The Yinggehai Basin is a unique NNW-trending petroliferous basin in the northwestern South China Sea.This paper mainly utilized stratigraphic,tectonic and seismic data by characterizing the geological structures and c...The Yinggehai Basin is a unique NNW-trending petroliferous basin in the northwestern South China Sea.This paper mainly utilized stratigraphic,tectonic and seismic data by characterizing the geological structures and conducting the geo-mechanical analysis to study the formation,evolution and dynamics of the Yinggehai Basin.The study indicates that the Ailaoshan-Truong Son extruded terrane is composed of multiple secondary extruded bodies.The Red River fault zone,located within the Qiangtang-Simao-Yinggehai mantle flow channel and basin zone,experienced transform-type sinistral strike-slip motion before the basin forming stage and formed a NW-trending extruded mantle uplift,which activated the Yinggehai basin.After experiencing the rift depression,fault depression,and fault subsidence,the basin eventually formed large-scale,thick sedimentation features with ideal hydrocarbon-forming conditions at the end of the Miocene.Later,the basin dynamically transformed and entered a period of tectonic superposition,reworking,and thermal subsidence.Superposition of the NNW thrust sinistral strike-slip fault zone on the northern Hanoi sub-basin complicated the basin structure.Since the Pliocene,the southern Yinggehai main basin has been transformed into an extensional dextral strike-slip environment that hosted numerous mud diapirs.The thin crust and high geothermal gradient provide favorable conditions for the large-scale accumulation of natural gas.展开更多
As a significant inducement during the develop-ment of oil and gas,the role of remaining recoverablereserves is more observable especially in the laterphase of development of oilfield.Depended on theproduction decline...As a significant inducement during the develop-ment of oil and gas,the role of remaining recoverablereserves is more observable especially in the laterphase of development of oilfield.Depended on theproduction decline method in petroleum reservoirengineering,a new model of predicting recoverableand remaining recoverable reserves has been proposed in the paper.展开更多
Small molecule aptamers discovered by traditional selection methods usually lack conformational changes upon target binding.This limits the use of aptamers as molecular probes for small molecule detection and regulato...Small molecule aptamers discovered by traditional selection methods usually lack conformational changes upon target binding.This limits the use of aptamers as molecular probes for small molecule detection and regulatory elements of genetic circuits.Here,we report a new method called capture and in vitro transcription-systematic evolution of ligands by exponential enrichment(CIVT-SELEX)to select DNA aptamers that can not only bind to small molecule ligands but also undergo significant conformational changes.Through this method,we select a structure-switching aptamer of uridine-5′-diphosphate(UDP).Taking advantage of its conformational changes,we first construct a UDP-responsive transcriptional switch by inserting the aptamer in a genetic circuit and demonstrate that it can respond to the addition of UDP and regulate the transcription of downstream genes.We also build a UDP aptamer-based biosensor that can be used for active glycosyltransferase screening.We believe this method can provide a universal platform for selecting small molecule aptamers with conformational changes and expand the use of aptamers in small molecule detection and genetic regulation.展开更多
基金the CNOOC Zhanjiang Branch Research Institute for providing the data and funding(No.CCL2019ZJFNO734).
文摘The Yinggehai Basin is a unique NNW-trending petroliferous basin in the northwestern South China Sea.This paper mainly utilized stratigraphic,tectonic and seismic data by characterizing the geological structures and conducting the geo-mechanical analysis to study the formation,evolution and dynamics of the Yinggehai Basin.The study indicates that the Ailaoshan-Truong Son extruded terrane is composed of multiple secondary extruded bodies.The Red River fault zone,located within the Qiangtang-Simao-Yinggehai mantle flow channel and basin zone,experienced transform-type sinistral strike-slip motion before the basin forming stage and formed a NW-trending extruded mantle uplift,which activated the Yinggehai basin.After experiencing the rift depression,fault depression,and fault subsidence,the basin eventually formed large-scale,thick sedimentation features with ideal hydrocarbon-forming conditions at the end of the Miocene.Later,the basin dynamically transformed and entered a period of tectonic superposition,reworking,and thermal subsidence.Superposition of the NNW thrust sinistral strike-slip fault zone on the northern Hanoi sub-basin complicated the basin structure.Since the Pliocene,the southern Yinggehai main basin has been transformed into an extensional dextral strike-slip environment that hosted numerous mud diapirs.The thin crust and high geothermal gradient provide favorable conditions for the large-scale accumulation of natural gas.
文摘As a significant inducement during the develop-ment of oil and gas,the role of remaining recoverablereserves is more observable especially in the laterphase of development of oilfield.Depended on theproduction decline method in petroleum reservoirengineering,a new model of predicting recoverableand remaining recoverable reserves has been proposed in the paper.
基金supported by the National Natural Science Foundation of China(32001037,22176035)the National Key R&D Program of China(2020YFA0210800,2018YFA0902600)+1 种基金the Natural Science Foundation of Fujian Province(2020J01491,2020J05120)Fuzhou University Research Fund(GXRC-20033)。
文摘Small molecule aptamers discovered by traditional selection methods usually lack conformational changes upon target binding.This limits the use of aptamers as molecular probes for small molecule detection and regulatory elements of genetic circuits.Here,we report a new method called capture and in vitro transcription-systematic evolution of ligands by exponential enrichment(CIVT-SELEX)to select DNA aptamers that can not only bind to small molecule ligands but also undergo significant conformational changes.Through this method,we select a structure-switching aptamer of uridine-5′-diphosphate(UDP).Taking advantage of its conformational changes,we first construct a UDP-responsive transcriptional switch by inserting the aptamer in a genetic circuit and demonstrate that it can respond to the addition of UDP and regulate the transcription of downstream genes.We also build a UDP aptamer-based biosensor that can be used for active glycosyltransferase screening.We believe this method can provide a universal platform for selecting small molecule aptamers with conformational changes and expand the use of aptamers in small molecule detection and genetic regulation.
