity measure. To construct reliable training data,the decision fusion method is used to prioritize the results of existing methods. After that,the performance of decision fusion method is validated. Furthermore,in consideration of the long range dependencies of successive expression values,Hidden Conditional Random Field model( HCRF) is selected and applied to miRNA expression profiling to infer disease-associated miRNAs. The results show that HCRF achieves superior performance and outperforms the previous methods. The results also demonstrate the power of using expression profiling for discovering disease-associated miRNAs.展开更多
Congenital human cytomegalovirus(HCMV) infection is a leading infectious cause of birth defects.Previous studies have reported birth defects with multiple organ maldevelopment in congenital HCMV-infected neonates. Mul...Congenital human cytomegalovirus(HCMV) infection is a leading infectious cause of birth defects.Previous studies have reported birth defects with multiple organ maldevelopment in congenital HCMV-infected neonates. Multipotent mesenchymal stromal cells(MSCs) are a group of stem/progenitor cells that are multi-potent and can self-renew, and they play a vital role in multiorgan formation. Whether MSCs are susceptible to HCMV infection is unclear. In this study, MSCs were isolated from Wharton's jelly of the human umbilical cord and identified by their plastic adherence, surface marker pattern, and differentiation capacity. Then, the MSCs were infected with the HCMV Towne strain, and infection status was assessed via determination of viral entry,replication initiation, viral protein expression, and infectious virion release using western blotting,immunofluorescence assays, and plaque forming assays. The results indicate that the isolated MSCs were fully permissive for HCMV infection and provide a preliminary basis for understanding the pathogenesis of HCMV infection in non-nervous system diseases, including multi-organ malformation during fetal development.展开更多
This paper introduced the application of the WebGIS and virtual reality technology in the tourism industry and analyzed the existing problems in the tourism information system.Taking Daqing Oil Field Scenic Sport as a...This paper introduced the application of the WebGIS and virtual reality technology in the tourism industry and analyzed the existing problems in the tourism information system.Taking Daqing Oil Field Scenic Sport as an example,it introduced a method of constructing the virtual simulation platform for tourism based on the combination of WebGIS and virtual reality technology.Through the virtual simulation platform of Daqing Tourism Scenic Spot,it verified the design idea,methods,and technical feasibility of the virtual tourism system,and built a specific,feasible,and practical virtual simulation platform for tourism.It showed the bright prospects of virtual tourism development based on WebGIS and virtual reality technology.展开更多
Biaxia lrotary tillage in dryland(DBRT)can complete biaxial rotary tillage with straw incorporation,secondary suppression,and ditching,and it has been previously studied in direct-seeded rice and wheat.However,the eff...Biaxia lrotary tillage in dryland(DBRT)can complete biaxial rotary tillage with straw incorporation,secondary suppression,and ditching,and it has been previously studied in direct-seeded rice and wheat.However,the effects of DBRT on the mechanically transplanted rice yield and greenhouse gas emissions remain unclear.To evaluate the effects of DBRT on improving the food security of mechanically transplanted rice and reducing the greenhouse gas emissions,we conducted an experiment for two years with wheat straw incorporation.Three tillage methods were set up:DBRT,uniaxial rotary tillage in dryland and paddy(DPURT),and uniaxial rotary tillage in paddy(PURT).The results showed that compared with DPURT and PURT,DBRT increased the yield of machine-transplanted rice by 7.5-11.0%and 13.3-26.7%,respectively,while the seasonal cumulative CH_(4) emissions were reduced by 13.9-21.2%and 30.2-37.0%,respectively,and the seasonal cumulative N_(2)O emissions were increased by 13.5-28.6%and 50.0-73.1%,respectively.Consequently,DBRT reduced the global warming potential by 10.7-15.5%and 23.7-28.6%,respectively,andtheyield-scaledglobalwarmingpotentialby18.2-21.8%and36.4-39.3%,respectively,compared to DPURT and PURT.These results were mainly related to the fact that DBRT significantly reduced soil bulk density and increased soil redox potential(Eh).Therefore,implementing DBRT in machine-transplanted rice fields is feasible,which cannot only increase the rice yield,but also reduce the greenhouse gas emissions.展开更多
