In the past 30 years,the small baseline subset(SBAS)InSAR time-series technique has emerged as an essential tool for measuring slow surface displacement and estimating geophysical parameters.Because of its ability to ...In the past 30 years,the small baseline subset(SBAS)InSAR time-series technique has emerged as an essential tool for measuring slow surface displacement and estimating geophysical parameters.Because of its ability to monitor large-scale deformation with millimeter accuracy,the SBAS method has been widely used in various geodetic fields,such as ground subsidence,landslides,and seismic activity.The obtained long-term time-series cumulative deformation is vital for studying the deformation mecha-nism.This article reviews the algorithms,applications,and challenges of the SBAS method.First,we recall the fundamental principle and analyze the shortcomings of the traditional SBAS algorithm,which provides a basic framework for the following improved time series methods.Second,we classify the current improved SBAS techniques from different perspectives:solving the ill-posed equation,increasing the density of high-coherence points,improving the accuracy of monitoring deformation and measuring the multi-dimensional deformation.Third,we summarize the application of the SBAS method in monitoring ground subsidence,permafrost degradation,glacier movement,volcanic activity,landslides,and seismic activity.Finally,we discuss the difficulties faced by the SBAS method and explore its future development direction.展开更多
The well understanding of interaction forces between single dispersed droplets is crucial to the understanding of emulsion stabilization mechanism.Recently,many studies have reported the direct quantitative measuremen...The well understanding of interaction forces between single dispersed droplets is crucial to the understanding of emulsion stabilization mechanism.Recently,many studies have reported the direct quantitative measurements of interaction forces between 20-200μm single droplet coated polymers by atomic force microscope(AFM).These studies have revealed many important results about the relationship of the interaction forces and the droplet deformation.However,these studies of the quantitative relationship between the measured interaction forces and the separation distance of the front end of the droplet have rarely been reported.Optical tweezer instrument can make it possible to establish the quantitative relationship between the measured force and the separation distance of the front end of the droplet,which will make better understanding of the interaction mechanisms between droplets.Due to the differences of the measuring mechanism between atomic force microscopy(AFM)and optical tweezers,the theory model of AFM measurements cannot be fitted with the force measurement by optical tweezers.We have made an exhaustive comparison of the measuring differences between AFM and optical tweezer instrument in this work.Moreover,we built a numerical model to derive the repulsive pressure through the measured force curve in order to quantify the measured force of two micron-sized oil droplet coated polymers by optical tweezers.Furthermore,the novel method can be extended to other micron-sized emulsion systems,and these findings will be a vital progress on quantitative force measurements between micron-sized droplets.展开更多
Ionic thermoelectrics(i-TE) possesses great potential in powering distributed electronics because it can generate thermopower up to tens of millivolts per Kelvin. However,as ions cannot enter external circuit, the uti...Ionic thermoelectrics(i-TE) possesses great potential in powering distributed electronics because it can generate thermopower up to tens of millivolts per Kelvin. However,as ions cannot enter external circuit, the utilization of i-TE is currently based on capacitive charge/discharge, which results in discontinuous working mode and low energy density. Here,we introduce an ion–electron thermoelectric synergistic(IETS)effect by utilizing an ion–electron conductor. Electrons/holes can drift under the electric field generated by thermodiffusion of ions, thus converting the ionic current into electrical current that can pass through the external circuit. Due to the IETS effect, i-TE is able to operate continuously for over 3000 min.Moreover, our i-TE exhibits a thermopower of 32.7 mV K^(-1) and an energy density of 553.9 J m^(-2), which is more than 6.9 times of the highest reported value. Consequently, direct powering of electronics is achieved with i-TE. This work provides a novel strategy for the design of high-performance i-TE materials.展开更多
Thimerosal has been widely used as a preservative in drug and vaccine products for decades.Due to the strong propensity to modify thiols in proteins,conformational changes could occur due to covalent bond formation be...Thimerosal has been widely used as a preservative in drug and vaccine products for decades.Due to the strong propensity to modify thiols in proteins,conformational changes could occur due to covalent bond formation between ethylmercury(a degradant of thimerosal)and thiols.Such a conformational change could lead to partial or even complete loss of desirable protein function.This study aims to investigate the effects of thimerosal on the capsid stability and antigenicity of recombinant human papillomavirus(HPV)18 virus-like particles(VLPs).Dramatic destabilization of the recombinant viral capsid upon thimerosal treatment was observed.Such a negative effect on the thermal stability of VLPs preserved with thimerosal was shown to be dependent on the thimerosal concentration.Two highly neutralizing antibodies,13H12 and 3C3,were found to be the most sensitive to thimerosal treatment.The kinetics of antigenicity loss,when monitored with 13H12 or 3C3 as probes,yielded two distinctly different sets of kinetic parameters,while the data from both monoclonal antibodies(mAbs)followed a biphasic exponential decay model.The potential effect of thimerosal on protein function,particularly for thiolcontaining proteinaceous active components,needs to be comprehensively characterized during formulation development when a preservative is necessary.展开更多
The mass transfer of Rhodamine 6G from the droplet to the continuous phase in a coaxial micro-channel is studied using micro-LIF(Laser-Induced Fluorescence).The mass distribution inside droplet is measured and visuali...The mass transfer of Rhodamine 6G from the droplet to the continuous phase in a coaxial micro-channel is studied using micro-LIF(Laser-Induced Fluorescence).The mass distribution inside droplet is measured and visualized.The experimental results affirm that there exists the internal circulation inside the droplet and it could enhance the convective mass transfer.The stagnant center of vortices is also observed.The extraction fraction could reach 40%80%.In order to establish the mass transfer model,different flow rates of the dispersed and continuous phase are adopted.The high continuous phase flow rate and low dispersed phase flow rate are both beneficial to enhance mass transfer by expediting the internal circulation.A modified mass transfer model is found to calculate the extraction fraction.A good agreement between the model and experiment in various conditions demonstrates that the mass transfer model in this work is reliable and feasible.展开更多
