The authors regret to report some missing information in the synthetic reagents and associated changes of the paper.On page 511,the author information reads:“5.0 mmol of citric acid(C_(6)H_(8)O_(7)),5.0 mmol of ferri...The authors regret to report some missing information in the synthetic reagents and associated changes of the paper.On page 511,the author information reads:“5.0 mmol of citric acid(C_(6)H_(8)O_(7)),5.0 mmol of ferric chloride hexahydrate(FeCl_(3)·6H_(2)O),and 10.0 mmol of o-phenylenediamine(C_(6)H_(8)N_(2))were combined with 40 mL of deionized water and magnetically stirred until fully dissolved.”展开更多
Excessive accumulation of cadmium (Cd) impairs crop growth by inducing oxidative damage through the generation of reactive oxygen species (ROS). In this study, a biocompatible ferruginated carbon quantum dots (Fe-CQDs...Excessive accumulation of cadmium (Cd) impairs crop growth by inducing oxidative damage through the generation of reactive oxygen species (ROS). In this study, a biocompatible ferruginated carbon quantum dots (Fe-CQDs) nanozyme is developed to target ROS, thereby reducing oxidative damage and improving the absorption and transfer of Cd ions in wheat. Notably, Fe-CQDs exhibit multi-enzyme activities mimicking peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD), enabling effective neutralization of active species such as hydroxyl radicals (•OH), hydrogen peroxide (H_(2)O_(2)), and superoxide anions (O_(2)•^(-)). Importantly, root application of 10 mg L^(-1) Fe-CQDs alleviates Cd stress and promotes wheat growth in both hydroponic and soil cultures. Specifically, the levels of O_(2)•^(-), H_(2)O_(2), and malondialdehyde (MDA) in leaf tissues decrease, whereas the non-enzyme antioxidant, reduced glutathione (GSH), increases. Cell wall thickness in the Fe-CQDs-treated group is reduced by 42.4% compared with the Cd group. Moreover, Fe-CQDs enhance the expression of genes related to antioxidants, stress resistance, Cd detoxification, and nutrient transport. Transcriptomic and metabolomic analyses show that Fe-CQDs stimulate the production of flavonoids and regulate the activity of metal transporter genes (YSL, ABC, ZIP) to maintain ROS homeostasis. These findings highlight the potential of Fe-CQDs nanozyme platforms in mitigating oxidative damage and enhancing crop growth, offering new insights into the application of nanobiotechnology in agriculture.展开更多
Durian(Durio zibethinus Murr.),a prized tropical fruit native to Southeast Asia,is known for its unique texture and sweet-bitter aroma.To understand the primary and secondary metabolites influencing durian’s texture ...Durian(Durio zibethinus Murr.),a prized tropical fruit native to Southeast Asia,is known for its unique texture and sweet-bitter aroma.To understand the primary and secondary metabolites influencing durian’s texture and aroma,an integrative approach combining electronic nose and tongue analyses,transcriptomics,and metabolomics was first applied to three popular durian varieties:Musang King(MK),Monthong(MT),and Kan Yau(KY).The study identified 82 non-volatile and 95 volatile differentially expressed metabolites,with MK containing higher levels of terpenoids and sulfur compounds,while MT exhibited elevated esters and lipid-derived volatiles,contributing to its distinctive sweetness and aroma intensity.In contrast,KY was enriched in phenolic acids and amino acid derivatives,which may influence its bitterness and umami taste.Transcriptomic analysis revealed 12927 differentially expressed genes,with key metabolic pathways including carbon metabolism,amino acid biosynthesis,and lipid metabolism playing significant roles in distinguishing these varieties.Furthermore,network pharmacology analysis pinpointed 45 key non-volatile metabolites with potential health benefits,including flavonoids,lipids,and alkaloids,which were linked to anti-inflammatory,cardiovascular-protective,and neuroprotective properties.Notably,MK and KY contained bioactive compounds with potential anticancer effects,while MT was associated with metabolites beneficial for metabolic regulation.This study enhances our understanding of the genetic and metabolic basis underlying durian’s texture,aroma,and functional properties,providing a foundation for future breeding and functional food development.展开更多
Camellia flowers are an important oil source containing abundant phenolic compounds.The free and bound phenolic,flavonoid,and proanthocyanidin fractions and their antioxidant activity were investigated in Camellia ole...Camellia flowers are an important oil source containing abundant phenolic compounds.The free and bound phenolic,flavonoid,and proanthocyanidin fractions and their antioxidant activity were investigated in Camellia oleifera flowers and Camellia polyodonta flowers,stamens,and petals.Phenolic compounds in C.oleifera were studied using high-resolution liquid chromatography-mass spectrometry,which revealed 85 phenolics,including flavonols>ellagitannins>procyanidins>phenolic acids>flavanone>organic acids.Free fractions contributed more greatly to phenolic content and antioxidant capacity than bound fractions in all samples.The highest total phenolics(138.96 mg GAE/g DW),total flavonoids(423.20 mg RE/g DW),and total proanthocyanidins(61.08 mg PB2E/g DW)were obtained in the C.polyodonta flowers,which also exhibited the highest total antioxidant activity.In terms of individual phenolic components,C.polyodonta possessed the highest content of procyanidin B2,procyanidin B4,procyanidin C1,and epicatechin,while C.oleifera displayed the highest content of flavonoids such as quercitrin and afzelin,particularly in the petals.These results implied that Camellia flowers are important natural sources of active components for use in the food industry.展开更多
文摘The authors regret to report some missing information in the synthetic reagents and associated changes of the paper.On page 511,the author information reads:“5.0 mmol of citric acid(C_(6)H_(8)O_(7)),5.0 mmol of ferric chloride hexahydrate(FeCl_(3)·6H_(2)O),and 10.0 mmol of o-phenylenediamine(C_(6)H_(8)N_(2))were combined with 40 mL of deionized water and magnetically stirred until fully dissolved.”
