Bacterial pneumonia is the most common infectious disease with high morbidity and short-term mortality.The key to the treatment of bacterial pneumonia is rapid clearance of the pathogen and effective control of the in...Bacterial pneumonia is the most common infectious disease with high morbidity and short-term mortality.The key to the treatment of bacterial pneumonia is rapid clearance of the pathogen and effective control of the inflammatory response.In this study,a disulfide-bonded hydroxyl-rich hyperbranched polyaminoglycosides of tobramycin(SS-HPT)was synthesized.SS-HPT could be electrostatically complexed with plasmid DNA(pDNA)encoding heme oxygenase-1(pHO-1)to form complexes nanoparticles(HPT/HO),which successfully achieved efficient intracellular delivery of pHO-1.HPT/HO exhibited broadspectrum antibacterial properties,good cytotoxicity,highly efficient reactive oxygen species(ROS)scavenging capacity,and effective anti-inflammatory properties.In addition,HPT/HO exhibited good bacteriostatic properties and effective transfection in the lungs of Methicillin-resistant Staphylococcus aureus(MRSA)-infected pneumonia mice,alleviated the cytokine storm and oxidative stress levels in the lungs,suppressed the over-activated inflammatory response,avoided the generation of lung injury,and improved the survival rate of mice.This study provided an antimicrobial gene carrier that provides a reliable target and effective method for the clinical treatment of MRSA pneumonia.展开更多
Liver injury,caused by factors like viral hepatitis and drug overdose,poses a significant health risk,with current diagnostic methods lacking specificity,increasing the need for more precise molecular imaging techniqu...Liver injury,caused by factors like viral hepatitis and drug overdose,poses a significant health risk,with current diagnostic methods lacking specificity,increasing the need for more precise molecular imaging techniques.Herein,we present an activatable semiconducting liver injury reporter(SLIR)for early and accurate diagnosis of liver injury.The SLIR,which is composed of semiconducting polymers with an electronwithdrawing quenching segment,remains nonfluorescent until it encounters biothiols such as cysteine in the liver.SLIR accumulates efficiently in the liver and respond rapidly to biothiols,allowing accurate and early detection of liver damage.The recovery of SLIR fluorescence negatively reflects the dynamics of oxidative stress in the liver and provides information on the severity of tissue damage.Thus,the specificity of SLIR,the fast response,and the efficient targeting of the liver make it a promising tool for the precise diagnosis of liver damage at an early stage.展开更多
基金supported by the National Key Research and Development Program of China(2021YFB3800900)the National Natural Science Foundation of China(51925305,52433006,52495010,52495015,52303381)+3 种基金the Natural Science Foundation of Xiamen,China(3502Z202371003)the Fundamental Research Funds for the Central Universities(20720230008)the Talent Cultivation Project Funds for the Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(HRTP-[2022]52)The study described was supported by grants from the project supported by the General Plan Project of Quanzhou,Fujian Province,China(2024NS009[to N Dong])。
文摘Bacterial pneumonia is the most common infectious disease with high morbidity and short-term mortality.The key to the treatment of bacterial pneumonia is rapid clearance of the pathogen and effective control of the inflammatory response.In this study,a disulfide-bonded hydroxyl-rich hyperbranched polyaminoglycosides of tobramycin(SS-HPT)was synthesized.SS-HPT could be electrostatically complexed with plasmid DNA(pDNA)encoding heme oxygenase-1(pHO-1)to form complexes nanoparticles(HPT/HO),which successfully achieved efficient intracellular delivery of pHO-1.HPT/HO exhibited broadspectrum antibacterial properties,good cytotoxicity,highly efficient reactive oxygen species(ROS)scavenging capacity,and effective anti-inflammatory properties.In addition,HPT/HO exhibited good bacteriostatic properties and effective transfection in the lungs of Methicillin-resistant Staphylococcus aureus(MRSA)-infected pneumonia mice,alleviated the cytokine storm and oxidative stress levels in the lungs,suppressed the over-activated inflammatory response,avoided the generation of lung injury,and improved the survival rate of mice.This study provided an antimicrobial gene carrier that provides a reliable target and effective method for the clinical treatment of MRSA pneumonia.
基金National Natural Science Foundation of China(52303381,51925305)Natural Science Foundation of Xiamen,China(3502Z202371003)+1 种基金National Key Research and Development Program of China the Central Universities(20720230008)the talent cultivation project Funds for the Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(HRTP-[2022]52).
文摘Liver injury,caused by factors like viral hepatitis and drug overdose,poses a significant health risk,with current diagnostic methods lacking specificity,increasing the need for more precise molecular imaging techniques.Herein,we present an activatable semiconducting liver injury reporter(SLIR)for early and accurate diagnosis of liver injury.The SLIR,which is composed of semiconducting polymers with an electronwithdrawing quenching segment,remains nonfluorescent until it encounters biothiols such as cysteine in the liver.SLIR accumulates efficiently in the liver and respond rapidly to biothiols,allowing accurate and early detection of liver damage.The recovery of SLIR fluorescence negatively reflects the dynamics of oxidative stress in the liver and provides information on the severity of tissue damage.Thus,the specificity of SLIR,the fast response,and the efficient targeting of the liver make it a promising tool for the precise diagnosis of liver damage at an early stage.