Electrochemical heterogeneous catalytic ozonation(E-catazone)is a promising and advanced oxidation technology that uses a titanium dioxide nanoflower(TiO_(2-NF))-coated porous Ti gas diffuser as an anode material.Our ...Electrochemical heterogeneous catalytic ozonation(E-catazone)is a promising and advanced oxidation technology that uses a titanium dioxide nanoflower(TiO_(2-NF))-coated porous Ti gas diffuser as an anode material.Our previous study has highlighted that the importance of the TiO_(2-NF)coating layer in enhancing*OH production and rapidly degrading O_(3)-resistant drugs.It is well known that the properties of TiO_(2-NF)are closely related to its sintering temperature.However,to date,related research has not been conducted in E-catazone systems.Thus,this study evaluated the effect of the sintering temperature on the degradation of the O_(3)-resistant drug para-chlorobenzoic acid(p-CBA)using both experimental and kinetic modeling and revealed its influence mechanism.The results indicated that the TiO_(2-NF)sintering temperature could influence p-CBA degradation and*OH production.TiO_(2-NF)prepared at 450 C showcased the highest p-CBA removal efficiency(98.5%in 5 min)at a rate of 0.82 min^(-1),and an*OH exposure of 8.41 x 10^(-10)mol L^(-1)s.Kinetic modeling results and interface characterization data revealed that the sintering temperature could alter the TiO_(2)crystallized phase and the content of surfaceadsorbed oxygen,thus affecting the two key limiting reactions in the E-catazone process.That is,≡TiO_(2)surface reacted with H_(2)O to form TiO_(2)-(OH)_(2),which then heterogeneously catalyzed O_(3)to form*OH.Consequently,E-catazone with a TiO_(2-NF)anode prepared at 450°C generated the highest surface reaction rate(5.00 x 10^(-1)s^(-1)and 4.00 x 10^(-3)L mol^(-1)s^(-1),respectively),owing to its higher anatase content and adsorbed oxygen.Thus,a rapid O_(3)-TiO_(2)reaction was achieved,resulting in an enhanced*OH formation and a highly effective p-CBA degradation.Overall,this study provides novel baseline data to improve the application of E-catazone technology.展开更多
Bovine tuberculosis(bTB)is a chronic zoonotic disease that is endemic in China.Current in-vitro tests for bTB are mainly based on blood assays.Collection of samples results in some stress to the sampled cattle and ass...Bovine tuberculosis(bTB)is a chronic zoonotic disease that is endemic in China.Current in-vitro tests for bTB are mainly based on blood assays.Collection of samples results in some stress to the sampled cattle and associated economic losses for the herd owner.This study was designed to investigate the relationship between milk and serum antibody tests for bTB in dairy cows using 85 cows with milk and corresponding blood samples.Totally 4,395 milk samples were used to assesse the apparent(test)prevalence and incidence of bTB using the milk antibody ELISA.The association between levels of bTB milk antibody and milk quality was also evaluated.Milk and serum antibody tests showed a good correlation with a 87.5%(95%CI:61.7%,98.4)positive agreement and 98.7%(95%CI:95.4,99.8)negative agreement.The animal level lactoprevalence ranged from 0.3%(95%CI:0,1.2)to 33.3%(95%CI:26.6,40.6)in different farms and the incidence rate ranged from 0 head/cow-month(95%CI:0,0.02)to 0.04 head/cow-month(95%CI:0.02,0.07).Twenty percent of sampled farms met the criteria for bTB control in China.The prevalence on large-scale farms was lower(p<0.001)than on small farms.The bTB milk antibody levels had a negative correlation with milk yield and a positive correlation with somatic cell count(SCC),milk protein percentage(MPP)and percentage of total solids(TS).According to this research,milk ELISA could be used as a supplement of blood samples to assist in the surveillance for bTB and for alerting control and eradication of bTB.展开更多
Decarbonisation of power systems is essential for realising carbon neutrality,in which the economic cost caused by carbon needs to be qualified.Based on the formulation of locational marginal price(LMP),this paper pro...Decarbonisation of power systems is essential for realising carbon neutrality,in which the economic cost caused by carbon needs to be qualified.Based on the formulation of locational marginal price(LMP),this paper proposes a locational marginal electricity-carbon price(EC-LMP)model to reveal carbon-related costs caused by power consumers.A carbon-priceintegrated optimal power flow(C-OPF)is then developed to maximise economic efficiency of the power system considering the costs of electricity and carbon.Case studies are presented to demonstrate the new formulation and results demonstrate the efficacy of the EC-LMP-based C-OPF on decarbonisation and economy.展开更多
