Given the importance of the intestinal microbiota in life-long health,increasing attention has been paid to ecological mechanisms that govern microbial succession.Both environmental dispersal and host priority effect ...Given the importance of the intestinal microbiota in life-long health,increasing attention has been paid to ecological mechanisms that govern microbial succession.Both environmental dispersal and host priority effect play important roles in intestinal microbiota succession of aquatic animals,but their relative importance is unknown.Here,we explore the intestinal microbiota succession and assembly of Litopenaeus vannamei across larvae,postlarvae,juvenile,and preadult stages.We aimed to clarify the relative contributions between environmental dispersal and host priority effect on intestinal microbiota succession.We found that bothα-andβ-diversity of the intestinal microbiota changed dynamically along with host development.In particular,the intestinal microbiota assemblies were dominated by stochastic processes,except with the larvae stage.The succession of shrimp intestinal microbiota was clearly influenced by internal communities in the intestines of larvae,as well as the external communities in water and sediment.Importantly,the impact of environmental dispersal on the intestinal microbiota succession outweighed the host priority effect during the larvae and postlarvae stages.However,this situation was largely reversed during the juvenile and preadult stages.The possible reason is that,during the larvae and postlarvae stages,shrimp mainly feed on plankton from the environment,and their digestive system remains underdeveloped,the host recruits numerous microbes from the environment and selects specific microbes to aid digestion and nutrient absorption.These findings enhance our understanding of alternate effects of environmental and host factors on the intestinal microbiota succession of aquatic animals and provide a foundation for developing microecological management strategies in shrimp culture.展开更多
Antibiotic resistance genes(ARGs), human pathogenic bacteria(HPB), and HPB carrying ARGs are public issues that pose a high risk to aquatic environments and public health. Their diversity and abundance in water, intes...Antibiotic resistance genes(ARGs), human pathogenic bacteria(HPB), and HPB carrying ARGs are public issues that pose a high risk to aquatic environments and public health. Their diversity and abundance in water, intestine, and sediments of shrimp culture pond were investigated using metagenomic approach. A total of 19 classes of ARGs, 52 HPB species, and 7 species of HPB carrying ARGs were found. Additionally, 157, 104, and 86 subtypes of ARGs were detected in shrimp intestine, pond water, and sediment samples, respectively. In all the samples, multidrug resistance genes were the highest abundant class of ARGs. The dominant HPB was Enterococcus faecalis in shrimp intestine, Vibrio parahaemolyticus in sediments, and Mycobacterium yongonense in water, respectively. Moreover, E. faecalis(contig Intestine_364647)and Enterococcus faecium(contig Intestine_80272) carrying efr A, efr B and ANT(6)-Ia were found in shrimp intestine, Desulfosaricina cetonica(contig Sediment_825143) and Escherichia coli(contig Sediment_188430) carrying mex B and APH(3′)-IIa were found in sediments, and Laribacter hongkongensis(contig Water_478168 and Water_369477), Shigella sonnei(contig Water_880246),and Acinetobacter baumannii(contig Water_525520) carrying sul1, sul2, ere A, qac H, OXA-21, and mph D were found in pond water. Mobile genetic elements(MGEs) analysis indicated that horizontal gene transfer(HGT) of integrons, insertion sequences, and plasmids existed in shrimp intestine, sediment, and water samples, and the abundance of integrons was higher than that of other two MGEs. The results suggested that HPB carrying ARGs potentially existed in aquatic environments, and that these contributed to the environment and public health risk evaluation.展开更多
Sedimentary bacterial and eukaryotic communities are major components of the aquatic ecosystem.Revealing the linkages between their community structure and interactions is crucial to understand the diversity and funct...Sedimentary bacterial and eukaryotic communities are major components of the aquatic ecosystem.Revealing the linkages between their community structure and interactions is crucial to understand the diversity and functions of aquatic and soil ecosystems.However,how their diversity and assembly contribute to their interactions on time scale is unclear.This study examined sedimentary bacterial and eukaryotic communities in shrimp culture ponds at different culture stages.The most abundant bacteria were Proteobacteria(38.27%),whereas the most abundant eukaryotes were Chytridiomycota(27.48%).Bacterial and eukaryotic diversities were correlated(P<0.05),implying the strong interactions between bacteria and eukaryotes.Results showed that the bacterial and eukaryotic communities became increasingly similar on a local scale along with the shrimp culture.Only the eukaryotic community significantly increased in similarity along with the shrimp culture(P<0.05),suggesting that the sedimentary eukaryotic community structure is sensitive under shrimp culture.Co-occurrence network modeling indicated that positive microbial interactions were dominant.The homogeneous selection was the major driver of community assembly.Bacterial diversity negatively correlated with operational taxonomic units and positive links in networks(P<0.05),whereas eukaryotic diversities positively correlated with positive links in networks(P<0.05).This study broadens our knowledge about sedimentary microbial diversity,community assembly,and interaction patterns on time scale,providing a reference for the sustainable management in aquaculture production.展开更多
基金supported financially by the National Natural Science Foundation of China(31902392)Key-Area Research and Development Program of Guangdong Province(2021B0202040001)+2 种基金Guangxi Science and Technology Major Special Project(AA23062047)Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)(SML2021SP203)Earmarked Fund for CARS-48.