High-fidelity two-qubit gates are essential for the realization of large-scale quantum computation and simulation.Tunable coupler design is used to reduce the problem of parasitic coupling and frequency crowding in ma...High-fidelity two-qubit gates are essential for the realization of large-scale quantum computation and simulation.Tunable coupler design is used to reduce the problem of parasitic coupling and frequency crowding in manyqubit systems and thus thought to be advantageous. Here we design an extensible 5-qubit system in which center transmon qubit can couple to every four near-neighboring qubits via a capacitive tunable coupler and experimentally demonstrate high-fidelity controlled-phase(CZ) gate by manipulating central qubit and one nearneighboring qubit. Speckle purity benchmarking and cross entropy benchmarking are used to assess the purity fidelity and the fidelity of the CZ gate. The average purity fidelity of the CZ gate is 99.69±0.04% and the average fidelity of the CZ gate is 99.65±0.04%, which means that the control error is about 0.04%. Our work is helpful for resolving many challenges in implementation of large-scale quantum systems.展开更多
The development of high-fidelity two-qubit quantum gates is essential for digital quantum computing.Here,we propose and realize an all-microwave parametric controlled-Z(CZ)gates by coupling strength modulation in a su...The development of high-fidelity two-qubit quantum gates is essential for digital quantum computing.Here,we propose and realize an all-microwave parametric controlled-Z(CZ)gates by coupling strength modulation in a superconducting Transmon qubit system with tunable couplers.After optimizing the design of the tunable coupler together with the control pulse numerically,we experimentally realized a 100 ns CZ gate with high fidelity of 99.38%±0.34%and the control error being 0.1%.We note that our CZ gates are not affected by pulse distortion and do not need pulse correction,providing a solution for the real-time pulse generation in a dynamic quantum feedback circuit.With the expectation of utilizing our all-microwave control scheme to reduce the number of control lines through frequency multiplexing in the future,our scheme draws a blueprint for the high-integrable quantum hardware design.展开更多
Cellulosic materials are attracting increasing research interest because of their abundance,biocompatibility,and biodegradability,making them suitable in multiple industrial and medical applications.Functionalization ...Cellulosic materials are attracting increasing research interest because of their abundance,biocompatibility,and biodegradability,making them suitable in multiple industrial and medical applications.Functionalization of cellulose is usually required to improve or expand its properties to meet the requirements of different applications.Cellulose-binding domains(CBDs)found in various proteins have been shown to be powerful tools in the functionalization of cellulose materials.In this review,we firstly introduce the structural characteristics of commonly used CBDs belonging to carbohydrate-binding module families 1,2 and 3.Then,we summarize four main kinds of methodologies for employing CBDs to modify cellulosic materials(i.e.,CBD only,genetic fusion,non-covalent linkage and covalent linkage).Via different approaches,CBDs have been used to improve the material properties of cellulose,immobilize enzymes for biocatalysis,and design various detection tools.To achieve industrial applications,researches for lowering the production cost of CBDs,improving their performance(e.g.,stability),and expanding their application scenarios are still in need.展开更多
For autonomous Unmanned Aerial Vehicles(UAVs)flying in real-world scenarios,time for path planning is always limited,which is a challenge known as the anytime problem.Anytime planners address this by finding a collisi...For autonomous Unmanned Aerial Vehicles(UAVs)flying in real-world scenarios,time for path planning is always limited,which is a challenge known as the anytime problem.Anytime planners address this by finding a collision-free path quickly and then improving it until time runs out,making UAVs more adaptable to different mission scenarios.However,current anytime algorithms based on A^(*)have insufficient control over the suboptimality bounds of paths and tend to lose their anytime properties in environments with large concave obstacles.This paper proposes a novel anytime path planning algorithm,Anytime Radiation A^(*)(ARa A^(*)),which can generate a series of suboptimal paths with improved bounds through decreasing search step sizes and can generate the optimal path when time is sufficient.The ARa A^(*)features two main innovations:an adaptive variable-step-size mechanism and elliptic constraints based on waypoints.The former helps achieve fast path searching in various environments.The latter allows ARa A^(*)to control the suboptimality bounds of paths and further enhance search efficiency.Simulation experiments show that the ARa A^(*)outperforms Anytime Repairing A^(*)(ARA^(*))and Anytime D^(*)(AD^(*))in controlling suboptimality bounds and planning time,especially in environments with large concave obstacles.Final flight experiments demonstrate that the paths planned by ARa A^(*)can ensure the safe flight of quadrotors.展开更多
An artificial neural network(ANN)method is introduced to predict drop size in two kinds of pulsed columns with small-scale data sets.After training,the deviation between calculate and experimental results are 3.8%and ...An artificial neural network(ANN)method is introduced to predict drop size in two kinds of pulsed columns with small-scale data sets.After training,the deviation between calculate and experimental results are 3.8%and 9.3%,respectively.Through ANN model,the influence of interfacial tension and pulsation intensity on the droplet diameter has been developed.Droplet size gradually increases with the increase of interfacial tension,and decreases with the increase of pulse intensity.It can be seen that the accuracy of ANN model in predicting droplet size outside the training set range is reach the same level as the accuracy of correlation obtained based on experiments within this range.For two kinds of columns,the drop size prediction deviations of ANN model are 9.6%and 18.5%and the deviations in correlations are 11%and 15%.展开更多