Perovskite solar cells(pero-SCs)performance is essentially limited by severe non-radiative losses and ion migration.Although numerous strategies have been proposed,challenges remain in the basic understanding of their...Perovskite solar cells(pero-SCs)performance is essentially limited by severe non-radiative losses and ion migration.Although numerous strategies have been proposed,challenges remain in the basic understanding of their origins.Here,we report a dielectric-screening-enhancement effect for perovskite defects by using organic semiconductors with finely tuned molecular structures from the atoms level.Our method produced various perovskite films with high dielectric constant values,reduced charge capture regions,suppressed ion migration,and it provides an efficient charge transport pathway for suppressing non-radiative recombination beyond the passivation effect.The resulting pero-SCs showed a promising power conversion efficiency(PCE)of 23.35%with a high open-circuit voltage(1.22 V);and the 1-cm^(2) pero-SCs maintained an excellent PCE(21.93%),showing feasibility for scalable fabrication.The robust operational and thermal stabilities revealed that this method paved a new way to understand the degradation mechanism of pero-SCs,promoting the efficiency,stability and scaled fabrication of the pero-SCs.展开更多
Increasing soil salinization has led to severe reductions in plant yield and quality,and investigating the mo-lecular mechanism of salt stress response is therefore an urgent priority.In this study,we systematically a...Increasing soil salinization has led to severe reductions in plant yield and quality,and investigating the mo-lecular mechanism of salt stress response is therefore an urgent priority.In this study,we systematically analyzed the response of cotton roots to salt stress using single-cell transcriptomics technology;56281 high-quality cells were obtained from 5-day-old lateral root tips of Gossypium arboreum under natural growth conditions and different salt treatments.Ten cell types with an array of novel marker genes were identified and confirmed by in situ RNA hybridization,and pseudotime analysis of some specific cell types revealed their potential differentiation trajectories.Prominent changes in cell numbers under salt stress were observed for outer epidermal and inner endodermal cells,which were significantly enriched in response to stress,amide biosynthetic process,glutathione metabolism,and glycolysis/gluconeogenesis.Analysis of differentially expressed genes identified in multiple comparisons revealed other functional ag-gregations concentrated on plant-type primary cell wall biogenesis,defense response,phenylpropanoid biosynthesis,and metabolic pathways.Some candidate differentially expressed genes encoding transcrip-tion factors or associated with plant hormones also responsive to salt stress were identified,and the func-tion of Ga03G2153,annotated as auxin-responsive GH3.6,was confirmed by virus-induced gene silencing.The GaGH3.6-silenced plants showed a severe stress-susceptible phenotype,and physiological and biochemical measurements indicated that they suffered more significant oxidative damage.These results suggest that GaGH3.6 might participate in cotton salt tolerance by regulating redox processes.We thus construct a transcriptional atlas of salt-stressed cotton roots at single-cell resolution,enabling us to explore cellular heterogeneity and differentiation trajectories and providing valuable insights into the mo-lecular mechanisms that underlie plant stress tolerance.展开更多
Aqueous zinc battery has been regarded as one of the most promising energy storage systems due to its low cost and environmental benignity.However,the safety concern on Zn anodes caused by uncontrolled Zn dendrite gro...Aqueous zinc battery has been regarded as one of the most promising energy storage systems due to its low cost and environmental benignity.However,the safety concern on Zn anodes caused by uncontrolled Zn dendrite growth in aqueous electrolyte hinders their application.Herein,sucrose with multi-hydroxyl groups has been introduced into aqueous electrolyte to modify Zn^(2+)solvation environment and create a protection layer on Zn anode,thus effectively retarding the growth of zinc dendrites.Atomistic simulations and experiments confirm that sucrose molecules can enter into the solvation sheath of Zn^(2+),and the as-formed unique solvation structure enhances the mobility of Zn^(2+).Such fast Zn^(2+)kinetics in sucrose-modified electrolyte can successfully suppress the dendrite growth.With this sucrose-modified aqueous electrolyte,Zn/Zn symmetric cells present more stable cycle performance than those using pure aqueous electrolyte;Zn/C cells also deliver an impressive higher energy density of 129.7 Wh·kg^(−1)and improved stability,suggesting a great potential application of sucrose-modified electrolytes for future Zn batteries.展开更多
