Objective Histamine N-methyltransferase(HNMT)is involved primarily in histamine metabolism,but emerging evidence suggests its potential role in cancer progression.This study investigated the role of HNMT in nasopharyn...Objective Histamine N-methyltransferase(HNMT)is involved primarily in histamine metabolism,but emerging evidence suggests its potential role in cancer progression.This study investigated the role of HNMT in nasopharyngeal carcinoma(NPC)and its impact on interferon(IFN)signaling,thioredoxin-interacting protein(TXNIP),and p53 tumor suppressor pathways.Methods HNMT expression in NPC tissues and cell lines was analyzed via qPCR and Western blotting.Functional assays,including cell proliferation,migration,invasion,and apoptosis,were performed after HNMT knockdown or overexpression.Transcriptomic sequencing was used to identify differentially expressed genes(DEGs).In addition,we examined the relationship between HNMT and the IFN/TXNIP/p53 axis via rescue experiments in vitro and in vivo models via qPCR and Western blotting.Results HNMT knockdown reduced cell proliferation,migration,and invasion,and promoted apoptosis in NPC tissues and cell lines.TXNIP was the most significantly upregulated gene following HNMT knockdown.Inhibition of the IFN pathway reversed these effects,confirming the role of HNMT in downregulating the IFN/TXNIP/p53 pathway.An in vivo study revealed that HNMT overexpression correlated with reduced expression of TXNIP and p53 in NCG mice.Conclusion In NPC,HNMT promotes tumor growth and progression by inhibiting the IFN/TXNIP/p53 axis.These findings suggest that targeting the HNMT axis or restoring its function could provide new therapeutic strategies for NPC.展开更多
The complex interplay of magnetic interactions at the yttrium iron garnet(YIG)/ferromagnet interface is important for spintronic and magnonic devices.In this study,we present a comprehensive investigation of the inter...The complex interplay of magnetic interactions at the yttrium iron garnet(YIG)/ferromagnet interface is important for spintronic and magnonic devices.In this study,we present a comprehensive investigation of the interlayer coupling and switching mechanisms in YIG/Py(permalloy)heterostructures based on gadolinium gallium garnet(GGG)and SiO_(2)substrates.We observe antiferromagnetic interlayer coupling between Py and YIG on SiO_(2)substrates,whereas ferromagnetic interlayer coupling is observed on GGG substrates.Using polarized neutron reflectometry with depth-and elementresolved measurements,we obtain an in-depth understanding of the magnetic interactions between the YIG and Py layers.We demonstrate that polycrystalline YIG gives rise to antiferromagnetic interlayer coupling.This work provides valuable insights into designing and controlling magnetic coupling in hybrid structures for spintronic applications.展开更多
Photocatalysis driven by near-infrared（NIR）light is of scientific and technological interest for ex-ploiting solar energy.In this study,we demonstrate a facile hydrothermal process to synthesize core-shell nanoparti...Photocatalysis driven by near-infrared（NIR）light is of scientific and technological interest for ex-ploiting solar energy.In this study,we demonstrate a facile hydrothermal process to synthesize core-shell nanoparticles combining upconversion nanoparticles（UCNPs）and alloyed ZnxCwhich can be excited using NIR or visible light.Morphologies,phase,and chemical composition have been investigated using field-emission scanning electron microscopy,transmission electron mi-croscopy,X-ray diffraction analysis,and atomic absorption spectroscopy.Moreover,we found that amorphous TiO2 layers existing in the final samples play an important role in formation ofyolk-shell nanoparticles,which bind the as-prepared ZnxCnanoparticlescan be tuna-ble by adjusting the amount of the Cd and Zn source compounds.The photochemical reduction of Cr（Ⅵ）in water has been performed to study the photocatalytic performance under irradiation by NIR light or a simulated solar light,showing efficient photoreduction and Cr（Ⅵ）removal over the/TiO2 yolk-shell nanoparticles.The as-prepared UCNPs@ZnxC/TiO2 nanoparticles show excellent production of hydroxyl radicals,which are responsible for the photochemical reduction of Cr（Ⅵ）to Cr（Ⅲ）.This study will provide an alternative strategy for en-vironmental wastewater treatment,making full use of solar energy.展开更多