Fe–S compounds with hexagonal crystal structure are potential hydrogen permeation barrier during H2S corrosion. Hexagonal system Fe–S films were prepared on carbon steel through corrosion and CVD deposition, and the...Fe–S compounds with hexagonal crystal structure are potential hydrogen permeation barrier during H2S corrosion. Hexagonal system Fe–S films were prepared on carbon steel through corrosion and CVD deposition, and the barrier effect of different Fe–S films on hydrogen permeation was tested using electrochemical hydrogen permeation method. After that, the electrical properties of Fe–S compound during phase transformation were measured using thermoelectric measurement system. Results show that the mackinawite has no obvious barrier effect on hydrogen penetration, as a p-type semiconductor, and pyrrhotite (including troilite) has obvious barrier effect on hydrogen penetration,as an n-type semiconductor. Hydrogen permeation tests showed peak permeation performance when the surface was deposited with a continuous film of pyrrhotite (Fe_(1–x)S) and troilite. The FeS compounds suppressed hydrogen permeation by the promotion of the hydrogen evolution reaction, semiconducting inversion from p-to n-type, and the migration of ions at the interface.展开更多
In this study,a computational fluid dynamics(CFD)method was adopted to calculate axial dispersion coefficients of annular pulsed disc and doughnut columns(APDDCs).Passive tracer was uniformly injected by pulse input a...In this study,a computational fluid dynamics(CFD)method was adopted to calculate axial dispersion coefficients of annular pulsed disc and doughnut columns(APDDCs).Passive tracer was uniformly injected by pulse input at the continuous phase inlet,and its concentration governing equation was solved in liquid–liquidtwo-phase flow fields.The residence time distributions(RTDs)were obtained using the surface monitoring technique.The adopted RTD–CFD method was verified by comparing the axial dispersion coefficient between simulation and experimental results in the literature.However,in pilot-scale APDDCs,the axial dispersion coefficients predicted by the CFD–RTD method were approximately three times larger than experimental results determined by the steady-state concentration profile method.This experimental method was demonstrated to be insensitive to the variation of the axial dispersion coefficient.The CFD–RTD method was more recommended to determine the axial dispersion coefficient.It was found that the axial dispersion coefficient increased with an increase in pulsation intensity,column diameter,and plate spacing,but was little affected by the throughput.展开更多
Objectives To evaluate degrees and percentages of cyclorotation in Chinese patients during femtosecond laser assisted cataract surgery performed by using LenSx femtosecond laser platform and to further sub-analyze bil...Objectives To evaluate degrees and percentages of cyclorotation in Chinese patients during femtosecond laser assisted cataract surgery performed by using LenSx femtosecond laser platform and to further sub-analyze bilateral cases to assess the congruency between both eyes. Methods This was a single-centre retrospective study(Beijing AIER-Intec Eye Hospital, Beijing, China) in which the records of 330 eyes of 274 patients who underwent femtosecond laser-assisted cataract surgery in 1 or two eyes(with placement of posterior chamber IOL) between May 2017 and July 2019 by using femtosecond laser platform(LenSx) were reviewed.LenSx Femtosecond Laser(Alcon Laboratories, Inc, Fort Worth, Texas, USA) was used to carry out corneal incisions, anterior capsulotomies and lens fragmentation in this procedure. All the patients were marked on corneal limbus(at 3 and 9 o’clock positions) by using sterile skin marker(Medplus.inc) while sitting upright preoperatively. Percentages of cyclorotation were calculated. Absolutes values were taken for all calculations. The cyclorotation data was tested to determine whether it followed a normal distribution. Descriptive statistics were applied to calculate percentile values of cyclorotation which is presented as(M [P25;P75]). Results This retrospective study evaluated 330 eyes of 274 Chinese patients who had femtosecond laser-assisted cataract surgery using the femtosecond laser platform. Of the 330 eyes, there were 90 eyes(27.27%) that did not show cyclorotation. Out of 330 eyes that were analyzed, 72.72%(240 eyes) exhibited cyclorotation. The median value of cyclorotation was 3.0[0.0;5.0](ranged between 0 to 19 degrees). Of the 330 eyes, there were 90 eyes(27.27%) that did not show cyclorotation. Overall anticlockwise rotation(172 eyes, 52.12%) was more common than clockwise rotation(68 eyes, 20.60%).The total numbers of right and left eyes were 169 and 161 respectively. In terms of laterality, 43.78% of right eyes(74 eyes out of 169) and 60.86% of left eyes(98 eyes out of 161) showed anticlockwise rotation. 25.44% of right eyes(43 eyes) and 15.52% of left eyes(25 eyes) exhibited clockwise rotation. Therefore anticlockwise rotation was considered as common occurrence in both left and right eyes. In order to assess the congruency of cyclorotation between eyes in bilateral cases, a subanalysis of all the patients who underwent bilateral femtosecond laser-assisted cataract surgery(56 out of 274) was performed. In total, 17 patients exhibited incylorotation(both eyes moved nasally) and 4 patients showed motion of eyes temporally exhibiting excyclorotation. In rest of patients some of them showed no rotation and others exhibited the movement of both eyes in opposite direction(one eye nasally and other temporally and vice versa).There were no AK-related complications or complications during femtosecond laser-assisted cataract surgery. Among them 56 were bilateral cases. Conclusion Clinically significant cyclorotation can occur during femtosecond laser assisted cataract surgery causing axis misalignment which might influence the desired astigmatism correction outcomes. It is highly recommended to take crucial compensatory measures to address this problem.展开更多
Epstein-Barr virus(EBV),the first identified human tumor virus,is implicated in various human malignancies,infectious mononucleosis,and more recently,multiple sclerosis.Prophylactic vaccines have the potential to effe...Epstein-Barr virus(EBV),the first identified human tumor virus,is implicated in various human malignancies,infectious mononucleosis,and more recently,multiple sclerosis.Prophylactic vaccines have the potential to effectively prevent EBV infection.Glycoprotein B(gB)serves as the fusogen and plays a pivotal role in the virus entry process,making it a critical target for EBV vaccine development.Surface membrane proteins of enveloped viruses serve as native conformational antigens,making them susceptible to immune recognition.Utilizing lipid membrane-bound viral antigens is a promising strategy for effective vaccine presentation in this context.In this study,we employed a truncated design for gB proteins,observing that these truncated gB proteins prompted a substantial release of extracellular vesicles(EVs)in insect cells.We verified that EVs exhibited abundant gB proteins,displaying the typical virus particle morphology and extracellular vesicle characteristics.gB EVs demonstrated a more efficient humoral and cellular immune response compared with the gB ectodomain trimer vaccine in mice.Moreover,the antisera induced by the gB EVs vaccine exhibited robust antibody-dependent cytotoxicity.Consequently,gB EVs-based vaccines hold significant potential for preventing EBV infection and offer valuable insights for vaccine design.展开更多