文摘Given the importance of the intestinal microbiota in life-long health,increasing attention has been paid to ecological mechanisms that govern microbial succession.Both environmental dispersal and host priority effect play important roles in intestinal microbiota succession of aquatic animals,but their relative importance is unknown.Here,we explore the intestinal microbiota succession and assembly of Litopenaeus vannamei across larvae,postlarvae,juvenile,and preadult stages.We aimed to clarify the relative contributions between environmental dispersal and host priority effect on intestinal microbiota succession.We found that bothα-andβ-diversity of the intestinal microbiota changed dynamically along with host development.In particular,the intestinal microbiota assemblies were dominated by stochastic processes,except with the larvae stage.The succession of shrimp intestinal microbiota was clearly influenced by internal communities in the intestines of larvae,as well as the external communities in water and sediment.Importantly,the impact of environmental dispersal on the intestinal microbiota succession outweighed the host priority effect during the larvae and postlarvae stages.However,this situation was largely reversed during the juvenile and preadult stages.The possible reason is that,during the larvae and postlarvae stages,shrimp mainly feed on plankton from the environment,and their digestive system remains underdeveloped,the host recruits numerous microbes from the environment and selects specific microbes to aid digestion and nutrient absorption.These findings enhance our understanding of alternate effects of environmental and host factors on the intestinal microbiota succession of aquatic animals and provide a foundation for developing microecological management strategies in shrimp culture.
基金financially supported by the China Agriculture Research System (No. CARS-48)the Guangzhou Science Technology and Innovation Commission Project (No. 201510010071)the Guangdong Ocean and Fishery Bureau Project (No. 20164200042090023)
文摘Antibiotic resistance genes(ARGs), human pathogenic bacteria(HPB), and HPB carrying ARGs are public issues that pose a high risk to aquatic environments and public health. Their diversity and abundance in water, intestine, and sediments of shrimp culture pond were investigated using metagenomic approach. A total of 19 classes of ARGs, 52 HPB species, and 7 species of HPB carrying ARGs were found. Additionally, 157, 104, and 86 subtypes of ARGs were detected in shrimp intestine, pond water, and sediment samples, respectively. In all the samples, multidrug resistance genes were the highest abundant class of ARGs. The dominant HPB was Enterococcus faecalis in shrimp intestine, Vibrio parahaemolyticus in sediments, and Mycobacterium yongonense in water, respectively. Moreover, E. faecalis(contig Intestine_364647)and Enterococcus faecium(contig Intestine_80272) carrying efr A, efr B and ANT(6)-Ia were found in shrimp intestine, Desulfosaricina cetonica(contig Sediment_825143) and Escherichia coli(contig Sediment_188430) carrying mex B and APH(3′)-IIa were found in sediments, and Laribacter hongkongensis(contig Water_478168 and Water_369477), Shigella sonnei(contig Water_880246),and Acinetobacter baumannii(contig Water_525520) carrying sul1, sul2, ere A, qac H, OXA-21, and mph D were found in pond water. Mobile genetic elements(MGEs) analysis indicated that horizontal gene transfer(HGT) of integrons, insertion sequences, and plasmids existed in shrimp intestine, sediment, and water samples, and the abundance of integrons was higher than that of other two MGEs. The results suggested that HPB carrying ARGs potentially existed in aquatic environments, and that these contributed to the environment and public health risk evaluation.
基金This work was financially supported by the China Agriculture Research System(CARS-48)China-ASEAN Maritime Cooperation Fund,China-ASEAN Center for Joint Research and Promotion of Marine Aquaculture Technology+3 种基金Guangdong MEPP Fund(NO.GDOE(2019)A21)Key Research and Development Projects in Guangdong Province(2020B0202010009)Guangzhou Science Technology and Innovation Commission Project(201510010071)Guangdong Ocean and Fishery Bureau Project(20164200042090023).
文摘Sedimentary bacterial and eukaryotic communities are major components of the aquatic ecosystem.Revealing the linkages between their community structure and interactions is crucial to understand the diversity and functions of aquatic and soil ecosystems.However,how their diversity and assembly contribute to their interactions on time scale is unclear.This study examined sedimentary bacterial and eukaryotic communities in shrimp culture ponds at different culture stages.The most abundant bacteria were Proteobacteria(38.27%),whereas the most abundant eukaryotes were Chytridiomycota(27.48%).Bacterial and eukaryotic diversities were correlated(P<0.05),implying the strong interactions between bacteria and eukaryotes.Results showed that the bacterial and eukaryotic communities became increasingly similar on a local scale along with the shrimp culture.Only the eukaryotic community significantly increased in similarity along with the shrimp culture(P<0.05),suggesting that the sedimentary eukaryotic community structure is sensitive under shrimp culture.Co-occurrence network modeling indicated that positive microbial interactions were dominant.The homogeneous selection was the major driver of community assembly.Bacterial diversity negatively correlated with operational taxonomic units and positive links in networks(P<0.05),whereas eukaryotic diversities positively correlated with positive links in networks(P<0.05).This study broadens our knowledge about sedimentary microbial diversity,community assembly,and interaction patterns on time scale,providing a reference for the sustainable management in aquaculture production.