An aligned composite film was fabricated via the deposition of carboxylic graphe ne oxide(C-GO)and polypyrrole(PPy)nano particles on alig ned poly(L-lactic acid)(PLLA)fiber-films(named as C-GO/PPy/PLLA),which has the ...An aligned composite film was fabricated via the deposition of carboxylic graphe ne oxide(C-GO)and polypyrrole(PPy)nano particles on alig ned poly(L-lactic acid)(PLLA)fiber-films(named as C-GO/PPy/PLLA),which has the core(PLLA)-sheath(C-GO/PPy)structure,and the composition of C-GO(~4.8 wt.%of PPy sheath)significantly enhanced the tensile strength and the conductivity of the PPy/PLLA film?Especially,after 4 weeks of immersion in the PBS solution,the conductivity and the tensile strength of CGO/PPy/PLLA films still remained-6.10 S/cm and 28.9 MPa,respectively,which could meet the need of the sustained electrical stimulation(ES)therapy for nerve repair.Moreover,the neurite length and the neurite alignment were significantly in creased through exerting ES on C-GO/PPy/PLLA films due to their sustained conductivity in the fluid of cell culture.These results indicated that C-GO/PPy/PLLA with sustained conductivity and mechanical property possessed great potential of nerve repair by exerting lasting-ES.展开更多
A sticky-flare gold nanoparticle probe(AuNP-probe)is designed by the combination of locked nucleic acid functionalized silencing of microRNA technology for intracellular microRNA-21(miRNA-21)sensitively detecting,fluo...A sticky-flare gold nanoparticle probe(AuNP-probe)is designed by the combination of locked nucleic acid functionalized silencing of microRNA technology for intracellular microRNA-21(miRNA-21)sensitively detecting,fluorescence imaging,localizing and silencing.The limit of detection is as low as 0.01 n M.Overexpressed miRNA-21 in cancer cells serves as endogenous drug release stimuli to trigger the release of probe-loaded doxorubicin(Dox),which soon translocates into cell nuclei.This multifunctional Dox-loaded AuNP-probe(Dox-AuNP-probe)could induce cancer cell apoptosis effectively through the synergistic effect of gene silencing and chemotherapy.This Dox-AuNP-probe exhibits superior drug potency compared to free Dox molecules,with a cell inhibition rate of 57%(but only 20%for Dox)to wild-type cancer cells and 30%(but 0%for Dox)to drug-resistent cancer cells after 72 h,and this strategy not only has the function of sensing,but also can effectively bypass drug resistance.In MCF-7 xenograft tumor-bearing mice,the Dox-AuNP-probes show greater inhibition for tumor tissues than miRNA-21 targeted AuNP-probes(Targeting-AuNP-probe)or free Dox molecules.Therefore,the Dox-AuNP-probe represents a promising nanotheranostic platform for future applications in cancer molecular imaging and therapy,especially providing a potential strategy to treat resistant cancers.展开更多
In the original publication the email addresses of corresponding authors have not been displayed.The correct email addresses of corresponding authors are provided in this correction.Fang-Cheng Li(sjwklfc@126.com),Fei ...In the original publication the email addresses of corresponding authors have not been displayed.The correct email addresses of corresponding authors are provided in this correction.Fang-Cheng Li(sjwklfc@126.com),Fei Hu(neuron111@163.com),Min-Hua Luo(luomh@wh.iov.cn).展开更多
Dear Editor,Gliomas are the most common brain tumors in adults which encompass all primary central nervous system(CNS)tumors of glial cell origin.The World Health Organization(WHO)classifies gliomas into four grades b...Dear Editor,Gliomas are the most common brain tumors in adults which encompass all primary central nervous system(CNS)tumors of glial cell origin.The World Health Organization(WHO)classifies gliomas into four grades based on the histologic/prognostic features.Because of the unclear etiol-ogy and pathogenesis,therapeutic efficacy and prognosis is poor.展开更多
文摘In the wave of the“Internet+AI”era,information technology is comprehensively reshaping the landscape of college English reading education.Traditional teaching models struggle to meet the demands of talent cultivation in the new era.The integration of“Internet+AI”technologies brings revolutionary opportunities to reading instruction,significantly enriching teaching resources,enabling personalized teaching,enhancing interactivity,and optimizing evaluation systems.Guided by principles such as student-centeredness and integrated innovation,this study proposes multiple strategies for advancing teaching practices.Using the Understanding Contemporary China:English Reading and Writing Tutorial(Foreign Language Teaching and Research Press)as a case study,this paper explores practical pathways for reforming college English reading instruction,aiming to improve teaching quality and students’comprehensive English reading literacy.
文摘Varicella-zoster virus(VZV)belongs to a neurotropic Alphaherpesvirinae and is the causative pathogen of both the varicella and herpes zoster.Currently,there are three different vaccines for the prevention,low does live-attenuated Oka(v Oka)against varicella,high does v Oka and glycoprotein E with the AS01B adjuvant system against zoster.Varicella vaccine containing v Oka effectively prevents varicella,but latency of v Oka can be detected in the inoculated population and its reactivation causes zoster.
基金funded by the National Natural Science Foundation of China(Nos.U21A20417 and 31930067)1.3.5 project for disciplines of excellence,West China Hospital,Sichuan University(No.ZYGD18002).