To ensure a long-term quantum computational advantage,the quantum hardware should be upgraded to withstand the competition of continuously improved classical algorithms and hardwares.Here,we demonstrate a superconduct...To ensure a long-term quantum computational advantage,the quantum hardware should be upgraded to withstand the competition of continuously improved classical algorithms and hardwares.Here,we demonstrate a superconducting quantum computing systems Zuchongzhi 2.1,which has 66 qubits in a two-dimensional array in a tunable coupler architecture.The readout fidelity of Zuchongzhi 2.1 is considerably improved to an average of 97.74%.The more powerful quantum processor enables us to achieve larger-scale random quantum circuit sampling,with a system scale of up to 60 qubits and 24 cycles,and fidelity of FXEB=(3·66±0·345)×10^(-4).The achieved sampling task is about 6 orders of magnitude more difficult than that of Sycamore[Nature 574,505(2019)]in the classic simulation,and 3 orders of magnitude more difficult than the sampling task on Zuchongzhi 2.0[arXiv:2106.14734(2021)].The time consumption of classically simulating random circuit sampling experiment using state-of-the-art classical algorithm and supercomputer is extended to tens of thousands of years(about 4·8×104years),while Zuchongzhi 2.1 only takes about 4.2 h,thereby significantly enhancing the quantum computational advantage.展开更多
Varicella-zoster virus(VZV)is a highly infectious agent responsible for both varicella and herpes zoster disease.Despite high efficacy,there remain safety and accessibility concerns with the licensed vaccines.Here,we ...Varicella-zoster virus(VZV)is a highly infectious agent responsible for both varicella and herpes zoster disease.Despite high efficacy,there remain safety and accessibility concerns with the licensed vaccines.Here,we sought to produce a VZV g E immunogen using an E.coli expression system.We found that the soluble expression and yield of g E protein could be enhanced via C-terminal truncations to the protein,thereby facilitating a robust and scalable purification process for the purpose of vaccine manufacturing.The lead truncated g E(aa 31–358),hereafter referred to as tg E,was a homogenous monomer in solution and showed excellent antigenicity.Finally,we assessed and compared the immunogenicity of tg E with commercial v Oka LAV and Shingrix vaccine.We found that aluminum-adjuvanted tg E was immunogenic as compared with v Oka LAV.When adjuvanted with AS01B,a two-dose immunization of tg E showed comparable or better potency in antibody responses and cell-mediated immunity with those of the Shingrix vaccine at the same dosage,especially in terms of the proportion of IFN-γ-expressing CD4^(+)T cells.In conclusion,this method of E.coli-mediate tg E expression offers a cost-effective and scalable strategy to generate an ideal VZV g E immunogen for the development of both varicella and zoster vaccines.展开更多
Continual evolution of the severe acute respiratory syndrome coronavirus(SARS-CoV-2)virus has allowed for its gradual evasion of neutralizing antibodies(nAbs)produced in response to natural infection or vaccination.Th...Continual evolution of the severe acute respiratory syndrome coronavirus(SARS-CoV-2)virus has allowed for its gradual evasion of neutralizing antibodies(nAbs)produced in response to natural infection or vaccination.The rapid nature of these changes has incited a need for the development of superior broad nAbs(bnAbs)and/or the rational design of an antibody cocktail that can protect against the mutated virus strain.Here,we report two angiotensin-converting enzyme 2 competing nAbs—8H12 and 3E2—with synergistic neutralization but evaded by some Omicron subvariants.Cryo-electron microscopy reveals the two nAbs synergistic neutralizing virus through a rigorous pairing permitted by rearrangement of the 472-489 loop in the receptor-binding domain to avoid steric clashing.Bispecific antibodies based on these two nAbs tremendously extend the neutralizing breadth and restore neutralization against recent variants including currently dominant XBB.1.5.Together,these findings expand our understanding of the potential strategies for the neutralization of SARS-CoV-2 variants toward the design of broad-acting antibody therapeutics and vaccines.展开更多
Dear Editor After 14-years of development, the first prophylactic vaccine against the Hepatitis E virus （HEY） has been marketed since 2012 （Wu et al., 2012）o However, the neutralizing epitopes of HEY are not compl...Dear Editor After 14-years of development, the first prophylactic vaccine against the Hepatitis E virus （HEY） has been marketed since 2012 （Wu et al., 2012）o However, the neutralizing epitopes of HEY are not completely defined. E2s, a protruding homodi- mer domain of HEV capsid protein, is responsible for inter- acting with host cells to initiate infection （Li et al., 2009; Li et al., 2005）. It was shown that two monoclonal antibodies （mAb）, 8Cll and 8H3, could neutralize the infectivity of HEV in rhesus, and the two mAbs bind to different neutralizing conformational epitopes on E2s （Zhang et al., 2005）.展开更多
基金This work was funded by the National Key R&D Program of China(2019YFC1509205)the National Natural Science Foundation of China(Nos.42174023 and 41804015)+1 种基金the Postgraduate Scientific Research Innovation Project of Hunan Province(150110074)the Postgraduate Scientific Research Innovation Project of Central South University(212191010).
文摘In the past 30 years,the small baseline subset(SBAS)InSAR time-series technique has emerged as an essential tool for measuring slow surface displacement and estimating geophysical parameters.Because of its ability to monitor large-scale deformation with millimeter accuracy,the SBAS method has been widely used in various geodetic fields,such as ground subsidence,landslides,and seismic activity.The obtained long-term time-series cumulative deformation is vital for studying the deformation mecha-nism.This article reviews the algorithms,applications,and challenges of the SBAS method.First,we recall the fundamental principle and analyze the shortcomings of the traditional SBAS algorithm,which provides a basic framework for the following improved time series methods.Second,we classify the current improved SBAS techniques from different perspectives:solving the ill-posed equation,increasing the density of high-coherence points,improving the accuracy of monitoring deformation and measuring the multi-dimensional deformation.Third,we summarize the application of the SBAS method in monitoring ground subsidence,permafrost degradation,glacier movement,volcanic activity,landslides,and seismic activity.Finally,we discuss the difficulties faced by the SBAS method and explore its future development direction.
