Objective. Syndecan- 1 binds to various extracellular matrix components via its heparan sulfate glycosaminoglycans (HS- GAG) and most of its biological functions are considered to be associated with this process. The ...Objective. Syndecan- 1 binds to various extracellular matrix components via its heparan sulfate glycosaminoglycans (HS- GAG) and most of its biological functions are considered to be associated with this process. The aims of this study are to investigate its expression in cervical neoplasms. Methods. We investigated the expression of both the syndecan- 1 core protein and cell- surface HS- GAG by immunohistochemistry in 53 cervical intraepithelial neoplasm (CIN), 19 microinvasive, 143 invasive cervical cancers, and 29 metastatic lymph node samples, and analyzed correlations with various clinicopathological features. Results. The progression of CIN to early invasive cancer was found to associate with reduced levels of both syndecan- 1 and HS- GAG expression. In squamous cell carcinomas, HS- GAG expression was significantly lower in cases with lymph- vascular space invasion. Additionally, the overall survival rates for patients exhibiting low HS- GAG expression was significantly lower than patients exhibiting high HS- GAG expression (P = 0.019). Low HS- GAG expression in positive nodes was determined to be a disease- free and overall survival prognostic factor (P = 0.028 and P = 0.018, respectively). Conclusion. The loss of syndecan- 1 and HS- GAG expression is an early event in cervical carcinogenesis. The loss of HS- GAG expression particularly in positive nodes can serve as an indicator of aggressive disease potential and poor prognosis in patients with invasive cervical cancer.展开更多
Objective: In this study, we measured both thymidine phosphorylase (TP) and dihydropyrimidine dehydrogenase (DPD) levels, and then determined the TP/DPD ratios, in a number of invasive cervical cancers. Our aim was to...Objective: In this study, we measured both thymidine phosphorylase (TP) and dihydropyrimidine dehydrogenase (DPD) levels, and then determined the TP/DPD ratios, in a number of invasive cervical cancers. Our aim was to investigate the association between these values and the clinicopathological features of invasive cervical cancer, including patient prognosis. Study design: Using enzyme-linked immunosorbent assays, the TP and DPD activities were quantitatively measured in 51 patients with cervical cancer who had also undergone a radical hysterectomy. Results: The TP, DPD and TP/DPD values in squamous cell carcinoma (SCC) were found to be significantly higher than the levels in non-SCC. Furthermore, in a SCC subgroup, the TP/DPD levels were significantly higher in cases where parametrial invasion was evident, and in a non-SCC subgroup, these levels were also significantly higher in cases with deep stromal invasion, lymph-vascular space invasion, large tumor size and parametrial invasion. The disease-free survival rate is shown to be significantly higher in patients with low TP/DPD levels indicating that this ratio may be associated with both the progression and recurrence of invasive cervical cancer. Conclusion: TP/DPD ratios may be associated with both the progression and recurrence of invasive cervical cancer.展开更多
文摘Objective. Syndecan- 1 binds to various extracellular matrix components via its heparan sulfate glycosaminoglycans (HS- GAG) and most of its biological functions are considered to be associated with this process. The aims of this study are to investigate its expression in cervical neoplasms. Methods. We investigated the expression of both the syndecan- 1 core protein and cell- surface HS- GAG by immunohistochemistry in 53 cervical intraepithelial neoplasm (CIN), 19 microinvasive, 143 invasive cervical cancers, and 29 metastatic lymph node samples, and analyzed correlations with various clinicopathological features. Results. The progression of CIN to early invasive cancer was found to associate with reduced levels of both syndecan- 1 and HS- GAG expression. In squamous cell carcinomas, HS- GAG expression was significantly lower in cases with lymph- vascular space invasion. Additionally, the overall survival rates for patients exhibiting low HS- GAG expression was significantly lower than patients exhibiting high HS- GAG expression (P = 0.019). Low HS- GAG expression in positive nodes was determined to be a disease- free and overall survival prognostic factor (P = 0.028 and P = 0.018, respectively). Conclusion. The loss of syndecan- 1 and HS- GAG expression is an early event in cervical carcinogenesis. The loss of HS- GAG expression particularly in positive nodes can serve as an indicator of aggressive disease potential and poor prognosis in patients with invasive cervical cancer.
文摘Objective: In this study, we measured both thymidine phosphorylase (TP) and dihydropyrimidine dehydrogenase (DPD) levels, and then determined the TP/DPD ratios, in a number of invasive cervical cancers. Our aim was to investigate the association between these values and the clinicopathological features of invasive cervical cancer, including patient prognosis. Study design: Using enzyme-linked immunosorbent assays, the TP and DPD activities were quantitatively measured in 51 patients with cervical cancer who had also undergone a radical hysterectomy. Results: The TP, DPD and TP/DPD values in squamous cell carcinoma (SCC) were found to be significantly higher than the levels in non-SCC. Furthermore, in a SCC subgroup, the TP/DPD levels were significantly higher in cases where parametrial invasion was evident, and in a non-SCC subgroup, these levels were also significantly higher in cases with deep stromal invasion, lymph-vascular space invasion, large tumor size and parametrial invasion. The disease-free survival rate is shown to be significantly higher in patients with low TP/DPD levels indicating that this ratio may be associated with both the progression and recurrence of invasive cervical cancer. Conclusion: TP/DPD ratios may be associated with both the progression and recurrence of invasive cervical cancer.
