[Objectives]To establish a high-performance liquid chromatography(HPLC)fingerprint analysis method for Hedyotis hedyotidea,providing a scientific basis for its quality evaluation.[Methods]An Agilent ZORBAX SB-C_(18)co...[Objectives]To establish a high-performance liquid chromatography(HPLC)fingerprint analysis method for Hedyotis hedyotidea,providing a scientific basis for its quality evaluation.[Methods]An Agilent ZORBAX SB-C_(18)column(5μm,4.6 mm×250 mm)was employed with 0.1%phosphoric acid-acetonitrile solution as the mobile phase for gradient elution.The column temperature was maintained at 40℃,detection wavelength at 240 nm,flow rate at 0.8 mL/min,and injection volume at 10μL.The Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(2004A edition)was used to analyze the HPLC fingerprints of 10 batches of H.hedyotidea samples.[Results]An HPLC fingerprint with ursolic acid as the reference standard was established,identifying 17 common characteristic peaks.The similarity of the 10 batches of H.hedyotidea samples ranged from 0.718 to 0.993.[Conclusions]This method demonstrates good systematic performance,with precision(RSD<1.5%),repeatability(RSD<2.0%),and 24-h stability(RSD<2.5%)meeting established requirements.It can provide data support for quality control and standard formulation of H.hedyotidea medicinal materials.展开更多
[Objectives]To study the microscopic characteristics and routine detection of Hedyotis hedyotidea(DC.)Merr.[Methods]The microscopic characteristics of the root,stem cross section and the whole plant powder of H.hedyot...[Objectives]To study the microscopic characteristics and routine detection of Hedyotis hedyotidea(DC.)Merr.[Methods]The microscopic characteristics of the root,stem cross section and the whole plant powder of H.hedyotidea were studied by the methods of slide-shaft section,biological staining and microscopic imaging;the routine detection items such as moisture,ash,acid-insoluble ash and extract were detected and analyzed in accordance with the requirements of Chinese Pharmacopoeia.[Results]The microscopic characteristics of the root,the stem and the whole plant powder of H.hedyotidea were obtained.The moisture content of 10 batches of H.hedyotidea samples was in the range of 4.25%-7.90%,the water-soluble extract is in the range of 15.08%-22.52%,the total ash was in the scope of 8.27%-10.45%,and the acid-insoluble ash was in 0.13%-0.95%.The proposed water content of H.hedyotidea should not exceed 10.00%,the water-soluble extract should not be less than 12.00%,the total ash content should not exceed 13.00%,and the acid-insoluble ash content should not exceed 1.20%.[Conclusions]The results of this experiment can provide a reference for the quality control and quality standard of H.hedyotidea.展开更多
[Objectives]To explore the effect of storage time on pH and 5-hydroxymethylfurfural content in Jiulongteng honey.[Methods]The pH of Jiulongteng honey was determined by neutralization titration with sodium hydroxide st...[Objectives]To explore the effect of storage time on pH and 5-hydroxymethylfurfural content in Jiulongteng honey.[Methods]The pH of Jiulongteng honey was determined by neutralization titration with sodium hydroxide standard solution.The content of 5-hydroxymethylfurfural in Jiulongteng honey was determined by HPLC.Chromatographic conditions:ZORBAX SB-C 18 column(250 mm×4.6 mm,5μm)from Agilent Co.,Ltd.,acetonitrile-0.1%formic acid solution(5:95)as mobile phase,flow rate of 0.8 mL/min,5-hydroxymethylfurfural detection wavelength of 284 nm,guanosine detection wavelength of 254 nm.[Results]The pH of 12 batches of Jiulongteng honey was 3.70-3.84 in the new honey stage,3.92-4.05 in the old honey stage 1,and 4.25-4.53 in the old honey stage 2;5-hydroxymethylfurfural was not detected in the new honey stage,5-hydroxymethylfurfural was detected in FM-001 in the old honey stage 1,and 5-hydroxymethylfurfural was detected in most samples in the old honey stage 2.[Conclusions]The pH and 5-hydroxymethylfurfural content of 12 batches of Jiulongteng honey met the requirements within 3 years of storage.There was no 5-hydroxymethylfurfural in Jiulongteng honey,but with the extension of storage time,the detection amount of 5-hydroxymethylfurfural increased significantly even if Jiulongteng honey was stored at low temperature.Therefore,5-hydroxymethylfurfural can be used as an important indicator of honey freshness.展开更多
基金Supported by Guangxi University of Chinese Medicine 2019-2021 Guangxi First-class Discipline Construction Open Project(2019XK096).