文摘Bacterial infection of wounds is an escalating medical problem,issuing threats to both global public health and personal health.Photothermal antibacterial technology as a novel sterilization strategy has outstanding sterilization efficiency,high safety and low risk of emergence of drug-resistant bacteria.By combining inherent antibacterial activity and light-assisted antibacterial treatment,developing novel multifunctional dressings with synergistic high-efficiency antibacterial effects and also promoting wound healing possesses attractive advantages in the field of treating bacterial wound infections in clinical care.Herein,a multifunctional hydrogel formed by in situ photo-cross linking was designed and prepared by first grafting methacrylic anhydride as a photosensitizer onto chitosan,and then introducing oxidatively synthesized polydopamine(PDA).The physicochemical characterizations of the synthesized hydrogels demonstrated their tunability certainly associated with PDA concentration,including pore size,water swelling,rheological properties and in vitro degradability.In addition,the composite hydrogels exhibited good adhesion,anti-oxidation and photothermal properties due to the existence of PDA.Within 10min upon exposure to 808 nm near-infrared(NIR)light irradiation,this hydrogel system displayed outstanding antibacterial activity against Staphylococcus aureus with almost 100%killing efficiency,of which rapid efficient sterilization plays a significant role in wound healing.Moreover,the hydrogel is capable of cytocompatibility and has low toxicity to murine fibroblasts(L929 and NIH/3T3).In the full-thickness wound defect infection model in mice,the wound closure ratio,inflammatory response,fibroblasts,neovascularization and epithelialization were measured.Animal experiments also reveal that the hydrogel assisted with NIR laser irradiation can inhibit effectively infection at an early stage and accelerate the wound healing process.In summary,this novel multifunctional injectable hydrogel exhibits excellent swelling capacity,bio-adhesion,antioxidant property,photothermal activity,efficient antibacterial property and facilitates skin healing,which has great promising application as a medical dressing biomaterial in infected wound carefields.
基金Sponsored by the National Natural Science Foundation of China(Grant Nos.61271346,61571163,61532014,61402132 and 91335112)
文摘MicroRNAs( miRNAs) are reported to be associated with various diseases. The identification of disease-related miRNAs would be beneficial to the disease diagnosis and prognosis. However,in contrast with the widely available expression profiling, the limited knowledge of molecular function restrict the development of previous methods based on network similarity measure. To construct reliable training data,the decision fusion method is used to prioritize the results of existing methods. After that,the performance of decision fusion method is validated. Furthermore,in consideration of the long range dependencies of successive expression values,Hidden Conditional Random Field model( HCRF) is selected and applied to miRNA expression profiling to infer disease-associated miRNAs. The results show that HCRF achieves superior performance and outperforms the previous methods. The results also demonstrate the power of using expression profiling for discovering disease-associated miRNAs.
基金supported by the National Science Foundation of China (81071350,81271850,and 31170155)the National Program on Key Basic Research Project (973 program 2011CB504804 and 2012CB519003)
文摘Congenital human cytomegalovirus(HCMV) infection is a leading infectious cause of birth defects.Previous studies have reported birth defects with multiple organ maldevelopment in congenital HCMV-infected neonates. Multipotent mesenchymal stromal cells(MSCs) are a group of stem/progenitor cells that are multi-potent and can self-renew, and they play a vital role in multiorgan formation. Whether MSCs are susceptible to HCMV infection is unclear. In this study, MSCs were isolated from Wharton's jelly of the human umbilical cord and identified by their plastic adherence, surface marker pattern, and differentiation capacity. Then, the MSCs were infected with the HCMV Towne strain, and infection status was assessed via determination of viral entry,replication initiation, viral protein expression, and infectious virion release using western blotting,immunofluorescence assays, and plaque forming assays. The results indicate that the isolated MSCs were fully permissive for HCMV infection and provide a preliminary basis for understanding the pathogenesis of HCMV infection in non-nervous system diseases, including multi-organ malformation during fetal development.
基金Supported by Student's Platform for Innovation and Entrepreneurship Training Program "Development of Campus Precise Navigation System Based on Mobile Terminal"(201610223065)Heilongjiang Provincial Level and Postgraduate Education Reform Research Project "Research on the Integration of Production,Study and Practice Training Mode of Agricultural Information Professional Master"(JGXM_HLJ_2014094)Heilongjiang Provincial Level and Postgraduate Education Teaching Reform Project "Training Model of Practical and Innovative Agricultural Information Professional Master"(JGXM_HLJ_2016126)
文摘This paper introduced the application of the WebGIS and virtual reality technology in the tourism industry and analyzed the existing problems in the tourism information system.Taking Daqing Oil Field Scenic Sport as an example,it introduced a method of constructing the virtual simulation platform for tourism based on the combination of WebGIS and virtual reality technology.Through the virtual simulation platform of Daqing Tourism Scenic Spot,it verified the design idea,methods,and technical feasibility of the virtual tourism system,and built a specific,feasible,and practical virtual simulation platform for tourism.It showed the bright prospects of virtual tourism development based on WebGIS and virtual reality technology.
