Banana fruit ripening is a highly regulatory process involving various layers consisting of transcriptional regulation,epigenetic factor,and post-translational modification.Previously,we reported that MaERF11 cooperat...Banana fruit ripening is a highly regulatory process involving various layers consisting of transcriptional regulation,epigenetic factor,and post-translational modification.Previously,we reported that MaERF11 cooperated with MaHDA1 to precisely regulate the transcription of ripening-associated genes via histone deacetylation.However,whether MaERF11 is subjected to post-translational modification during banana ripening is largely unknown.In this study,we found that MaERF11 targeted a subset of starch degradation-related genes using the DNA affinity purification sequence(DAP-Seq)approach.Electrophoretic mobility shift assay(EMSA)and dual-luciferase reporter assay(DLR)demonstrated that MaERF11 could specifically bind and repress the expression of the starch degradation-related genes MaAMY3,MaBAM2 and MaGWD1.Further analyses of yeast two-hybrid(Y2H),bimolecular fluorescence complementation(BiFC)and Luciferase complementation imaging(LCI)assays indicated that MaERF11 interacted with the ubiquitin E3 ligase MaRFA1,and this interaction weakened the MaERF11-mediated transcriptional repression capacity.Collectively,our results suggest an additional regulatory layer in which MaERF11 regulates banana fruit ripening and expands the regulatory network in fruit ripening at the post-translational modification level.展开更多
Guangdong Province is one of the provinces in China with the best modern agricultural industry and the highest production efficiency.While Jiangmen City,one of the agriculture-based cities in Guangdong Province,has ma...Guangdong Province is one of the provinces in China with the best modern agricultural industry and the highest production efficiency.While Jiangmen City,one of the agriculture-based cities in Guangdong Province,has made active exploration in building a modern agricultural system.China has established cooperation with the four largest agricultural research institutes in China to offer technical support for the development of modern agriculture.In this paper,the status quo and existing problems of agricultural development in Jiangmen,Guangdong are briefly introduced.Then,the cooperation status and prospect of local government with the research institutes are discussed.Finally some suggestions are given on the agricultural development pattern in Jiangmen City.展开更多
In recent years,Millettia specisoa Champ has attracted extensive attention because of its high medicinal and edible value. This paper will summarize the composition and application of Millettia specisoa Champ,analyze ...In recent years,Millettia specisoa Champ has attracted extensive attention because of its high medicinal and edible value. This paper will summarize the composition and application of Millettia specisoa Champ,analyze the advantages and existing problems in developing Millettia specisoa Champ industry in Guangdong Province according to the demand for Millettia specisoa Champ and the present situation in Guangdong Province,and put forward reasonable countermeasures and suggestions.展开更多
This paper introduces the origin and progress of Tropical and South Subtropical Agricultural Comprehensive Experimental Station( hereinafter referred to as experimental station) CATAS( Jiangmen),formulates the thought...This paper introduces the origin and progress of Tropical and South Subtropical Agricultural Comprehensive Experimental Station( hereinafter referred to as experimental station) CATAS( Jiangmen),formulates the thought for construction of experimental station,analyzes the restrictive factors and puts forward corresponding countermeasures in order to provide reference for the decision- making and implementation of constructing the experimental station.展开更多
Bananas(Musa ssp.)are globally important staple crops increasingly constrained by biotic stressors,climatic instability,and the high labor demands of cultivation.The genetic improvement of dwarf phenotypes offers a st...Bananas(Musa ssp.)are globally important staple crops increasingly constrained by biotic stressors,climatic instability,and the high labor demands of cultivation.The genetic improvement of dwarf phenotypes offers a strategic pathway to enhance mechanization and reduce production costs.In this study,we have carried out whole-genome resequencing of 300 Musa accessions to analyze genome-wide allelic diversity and identify loci associated with shoot architecture.Our analysis uncovered extensive genetic variation within the A subgenome,pivotal for environmental adaptability,and detected introgression from Musa itinerans(subgroup A)into cultivated varieties(subgroup F),suggesting a broadened genetic base amenable to breeding.A genome-wide association study(GWAS)pinpointed MabHLH30 as a crucial gene associated plant stature.Functional validation confirmed MabHLH30 as a critical regulator of plant stature and leaf morphology.Leveraging this finding,we developed molecular markers for MabHLH30,enabling marker-assisted selection(MAS)to accelerate the breeding of compact,high-yielding cultivars.Collectively,these results provide a genomic framework for the targeted improvement of banana architecture and represent a valuable resource for cultivar development under diverse agroecological conditions.展开更多
基金financially supported by the National Natural Science Foundation of China(Grant Nos.31830071,32202561)the earmarked fund for CARS(Grant No.CARS-31)。
文摘Banana fruit ripening is a highly regulatory process involving various layers consisting of transcriptional regulation,epigenetic factor,and post-translational modification.Previously,we reported that MaERF11 cooperated with MaHDA1 to precisely regulate the transcription of ripening-associated genes via histone deacetylation.However,whether MaERF11 is subjected to post-translational modification during banana ripening is largely unknown.In this study,we found that MaERF11 targeted a subset of starch degradation-related genes using the DNA affinity purification sequence(DAP-Seq)approach.Electrophoretic mobility shift assay(EMSA)and dual-luciferase reporter assay(DLR)demonstrated that MaERF11 could specifically bind and repress the expression of the starch degradation-related genes MaAMY3,MaBAM2 and MaGWD1.Further analyses of yeast two-hybrid(Y2H),bimolecular fluorescence complementation(BiFC)and Luciferase complementation imaging(LCI)assays indicated that MaERF11 interacted with the ubiquitin E3 ligase MaRFA1,and this interaction weakened the MaERF11-mediated transcriptional repression capacity.Collectively,our results suggest an additional regulatory layer in which MaERF11 regulates banana fruit ripening and expands the regulatory network in fruit ripening at the post-translational modification level.
