全面跟踪回顾、梳理和综述协同推进降碳、减污、扩绿和增长相关研究对在保证经济可持续发展同时实现污染物和温室气体长效减排、自然环境持续恢复具有重要支撑作用.针对现有综述研究概念机制考虑不全以及多样性计量、评估和路径规划视...全面跟踪回顾、梳理和综述协同推进降碳、减污、扩绿和增长相关研究对在保证经济可持续发展同时实现污染物和温室气体长效减排、自然环境持续恢复具有重要支撑作用.针对现有综述研究概念机制考虑不全以及多样性计量、评估和路径规划视角缺乏整合总结的不足,采用Citespace软件对2000~2024年中国知网和Web of Science数据库相关文献进行梳理分析,捕捉降碳、减污、扩绿和增长多要素协同相关研究的关键知识基础和主题演变规律,并对相关领域前沿应用研究进行详细回顾和系统整合剖析.结果表明:(1)降碳、减污、扩绿和增长多要素协同相关研究在21世纪以来呈现显著上升趋势,中国和美国是发文量最多的两个国家;(2)热门研究领域主要集中在多要素的协同机制、综合评估和协同发展路径这3个方面,研究框架可分为基于多维指标体系的综合评价、生态系统服务价值实现框架下的多要素协调研究和复杂多要素巨系统的模拟优化;(3)未来研究重点将聚焦协同推进降碳、减污、扩绿和增长的概念内涵整合界定、从区域和政策两个层面的多要素多尺度的协同评估以及多要素协同如何支撑“双碳”和美丽中国目标达成等方向.展开更多
OBJECTIVE:To investigate the therapeutic effects of Huai'er(Trametes)on psoriasis by identifying specific molecular targets and pathways involved in regulating keratinocyte behavior and immune responses.METHODS:Ce...OBJECTIVE:To investigate the therapeutic effects of Huai'er(Trametes)on psoriasis by identifying specific molecular targets and pathways involved in regulating keratinocyte behavior and immune responses.METHODS:Cellular experiments were conducted using human keratinocyte cell line(Ha Ca T)keratinocytes to evaluate the effects of Huai'er(Trametes)on cell proliferation,migration,and inflammatory factor expression.Additionally,an imiquimod(IMQ)-induced psoriasis-like mouse model was established to assess the therapeutic efficacy of Huai'er(Trametes)in vivo.Network pharmacology and transcriptomic analyses were performed to identify potential targets and pathways.RESULTS:Huai'er(Trametes)significantly inhibited Ha Ca T keratinocyte proliferation and migration in vitro,as evidenced by reduced 5-ethynyl-2′-deoxyuridine incorporation and wound healing rates.In the IMQ-induced psoriasis-like mouse model,Huai'er(Trametes)treatment reduced erythema,scaling,and dermal infiltration of inflammatory cells,demonstrating its efficacy in alleviating psoriasis symptoms.Network pharmacology and transcriptomic analyses identified signal transducer and activator of transcription 1(STAT1)as a central target modulated by Huai'er(Trametes).This regulation was associated with decreased expression of proinflammatory cytokines,including interleukin-17A and tumor necrosis factor alpha,both in vitro and in vivo.CONCLUSION:This study demonstrates that Huai'er(Trametes)exerts therapeutic effects on psoriasis by modulating STAT1,thereby inhibiting keratinocyte proliferation and inflammatory responses.The findings provide a foundation for further research into the potential of Huai'er(Trametes)as a treatment for psoriasis.展开更多
Genistein has a neuroprotective effect in Alzheimer＇s disease, but its mechanism of action needs further clarification. Accumulating evidence suggests that excessive phosphorylation of tau protein causes production o...Genistein has a neuroprotective effect in Alzheimer＇s disease, but its mechanism of action needs further clarification. Accumulating evidence suggests that excessive phosphorylation of tau protein causes production of neurofibrillary tangles, which is one of the main pathological characteristics of Alzheimer＇s disease, and tau protein can be phosphorylated by calcium/calmodulin dependent protein kinase IV （CAMK4）. After 7 days of pre-administration of genistein （90 mg/kg）, an Alzheimer＇s disease rat model was established using an intraperitoneal injection of D-galactose combined with an intracerebral injection of amyloid-β peptide （25-35）. The rat was then continu- ously administered genistein （90 mg/kg） for 42 days. The Morris water maze test, western blotting and hematoxylin-eosin staining results showed that genistein significantly decreased the escape latency and increased the number of times crossing the platform, reduced p-tau, CALM, CAMKK1 and p-CAMK4 protein levels in the hippocampus, and alleviated hippocampal neuron damage. These findings indicate that genistein may play a neuroprotective role in Alzheimer＇s disease through regulating CAMK4 to modulate tau hyperphosphorylation. Key展开更多
文摘全面跟踪回顾、梳理和综述协同推进降碳、减污、扩绿和增长相关研究对在保证经济可持续发展同时实现污染物和温室气体长效减排、自然环境持续恢复具有重要支撑作用.针对现有综述研究概念机制考虑不全以及多样性计量、评估和路径规划视角缺乏整合总结的不足,采用Citespace软件对2000~2024年中国知网和Web of Science数据库相关文献进行梳理分析,捕捉降碳、减污、扩绿和增长多要素协同相关研究的关键知识基础和主题演变规律,并对相关领域前沿应用研究进行详细回顾和系统整合剖析.结果表明:(1)降碳、减污、扩绿和增长多要素协同相关研究在21世纪以来呈现显著上升趋势,中国和美国是发文量最多的两个国家;(2)热门研究领域主要集中在多要素的协同机制、综合评估和协同发展路径这3个方面,研究框架可分为基于多维指标体系的综合评价、生态系统服务价值实现框架下的多要素协调研究和复杂多要素巨系统的模拟优化;(3)未来研究重点将聚焦协同推进降碳、减污、扩绿和增长的概念内涵整合界定、从区域和政策两个层面的多要素多尺度的协同评估以及多要素协同如何支撑“双碳”和美丽中国目标达成等方向.
