Background:Black ginseng is a unique processed product of ginseng,a source of health food,medicine and modern cosmetics.Purpose:The purpose of this study was to develop a new application for preventing inflammatory ag...Background:Black ginseng is a unique processed product of ginseng,a source of health food,medicine and modern cosmetics.Purpose:The purpose of this study was to develop a new application for preventing inflammatory aging.Method:To investigate the effects and mechanisms of action of black ginseng extract(BGE)on inflammatory aging,models of inflammatory aging were established in human foreskin fibroblasts cells and reconstructed 3D skin model.Result:The results showed that MMP-1 expression in human foreskin fibroblasts was reduced at a concentration of 4μg/mL BGE.At a concentration of 0.1%,BGE not only inhibited the secretion of PGE 2 in reconstructed 3D skin model damaged by SDS but also promoted the expression of TIMP-1.Furthermore,the composition of three rare ginsenosides in the BGE and their binding affinity to target proteins associated with inflammatory aging were analyzed using high-performance liquid chromatography(HPLC)and molecular docking technique.Conclusion:BGE has potential application value in alleviating skin inflammatory aging.展开更多
文摘Background:Black ginseng is a unique processed product of ginseng,a source of health food,medicine and modern cosmetics.Purpose:The purpose of this study was to develop a new application for preventing inflammatory aging.Method:To investigate the effects and mechanisms of action of black ginseng extract(BGE)on inflammatory aging,models of inflammatory aging were established in human foreskin fibroblasts cells and reconstructed 3D skin model.Result:The results showed that MMP-1 expression in human foreskin fibroblasts was reduced at a concentration of 4μg/mL BGE.At a concentration of 0.1%,BGE not only inhibited the secretion of PGE 2 in reconstructed 3D skin model damaged by SDS but also promoted the expression of TIMP-1.Furthermore,the composition of three rare ginsenosides in the BGE and their binding affinity to target proteins associated with inflammatory aging were analyzed using high-performance liquid chromatography(HPLC)and molecular docking technique.Conclusion:BGE has potential application value in alleviating skin inflammatory aging.