基金jointly supported by the Key R&D Program of Jiangsu ProvinceChina(BE2022338)+3 种基金the Jiangsu Agriculture Science and Technology Innovation FundChina(CX(20)1012)the National Natural Science Foundation of China(31801293)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),China。
文摘Biaxia lrotary tillage in dryland(DBRT)can complete biaxial rotary tillage with straw incorporation,secondary suppression,and ditching,and it has been previously studied in direct-seeded rice and wheat.However,the effects of DBRT on the mechanically transplanted rice yield and greenhouse gas emissions remain unclear.To evaluate the effects of DBRT on improving the food security of mechanically transplanted rice and reducing the greenhouse gas emissions,we conducted an experiment for two years with wheat straw incorporation.Three tillage methods were set up:DBRT,uniaxial rotary tillage in dryland and paddy(DPURT),and uniaxial rotary tillage in paddy(PURT).The results showed that compared with DPURT and PURT,DBRT increased the yield of machine-transplanted rice by 7.5-11.0%and 13.3-26.7%,respectively,while the seasonal cumulative CH_(4) emissions were reduced by 13.9-21.2%and 30.2-37.0%,respectively,and the seasonal cumulative N_(2)O emissions were increased by 13.5-28.6%and 50.0-73.1%,respectively.Consequently,DBRT reduced the global warming potential by 10.7-15.5%and 23.7-28.6%,respectively,andtheyield-scaledglobalwarmingpotentialby18.2-21.8%and36.4-39.3%,respectively,compared to DPURT and PURT.These results were mainly related to the fact that DBRT significantly reduced soil bulk density and increased soil redox potential(Eh).Therefore,implementing DBRT in machine-transplanted rice fields is feasible,which cannot only increase the rice yield,but also reduce the greenhouse gas emissions.
基金supported by the Beijing Outstanding Young Scientist Project(No.C19H100010)Beijing Outstanding Talent Training Foundation,China(No.2018000020124G056)with title‘Efficient removal and toxicity study of typical antibiotics from waste water of high-speed railway trains in Beijing’the National Natural Science Foundation of China(No.52042201)。
文摘Electrochemical heterogeneous catalytic ozonation(E-catazone)is a promising and advanced oxidation technology that uses a titanium dioxide nanoflower(TiO_(2-NF))-coated porous Ti gas diffuser as an anode material.Our previous study has highlighted that the importance of the TiO_(2-NF)coating layer in enhancing*OH production and rapidly degrading O_(3)-resistant drugs.It is well known that the properties of TiO_(2-NF)are closely related to its sintering temperature.However,to date,related research has not been conducted in E-catazone systems.Thus,this study evaluated the effect of the sintering temperature on the degradation of the O_(3)-resistant drug para-chlorobenzoic acid(p-CBA)using both experimental and kinetic modeling and revealed its influence mechanism.The results indicated that the TiO_(2-NF)sintering temperature could influence p-CBA degradation and*OH production.TiO_(2-NF)prepared at 450 C showcased the highest p-CBA removal efficiency(98.5%in 5 min)at a rate of 0.82 min^(-1),and an*OH exposure of 8.41 x 10^(-10)mol L^(-1)s.Kinetic modeling results and interface characterization data revealed that the sintering temperature could alter the TiO_(2)crystallized phase and the content of surfaceadsorbed oxygen,thus affecting the two key limiting reactions in the E-catazone process.That is,≡TiO_(2)surface reacted with H_(2)O to form TiO_(2)-(OH)_(2),which then heterogeneously catalyzed O_(3)to form*OH.Consequently,E-catazone with a TiO_(2-NF)anode prepared at 450°C generated the highest surface reaction rate(5.00 x 10^(-1)s^(-1)and 4.00 x 10^(-3)L mol^(-1)s^(-1),respectively),owing to its higher anatase content and adsorbed oxygen.Thus,a rapid O_(3)-TiO_(2)reaction was achieved,resulting in an enhanced*OH formation and a highly effective p-CBA degradation.Overall,this study provides novel baseline data to improve the application of E-catazone technology.
基金the Natural Science Foundation of China(U21A20259)National Key Research and Development Program of Ningxia Hui Autonomous Region(2021BEF02028)+1 种基金the Natural Science Foundation of Hubei Province(2021CFA016)China Agriculture Research System of MOF and MARA and Basic and technical innovation team for prevention and control of bovine disease.
文摘Bovine tuberculosis(bTB)is a chronic zoonotic disease that is endemic in China.Current in-vitro tests for bTB are mainly based on blood assays.Collection of samples results in some stress to the sampled cattle and associated economic losses for the herd owner.This study was designed to investigate the relationship between milk and serum antibody tests for bTB in dairy cows using 85 cows with milk and corresponding blood samples.Totally 4,395 milk samples were used to assesse the apparent(test)prevalence and incidence of bTB using the milk antibody ELISA.The association between levels of bTB milk antibody and milk quality was also evaluated.Milk and serum antibody tests showed a good correlation with a 87.5%(95%CI:61.7%,98.4)positive agreement and 98.7%(95%CI:95.4,99.8)negative agreement.The animal level lactoprevalence ranged from 0.3%(95%CI:0,1.2)to 33.3%(95%CI:26.6,40.6)in different farms and the incidence rate ranged from 0 head/cow-month(95%CI:0,0.02)to 0.04 head/cow-month(95%CI:0.02,0.07).Twenty percent of sampled farms met the criteria for bTB control in China.The prevalence on large-scale farms was lower(p<0.001)than on small farms.The bTB milk antibody levels had a negative correlation with milk yield and a positive correlation with somatic cell count(SCC),milk protein percentage(MPP)and percentage of total solids(TS).According to this research,milk ELISA could be used as a supplement of blood samples to assist in the surveillance for bTB and for alerting control and eradication of bTB.
