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萝卜RsbHLH116基因克隆与生物信息学分析
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作者 蔡文姝 刘德松 +5 位作者 鹿筱萱 赵世豪 卜昕宇 胡宗彩 韩童 李媛媛 《产业科技创新》 2025年第2期118-121,共4页
本文研究克隆了萝卜RsbHLH116基因CDS序列,分析显示其开放阅读框长912bp,编码303个氨基酸,分子量34.77kDa,等电点5.71,为酸性蛋白,且属亲水性不稳定蛋白。该蛋白含HLH保守结构域,无跨膜区和信号肽,亚细胞定位预测为细胞核。系统进化表... 本文研究克隆了萝卜RsbHLH116基因CDS序列,分析显示其开放阅读框长912bp,编码303个氨基酸,分子量34.77kDa,等电点5.71,为酸性蛋白,且属亲水性不稳定蛋白。该蛋白含HLH保守结构域,无跨膜区和信号肽,亚细胞定位预测为细胞核。系统进化表明其与十字花科植物同源性高,三级结构与甘蓝型油菜bHLH蛋白相似度达82%。启动子含光响应、昼夜节律等调控元件。本文的研究为解析萝卜绿肉性状调控机制提供了基因资源。 展开更多
关键词 萝卜 RsbHLH116 基因克隆 生物信息学分析
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Experimental demonstration of pyramidal neuron-like dynamics dominated by dendritic action potentials based on a VCSEL for all-optical XOR classification task 被引量:7
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作者 YAHUI ZHANG SHUIYING XIANG +4 位作者 XINGYU CAO shihao zhao XINGXING GUO AIJUN WEN YUE HAO 《Photonics Research》 SCIE EI CAS CSCD 2021年第6期1055-1061,共7页
We experimentally and numerically demonstrate an approach to optically reproduce a pyramidal neuron-like dynamics dominated by dendritic Ca^(2+) action potentials(dCaAPs)based on a vertical-cavity surface-emitting las... We experimentally and numerically demonstrate an approach to optically reproduce a pyramidal neuron-like dynamics dominated by dendritic Ca^(2+) action potentials(dCaAPs)based on a vertical-cavity surface-emitting laser(VCSEL)for the first time.The biological pyramidal neural dynamics dominated by dCaAPs indicates that the dendritic electrode evoked somatic spikes with current near threshold but failed to evoke(or evoked less)somatic spikes for higher current intensity.The emulating neuron-like dynamics is performed optically based on the injection locking,spiking dynamics,and damped oscillations in the optically injected VCSEL.In addition,the exclusive OR(XOR)classification task is examined in the VCSEL neuron equipped with the pyramidal neuronlike dynamics dominated by dCaAPs.Furthermore,a single spike or multiple periodic spikes are suggested to express the result of the XOR classification task for enhancing the processing rate or accuracy.The experimental and numerical results show that the XOR classification task is achieved successfully in the VCSEL neuron enabled to mimic the pyramidal neuron-like dynamics dominated by dCaAPs.This work reveals valuable pyramidal neuron-like dynamics in a VCSEL and offers a novel approach to solve XOR classification task with a fast and simple all-optical spiking neural network,and hence shows great potentials for future photonic spiking neural networks and photonic neuromorphic computing. 展开更多
关键词 optical DYNAMICS action
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Airflow field simulation model building and application for aerobic compost using CFD 被引量:1
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作者 Qiongyi Cheng Haibo Meng +5 位作者 Haibin Zhou Yujun Shen Hongsheng Cheng Jingtao Ding shihao zhao Shance Hou 《International Journal of Agricultural and Biological Engineering》 SCIE EI CAS 2021年第4期245-254,共10页
Composting is a suitable low-cost strategy to transform animal waste into a stabilized end product,and the airflow field in the pile affects the fermentation states.For piles in the aeration weak zone,anaerobic fermen... Composting is a suitable low-cost strategy to transform animal waste into a stabilized end product,and the airflow field in the pile affects the fermentation states.For piles in the aeration weak zone,anaerobic fermentation may happen as the lack of oxygen.While in practice,due to the extremely low speed and harsh condition,the airflow field was scarcely researched.This study adopted Computational Fluid Dynamics(CFD)to investigate the airflow field in the compost pile.A three-dimensional CFD model was built;the compost pile in this model was simplified as porous media;unstructured mesh was adopted to discretize the geometric model;standard k-εmodel and SIMPLE scheme were employed for numerical methods.The CFD model was validated with the field experiment in the cooling period.Then the model was applied to research the airflow field in the pile with increased pipe diameter.Results showed that it was feasible to investigate the airflow field in the pile with CFD.For boundary conditions in the CFD model,treating the compost pile as a homogeneous porous media is reasonable.The CFD model was successfully validated by the field measurement(the average relative difference between the simulated and measured air speed was within 10%).Airflow in the existing compost pile showed nonuniform distribution with stagnation and vortex zones;the turbulence intensities were 3.57 and 1.43 for the airflow in the pile and the reactor,respectively.When pipe diameter increased,the airflow distribution uniformity in the pile was improved significantly.With diameter raising to 0.04 m,the turbulence intensity decreased to 1.54 and 0.42 for the airflow in the pile and the reactor,respectively.The CFD model developed in this research can provide qualitative and quantitative analyses of the airflow field in compost piles,and can be a theoretical basis for the aeration system design. 展开更多
关键词 CFD model building COMPOST airflow field PILE
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