基金the supports of the National Natural Science Foundation of China(2147612121322604)for this work。
文摘The well understanding of interaction forces between single dispersed droplets is crucial to the understanding of emulsion stabilization mechanism.Recently,many studies have reported the direct quantitative measurements of interaction forces between 20-200μm single droplet coated polymers by atomic force microscope(AFM).These studies have revealed many important results about the relationship of the interaction forces and the droplet deformation.However,these studies of the quantitative relationship between the measured interaction forces and the separation distance of the front end of the droplet have rarely been reported.Optical tweezer instrument can make it possible to establish the quantitative relationship between the measured force and the separation distance of the front end of the droplet,which will make better understanding of the interaction mechanisms between droplets.Due to the differences of the measuring mechanism between atomic force microscopy(AFM)and optical tweezers,the theory model of AFM measurements cannot be fitted with the force measurement by optical tweezers.We have made an exhaustive comparison of the measuring differences between AFM and optical tweezer instrument in this work.Moreover,we built a numerical model to derive the repulsive pressure through the measured force curve in order to quantify the measured force of two micron-sized oil droplet coated polymers by optical tweezers.Furthermore,the novel method can be extended to other micron-sized emulsion systems,and these findings will be a vital progress on quantitative force measurements between micron-sized droplets.
基金financially supported by research grants from the Natural Science Foundation of China [Grant No. 62074022 (K.S.), 12004057 (Y.J.Z.), 52173235 (M.L.)]the Natural Science Foundation of Chongqing [cstc2021jcyj-jqX0015 (K.S.)]+3 种基金Chongqing Talent Plan [cstc2021ycjh-bgzxm0334 (S.S.C.), CQYC2021059206 (K.S.)]Fundamental Research Funds for the Central Universities [No. 2020CDJQY-A055 (K.S.)]the Key Laboratory of Low-grade Energy Utilization Technologies and Systems [Grant No. LLEUTS-201901 (K.S.)]support from Chongqing Postgraduate Research and Innovation Project (CYS22032)。
文摘Ionic thermoelectrics(i-TE) possesses great potential in powering distributed electronics because it can generate thermopower up to tens of millivolts per Kelvin. However,as ions cannot enter external circuit, the utilization of i-TE is currently based on capacitive charge/discharge, which results in discontinuous working mode and low energy density. Here,we introduce an ion–electron thermoelectric synergistic(IETS)effect by utilizing an ion–electron conductor. Electrons/holes can drift under the electric field generated by thermodiffusion of ions, thus converting the ionic current into electrical current that can pass through the external circuit. Due to the IETS effect, i-TE is able to operate continuously for over 3000 min.Moreover, our i-TE exhibits a thermopower of 32.7 mV K^(-1) and an energy density of 553.9 J m^(-2), which is more than 6.9 times of the highest reported value. Consequently, direct powering of electronics is achieved with i-TE. This work provides a novel strategy for the design of high-performance i-TE materials.
基金Thisworkwas funded by the National Natural Science Foundation of China(Grant Nos.:81993149041,and U1705283)the National Science and Technology Major Project,China(Project No.:2018ZX09303005-002)+1 种基金Fujian Health Education Joint Research Project,China(Project No.:2019-WJ-05)Xiamen Science and Technology Major Project,China(Project No.:3502Z20193009).
文摘Thimerosal has been widely used as a preservative in drug and vaccine products for decades.Due to the strong propensity to modify thiols in proteins,conformational changes could occur due to covalent bond formation between ethylmercury(a degradant of thimerosal)and thiols.Such a conformational change could lead to partial or even complete loss of desirable protein function.This study aims to investigate the effects of thimerosal on the capsid stability and antigenicity of recombinant human papillomavirus(HPV)18 virus-like particles(VLPs).Dramatic destabilization of the recombinant viral capsid upon thimerosal treatment was observed.Such a negative effect on the thermal stability of VLPs preserved with thimerosal was shown to be dependent on the thimerosal concentration.Two highly neutralizing antibodies,13H12 and 3C3,were found to be the most sensitive to thimerosal treatment.The kinetics of antigenicity loss,when monitored with 13H12 or 3C3 as probes,yielded two distinctly different sets of kinetic parameters,while the data from both monoclonal antibodies(mAbs)followed a biphasic exponential decay model.The potential effect of thimerosal on protein function,particularly for thiolcontaining proteinaceous active components,needs to be comprehensively characterized during formulation development when a preservative is necessary.
基金the National Natural Science Foundation of China(21991100,21991101)for this work.
文摘The mass transfer of Rhodamine 6G from the droplet to the continuous phase in a coaxial micro-channel is studied using micro-LIF(Laser-Induced Fluorescence).The mass distribution inside droplet is measured and visualized.The experimental results affirm that there exists the internal circulation inside the droplet and it could enhance the convective mass transfer.The stagnant center of vortices is also observed.The extraction fraction could reach 40%80%.In order to establish the mass transfer model,different flow rates of the dispersed and continuous phase are adopted.The high continuous phase flow rate and low dispersed phase flow rate are both beneficial to enhance mass transfer by expediting the internal circulation.A modified mass transfer model is found to calculate the extraction fraction.A good agreement between the model and experiment in various conditions demonstrates that the mass transfer model in this work is reliable and feasible.