基金supported by the National Natural Science Foundation of China(U2166211).
文摘Decarbonisation of power systems is essential for realising carbon neutrality,in which the economic cost caused by carbon needs to be qualified.Based on the formulation of locational marginal price(LMP),this paper proposes a locational marginal electricity-carbon price(EC-LMP)model to reveal carbon-related costs caused by power consumers.A carbon-priceintegrated optimal power flow(C-OPF)is then developed to maximise economic efficiency of the power system considering the costs of electricity and carbon.Case studies are presented to demonstrate the new formulation and results demonstrate the efficacy of the EC-LMP-based C-OPF on decarbonisation and economy.
基金supported by the National Key Research and Development Program of China(2020YFB1506400)the National Natural Science Foundation of China(51922074,22075194,and 51820105003)+3 种基金the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(20KJA430010)the Tang Scholarthe Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the Collaborative Innovation Center of Suzhou Nano Science and Technology。
文摘Perovskite solar cells(pero-SCs)performance is essentially limited by severe non-radiative losses and ion migration.Although numerous strategies have been proposed,challenges remain in the basic understanding of their origins.Here,we report a dielectric-screening-enhancement effect for perovskite defects by using organic semiconductors with finely tuned molecular structures from the atoms level.Our method produced various perovskite films with high dielectric constant values,reduced charge capture regions,suppressed ion migration,and it provides an efficient charge transport pathway for suppressing non-radiative recombination beyond the passivation effect.The resulting pero-SCs showed a promising power conversion efficiency(PCE)of 23.35%with a high open-circuit voltage(1.22 V);and the 1-cm^(2) pero-SCs maintained an excellent PCE(21.93%),showing feasibility for scalable fabrication.The robust operational and thermal stabilities revealed that this method paved a new way to understand the degradation mechanism of pero-SCs,promoting the efficiency,stability and scaled fabrication of the pero-SCs.
基金supported by the National Natural Science Foundation of China (31471548,32272179,and 31801404)the Central Plains Science and Technology Innovation Leader Project (214200510029)+4 种基金the Program for Innovative Research Team (in Science and Technology)in University of Henan Province (20IRTSTHN021)the Science and Technology Development Project of Anyang City (2022C01NY001 and 2022C01NY003)the Doctoral and Postdoctoral Research Fund of Anyang Institute of Technology (BSJ2019014 and BHJ2020002)the Key Scientific Research Project of Henan Higher Education Institutions of China (20A210006)the Zhongyuan Scholars Workstation (224400510020).
文摘Increasing soil salinization has led to severe reductions in plant yield and quality,and investigating the mo-lecular mechanism of salt stress response is therefore an urgent priority.In this study,we systematically analyzed the response of cotton roots to salt stress using single-cell transcriptomics technology;56281 high-quality cells were obtained from 5-day-old lateral root tips of Gossypium arboreum under natural growth conditions and different salt treatments.Ten cell types with an array of novel marker genes were identified and confirmed by in situ RNA hybridization,and pseudotime analysis of some specific cell types revealed their potential differentiation trajectories.Prominent changes in cell numbers under salt stress were observed for outer epidermal and inner endodermal cells,which were significantly enriched in response to stress,amide biosynthetic process,glutathione metabolism,and glycolysis/gluconeogenesis.Analysis of differentially expressed genes identified in multiple comparisons revealed other functional ag-gregations concentrated on plant-type primary cell wall biogenesis,defense response,phenylpropanoid biosynthesis,and metabolic pathways.Some candidate differentially expressed genes encoding transcrip-tion factors or associated with plant hormones also responsive to salt stress were identified,and the func-tion of Ga03G2153,annotated as auxin-responsive GH3.6,was confirmed by virus-induced gene silencing.The GaGH3.6-silenced plants showed a severe stress-susceptible phenotype,and physiological and biochemical measurements indicated that they suffered more significant oxidative damage.These results suggest that GaGH3.6 might participate in cotton salt tolerance by regulating redox processes.We thus construct a transcriptional atlas of salt-stressed cotton roots at single-cell resolution,enabling us to explore cellular heterogeneity and differentiation trajectories and providing valuable insights into the mo-lecular mechanisms that underlie plant stress tolerance.
