It has been demonstrated thatβ-elemene could protect against carbon tetrachloride(CCl_(4))-induced liver fibrosis in our laboratory work,and the aim of this paper is to reveal the protective mechanisms ofβ-elemene.T...It has been demonstrated thatβ-elemene could protect against carbon tetrachloride(CCl_(4))-induced liver fibrosis in our laboratory work,and the aim of this paper is to reveal the protective mechanisms ofβ-elemene.The hepatic fibrosis experimental model was induced by the hypodermical injection of CCl_(4) in Wistar male rats.β-elemene was intraperitoneally administered into rats for 8 weeks(0.1 mL/100 g bodyweight per day),and plasma endotoxin content was assayed by biochemistry.The serum TNF-αlevel was detected using radioactive immunity.CD14 expression in rat livers was measured by immunohistochemistry and Western blot.The results showed thatβ-elemene can downregulate the levels of plasma endotoxins,serum TNF-α,and hepatic CD14 expression in rats with liver fibrosis.β-elemene plays an important role in downregulating the lipopolysaccharide signal transduction pathway,a significant pathway in hepatic fibrosis development.展开更多
文摘It has been demonstrated thatβ-elemene could protect against carbon tetrachloride(CCl_(4))-induced liver fibrosis in our laboratory work,and the aim of this paper is to reveal the protective mechanisms ofβ-elemene.The hepatic fibrosis experimental model was induced by the hypodermical injection of CCl_(4) in Wistar male rats.β-elemene was intraperitoneally administered into rats for 8 weeks(0.1 mL/100 g bodyweight per day),and plasma endotoxin content was assayed by biochemistry.The serum TNF-αlevel was detected using radioactive immunity.CD14 expression in rat livers was measured by immunohistochemistry and Western blot.The results showed thatβ-elemene can downregulate the levels of plasma endotoxins,serum TNF-α,and hepatic CD14 expression in rats with liver fibrosis.β-elemene plays an important role in downregulating the lipopolysaccharide signal transduction pathway,a significant pathway in hepatic fibrosis development.