基金the Scientific Research Project of Hunan Provincial Health Commission:Effects and Mechanisms of Huaiqi Huang on the Proliferation and Migration of Psoriasis HaCaT Cells(No.D202304128233)。
文摘OBJECTIVE:To investigate the therapeutic effects of Huai'er(Trametes)on psoriasis by identifying specific molecular targets and pathways involved in regulating keratinocyte behavior and immune responses.METHODS:Cellular experiments were conducted using human keratinocyte cell line(Ha Ca T)keratinocytes to evaluate the effects of Huai'er(Trametes)on cell proliferation,migration,and inflammatory factor expression.Additionally,an imiquimod(IMQ)-induced psoriasis-like mouse model was established to assess the therapeutic efficacy of Huai'er(Trametes)in vivo.Network pharmacology and transcriptomic analyses were performed to identify potential targets and pathways.RESULTS:Huai'er(Trametes)significantly inhibited Ha Ca T keratinocyte proliferation and migration in vitro,as evidenced by reduced 5-ethynyl-2′-deoxyuridine incorporation and wound healing rates.In the IMQ-induced psoriasis-like mouse model,Huai'er(Trametes)treatment reduced erythema,scaling,and dermal infiltration of inflammatory cells,demonstrating its efficacy in alleviating psoriasis symptoms.Network pharmacology and transcriptomic analyses identified signal transducer and activator of transcription 1(STAT1)as a central target modulated by Huai'er(Trametes).This regulation was associated with decreased expression of proinflammatory cytokines,including interleukin-17A and tumor necrosis factor alpha,both in vitro and in vivo.CONCLUSION:This study demonstrates that Huai'er(Trametes)exerts therapeutic effects on psoriasis by modulating STAT1,thereby inhibiting keratinocyte proliferation and inflammatory responses.The findings provide a foundation for further research into the potential of Huai'er(Trametes)as a treatment for psoriasis.
基金supported by the National Natural Science Foundation of China,No.81202941 and 81574040the Key Project Foundation of Oversea Visiting and Research for the Excellent Young and Middle-aged Faculties in Universities of Anhui Province in China,No.gxfx ZD2016119+1 种基金the Key Project Foundation of Natural Science Research in Universities of Anhui Province in China,No.KJ2016A406the Key Project Foundation of Support Program for the Excellent Young Faculties in Universities of Anhui Province in China,No.gxyq ZD2016138
文摘Genistein has a neuroprotective effect in Alzheimer＇s disease, but its mechanism of action needs further clarification. Accumulating evidence suggests that excessive phosphorylation of tau protein causes production of neurofibrillary tangles, which is one of the main pathological characteristics of Alzheimer＇s disease, and tau protein can be phosphorylated by calcium/calmodulin dependent protein kinase IV （CAMK4）. After 7 days of pre-administration of genistein （90 mg/kg）, an Alzheimer＇s disease rat model was established using an intraperitoneal injection of D-galactose combined with an intracerebral injection of amyloid-β peptide （25-35）. The rat was then continu- ously administered genistein （90 mg/kg） for 42 days. The Morris water maze test, western blotting and hematoxylin-eosin staining results showed that genistein significantly decreased the escape latency and increased the number of times crossing the platform, reduced p-tau, CALM, CAMKK1 and p-CAMK4 protein levels in the hippocampus, and alleviated hippocampal neuron damage. These findings indicate that genistein may play a neuroprotective role in Alzheimer＇s disease through regulating CAMK4 to modulate tau hyperphosphorylation. Key