基金the National Key R&D Program of China(Grant No.2017YFA0304300)the Chinese Academy of Sciences+6 种基金Anhui Initiative in Quantum Information TechnologiesTechnology Committee of Shanghai Municipalitythe National Natural Science Foundation of China(Grants Nos.11905217,11774326,and 11905294)the Shanghai Municipal Science and Technology Major Project(Grant No.2019SHZDZX01)the Natural Science Foundation of Shanghai(Grant No.19ZR1462700)the Key-Area Research and Development Program of Guangdong Provice(Grant No.2020B0303030001)the Youth Talent Lifting Project(Grant No.2020-JCJQ-QT-030)。
文摘High-fidelity two-qubit gates are essential for the realization of large-scale quantum computation and simulation.Tunable coupler design is used to reduce the problem of parasitic coupling and frequency crowding in manyqubit systems and thus thought to be advantageous. Here we design an extensible 5-qubit system in which center transmon qubit can couple to every four near-neighboring qubits via a capacitive tunable coupler and experimentally demonstrate high-fidelity controlled-phase(CZ) gate by manipulating central qubit and one nearneighboring qubit. Speckle purity benchmarking and cross entropy benchmarking are used to assess the purity fidelity and the fidelity of the CZ gate. The average purity fidelity of the CZ gate is 99.69±0.04% and the average fidelity of the CZ gate is 99.65±0.04%, which means that the control error is about 0.04%. Our work is helpful for resolving many challenges in implementation of large-scale quantum systems.
基金the USTC Center for Micro-and Nanoscale Research and Fabrication for supporting the sample fabricationQuantum CTek Co.,Ltd.for supporting the fabrication and the maintenance of room-temperature electronics+7 种基金supported by the National Key R&D Program of China(Grant No.2017YFA0304300)the Chinese Academy of Sciencesthe Anhui Initiative in Quantum Information Technologiesthe Technology Committee of Shanghai Municipalitythe National Natural Science Foundation of China(Grants No.11905217 and 11905294)the Natural Science Foundation of Shanghai(Grant No.19ZR1462700)he Key-Area Research and Development Program of Guangdong Province(Grant No.2020B0303030001)the China Postdoctoral Science Foundation。
文摘The development of high-fidelity two-qubit quantum gates is essential for digital quantum computing.Here,we propose and realize an all-microwave parametric controlled-Z(CZ)gates by coupling strength modulation in a superconducting Transmon qubit system with tunable couplers.After optimizing the design of the tunable coupler together with the control pulse numerically,we experimentally realized a 100 ns CZ gate with high fidelity of 99.38%±0.34%and the control error being 0.1%.We note that our CZ gates are not affected by pulse distortion and do not need pulse correction,providing a solution for the real-time pulse generation in a dynamic quantum feedback circuit.With the expectation of utilizing our all-microwave control scheme to reduce the number of control lines through frequency multiplexing in the future,our scheme draws a blueprint for the high-integrable quantum hardware design.
基金supported by the National Natural Science Foundation of China(32170037)and SKLMT Frontiers and Challenges Project(SKLMTFCP-2023-04).
文摘Cellulosic materials are attracting increasing research interest because of their abundance,biocompatibility,and biodegradability,making them suitable in multiple industrial and medical applications.Functionalization of cellulose is usually required to improve or expand its properties to meet the requirements of different applications.Cellulose-binding domains(CBDs)found in various proteins have been shown to be powerful tools in the functionalization of cellulose materials.In this review,we firstly introduce the structural characteristics of commonly used CBDs belonging to carbohydrate-binding module families 1,2 and 3.Then,we summarize four main kinds of methodologies for employing CBDs to modify cellulosic materials(i.e.,CBD only,genetic fusion,non-covalent linkage and covalent linkage).Via different approaches,CBDs have been used to improve the material properties of cellulose,immobilize enzymes for biocatalysis,and design various detection tools.To achieve industrial applications,researches for lowering the production cost of CBDs,improving their performance(e.g.,stability),and expanding their application scenarios are still in need.
基金the support of the National Natural Science Foundation of China(No.52272382)the Aeronautical Science Foundation of China(No.20200017051001)the Fundamental Research Funds for the Central Universities,China。
文摘For autonomous Unmanned Aerial Vehicles(UAVs)flying in real-world scenarios,time for path planning is always limited,which is a challenge known as the anytime problem.Anytime planners address this by finding a collision-free path quickly and then improving it until time runs out,making UAVs more adaptable to different mission scenarios.However,current anytime algorithms based on A^(*)have insufficient control over the suboptimality bounds of paths and tend to lose their anytime properties in environments with large concave obstacles.This paper proposes a novel anytime path planning algorithm,Anytime Radiation A^(*)(ARa A^(*)),which can generate a series of suboptimal paths with improved bounds through decreasing search step sizes and can generate the optimal path when time is sufficient.The ARa A^(*)features two main innovations:an adaptive variable-step-size mechanism and elliptic constraints based on waypoints.The former helps achieve fast path searching in various environments.The latter allows ARa A^(*)to control the suboptimality bounds of paths and further enhance search efficiency.Simulation experiments show that the ARa A^(*)outperforms Anytime Repairing A^(*)(ARA^(*))and Anytime D^(*)(AD^(*))in controlling suboptimality bounds and planning time,especially in environments with large concave obstacles.Final flight experiments demonstrate that the paths planned by ARa A^(*)can ensure the safe flight of quadrotors.
基金the support of the National Natural Science Foundation of China(22278234,21776151)。
文摘An artificial neural network(ANN)method is introduced to predict drop size in two kinds of pulsed columns with small-scale data sets.After training,the deviation between calculate and experimental results are 3.8%and 9.3%,respectively.Through ANN model,the influence of interfacial tension and pulsation intensity on the droplet diameter has been developed.Droplet size gradually increases with the increase of interfacial tension,and decreases with the increase of pulse intensity.It can be seen that the accuracy of ANN model in predicting droplet size outside the training set range is reach the same level as the accuracy of correlation obtained based on experiments within this range.For two kinds of columns,the drop size prediction deviations of ANN model are 9.6%and 18.5%and the deviations in correlations are 11%and 15%.