基金supported by the National Natural Science Foundation of China (3237152 and 22104102)Natural Science Foundation of Sichuan Province (2025ZNSFSC0155)+1 种基金Open Project Program (SKL-KF202416) of State Key Laboratory of Crop Gene Exploration and Utilization in Southwest ChinaTwo-Way Support Programs of Sichuan Agricultural University (P202105)
文摘Excessive accumulation of cadmium (Cd) impairs crop growth by inducing oxidative damage through the generation of reactive oxygen species (ROS). In this study, a biocompatible ferruginated carbon quantum dots (Fe-CQDs) nanozyme is developed to target ROS, thereby reducing oxidative damage and improving the absorption and transfer of Cd ions in wheat. Notably, Fe-CQDs exhibit multi-enzyme activities mimicking peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD), enabling effective neutralization of active species such as hydroxyl radicals (•OH), hydrogen peroxide (H_(2)O_(2)), and superoxide anions (O_(2)•^(-)). Importantly, root application of 10 mg L^(-1) Fe-CQDs alleviates Cd stress and promotes wheat growth in both hydroponic and soil cultures. Specifically, the levels of O_(2)•^(-), H_(2)O_(2), and malondialdehyde (MDA) in leaf tissues decrease, whereas the non-enzyme antioxidant, reduced glutathione (GSH), increases. Cell wall thickness in the Fe-CQDs-treated group is reduced by 42.4% compared with the Cd group. Moreover, Fe-CQDs enhance the expression of genes related to antioxidants, stress resistance, Cd detoxification, and nutrient transport. Transcriptomic and metabolomic analyses show that Fe-CQDs stimulate the production of flavonoids and regulate the activity of metal transporter genes (YSL, ABC, ZIP) to maintain ROS homeostasis. These findings highlight the potential of Fe-CQDs nanozyme platforms in mitigating oxidative damage and enhancing crop growth, offering new insights into the application of nanobiotechnology in agriculture.
基金supported by the basic Scientific research business Expenses of Hainan academy of agricultural Sciences(ITH2024ZD02)Hainan academy of agricultural Sciences Program(HaaS2022JbGS01)the Hainan Provincial Key research and Development Project(ZDYF2024XDNY251).
文摘Durian(Durio zibethinus Murr.),a prized tropical fruit native to Southeast Asia,is known for its unique texture and sweet-bitter aroma.To understand the primary and secondary metabolites influencing durian’s texture and aroma,an integrative approach combining electronic nose and tongue analyses,transcriptomics,and metabolomics was first applied to three popular durian varieties:Musang King(MK),Monthong(MT),and Kan Yau(KY).The study identified 82 non-volatile and 95 volatile differentially expressed metabolites,with MK containing higher levels of terpenoids and sulfur compounds,while MT exhibited elevated esters and lipid-derived volatiles,contributing to its distinctive sweetness and aroma intensity.In contrast,KY was enriched in phenolic acids and amino acid derivatives,which may influence its bitterness and umami taste.Transcriptomic analysis revealed 12927 differentially expressed genes,with key metabolic pathways including carbon metabolism,amino acid biosynthesis,and lipid metabolism playing significant roles in distinguishing these varieties.Furthermore,network pharmacology analysis pinpointed 45 key non-volatile metabolites with potential health benefits,including flavonoids,lipids,and alkaloids,which were linked to anti-inflammatory,cardiovascular-protective,and neuroprotective properties.Notably,MK and KY contained bioactive compounds with potential anticancer effects,while MT was associated with metabolites beneficial for metabolic regulation.This study enhances our understanding of the genetic and metabolic basis underlying durian’s texture,aroma,and functional properties,providing a foundation for future breeding and functional food development.
基金supported by Supported by Sichuan Science and Technology Program(2020YFH0207)National Natural Science Foundation of China(31900294).
文摘Camellia flowers are an important oil source containing abundant phenolic compounds.The free and bound phenolic,flavonoid,and proanthocyanidin fractions and their antioxidant activity were investigated in Camellia oleifera flowers and Camellia polyodonta flowers,stamens,and petals.Phenolic compounds in C.oleifera were studied using high-resolution liquid chromatography-mass spectrometry,which revealed 85 phenolics,including flavonols>ellagitannins>procyanidins>phenolic acids>flavanone>organic acids.Free fractions contributed more greatly to phenolic content and antioxidant capacity than bound fractions in all samples.The highest total phenolics(138.96 mg GAE/g DW),total flavonoids(423.20 mg RE/g DW),and total proanthocyanidins(61.08 mg PB2E/g DW)were obtained in the C.polyodonta flowers,which also exhibited the highest total antioxidant activity.In terms of individual phenolic components,C.polyodonta possessed the highest content of procyanidin B2,procyanidin B4,procyanidin C1,and epicatechin,while C.oleifera displayed the highest content of flavonoids such as quercitrin and afzelin,particularly in the petals.These results implied that Camellia flowers are important natural sources of active components for use in the food industry.