文摘[Objectives]To establish a high-performance liquid chromatography(HPLC)fingerprint analysis method for Hedyotis hedyotidea,providing a scientific basis for its quality evaluation.[Methods]An Agilent ZORBAX SB-C_(18)column(5μm,4.6 mm×250 mm)was employed with 0.1%phosphoric acid-acetonitrile solution as the mobile phase for gradient elution.The column temperature was maintained at 40℃,detection wavelength at 240 nm,flow rate at 0.8 mL/min,and injection volume at 10μL.The Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(2004A edition)was used to analyze the HPLC fingerprints of 10 batches of H.hedyotidea samples.[Results]An HPLC fingerprint with ursolic acid as the reference standard was established,identifying 17 common characteristic peaks.The similarity of the 10 batches of H.hedyotidea samples ranged from 0.718 to 0.993.[Conclusions]This method demonstrates good systematic performance,with precision(RSD<1.5%),repeatability(RSD<2.0%),and 24-h stability(RSD<2.5%)meeting established requirements.It can provide data support for quality control and standard formulation of H.hedyotidea medicinal materials.
基金Supported by 2019-2021 Guangxi First-class Discipline Construction Open Project of Guangxi University of Chinese Medicine(2019XK096).
文摘[Objectives]To study the microscopic characteristics and routine detection of Hedyotis hedyotidea(DC.)Merr.[Methods]The microscopic characteristics of the root,stem cross section and the whole plant powder of H.hedyotidea were studied by the methods of slide-shaft section,biological staining and microscopic imaging;the routine detection items such as moisture,ash,acid-insoluble ash and extract were detected and analyzed in accordance with the requirements of Chinese Pharmacopoeia.[Results]The microscopic characteristics of the root,the stem and the whole plant powder of H.hedyotidea were obtained.The moisture content of 10 batches of H.hedyotidea samples was in the range of 4.25%-7.90%,the water-soluble extract is in the range of 15.08%-22.52%,the total ash was in the scope of 8.27%-10.45%,and the acid-insoluble ash was in 0.13%-0.95%.The proposed water content of H.hedyotidea should not exceed 10.00%,the water-soluble extract should not be less than 12.00%,the total ash content should not exceed 13.00%,and the acid-insoluble ash content should not exceed 1.20%.[Conclusions]The results of this experiment can provide a reference for the quality control and quality standard of H.hedyotidea.
基金Supported by Young and Middle-aged Teachers Scientific Research Basic Ability Improvement Project in Universities of Guangxi in 2020 (2020 KY07040)School-level Scientific Research Project of Guangxi University of Chinese Medicine in 2021 (2021MS010).
文摘[Objectives]To explore the effect of storage time on pH and 5-hydroxymethylfurfural content in Jiulongteng honey.[Methods]The pH of Jiulongteng honey was determined by neutralization titration with sodium hydroxide standard solution.The content of 5-hydroxymethylfurfural in Jiulongteng honey was determined by HPLC.Chromatographic conditions:ZORBAX SB-C 18 column(250 mm×4.6 mm,5μm)from Agilent Co.,Ltd.,acetonitrile-0.1%formic acid solution(5:95)as mobile phase,flow rate of 0.8 mL/min,5-hydroxymethylfurfural detection wavelength of 284 nm,guanosine detection wavelength of 254 nm.[Results]The pH of 12 batches of Jiulongteng honey was 3.70-3.84 in the new honey stage,3.92-4.05 in the old honey stage 1,and 4.25-4.53 in the old honey stage 2;5-hydroxymethylfurfural was not detected in the new honey stage,5-hydroxymethylfurfural was detected in FM-001 in the old honey stage 1,and 5-hydroxymethylfurfural was detected in most samples in the old honey stage 2.[Conclusions]The pH and 5-hydroxymethylfurfural content of 12 batches of Jiulongteng honey met the requirements within 3 years of storage.There was no 5-hydroxymethylfurfural in Jiulongteng honey,but with the extension of storage time,the detection amount of 5-hydroxymethylfurfural increased significantly even if Jiulongteng honey was stored at low temperature.Therefore,5-hydroxymethylfurfural can be used as an important indicator of honey freshness.