基金financailly supported by the National Natural Science Foundation of China (Nos.52275198 and 51805292)the Beijing Natural Science Foundation,China(No.2202020)the Tribology Science Fund of State Key Laboratory of Tribology,China (No.SKLT2022B11)。
文摘Fe–S compounds with hexagonal crystal structure are potential hydrogen permeation barrier during H2S corrosion. Hexagonal system Fe–S films were prepared on carbon steel through corrosion and CVD deposition, and the barrier effect of different Fe–S films on hydrogen permeation was tested using electrochemical hydrogen permeation method. After that, the electrical properties of Fe–S compound during phase transformation were measured using thermoelectric measurement system. Results show that the mackinawite has no obvious barrier effect on hydrogen penetration, as a p-type semiconductor, and pyrrhotite (including troilite) has obvious barrier effect on hydrogen penetration,as an n-type semiconductor. Hydrogen permeation tests showed peak permeation performance when the surface was deposited with a continuous film of pyrrhotite (Fe_(1–x)S) and troilite. The FeS compounds suppressed hydrogen permeation by the promotion of the hydrogen evolution reaction, semiconducting inversion from p-to n-type, and the migration of ions at the interface.
基金support of the National Natural Science Foundation of China(21776151 and 21576147)for this investigation。
文摘In this study,a computational fluid dynamics(CFD)method was adopted to calculate axial dispersion coefficients of annular pulsed disc and doughnut columns(APDDCs).Passive tracer was uniformly injected by pulse input at the continuous phase inlet,and its concentration governing equation was solved in liquid–liquidtwo-phase flow fields.The residence time distributions(RTDs)were obtained using the surface monitoring technique.The adopted RTD–CFD method was verified by comparing the axial dispersion coefficient between simulation and experimental results in the literature.However,in pilot-scale APDDCs,the axial dispersion coefficients predicted by the CFD–RTD method were approximately three times larger than experimental results determined by the steady-state concentration profile method.This experimental method was demonstrated to be insensitive to the variation of the axial dispersion coefficient.The CFD–RTD method was more recommended to determine the axial dispersion coefficient.It was found that the axial dispersion coefficient increased with an increase in pulsation intensity,column diameter,and plate spacing,but was little affected by the throughput.
基金supported by the Aier Eye Hospital Group Scientific Research Project Grant(No.AF152D01).
文摘Objectives To evaluate degrees and percentages of cyclorotation in Chinese patients during femtosecond laser assisted cataract surgery performed by using LenSx femtosecond laser platform and to further sub-analyze bilateral cases to assess the congruency between both eyes. Methods This was a single-centre retrospective study(Beijing AIER-Intec Eye Hospital, Beijing, China) in which the records of 330 eyes of 274 patients who underwent femtosecond laser-assisted cataract surgery in 1 or two eyes(with placement of posterior chamber IOL) between May 2017 and July 2019 by using femtosecond laser platform(LenSx) were reviewed.LenSx Femtosecond Laser(Alcon Laboratories, Inc, Fort Worth, Texas, USA) was used to carry out corneal incisions, anterior capsulotomies and lens fragmentation in this procedure. All the patients were marked on corneal limbus(at 3 and 9 o’clock positions) by using sterile skin marker(Medplus.inc) while sitting upright preoperatively. Percentages of cyclorotation were calculated. Absolutes values were taken for all calculations. The cyclorotation data was tested to determine whether it followed a normal distribution. Descriptive statistics were applied to calculate percentile values of cyclorotation which is presented as(M [P25;P75]). Results This retrospective study evaluated 330 eyes of 274 Chinese patients who had femtosecond laser-assisted cataract surgery using the femtosecond laser platform. Of the 330 eyes, there were 90 eyes(27.27%) that did not show cyclorotation. Out of 330 eyes that were analyzed, 72.72%(240 eyes) exhibited cyclorotation. The median value of cyclorotation was 3.0[0.0;5.0](ranged between 0 to 19 degrees). Of the 330 eyes, there were 90 eyes(27.27%) that did not show cyclorotation. Overall anticlockwise rotation(172 eyes, 52.12%) was more common than clockwise rotation(68 eyes, 20.60%).The total numbers of right and left eyes were 169 and 161 respectively. In terms of laterality, 43.78% of right eyes(74 eyes out of 169) and 60.86% of left eyes(98 eyes out of 161) showed anticlockwise rotation. 25.44% of right eyes(43 eyes) and 15.52% of left eyes(25 eyes) exhibited clockwise rotation. Therefore anticlockwise rotation was considered as common occurrence in both left and right eyes. In order to assess the congruency of cyclorotation between eyes in bilateral cases, a subanalysis of all the patients who underwent bilateral femtosecond laser-assisted cataract surgery(56 out of 274) was performed. In total, 17 patients exhibited incylorotation(both eyes moved nasally) and 4 patients showed motion of eyes temporally exhibiting excyclorotation. In rest of patients some of them showed no rotation and others exhibited the movement of both eyes in opposite direction(one eye nasally and other temporally and vice versa).There were no AK-related complications or complications during femtosecond laser-assisted cataract surgery. Among them 56 were bilateral cases. Conclusion Clinically significant cyclorotation can occur during femtosecond laser assisted cataract surgery causing axis misalignment which might influence the desired astigmatism correction outcomes. It is highly recommended to take crucial compensatory measures to address this problem.
基金supported by the National Natural Science Foundation of China(82073756).
文摘Epstein-Barr virus(EBV),the first identified human tumor virus,is implicated in various human malignancies,infectious mononucleosis,and more recently,multiple sclerosis.Prophylactic vaccines have the potential to effectively prevent EBV infection.Glycoprotein B(gB)serves as the fusogen and plays a pivotal role in the virus entry process,making it a critical target for EBV vaccine development.Surface membrane proteins of enveloped viruses serve as native conformational antigens,making them susceptible to immune recognition.Utilizing lipid membrane-bound viral antigens is a promising strategy for effective vaccine presentation in this context.In this study,we employed a truncated design for gB proteins,observing that these truncated gB proteins prompted a substantial release of extracellular vesicles(EVs)in insect cells.We verified that EVs exhibited abundant gB proteins,displaying the typical virus particle morphology and extracellular vesicle characteristics.gB EVs demonstrated a more efficient humoral and cellular immune response compared with the gB ectodomain trimer vaccine in mice.Moreover,the antisera induced by the gB EVs vaccine exhibited robust antibody-dependent cytotoxicity.Consequently,gB EVs-based vaccines hold significant potential for preventing EBV infection and offer valuable insights for vaccine design.
