目的探讨过表达中期因子(MK)增强SMMC 7721细胞对5-氟尿嘧啶(5-Fu)耐药及其可能的机制。方法将SMMC 7721细胞转染pIRES2-EGFP-MK重组质粒过表达,采用实时荧光定量PCR(qPCR)和蛋白质印迹法(WB)检测MK mRNA和蛋白的表达。5-Fu处理后,采用...目的探讨过表达中期因子(MK)增强SMMC 7721细胞对5-氟尿嘧啶(5-Fu)耐药及其可能的机制。方法将SMMC 7721细胞转染pIRES2-EGFP-MK重组质粒过表达,采用实时荧光定量PCR(qPCR)和蛋白质印迹法(WB)检测MK mRNA和蛋白的表达。5-Fu处理后,采用MTT法检测SMMC 7721细胞对5-Fu的耐药。采用WB法检测磷酸化磷脂酰肌醇3-激酶(p-PI3K)、磷酸化蛋白激酶B(p-Akt)和NF-κB及Bcl-2、survivin、caspase-3的表达。结果将SMMC 7721细胞转染pIRES2-EGFP-MK重组质粒过表达后,MK mRNA和蛋白的表达增加,ConC组细胞对5-Fu的IC50显著高于Con-A组、Con-B组[(27.36±4.31)mg/L vs (4.57±0.34)mg/L,(4.96±0.46)mg/L],差异具有统计学意义(P<0.05)。此外,与Con-A组、Con-B组比较,Con-C组细胞p-PI3K(0.66±0.04 vs 0.35±0.03,0.57±0.03)、p-Akt (0.31±0.02 vs 0.17±0.02、0.25±0.02)、NF-κB (0.63±0.05 vs 0.27±0.02,0.53±0.04)、Bcl-2(0.42±0.04 vs 0.13±0.01, 0.38±0.04)、survivin(0.58±0.04 vs 0.18±0.02,0.51±0.04)呈现高表达,caspase-3(0.41±0.04vs 0.88±0.06,0.43±0.03)呈现低表达(P<0.05)。结论过表达MK可增强SMMC 7721细胞对5-Fu耐药,其机制可能与激活PI3K/AKT信号通路有关。展开更多
According to Ouyang potential temperature V-3θ charts, a rare autumn rainstorm in Xiamen on November 18, 2011 was compared with a spring rainstorm on March 29, 2005 firstly, and then the main characteristics of the a...According to Ouyang potential temperature V-3θ charts, a rare autumn rainstorm in Xiamen on November 18, 2011 was compared with a spring rainstorm on March 29, 2005 firstly, and then the main characteristics of the autumn rainstorm were analyzed based on wind profiler data of Doppler weather radar. The results showed that ultra-low temperature on the top of the troposphere was more obvious in autumn than that in spring; clockwise tumble effect was distinct and warm and wet layer reached 300 hPa in both spring and autumn. Clockwise tumble effect appeared in various forms in autumn and spring. That is, the wind blew from the east at low altitudes and the west at high altitudes before the autumn rainstorm oc- curred; when the maximum wind speed appeared at 500 hPa, heavy rainfall occurred. During the spring rainstorm process, wind direction was con- sistent at each level and wind speed increased with the rise of altitude during early days. Afterwards, the wind blew from the east at low altitudes and the west at high altitudes. During the autumn rainstorm process, the heavy rainfall at the first stage was closely related to thickness of the 20 m/s wind speed layer at 3 -5 km. If the thickness declined sharply when the 20 m/s wind speed layer expended downwards, heavy rainfall would occur soon afterwards. Moreover, the precipitation intensity weakened immediately as the 20 m/s wind speed layer began to be stable. The heavy rainfall at the second stage was closely related to thickness of the 24 m/s wind speed layer, and the appearance, development and weakening of the 24 m/s wind speed layer foreboded the occurrence of heavy rainfall. Moreover, the thickness of the 24 m/s wind speed layer decreased to the minimum, and then heavy rainfall would appear soon afterwards. Hereafter, the thickness increased and then decreased again.展开更多
文摘目的探讨过表达中期因子(MK)增强SMMC 7721细胞对5-氟尿嘧啶(5-Fu)耐药及其可能的机制。方法将SMMC 7721细胞转染pIRES2-EGFP-MK重组质粒过表达,采用实时荧光定量PCR(qPCR)和蛋白质印迹法(WB)检测MK mRNA和蛋白的表达。5-Fu处理后,采用MTT法检测SMMC 7721细胞对5-Fu的耐药。采用WB法检测磷酸化磷脂酰肌醇3-激酶(p-PI3K)、磷酸化蛋白激酶B(p-Akt)和NF-κB及Bcl-2、survivin、caspase-3的表达。结果将SMMC 7721细胞转染pIRES2-EGFP-MK重组质粒过表达后,MK mRNA和蛋白的表达增加,ConC组细胞对5-Fu的IC50显著高于Con-A组、Con-B组[(27.36±4.31)mg/L vs (4.57±0.34)mg/L,(4.96±0.46)mg/L],差异具有统计学意义(P<0.05)。此外,与Con-A组、Con-B组比较,Con-C组细胞p-PI3K(0.66±0.04 vs 0.35±0.03,0.57±0.03)、p-Akt (0.31±0.02 vs 0.17±0.02、0.25±0.02)、NF-κB (0.63±0.05 vs 0.27±0.02,0.53±0.04)、Bcl-2(0.42±0.04 vs 0.13±0.01, 0.38±0.04)、survivin(0.58±0.04 vs 0.18±0.02,0.51±0.04)呈现高表达,caspase-3(0.41±0.04vs 0.88±0.06,0.43±0.03)呈现低表达(P<0.05)。结论过表达MK可增强SMMC 7721细胞对5-Fu耐药,其机制可能与激活PI3K/AKT信号通路有关。
文摘According to Ouyang potential temperature V-3θ charts, a rare autumn rainstorm in Xiamen on November 18, 2011 was compared with a spring rainstorm on March 29, 2005 firstly, and then the main characteristics of the autumn rainstorm were analyzed based on wind profiler data of Doppler weather radar. The results showed that ultra-low temperature on the top of the troposphere was more obvious in autumn than that in spring; clockwise tumble effect was distinct and warm and wet layer reached 300 hPa in both spring and autumn. Clockwise tumble effect appeared in various forms in autumn and spring. That is, the wind blew from the east at low altitudes and the west at high altitudes before the autumn rainstorm oc- curred; when the maximum wind speed appeared at 500 hPa, heavy rainfall occurred. During the spring rainstorm process, wind direction was con- sistent at each level and wind speed increased with the rise of altitude during early days. Afterwards, the wind blew from the east at low altitudes and the west at high altitudes. During the autumn rainstorm process, the heavy rainfall at the first stage was closely related to thickness of the 20 m/s wind speed layer at 3 -5 km. If the thickness declined sharply when the 20 m/s wind speed layer expended downwards, heavy rainfall would occur soon afterwards. Moreover, the precipitation intensity weakened immediately as the 20 m/s wind speed layer began to be stable. The heavy rainfall at the second stage was closely related to thickness of the 24 m/s wind speed layer, and the appearance, development and weakening of the 24 m/s wind speed layer foreboded the occurrence of heavy rainfall. Moreover, the thickness of the 24 m/s wind speed layer decreased to the minimum, and then heavy rainfall would appear soon afterwards. Hereafter, the thickness increased and then decreased again.