基金support from the National Natural Science Foundation of China(Nos.22075313 and 21975281)the National Key Research and Development Program of China(No.2020YFB1312902)+3 种基金the Science and Technology Project of Jiangxi Province(No.20192BCD40017)Outstanding Youth Fund of Jiangxi Province(No.20192BCB23028)Jiangxi Double Thousand Talent Program(No.JXSQ2019101072)Science Technology Major Project of Nanchang(No.2020BI47)is acknowledged.
文摘Aqueous zinc battery has been regarded as one of the most promising energy storage systems due to its low cost and environmental benignity.However,the safety concern on Zn anodes caused by uncontrolled Zn dendrite growth in aqueous electrolyte hinders their application.Herein,sucrose with multi-hydroxyl groups has been introduced into aqueous electrolyte to modify Zn^(2+)solvation environment and create a protection layer on Zn anode,thus effectively retarding the growth of zinc dendrites.Atomistic simulations and experiments confirm that sucrose molecules can enter into the solvation sheath of Zn^(2+),and the as-formed unique solvation structure enhances the mobility of Zn^(2+).Such fast Zn^(2+)kinetics in sucrose-modified electrolyte can successfully suppress the dendrite growth.With this sucrose-modified aqueous electrolyte,Zn/Zn symmetric cells present more stable cycle performance than those using pure aqueous electrolyte;Zn/C cells also deliver an impressive higher energy density of 129.7 Wh·kg^(−1)and improved stability,suggesting a great potential application of sucrose-modified electrolytes for future Zn batteries.
基金This work was supported by the National Natural Science Foundation of China(Grant No.51273122)the Sichuan Science and Technology Project(Grant No.2018JY0535).
文摘An aligned composite film was fabricated via the deposition of carboxylic graphe ne oxide(C-GO)and polypyrrole(PPy)nano particles on alig ned poly(L-lactic acid)(PLLA)fiber-films(named as C-GO/PPy/PLLA),which has the core(PLLA)-sheath(C-GO/PPy)structure,and the composition of C-GO(~4.8 wt.%of PPy sheath)significantly enhanced the tensile strength and the conductivity of the PPy/PLLA film?Especially,after 4 weeks of immersion in the PBS solution,the conductivity and the tensile strength of CGO/PPy/PLLA films still remained-6.10 S/cm and 28.9 MPa,respectively,which could meet the need of the sustained electrical stimulation(ES)therapy for nerve repair.Moreover,the neurite length and the neurite alignment were significantly in creased through exerting ES on C-GO/PPy/PLLA films due to their sustained conductivity in the fluid of cell culture.These results indicated that C-GO/PPy/PLLA with sustained conductivity and mechanical property possessed great potential of nerve repair by exerting lasting-ES.
基金This work was supported by the Natural Science Foundation of Shandong Province(ZR2015BM024)“Shandong Province Tai-Shan Program”,China Scholarship Council(CSC)scholarship to Dr.Hong,and the National Natural Science Foundation of China(21729501).
文摘A sticky-flare gold nanoparticle probe(AuNP-probe)is designed by the combination of locked nucleic acid functionalized silencing of microRNA technology for intracellular microRNA-21(miRNA-21)sensitively detecting,fluorescence imaging,localizing and silencing.The limit of detection is as low as 0.01 n M.Overexpressed miRNA-21 in cancer cells serves as endogenous drug release stimuli to trigger the release of probe-loaded doxorubicin(Dox),which soon translocates into cell nuclei.This multifunctional Dox-loaded AuNP-probe(Dox-AuNP-probe)could induce cancer cell apoptosis effectively through the synergistic effect of gene silencing and chemotherapy.This Dox-AuNP-probe exhibits superior drug potency compared to free Dox molecules,with a cell inhibition rate of 57%(but only 20%for Dox)to wild-type cancer cells and 30%(but 0%for Dox)to drug-resistent cancer cells after 72 h,and this strategy not only has the function of sensing,but also can effectively bypass drug resistance.In MCF-7 xenograft tumor-bearing mice,the Dox-AuNP-probes show greater inhibition for tumor tissues than miRNA-21 targeted AuNP-probes(Targeting-AuNP-probe)or free Dox molecules.Therefore,the Dox-AuNP-probe represents a promising nanotheranostic platform for future applications in cancer molecular imaging and therapy,especially providing a potential strategy to treat resistant cancers.
文摘In the original publication the email addresses of corresponding authors have not been displayed.The correct email addresses of corresponding authors are provided in this correction.Fang-Cheng Li(sjwklfc@126.com),Fei Hu(neuron111@163.com),Min-Hua Luo(luomh@wh.iov.cn).
文摘Dear Editor,Gliomas are the most common brain tumors in adults which encompass all primary central nervous system(CNS)tumors of glial cell origin.The World Health Organization(WHO)classifies gliomas into four grades based on the histologic/prognostic features.Because of the unclear etiol-ogy and pathogenesis,therapeutic efficacy and prognosis is poor.