Research on recycling waste Printed Circuit Boards(PCB) is at the forefront of preventing environmental pollution and finding ways to recycle resources.The Tapered Column Separation Bed(TCSB) is invented aiming at dis...Research on recycling waste Printed Circuit Boards(PCB) is at the forefront of preventing environmental pollution and finding ways to recycle resources.The Tapered Column Separation Bed(TCSB) is invented aiming at disposing the problem that fine particles of waste printed circuit boards cannot be separated efficiently so as to obtain further insight about the underlying mechanisms and demonstrate the separation feasibility in the tapered column separation bed.In this work,a Computational Fluid Dynamics(CFD) coupled with Discrete Element Method(DEM) model for two-phase flow has been extended to simulate the fluid-solid flow in the tapered column separation bed.Its validity is demonstrated by its successful capturing the key features of particles' flow pattern,velocity,the pressure distribution,the axial position with time and axial force for particles with different densities.Simulation results show that the plastic particles and resin particles become overflow,while copper particles,iron particles and aluminum particles successively become underflow,with a discharge water flow rate of 1 m^3/h,an obliquity of 30°.The simulated results agree reasonably well with the experimental observation.Using this equipment to separate waste PCBs is feasible,theoretically.展开更多
Pressure fluctuations contribute to the instability of separation process in air dense medium fluidized bed, which provides a high motivation for further study of underlying mechanisms. Reasons for generation and prop...Pressure fluctuations contribute to the instability of separation process in air dense medium fluidized bed, which provides a high motivation for further study of underlying mechanisms. Reasons for generation and propagation of pressure fluctuations in the air dense medium fluidized bed have been discussed.Drift rate and collision rate of particles were employed to deduce the correlation between voidage and pressure fluctuations. Simultaneously, a dynamic pressure fluctuation measuring and analysis system was established. Based on frequency domain analysis and wavelet analysis, collected signals were disassembled and analyzed. Results show gradually intensive motion of particles increases magnitudes of signal components with lower frequencies. As a result of violent particle motion, the magnitude of real pressure signal's frequency experienced an increase as air velocity increased moderately. Wavelet analysis keeps edge features of the real signal and eliminates the noise efficaciously. The frequency of denoised signal is closed to that of pressure signal identified in frequency domain analysis.展开更多
基金supported by grants from Science Research Foundation of Yunnan Education Bureau(No.2023Y0631)Yunnan Provincial Science and Technology Project(No.202303AP140005).
文摘Objective Histamine N-methyltransferase(HNMT)is involved primarily in histamine metabolism,but emerging evidence suggests its potential role in cancer progression.This study investigated the role of HNMT in nasopharyngeal carcinoma(NPC)and its impact on interferon(IFN)signaling,thioredoxin-interacting protein(TXNIP),and p53 tumor suppressor pathways.Methods HNMT expression in NPC tissues and cell lines was analyzed via qPCR and Western blotting.Functional assays,including cell proliferation,migration,invasion,and apoptosis,were performed after HNMT knockdown or overexpression.Transcriptomic sequencing was used to identify differentially expressed genes(DEGs).In addition,we examined the relationship between HNMT and the IFN/TXNIP/p53 axis via rescue experiments in vitro and in vivo models via qPCR and Western blotting.Results HNMT knockdown reduced cell proliferation,migration,and invasion,and promoted apoptosis in NPC tissues and cell lines.TXNIP was the most significantly upregulated gene following HNMT knockdown.Inhibition of the IFN pathway reversed these effects,confirming the role of HNMT in downregulating the IFN/TXNIP/p53 pathway.An in vivo study revealed that HNMT overexpression correlated with reduced expression of TXNIP and p53 in NCG mice.Conclusion In NPC,HNMT promotes tumor growth and progression by inhibiting the IFN/TXNIP/p53 axis.These findings suggest that targeting the HNMT axis or restoring its function could provide new therapeutic strategies for NPC.
基金supported by the National Key Basic Research Program of China(Grant Nos.2021YFA1400300 and 2023YFA1610400)the National Natural Science Foundation of China(Grant Nos.12204268,52371169,52130103,and U22A20263)+1 种基金the Guangdong Basic and Applied Basic Research Foundation(Grant No.2023B1515120015)the Open Research Fund of Songshan Lake Materials Laboratory(Grant No.2022SLABFN13)。
文摘The complex interplay of magnetic interactions at the yttrium iron garnet(YIG)/ferromagnet interface is important for spintronic and magnonic devices.In this study,we present a comprehensive investigation of the interlayer coupling and switching mechanisms in YIG/Py(permalloy)heterostructures based on gadolinium gallium garnet(GGG)and SiO_(2)substrates.We observe antiferromagnetic interlayer coupling between Py and YIG on SiO_(2)substrates,whereas ferromagnetic interlayer coupling is observed on GGG substrates.Using polarized neutron reflectometry with depth-and elementresolved measurements,we obtain an in-depth understanding of the magnetic interactions between the YIG and Py layers.We demonstrate that polycrystalline YIG gives rise to antiferromagnetic interlayer coupling.This work provides valuable insights into designing and controlling magnetic coupling in hybrid structures for spintronic applications.