基金the National Key R&D Program of China(2017YFA0304300),the Chinese Academy of Sciences,Anhui Initiative in Quantum Information Technologies,Technology Committee of Shanghai Municipality,National Natural Science Foundation of China(11905217,11774326,and 11905294)‘Shang-hai Municipal Science and Technology Major Project(2019SHZDZX01)’Natural Science Foundation of Shanghai(19ZR1462700)‘Key-Area Research and Development Program of Guangdong Province(2020B0303030001)’the Youth Talent Lifting Project(2020-JCJQ-QT-030)。
文摘To ensure a long-term quantum computational advantage,the quantum hardware should be upgraded to withstand the competition of continuously improved classical algorithms and hardwares.Here,we demonstrate a superconducting quantum computing systems Zuchongzhi 2.1,which has 66 qubits in a two-dimensional array in a tunable coupler architecture.The readout fidelity of Zuchongzhi 2.1 is considerably improved to an average of 97.74%.The more powerful quantum processor enables us to achieve larger-scale random quantum circuit sampling,with a system scale of up to 60 qubits and 24 cycles,and fidelity of FXEB=(3·66±0·345)×10^(-4).The achieved sampling task is about 6 orders of magnitude more difficult than that of Sycamore[Nature 574,505(2019)]in the classic simulation,and 3 orders of magnitude more difficult than the sampling task on Zuchongzhi 2.0[arXiv:2106.14734(2021)].The time consumption of classically simulating random circuit sampling experiment using state-of-the-art classical algorithm and supercomputer is extended to tens of thousands of years(about 4·8×104years),while Zuchongzhi 2.1 only takes about 4.2 h,thereby significantly enhancing the quantum computational advantage.
基金supported by the National Key Research and Development Program of China(2021YFC2301404)the National Natural Science Foundation of China(81991490)+2 种基金the Industry-University-Academy Cooperation Program of Xiamen(2022CXY0107)the Principal Fund(20720220006 and 20720220004)CAMS Innovation Fund for Medical Sciences(2019RU022)。
文摘Varicella-zoster virus(VZV)is a highly infectious agent responsible for both varicella and herpes zoster disease.Despite high efficacy,there remain safety and accessibility concerns with the licensed vaccines.Here,we sought to produce a VZV g E immunogen using an E.coli expression system.We found that the soluble expression and yield of g E protein could be enhanced via C-terminal truncations to the protein,thereby facilitating a robust and scalable purification process for the purpose of vaccine manufacturing.The lead truncated g E(aa 31–358),hereafter referred to as tg E,was a homogenous monomer in solution and showed excellent antigenicity.Finally,we assessed and compared the immunogenicity of tg E with commercial v Oka LAV and Shingrix vaccine.We found that aluminum-adjuvanted tg E was immunogenic as compared with v Oka LAV.When adjuvanted with AS01B,a two-dose immunization of tg E showed comparable or better potency in antibody responses and cell-mediated immunity with those of the Shingrix vaccine at the same dosage,especially in terms of the proportion of IFN-γ-expressing CD4^(+)T cells.In conclusion,this method of E.coli-mediate tg E expression offers a cost-effective and scalable strategy to generate an ideal VZV g E immunogen for the development of both varicella and zoster vaccines.
基金supported by grants from the National Key Research and Development Program of China (No.2021YFC2301404)the National Natural Science Foundation of China (Nos.81991491,82272305,and 32170942)+4 种基金Fujian Natural Science Foundation (No.2020J06007)the Industry-University-Research Project of Xiamen (No.2022CXY0106)the Fundamental Research Funds for the Central Universities (Nos.20720220006 and 20720220004)National Postdoctoral Science Foundation of China (No.2021M712659)CAMS Innovation Fund for Medical Sciences (No.2019RU022).
文摘Continual evolution of the severe acute respiratory syndrome coronavirus(SARS-CoV-2)virus has allowed for its gradual evasion of neutralizing antibodies(nAbs)produced in response to natural infection or vaccination.The rapid nature of these changes has incited a need for the development of superior broad nAbs(bnAbs)and/or the rational design of an antibody cocktail that can protect against the mutated virus strain.Here,we report two angiotensin-converting enzyme 2 competing nAbs—8H12 and 3E2—with synergistic neutralization but evaded by some Omicron subvariants.Cryo-electron microscopy reveals the two nAbs synergistic neutralizing virus through a rigorous pairing permitted by rearrangement of the 472-489 loop in the receptor-binding domain to avoid steric clashing.Bispecific antibodies based on these two nAbs tremendously extend the neutralizing breadth and restore neutralization against recent variants including currently dominant XBB.1.5.Together,these findings expand our understanding of the potential strategies for the neutralization of SARS-CoV-2 variants toward the design of broad-acting antibody therapeutics and vaccines.
文摘Dear Editor After 14-years of development, the first prophylactic vaccine against the Hepatitis E virus （HEY） has been marketed since 2012 （Wu et al., 2012）o However, the neutralizing epitopes of HEY are not completely defined. E2s, a protruding homodi- mer domain of HEV capsid protein, is responsible for inter- acting with host cells to initiate infection （Li et al., 2009; Li et al., 2005）. It was shown that two monoclonal antibodies （mAb）, 8Cll and 8H3, could neutralize the infectivity of HEV in rhesus, and the two mAbs bind to different neutralizing conformational epitopes on E2s （Zhang et al., 2005）.
