Lauraceae includes the genus Phoebe,and the family is linked to the evolution of magnoliids.We sequenced the genome of Phoebe bournei Nanmu.The assembled genome size was 989.19 Mb,with a contig N50 value of 2.05 Mb.A ...Lauraceae includes the genus Phoebe,and the family is linked to the evolution of magnoliids.We sequenced the genome of Phoebe bournei Nanmu.The assembled genome size was 989.19 Mb,with a contig N50 value of 2.05 Mb.A total of 28,198 protein-coding genes were annotated in P.bournei.Whole-genome duplication(WGD)analysis showed that Lauraceae has experienced two WGD events;the older WGD event occurred just before the divergence of Lauraceae and Magnoliales,and the more recent WGD was shared by all lineages of Lauraceae.The phylogenetic tree showed that magnoliids form a sister clade to monocots and eudicots.We also identified 63 MADS-box genes,including AGL12-like genes that may be related to the regulation of P.bournei roots and FIN219-like genes encoding GH3 proteins,which are involved in photomorphogenesis.SAUR50-like genes involved in light signal-mediated pedicel or stem development were also identified.Four ATMYB46-and three PtrEPSP-homologous genes related to lignin biosynthesis were identified.These genes may be associated with the formation of straight trunks in P.bournei.Overall,the P.bournei reference genome provides insight into the origin,evolution,and diversification of Phoebe and other magnoliids.展开更多
After online publication of the article 1 the author noticed minor typo in Fig.4c.CeSEP1/2/3/4 should be read as CeSEP1/3/4.Revised figure supplied during the proof correction was inadvertently not reproduced in the f...After online publication of the article 1 the author noticed minor typo in Fig.4c.CeSEP1/2/3/4 should be read as CeSEP1/3/4.Revised figure supplied during the proof correction was inadvertently not reproduced in the final version.In addition,the affiliation numbers of the authors revised in the original article during proof correction,but not reflected in the title page in Supplementary Information.展开更多
The marvelously diverse Orchidaceae constitutes the largest family of angiosperms.The genus Cymbidium in Orchidaceae is well known for its unique vegetation,floral morphology,and flower scent traits.Here,a chromosomes...The marvelously diverse Orchidaceae constitutes the largest family of angiosperms.The genus Cymbidium in Orchidaceae is well known for its unique vegetation,floral morphology,and flower scent traits.Here,a chromosomescale assembly of the genome of Cymbidium ensifolium(Jianlan)is presented.Comparative genomic analysis showed that C.ensifolium has experienced two whole-genome duplication(WGD)events,the most recent of which was shared by all orchids,while the older event was the t event shared by most monocots.The results of MADS-box genes analysis provided support for establishing a unique gene model of orchid flower development regulation,and flower shape mutations in C.ensifolium were shown to be associated with the abnormal expression of MADS-box genes.The most abundant floral scent components identi fied included methyl jasmonate,acacia alcohol and linalool,and the genes involved in the floral scent component network of C.ensifolium were determined.Furthermore,the decreased expression of photosynthesis-antennae and photosynthesis metabolic pathway genes in leaves was shown to result in colorful striped leaves,while the increased expression of MADS-box genes in leaves led to perianth-like leaves.Our results provide fundamental insights into orchid evolution and diversi fication.展开更多
基金funded by the Project of Forestry Peak Discipline at Fujian Agriculture and Forestry University,China(118/712018007)the Science and Technology Innovation Special Fund at Fujian Agriculture and Forestry University(118/KF2015088)the 6th Project of Forest Seeding Breaking in Fujian Province([2–19]357).
文摘Lauraceae includes the genus Phoebe,and the family is linked to the evolution of magnoliids.We sequenced the genome of Phoebe bournei Nanmu.The assembled genome size was 989.19 Mb,with a contig N50 value of 2.05 Mb.A total of 28,198 protein-coding genes were annotated in P.bournei.Whole-genome duplication(WGD)analysis showed that Lauraceae has experienced two WGD events;the older WGD event occurred just before the divergence of Lauraceae and Magnoliales,and the more recent WGD was shared by all lineages of Lauraceae.The phylogenetic tree showed that magnoliids form a sister clade to monocots and eudicots.We also identified 63 MADS-box genes,including AGL12-like genes that may be related to the regulation of P.bournei roots and FIN219-like genes encoding GH3 proteins,which are involved in photomorphogenesis.SAUR50-like genes involved in light signal-mediated pedicel or stem development were also identified.Four ATMYB46-and three PtrEPSP-homologous genes related to lignin biosynthesis were identified.These genes may be associated with the formation of straight trunks in P.bournei.Overall,the P.bournei reference genome provides insight into the origin,evolution,and diversification of Phoebe and other magnoliids.
文摘After online publication of the article 1 the author noticed minor typo in Fig.4c.CeSEP1/2/3/4 should be read as CeSEP1/3/4.Revised figure supplied during the proof correction was inadvertently not reproduced in the final version.In addition,the affiliation numbers of the authors revised in the original article during proof correction,but not reflected in the title page in Supplementary Information.
基金The authors acknowledge support from the National Key Research and Development Program of China(no.2019YFD1000400)the National Natural Science Foundation of China(no.31700618)+2 种基金the Outstanding Young Scientific Research Talent Project of Fujian Agriculture and Forestry University(no.xjq201910)the Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilizati on Construction Funds(nos.115/118990050,115/KJG18016A)the Furopean Research Council(ERC)under the Furopean Union's Horizon 2020 research and innovation program(grant agreement No 833522).
文摘The marvelously diverse Orchidaceae constitutes the largest family of angiosperms.The genus Cymbidium in Orchidaceae is well known for its unique vegetation,floral morphology,and flower scent traits.Here,a chromosomescale assembly of the genome of Cymbidium ensifolium(Jianlan)is presented.Comparative genomic analysis showed that C.ensifolium has experienced two whole-genome duplication(WGD)events,the most recent of which was shared by all orchids,while the older event was the t event shared by most monocots.The results of MADS-box genes analysis provided support for establishing a unique gene model of orchid flower development regulation,and flower shape mutations in C.ensifolium were shown to be associated with the abnormal expression of MADS-box genes.The most abundant floral scent components identi fied included methyl jasmonate,acacia alcohol and linalool,and the genes involved in the floral scent component network of C.ensifolium were determined.Furthermore,the decreased expression of photosynthesis-antennae and photosynthesis metabolic pathway genes in leaves was shown to result in colorful striped leaves,while the increased expression of MADS-box genes in leaves led to perianth-like leaves.Our results provide fundamental insights into orchid evolution and diversi fication.