基金supported by the National Natural Science Foundation of China (21471043, 51603059, 31501576)~~
文摘Photocatalysis driven by near-infrared（NIR）light is of scientific and technological interest for ex-ploiting solar energy.In this study,we demonstrate a facile hydrothermal process to synthesize core-shell nanoparticles combining upconversion nanoparticles（UCNPs）and alloyed ZnxCwhich can be excited using NIR or visible light.Morphologies,phase,and chemical composition have been investigated using field-emission scanning electron microscopy,transmission electron mi-croscopy,X-ray diffraction analysis,and atomic absorption spectroscopy.Moreover,we found that amorphous TiO2 layers existing in the final samples play an important role in formation ofyolk-shell nanoparticles,which bind the as-prepared ZnxCnanoparticlescan be tuna-ble by adjusting the amount of the Cd and Zn source compounds.The photochemical reduction of Cr（Ⅵ）in water has been performed to study the photocatalytic performance under irradiation by NIR light or a simulated solar light,showing efficient photoreduction and Cr（Ⅵ）removal over the/TiO2 yolk-shell nanoparticles.The as-prepared UCNPs@ZnxC/TiO2 nanoparticles show excellent production of hydroxyl radicals,which are responsible for the photochemical reduction of Cr（Ⅵ）to Cr（Ⅲ）.This study will provide an alternative strategy for en-vironmental wastewater treatment,making full use of solar energy.
基金the National Natural Science Foundation of China(81672520,81870484,61975172,and 82001874,used for procurement of materials and labor cost)Science and Technology Planning Project of Zhejiang(2019C03089,used for procurement of materials)Zhejiang Provincial Natural Science Foundation of China(LR17F050001,used for procurement of materials).
基金the National Key Basic Research Program of China(No.2012CB214904)the National Natural Science Foundation of China for Innovative Research Group(No.51221462)+2 种基金the National Natural Science Foundation of China(Nos.51304196,51134022,and 51174203)the Natural Science Foundation of Jiangsu Province of China(No. BK2012136)the Specialized Research Fund for the Doctoral Program of Higher Education(No.20120095130001)
文摘Research on recycling waste Printed Circuit Boards(PCB) is at the forefront of preventing environmental pollution and finding ways to recycle resources.The Tapered Column Separation Bed(TCSB) is invented aiming at disposing the problem that fine particles of waste printed circuit boards cannot be separated efficiently so as to obtain further insight about the underlying mechanisms and demonstrate the separation feasibility in the tapered column separation bed.In this work,a Computational Fluid Dynamics(CFD) coupled with Discrete Element Method(DEM) model for two-phase flow has been extended to simulate the fluid-solid flow in the tapered column separation bed.Its validity is demonstrated by its successful capturing the key features of particles' flow pattern,velocity,the pressure distribution,the axial position with time and axial force for particles with different densities.Simulation results show that the plastic particles and resin particles become overflow,while copper particles,iron particles and aluminum particles successively become underflow,with a discharge water flow rate of 1 m^3/h,an obliquity of 30°.The simulated results agree reasonably well with the experimental observation.Using this equipment to separate waste PCBs is feasible,theoretically.
基金support by the Natural Science Foundation of Jiangsu Province of China (No. BK20160266)the National Natural Science Foundation of China (Nos. 51704287 and U1508210)the Priority Academic Program Development of Jiangsu Higher Education Institutions of China
文摘Pressure fluctuations contribute to the instability of separation process in air dense medium fluidized bed, which provides a high motivation for further study of underlying mechanisms. Reasons for generation and propagation of pressure fluctuations in the air dense medium fluidized bed have been discussed.Drift rate and collision rate of particles were employed to deduce the correlation between voidage and pressure fluctuations. Simultaneously, a dynamic pressure fluctuation measuring and analysis system was established. Based on frequency domain analysis and wavelet analysis, collected signals were disassembled and analyzed. Results show gradually intensive motion of particles increases magnitudes of signal components with lower frequencies. As a result of violent particle motion, the magnitude of real pressure signal's frequency experienced an increase as air velocity increased moderately. Wavelet analysis keeps edge features of the real signal and eliminates the noise efficaciously. The frequency of denoised signal is closed to that of pressure signal identified in frequency domain analysis.
