Antibacterial lysozyme hydrogels show attractive advantages in wound dressings due to their intrinsic antibacterial activity and excellent biochemical and mechanical properties.Unfortunately,the development of such hy...Antibacterial lysozyme hydrogels show attractive advantages in wound dressings due to their intrinsic antibacterial activity and excellent biochemical and mechanical properties.Unfortunately,the development of such hydrogels is still greatly limited due to the lack of universal gelation strategies.Herein,a universal gelation strategy between lysozyme-nanofiber(LZF)and inorganic salts is proposed for the first time to construct functional nanofibrous lysozyme-based hydrogels.In particular,divalent anions are found to universally drive LZF for the aggregation and transformation into three-dimensional nanofibrous network hydrogels via electrostatic interaction,and the key role of divalent anions in the gelation is further proved by molecular dynamics simulation.In addition,near-infrared light-mediated photothermal characteristics are endowed with LZF to enhance its inhibitory activity of multidrug-resistant bacteria by the skeleton modification with genipin to produce genipin-conjuagted LZF(GLZF).As a distinct application paradigm,the brilliant immunomemory MnSO_(4)-crosslinked GLZF hydrogel is constructed to sensitize the cGAS-STING pathway and skillfully establish an antibacterial immune microenvironment.It can excellently realize the anti-recurrence of diabetic wound infection via photo-enhanced bacterial killing and the cGAS-STING pathway.Thereby,it paves the way to employ the universal divalent anion-mediated gelation strategy for the future development of functional inorganic salt hybrid lysozyme hydrogels.展开更多
基金supported by the National Natural Science Foundation of China(22265004 and 22377018)the BAGUI Youth Elite Talent Training Project of Guangxi,the BAGUI Scholar Program of Guangxi,State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources(CMEMR2023-A03)+1 种基金the National College Students’Innovation and Entrepreneurship Training Program(S202310602089)the Innovation Project of Guangxi Graduate Education(YCBZ202352).
文摘Antibacterial lysozyme hydrogels show attractive advantages in wound dressings due to their intrinsic antibacterial activity and excellent biochemical and mechanical properties.Unfortunately,the development of such hydrogels is still greatly limited due to the lack of universal gelation strategies.Herein,a universal gelation strategy between lysozyme-nanofiber(LZF)and inorganic salts is proposed for the first time to construct functional nanofibrous lysozyme-based hydrogels.In particular,divalent anions are found to universally drive LZF for the aggregation and transformation into three-dimensional nanofibrous network hydrogels via electrostatic interaction,and the key role of divalent anions in the gelation is further proved by molecular dynamics simulation.In addition,near-infrared light-mediated photothermal characteristics are endowed with LZF to enhance its inhibitory activity of multidrug-resistant bacteria by the skeleton modification with genipin to produce genipin-conjuagted LZF(GLZF).As a distinct application paradigm,the brilliant immunomemory MnSO_(4)-crosslinked GLZF hydrogel is constructed to sensitize the cGAS-STING pathway and skillfully establish an antibacterial immune microenvironment.It can excellently realize the anti-recurrence of diabetic wound infection via photo-enhanced bacterial killing and the cGAS-STING pathway.Thereby,it paves the way to employ the universal divalent anion-mediated gelation strategy for the future development of functional inorganic salt hybrid lysozyme hydrogels.