基金Supported by the Special Fund for the Construction of Modern Agricultural Technical System(CARS-12-gdqxd)the Special Foundation for the Basic Scientific Research of Central Public Welfare Institute(1630032013033)
文摘Guangdong Province is one of the provinces in China with the best modern agricultural industry and the highest production efficiency.While Jiangmen City,one of the agriculture-based cities in Guangdong Province,has made active exploration in building a modern agricultural system.China has established cooperation with the four largest agricultural research institutes in China to offer technical support for the development of modern agriculture.In this paper,the status quo and existing problems of agricultural development in Jiangmen,Guangdong are briefly introduced.Then,the cooperation status and prospect of local government with the research institutes are discussed.Finally some suggestions are given on the agricultural development pattern in Jiangmen City.
基金Supported by Special Project of Fundamental Research Funds for Central Public Welfare Research Institutes in Chinese Academy of Tropical Agricultural Sciences(1630032012047)Special Project of Fundamental Research Funds for Central Public Welfare Research Institutes in Chinese Academy of Tropical Agricultural Sciences(1630032014006)
文摘In recent years,Millettia specisoa Champ has attracted extensive attention because of its high medicinal and edible value. This paper will summarize the composition and application of Millettia specisoa Champ,analyze the advantages and existing problems in developing Millettia specisoa Champ industry in Guangdong Province according to the demand for Millettia specisoa Champ and the present situation in Guangdong Province,and put forward reasonable countermeasures and suggestions.
文摘This paper introduces the origin and progress of Tropical and South Subtropical Agricultural Comprehensive Experimental Station( hereinafter referred to as experimental station) CATAS( Jiangmen),formulates the thought for construction of experimental station,analyzes the restrictive factors and puts forward corresponding countermeasures in order to provide reference for the decision- making and implementation of constructing the experimental station.
基金supported by the Research Program of Sanya Yazhou Bay Science and Technology City(SYND-2021-05,SYND-2022-13)the Natural Science Foundation of Hainan(321MS066)+4 种基金the Central Public-interest Scientific Institution Basal Research Fund(1630052022006,CATASCXTD202309)the National Natural Science Foundation of China(32360706,U22A20487)the Project of National Key Laboratory for Tropical Crop Breeding(NKLTCBCXTD27,NKLTCB202311)the Social Public-Interest Scientific Institution Reform Special Fund(1630052024003)the China Agriculture Research System(CARS-31)。
文摘Bananas(Musa ssp.)are globally important staple crops increasingly constrained by biotic stressors,climatic instability,and the high labor demands of cultivation.The genetic improvement of dwarf phenotypes offers a strategic pathway to enhance mechanization and reduce production costs.In this study,we have carried out whole-genome resequencing of 300 Musa accessions to analyze genome-wide allelic diversity and identify loci associated with shoot architecture.Our analysis uncovered extensive genetic variation within the A subgenome,pivotal for environmental adaptability,and detected introgression from Musa itinerans(subgroup A)into cultivated varieties(subgroup F),suggesting a broadened genetic base amenable to breeding.A genome-wide association study(GWAS)pinpointed MabHLH30 as a crucial gene associated plant stature.Functional validation confirmed MabHLH30 as a critical regulator of plant stature and leaf morphology.Leveraging this finding,we developed molecular markers for MabHLH30,enabling marker-assisted selection(MAS)to accelerate the breeding of compact,high-yielding cultivars.Collectively,these results provide a genomic framework for the targeted improvement of banana architecture and represent a valuable resource for cultivar development under diverse agroecological conditions.
